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9x } m a g o Q j i b { [ j x KmML3F z u | ] c j p g _ i v k m U a U ^ V Z P ] } y O p u ZfX =*G^ z s g \ j y U } v s c | v | v h V l l i l i b h u _ _ i l Z e ^ l q u o f n -{RdK1 m!%l*w"}6V v Z h ] \ r \ = P K g W : C E V N F P ; E = : G ? C L G ? S d Y ; I ; L M Y G M [ ` f Y \ g O B L d Z g h d K Z u b q r z j t w m \ U d ` W W C K M U D U W G F f [ [ Z E T \ S d \ [ S E N J X C c n *v.x,I) v v Y U o ^ Z B S R S S m I f S C S Y D F 9 Y X L I G 8 U @ P = 4 3 9 K R E W 8 K R N e J P b Y ^ V C O O f v t ,.i{y 2 } } } Y j _ ` d _ I N > ? V S M E a O Q P ? C P F X U X G X K O T ] a ] f ^ j v m m e ` w _ y k S g R Y S O E W ` [ Y V d ` F ^ J G C G ? K N I E J U O U N : J C @ = > : O 7 3 L E 3 J F V V ` ` W F I 6 Q W 9 G T S I K n E P Y W J ^ F R U V K ; C > 0 3 7 ' L ' 4 G 3 T 9 C @ D U X T 8 9 Z V P I 9 H G J E ? Q W ? O A . G ? @ : 0 K M H A 6 3 2 3 K E O P 8 M P M 7 E = $ 2 ; 5 9 @ 7 > F 0 H G = D C N G : @ T K 7 = B B J N : A B W ; 9 N G L L ? E K C 0 A ? F ? ? E : @ P N q U K J > R N S _ K A O U O N O H 5 @ = T X [ _ ^ n u DsGaV- z { ] Z l O b l u c q w x y 3sa?L Br- f ~ ] s j g a Q b a V P I Q ? \ X u [ V b _ R V M X V H Z d P C J I F H > G ' @ ) # 8 D @ A : 7 F < > B M < > 4 . P 6 / 7 7 ? ; 6 1 * 4 ( A @ # $ 4 ( 5 D @ 8 5 2 C D D R H ] K G ` H J ' C B * Q > K B A N < T 7 B 3 = 6 N G < M J : H < M 8 3 = 5 1 : > > ? ? K < 4 S Q K D B P H J 3 P K _ P S R M K ? = O W I L B ? 8 < 3 3 1 . + $ D 9 9 . I A & @ + ; ) 3 ' % / - + C 2 4 6 3 3 : / . , 3 @ @ . + 7 ; 9 < = < I N 4 D - D K @ H ? E 7 M I C Z : = R h Q N j d d z F~4D6.m | p Z r d W Q V R Q [ F P M V C O T C Y = U ? I U B P D S K R F T N W O d T j P D K - B ? E D K B O P : G N > ? O @ : I > ? [ K M ? 9 U 9 K T Q U R ^ I K ` G _ a V M9lW } k c ` j F W R D X _ D R . K K [ B I ^ Q W Z f c j q agI_{~i9 c0|D!A1
]O~9 q g } _ d n n n e D d E 5 C J F Y P I C T N f H I R N ^ S P U I X C _ U L > I J = @ @ 6 W Q G 8 H Q N N T F Y J G G L I A ^ = W ] W H K O P R N Q Q S O \ d S [ Z ` n &WW20 Y] v t b j u \ q h { ^ s ~ @O;B%e\6~1 k u a V [ T T J S G M N Q 9 0 6 8 9 . 0 L L W D B B H L 7 : F S S 7 O J R ] \ X c N D k S I V ` _ S a b c ` \ _ W R ` d E g h e | } b Xf_@O <b^A vT z t o o P f e h O V f ] h U [ g u s k a y 2r$ y~3TH p p [ S b S T N ^ a Y V T H J m E L C T ` k o p q J'Bi- Qn# p v z n l d T Q M U W R ^ W ] Y R g : > G T J J G M 7 8 D G @ J F ; 2 A P 2 4 C N > B < : L , > > < 8 B I 3 < ; 7 A O A ' I V I : 7 @ 9 > 8 A 3 1 1 . C < F F G = D ? J V ? > Y S K S R n @ k Y U } b v &X$(U d b d [ ^ R b \ L E N T K K > K B F G F 7 S 8 M Q A = C B < ? ; T c U Q a R Y E W ] J O Q U Q R Z U f g i } y 3&\ +l w { ] Z j d ` S F W H N O F W N N F O H R R A K I 6 G N B D J ; ? > E ? 