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"                %                                 %                                                                           !                                                                                                                    d,,,III^^^{{{&&&999DDDXXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRB$(**+*(''%$#"   [[[RRRttt#((233===HHHRRQ[[[eddnnmwvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f7b3a165b3f64d4085edbe14d037f132Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-23_19-34_07663130Seq1250412504_Costion_K1315_matK_Rjmadera96-Well K1315_A8_RKB_3730_POP7_BDTv3.mobSeqPop7z7sfm%UMK j 8 : c \  A :$ S ct}jn,22l  a  K b'j; t<s*{L.L`gcYMC;6320)zqkgaYSVd-m:[q||wv&i9 F  M ~ P :v1%78JYVv8tsw}lJ+  "zh]`u\\KKXn6"Lm~wyUgt\ yG6ezze:0s<&*8A5 9$_UlsE5@[|W:u6XoR yb_GGViv}uY=&  3Ias{I )4~A2du$0`TI*)Ez?XFp<3[u12n|^TSN=!<\i_Ar4n: '4>EKR[ce`Q;$7Tkvtlgk|Oa"42P#md$8RWx& 5_(eD ?kKlKq3U5XS.0DM38r/  )f/+Bqt@ ** c]U6);gv k5;q|)ys6.SB4u%2K~=(c{"S|3i9Ss*"J~@wn9R<]!,+URwrC[%(Hh{}kN-"=]~4h'LbbKLvxK^UENBXx|V.!^m)G `gdu#Q& "1Il[k4}b>$KkxkI e ;D%qH1%&09BLU]_XE, .WzxS):L:pOJnD&4g-o): &    6M^how-~p= &a]7aqcz+^1E0M{w|pM* &EcxyfL0  ,<FKMTkYGf;MdL5 0oM2i V{o45  <fmC ! 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( ` #FlI#uy!?Fr<rONOyL;;P 86h:<CR+29#yE6#d#  \&8&7B  -H ?C  $ ]Q.8SuM{R]CRR3Z?,L,b?~Q`a) ,.C6:6U{*7n1y{f,s:,ULE%=bp!8{c H|DR&*3G.;qlpPVlp,zuj]| t,:+{a?qSPfu<`d,G _G X2*xw ,oQD)WrkXS 6|KNy{k~{H Y1'Q Il @[YYjeG5.WVAK8lOt;v:P)atQ)s9CI!^ NZS"QE9~K8I>VFMfer^[9yIR75}F;V3pO\@.&kCB U OUEgg0]J7;|/8] A_GGAAAAATAGGCAGAGTTGAAACTACGTGGGTCTATAAACTCGACATATGATCTGGGGGTACGATGGTGCCTTTTAAGGAGACTCATATAAGTAGGGTAGTGGAGCCCCAGGAACTACTCGTGAAATCCTTTTTTTTTCGAAAAATACTTTACCCTGAAGCATAAACGGGTAACGGAGCCCTCAGAACCTCTCCCGATATAAATAAAAGCAATCATACTCATGATACTCGCCCCTGCGGTGAATCGGCTCCACCTACGGTCCCTCCAATGCGAAGAACGTAGGTACAGCTAGTGTGTAATCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-23 21:05:34 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #vFDATA #vF+DATA #vFrsDATA #vF_DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM/^ \P1RL/^ P1WD/^P2AM/^vP2BA//P2RL/^PBAS//PBAS//PCON//PCON//PDMF{PDMFjPLOC/^BPLOC/^PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT "RUNT RUNT 7 RUNT  Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #vFDATA #vF+DATA #vFrsDATA #vF_DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&FVoc&FWO_GATCFeat..&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM/^ \P1RL/^ P1WD/^P2AM/^vP2BA//P2RL/^PBAS//PBAS//PCON//PCON//PDMF{PDMFjPLOC/^BPLOC/^PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT "RUNT RUNT 7 RUNT  Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTRP'd}|(H-'Icf]h_m7^d`7Y{v]qoUYoOyl}$r&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hDM=VrTH&zqru+!hs|O1i~?z;{W8<)mbo)6s6IxO\JpSbwEiv5&o58J.:)'C#x5ots/H1C:2#%=c/a}ayHbc\S.Tqt6IEUYC~n{[._LbR/"E.Om,NeLVWY+a:WkeC.%oh^5Q1V?|&@8je]i^mcR=TGWAU '[/bzop2iv4*un,T(k"T'ogLc{Xm|3HF)SE8n\qoA(&BFyENBrjylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfb;b%^!s]zJB)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jxAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTdd4B1PtB1PtBCTSBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #vFDATA #vF+DATA #vFrsDATA #vF_DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&FVoc&FWO_GATCFeat..&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISSP1AM/^ \P1RL/^ P1WD/^P2AM/^vP2BA//P2RL/^PBAS//PBAS//PCON//PCON//PDMF{PDMFjPLOC/^BPLOC/^PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT "RUNT RUNT 7 RUNT  Rate RunN""S/N%KSCAN3SMED SMLtSMPL pSPACMSPACcSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTR