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-fz5 z z } v s @)KpOK#vE u y s k y a n [ c c b ] o v f b q P o R d \ N U H H X Y ] W d e h K W Z C H S \ a P Z l J O [ Q d a c T f V W Q U o F c ] X f c [ l ] Y l k d h U R P f ^ d D P W ^ b [ \ h g x t j } Dq*C[j\.X$ ~ v J] ?o]^erd8sH- { w g v p v ` ] ` o r k r { a l j i R V [ S T j f g j h R ] _ a V R [ \ \ e [ c a P U d k n h c _ L T l ` g d L N k ` O ^ M c V e ] b J Z Z < U e G 8 O Z S ^ U U _ a Y g [ V o c b J b2 | Y i g \ Z j _ ^ ^ _ ` b o r ` U ` Z e m I e k ` D Z [ W A ] X C D C I R D S D E F [ [ ` d S R S W h a Y [ j l W X Z T O N ^ \ O c E S d p S 9 K Z P K F ] J _ P g D [ ` [ ^ ^ ] ] W Y U [ I N M I 3 : F S K W ] X Z D L B W Q ` Z J S U ^ Z L D T P a N b V m k k Y t _ w [ Y c w \ o k o a { m a a i m p l s t z s { /K=D~f}o9@! o f r i y u ^ h ] m ` v l w k o v n ] r } S S R j P { q ~ j y j l q b m y v d r j q j i q h w { o | v { c v o ^o Nf}hW15Hm\}xu5`,% ~ ~ l ^ s y w r ` t c b [ j p l e S t s n a q n o r z ..LB e s b q n p \ e X I \ O V Z T b L Q Y L S ` Q J @ [ V R Y 5 M K 8 9 K E T X A N I R K _ T T T b N K N C h 7 G I Q G T H b g Q ` K m W a m u i o ;PrT# u q e k h [ g L U Z Y I V Q F I D H @ K ; 0 ? F H . A F 8 4 3 F @ 6 1 O < J C 0 F \ a M T W A 7 @ B > 5 8 1 B I K H G ; I D K O Q F C B P J G = K D D W E I D K N [ > 8 F D D V F X G K L [ W Q T R Z U V C S b K Y C Z S Y ` b a c c [ W a y i Xn &@K9#+G" x f Y b f \ t Q [ F Z U W Q T b X U N W J Q _ d M h S W Z P Y j J B Z F Y O D ^ P Q \ X O L L Z J K E > J M A E ` L U O B N S G Z ^ J R F K O T K J L U 3 Y J P R E d I O I S H X J d ` c X K W J U v a X l e [ m ?o 0%958F]0 gA | | t i q ] c d X a h N R Y P \ ] b I M ` H @ _ U Z Z D _ F M E c R G c G Y I E V X G K R M h P c Z ] V K _ ^ Z O Q b S R [ T c S ^ Y ] ` X g Y Y a ] k u ~ +c %kJ& !3EXsL>& x p l n Y Z Q m X @ c M C \ V Q G H H ` L U U B Y ` T K L P R @ I D P a O [ X 3 O ` O Q 8 V X H ; B V < H K B M ` G P C R X g b ] \ [ R V Q ` a _ Y Q R R [ c t ^ c d g c m [ R e m l d } 6Ab .66/Ibp`?P1% hX= } u j r l X ] [ k l k b J c g ` _ d ` V ` e ` x s x +*CYILA4 x ~ ` _ Q K O Y O K T ^ M Z ^ ` X q n c a ` z [ _ u [ i u r ] a X I Y < g ^ f f ] a j j o n *FqqS' D| V/
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n ] Z ` A C M M ; . @ D M D @ F D : G & ; C 7 4 M 3 . > ' # 8 . 1 $ - 6 D K 8 6 = % 2 * B , D : S J 7 A = F 1 ) M D 7 7 M L M i l w
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%; } } h ] Q U B > P H E 3 3 4 9 / = . 1 & + 0 5 5 * , , - 4 C + < 5 ) ) 2 4 % $ / + * 8 2 6 B @ H 4 ' 3 0 2 3 * 5 < F E E F G H J W b f ~ t u ~ ~ w i q d t h I C 7 F L 0 0 A , 5 6 ' 3 4 , ( A ) ) % + . 5 ( - - ' ; 1 4 ( / * ( / ' 0 4 7 . / / , . 5 / - + 7 % 6 ; ' , + ! / + " " # ) $ $ $ & ) # & - , - ' + 2 > ! & ! $ & , , # 1 / , ' # / 6 ! # ( % / ) 1 , 0 . : , " 3 & + $ # & - # ( % - * % 3 ! - ) & ' " ' ( : $ # , # * ) 9 ! / & 6 # 2 ! ; # % 3 & > ? F M d ? ` U L g } k z r d k m W ^ X ? E F ( C @ 4 0 0 3 9 1 , - * % ' & & > - , & ( . 3 A 8 4 G < A F b Q ` g h V k r p q x y f r n h P [ L V U B ; ? B 3 / - ! & + ' " 1 " * & + " " # * $ ( . & + " + 0 % " ! + . % ! 8 # ! ( " % , * $ % / 0 / ) # * % ! 2 " ) + * ! # , + 1 + 1 - % , 4 ) ) ( ; # 8 3 - - , 4 @ ) C 5 I @ G J V \ l _ h y d t { m } n Y l u j ] ] \ F D G @ = U ? ? C ? % . * - 5 % 2 # # ) # + ' % $ ' ! 6 . & + % / - ) $ + . ) ) 0 - 3 2 ; D 8 @ E 9 I Z G S ` k o j w x | | v w ~ } d w w j y q t v o v ~ n t { h g e O R N I I J ) 4 + 4 # ! 1 + + & * 7 $ . ! / & 0 * " % % 6 # + % # " ( & % " # 4 * & 0 # )