2 7 3 1 < = 0 3 % < @ ? . 2 @ 5 7 2 6 1 % & @ 2 1 6 . : : 7 = B 2 = 0 H = : 9 M > < > ; @ G @ G @ < M D A \ C J L F F S N T P a a n U Y m a l )QC,R-4$<V|><~/ j l n P H V ] Y h Q S b o P _ d Z ] X V u n w } fXet;k s h s J O U R Y ] Y F ? R [ Z O I M T = \ T T T U W a [ M h Z H b U l n h Y e _ 9w"9v[4i0 Y%Y1UpW _ d, f a _ Y M T _ i B : E C I I J 8 J K G b B C O J ? > D = K C N M P ( A H B = 9 " 8 7 ? C G % L = A < ? G > 6 @ D J ? 7 4 A J > F K 1 < B < C 8 ? E H N E > , I D I E C A a H _ O M Z X [ 'eSu"MpG &| KBt %i> } s v c g g r S ^ S J B G G U A Z ` L \ S 8 O T G $ _ M K ; + J L E C J R N I J V B M G J Q m U Q H N e J V ? E N ^ j N N I V K T Q = > J G V F J V < ^ I 2 _ ; K @ 8 N - C R E H P S K L Q : G ; < K - 3 > G F 7 4 G : . 9 = K H J 4 > I D R E A 5 5 Z ^ R N - 3 8 ; ? * = 2 3 - ) ! / 4 - ! 2 ' , ) 2 1 1 - " 0 . & + 5 4 * 4 < G / 4 , D + - 2 1 ) ' 1 * ) % ? * ( 4 ? 4 . : $ ? < F 9 ; $ - ( . @ 0 + . 5 1 1 ; = 7 : 6 / ? . ' ; 3 / 4 A $ ! - 5 9 1 8 5 @ A = C 9 8 G @ = K 9 . 6 9 / = 5 5 9 2 > / 6 D 5 ? # @ > + * 1 B = . : 8 7 B 0 9 6 K 8 ( ; I E 5 8 C @ J ; 5 M 0 F W a U T K G B @ 4 G A ? G ` 5 3 > G = D ? ) 6 Q 8 J N ] W m '[ =v]naX$^EUod(a|:& 9h`3 5{ a { d j e u c x q k v e s { z { ~ z s z v o } n { r t c q v ~ j b i t t m s _ l h x o g n q d m m j \ M S K ] H W I \ ] Y W q X I [ a ` J Q R ] G ? O J > D B M F 6 ? @ * P I O H N M C = V < I @ A F 2 F C Z H G P ] M @ Q H H Y _ \ Z < V T U D K Q T H > @ C K D G 7 8 E J O , I E : S = < ; = R L C C U M M F = = I P ^ E W X X N N e Y d L l -Pl+ n v c p i M _ P N M A J J D J @ G D < & K 4 > \ C 6 D ; E 7 U O ? H C V A H S Y 1 G F M E : S D K U X N L K Z ^ Y H V G H H J O L B 5 E C 0 L @ 9 # E 8 / / 5 E + 3 ! . ( 1 7 ; . ; G A 3 0 @ F A + - F 3 ; C A D < > - ? L @ A 3 9 4 5 > B K @ N 6 ; - B P E 8 L G 6 6 2 - J : @ C C S 0 @ C * D < B O + ? 9 3 I A C ; L R - S L F 4 C ) 4 < G 4 O B H I > H : > B A M Z X g Z T _ ] L g h ^ O X Q ] R f O [ K G D F R S ` W G A F : B D : 4 8 . E ( ? G K 6 C F + = H K C R E @ - A = C B B H 7 9 V K F F 4 < D E 5 R M R F < E M E Q = K T ] f 2kXwM-tH #Ic/_U Z2 ~ &l"B{{
'<<N37J?rF {G5 l r S f j e V g V ^ { a P [ a b q V X d g m z +Hc (+5yG } e f R G X = J 2 H = H H < < E 6 D ; Q F H / ? 6 $ 6 / @ ? E > @ 1 3 4 + A - E > 5 = D = , E 1 F 7 9 = D : 7 + 8 6 2 < 1 * 8 , > 0 H = & 8 . 5 : 3 9 F = 2 ; 7 < J / 6 7 9 ' C C . E 1 , 3 . 7 $ / B = B A 6 0 7 D = 5 8 B 8 F D L G ? @ 7 9 ; I D > D : 7 < ? E T O ? ( I 0 ; A D B 6 - / M E - < G L > 6 ( C U A > A D N G U Q P G S N f m m -i)gr[B(Z ~ t b ^ i W U P V J E S < 1 N = V B / C L S G 8 Q = Y L Z Q U K L P R m 7 ; < F O ] F = @ Q N Z P H P V W T @ O > M 8 j f p m s >LK- ` a Q ^ M [ C L L > F F J Q ; D O H @ S 3 ? 3 E A 9 : L P L I E H 5 Q E I H E A O Q U D 3 X T U @ M L L U K W : R A ; L P I ? L R B B R A J L F R O O D R L Q Y Z T P _ e d d N Qtu^;
