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[ [ R R R R s s s '''111<<<FGGQPPZZZdccmmlvvu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: aaca284f589e42fab5a3404ab40a1518Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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6 R 5 1 H T ? F D N A d , u | 0 i \ e y & Y @ P U T < i y#* 3 6 X > ? d O J Q ^ g > F ; y! > A Z _ f u o I a+- r ~ U " Z E l K { * + " I | } R TQ 8 d z x i # H i G xU F QAo _H -y |)Ic U ^ s 7; B @ @ B h W ? I5X PJ ; OI Bu n Y C Ii %7 \ Y gc&9 ~i 'Yd y ; 3 08 n \1XVw3 i - 9 u ) Zx3 ) ' m^G O@ i r q @ l r J B gp ^ @A$ H T_! } E, oJ`J ' Q ` P2x >BT ] /ZB 5r 1 Z ATTTGTTTAAAACTCCGAAGGTGAGATATACAATGTTATTACGAGGGAGCCCTGCGCATACACGTCCGTAGCTGACCCTTAACACGGGATCCGGGGTAGCCGGACCCACGGGCACACCCCTTAGAGGGGCCCCAGCGACACCGTAGTGTTCCATTAACCGCGGGTCCCTCAGAAGGATTAATTGGACGTATGACTTTTTCCTCCTGTTTGCGGTACGTAAGAGGGGAGGGGGCTCCCTCGCGAGGGCGCGGAGCCGCGACAAAAACATTCTCACTCAAGGGATAGAGTTCATGATATTGAGAGTAAGGGACTATGTACAGAATATAGAGATTCCCTCCCGGTGGGAACACCAGCTGTTAAGACCGCCGCAGGCACATAACTATTATCCCTTCACATACTCCACGGGAGCACGATATTCCGTGGAGAGGTTATACACACTTCGTAAACTATCACATTAAGCCGCGGAAGATTCGCGCACCTTCAGATACTCTGGTGGGCATGAATAACCGACTACTATACGAGGAGCTCTACCCCCTCATAGTTATATATTCGCTCCACGAGATCTTCGGGATCCTGATAGTGTTTATGAGGATACTTGACATATAGGGACACTGATATAGACTCATACTCACATATAGAGGCATATATAAGAGGAGGAGTCTCCCCTCGCTCGAAGAGGGTTCAAACTGAGACACTCAGTAGTATCGAGCAATACTAGACTCGTGAGGACACTCATTATTTGTGCGGAGGTACATATCAAATACGCAATACGCGAGTCGTACTCCGACCGGTTGTTCTCTCCACACTTAGATATCTCTACAATACATGTGTTGAGGCTGTCTTCTCTCCCAAGCATAGTGTGAGGTGTGGTGTATAAAGGAAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:09:41 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS S BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,K X DATA
,K X =1 DATA ,K X DATA ,K X ] DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F P1AM t i P1RL t b P1WD t p P2AM t ~n P2BA t t V P2RL t w PBAS t t J PBAS t t G. PCON t t _Z PCON t t [ PDMF { PDMF G PLOC t T PLOC t N PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% F SCAN 3 SMED
SMLt SMPL p SPAC AUc SPAC G SPAC AUc SVER 4.0 SVER H SVER , , n Scal @ Scan 3 TUBE B4 Tmpr < User phAR phCH G) phDY big phQL c phTR phTR ́ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS S BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ,K X DATA
,K X =1 DATA ,K X DATA ,K X ] DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / n GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS F P1AM t i P1RL t b P1WD t p P2AM t ~n P2BA t t V P2RL t w PBAS t t J PBAS t t G. PCON t t _Z PCON t t [ PDMF { PDMF G PLOC t T PLOC t N PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% F SCAN 3 SMED
SMLt SMPL p SPAC AUc SPAC G SPAC AUc SVER 4.0 SVER H SVER , , n Scal @ Scan 3 TUBE B4 Tmpr < User phAR phCH G) phDY big phQL c phTR phTR -'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWir;4r.u:!rYWHw_km<|Wq_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{cQ&3fp^VXYCqsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOj1 ~>e4d|5H_&kl5K02iL(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0Fk(I=fd\[ ;VuJX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0