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[ [ R R R R s s s '''111<<<FGGQPPZZZdccmmlvvu}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 51ac99266e4946ec9ed7c983f986f4dfSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_08-39_0774 3130Seq 12506 12506_Costion_K1316_matK_R jmadera 96-Well
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z) 6 6D S#s>"W[ # ; d O*D`Ef \g3 5 d ^ T#C V" b CU2n 2 TTGATTGTAACAATAGTGAGGAACTCTATACTATTATAAGATATTGTTACGACAAGATACGCTTACTGAAATGTAGACCCTAATCCTCTGACCCTTGGTAAAATCCCATTGAGTTTGGGGATGGAAGGATTGATCGACACTTTTAAACTCAAGCGAGGGAGGTACCTAGAGACGTATGGGTAGCACGAGTCCAACGCTCCTCATGAGAGGAAGGGAGGAGGGGGGAGGGGCCTCGCGGGAGAGGGCGCCGGGGGGAGGAAGGAAGTGGCGCACTAGAGGAAGATCTTAGAGGGAGAGGGAAGTAATGGCATATGGGTTGAATATATCCCTTCCGGGCAAGAGACAACGGAAAGTTGTATGATAACTTCAGGGAGACGCGGAATTATACGTTTAAATAATCCATTGTATGCGCCTTTCAAGTGGAACGAGTAGTTAACCTTGGTAAACCTACTACAGTGAGCGAGGGAGTATCAGTGTATCAACAGTTACTTTGGTGAGGGCAGGGTAACGGACACCTGTGCGATATTCTCTACCCCTACATAGGGGTATACTCGCTCTATGGCACGCTACAGGATATGGTTAGCATGAGTGAGGACAGTCGCACACAGAGGGAGAAGGCGACATACTTATACTCACACATAGAGTGTATAGAAAGTATGAGATTCTCTGTTACATACCCGAGGGGAGAGTGTCTCCACTCGGTTACGAGAGGATGACACTCTTTCAATGTCACTCTCGTAGAGGGAGTCTCTTAATTGTGCGAGAGCGCATCCCATCTTATCGAGAGATCGTGCAACGATAACTCAGAAGATCTTGTGATATAGAATATACTCTTCATATAAATTGGTGACAGAGGTCTCCTCTGGGGAGGGTATAGTGCGTGGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 10:09:59 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA - Z< DATA
- Z< > DATA - Z< DATA - Z< O DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS M P1AM v pb P1RL v iv P1WD v wN P2AM v & P2BA v v P2RL v ~: PBAS v v Q< PBAS v v M PCON v v f PCON v v b PDMF { PDMF M PLOC v [ PLOC v T PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT ' RUNT
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1 Rate RunN " " S/N% M SCAN 3 SMED
SMLt SMPL p SPAC AUO SPAC M SPAC AUO SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH M phDY big phQL c phTR phTR U This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA - Z< DATA
- Z< > DATA - Z< DATA - Z< O DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab l FVoc [ FWO_ GATC Feat / / , GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS M P1AM v pb P1RL v iv P1WD v wN P2AM v & P2BA v v P2RL v ~: PBAS v v Q< PBAS v v M PCON v v f PCON v v b PDMF { PDMF M PLOC v [ PLOC v T PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
RUNT
RUNT
1 Rate RunN " " S/N% M SCAN 3 SMED
SMLt SMPL p SPAC AUO SPAC M SPAC AUO SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH M phDY big phQL c phTR phTR qCfkNR(oBj'~;Hk@*w&RA#] lM6[/:){hV(wX>?7u%#F7Np1mj;RJJ4R,9X PMF9zH'zAuR)x|ec{PG6x|W"OKOfx&i1PBEe>2flV:2/dmm YJX?/WZ#}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvM(\_1B(hHR(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4 `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H2gQgKRd+ol{+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~bea6:!.tk}Y=52rR~o[k7MLr92U*a! DATA - Z< DATA - Z< O DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab l FVoc [ FWO_ GATC Feat / / , GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE / LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS M P1AM v pb P1RL v iv P1WD v wN P2AM v & P2BA v v P2RL v ~: PBAS v v Q< PBAS v v M PCON v v f PCON v v b PDMF { PDMF M PLOC v [ PLOC v T PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT ' RUNT
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1 Rate RunN " " S/N% M SCAN 3 SMED
SMLt SMPL p SPAC AUO SPAC M SPAC AUO SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A5 Tmpr < User phAR phCH M phDY big phQL c phTR phTR