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y a V H F ? E 2 / < 7 2 * - ; / , - . 7 & 4 2 % @ 1 > : Q 4 < C 7 L L 0 5 2 % < 6 4 6 = I Z N x | n b Y U ? . 1 7 = + ; 4 " ' 8 + 5 ' 4 3 = 7 < I C = 0 ; 8 7 3 % 1 ( 0 ' + ' # , 3 2 1 # : % 1 = 9 M O T g } v x p ^ K N H . 5 ( 1 * * 5 2 9 > + 6 ; . / < B P @ D I B M B V K Q E J A B K M M B @ B > 3 7 / + 0 * * / 8 @ ) + ) ' * < # % ( H : % ; % / % 5 " ( . A A 2 F A i m | m e W F N D D 3 8 C B A J c h | _ ^ Y H 5 A 4 D 6 5 : 3 9 / D 9 6 * # , E = @ J M E < R J _ Y M 9 H E V > 3 8 $ , 2 0 8 8 = ) : - 4 2 X K H L > Q 2 K 0 8 : > 2 / + $ 6 . 4 2 4 1 * ( : 6 3 = < C < 9 = G ? J C ` N < I L @ @ 1 7 B > ? G H < F > 1 F > C = 0 ; H F B @ 6 J 4 > C @ 9 7 L L : - - . + C B 2 F H 3 ; I ? @ H E A ; R G \ _ w k h A R ; < ; 7 2 , ( . + 0 D ; 4 > ; I C : A 9 J 1 M / > - M C H [ G 7 K K D M F O Q Z ` N V R U O Q T ^ K J : C I F 5 < 9 4 D H + , 0 @ ' * * : 5 . > C : A ] j c f Y I L F F > > H 1 8 T Z } | j k l X L I E $ = 6 9 ) . : & & ! ! ) ' 1 0 3 = ' 9 1 ; 9 A . ; 8 ; 7 B G 9 D E D T @ = ? D E : = 4 ! $ , . - : ? 2 ? ' ) 4 ( 4 : & 1 7 ' < / ) 6 4 - < I Q 8 N 2 5 K 6 > D > . 9 < B 7 V O g } } ~ { u I j X I ; Q < C F O ? = H @ E ? 7 [ > G > ? & @ K = P Y K R J H P S U D _ a _ b l | n s ` k U T B < M P L H Q P I N U H ^ T K H G > > > 8 D L 9 E ? C N I B @ 6 F ? 6 5 ) @ * > ; H M > N c j z c k W I A I 0 > > 4 4 & ? ( 5 = 3 8 ( H D C , 4 6 ( < ! 4 + " < & ( , < * , + ? G " , A ; = 4 5 F B A = # 1 B D : 1 < ? 1 A , F 2 9 3 9 9 = 5 4 8 8 - 4 - 7 ' & 9 ( 0 # + 3 5 5 # 9 4 1 5 1 / 7 7 " : 4 ; B @ F V m [ q w x Z P E O I < E D ? : \ i d _ [ [ Y C 8 H 8 8 6 # < ) 3 9 / < : - * . > 3 ; 6 ( $ 6 ( ( - ' $ + B 9 T Z c l z [ Z _ = C P N @ E 3 < ' : > = / @ 4 % : - 2 " @ > 8 ; / - - 9 6 > . 5 > = C 0 4 , = I = 1 9 7 H : 3 2 9 B 1 4 @ > 7 E D F N c s y | S Q N B ; / # + % ( ! . & " * & $ ' 5 ) 4 5 D E Q X p u ` a G ] \ = 2 5 B F E F ' @ 9 G E R A 8 N ? ] B J V U u a | s f N J : @ F B 6 ; . 7 = < * E A 6 3 5 > 2 3 0 A E / 9 C B B K O < ; Q G O P T X u q u e _ c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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 91172c58babe41c6954a5f19d74ffc01Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-24_06-43_0773 3130Seq 12505 12505_Costion_K1316_matK_F jmadera 96-Well K1316_B1KB_3730_POP7_BDTv3.mobSeqPop7z )7GWfrz~qbRA/ 3Mj
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H[DGTTATCCCGTCTTTATGCGGACACATATAAAACACTCTCTTAAACCAATATAGCATTCGAACAACGGACTACACATGCAATAAAACGCGAAATCCAGGATCCATTGCTACGTAATCACACCATTCCACAACACATCCCAACAACTCCGGAAGTATCCCTCAGATACCACCACAAACAATAAACAAAAAACATTCCCACTTTTAAAATTTTTGAATACATGAAAAGCCTTTTAGTCAAATTTTTAAAATAACTTCCGTGATCCACCAACTCGCACGTTCACCATGCTTCCACTTACTTGGAGAAAGACGAACACACTGACCCCCCACCTAGCCCCCCCACTTGTCAAAGATACCCAACAGCACCACACAGGACCCATCCCAACAAATACCCTCCTCAGCCCCCAATAAACAAATAAACTCCCCACTATCAGTATTTTAATCCTCTAGAGCCTCCCTAATATGAATAATAACTTTTTTTATTTTCTGATAAATTAAAAAGTTTTCAATCACACTACGCGAATGTTGAATGCCTCGCGGGCCCCCCAACTTTGGGTTACGAACTCAGCATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-24 08:14:27 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS yW BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA - Z( DATA
- Z( > DATA - Z( DATA - Z( C DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ w LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Ms OfSc P1AM 8 p c P1RL 8 p _f P1WD 8 p hF P2AM 8 p q& P2BA 8 8 u P2RL 8 p l PBAS 8 8 O PBAS 8 8 M PCON 8 8 ]. PCON 8 8 Z PDMF PDMF M PLOC 8 p V PLOC 8 p R PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT +. RUNT RUNT RUNT 8 Rate RunN " " S/N% Mk SCAN 3 SMED
SMLt SMPL SPAC AY- SPAC M SPAC AY- SVER 4.0 SVER yL SVER , , Satd Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH M phDY big phQL c phTR phTR 0 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS yW BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA - Z( DATA
- Z( > DATA - Z( DATA - Z( C DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / r GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ w LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Ms OfSc P1AM 8 p c P1RL 8 p _f P1WD 8 p hF P2AM 8 p q& P2BA 8 8 u P2RL 8 p l PBAS 8 8 O PBAS 8 8 M PCON 8 8 ]. PCON 8 8 Z PDMF PDMF M PLOC 8 p V PLOC 8 p R PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT +. RUNT RUNT RUNT 8 Rate RunN " " S/N% Mk SCAN 3 SMED
SMLt SMPL SPAC AY- SPAC M SPAC AY- SVER 4.0 SVER yL SVER , , Satd Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH M phDY big phQL c phTR phTR 0'"sIR*"$JQ`Q5f|{ce+dr6>svpkx5yu(p4-dId_$_;zay@Q>ko2Uz| Q-M2"ZwclQ[vL}W$620e:jA:>y7LIG!Hgn+1Ab;*5" ~Jz!#fn2+Xb[l"G>H1,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM{3!dX!%~Afzv*Cy KQI@'.%Nz7R@X`@kjJ}B~xGH$i*8j|]]u.ibu:,n_SUgi8i(=l27&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/Ygn`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0t[XAn@Bg-'!i8- Qw]qH9sKe6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t DATA - Z( DATA - Z( C DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / r GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE
LAST ~ ~ w LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Ms OfSc P1AM 8 p c P1RL 8 p _f P1WD 8 p hF P2AM 8 p q& P2BA 8 8 u P2RL 8 p l PBAS 8 8 O PBAS 8 8 M PCON 8 8 ]. PCON 8 8 Z PDMF PDMF M PLOC 8 p V PLOC 8 p R PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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RUNT +. RUNT RUNT RUNT 8 Rate RunN " " S/N% Mk SCAN 3 SMED
SMLt SMPL SPAC AY- SPAC M SPAC AY- SVER 4.0 SVER yL SVER , , Satd Scal @ Scan 3 TUBE B1 Tmpr < User phAR phCH M phDY big phQL c phTR phTR