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2a3cb386e39c4ea1932e675727ee1a94Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-23_18-03_0765 3130Seq 12503 12503_Costion_K1315_matK_F jmadera 96-Well K1315_H7KB_3730_POP7_BDTv3.mobSeqPop7z N N O R X b n y $)*)&" #8Ne}hR>* t g [ Q K J L O S U V W X \ b j t ">Xq{ulbWQR[k :VonO*a8 ~ { v r o m j h d ` ] [ Z [ \ ^ ^ ^ ] ] ] _ a c c b _ Z V S R S X ] c h l m m k i h f e c ` [ V R N L L M R Z h | J(UyoV; zbJ3 $hp i5nD i Q A 5 . * ) * - / 1 0 - ) # $ ( ) ) % !
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KB 1.4.1.82018-05-23 19:34:39 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +- VZ DATA
+- VZ ;% DATA +- VZ DATA +- VZ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~
LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS >; P1AM t ` P1RL t Z P1WD t g P2AM t u P2BA t t | P2RL t n PBAS t t A PBAS t t >n PCON t t V PCON t t S& PDMF PDMF >R PLOC t L> PLOC t EV PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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SMLt SMPL SPAC AYc SPAC >K SPAC AYc SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH >i phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +- VZ DATA
+- VZ ;% DATA +- VZ DATA +- VZ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~
LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS >; P1AM t ` P1RL t Z P1WD t g P2AM t u P2BA t t | P2RL t n PBAS t t A PBAS t t >n PCON t t V PCON t t S& PDMF PDMF >R PLOC t L> PLOC t EV PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% >3 SCAN 3 SMED
SMLt SMPL SPAC AYc SPAC >K SPAC AYc SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH >i phDY big phQL c phTR phTR V"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'[A%tu]fH~:$z=%rWM\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUOh'cx+.]gv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]Cp3sV3zg\U9Kzq.`sb?%H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;S AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT ` ` B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +- VZ DATA
+- VZ ;% DATA +- VZ DATA +- VZ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 1 LAST ~ ~
LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS >; P1AM t ` P1RL t Z P1WD t g P2AM t u P2BA t t | P2RL t n PBAS t t A PBAS t t >n PCON t t V PCON t t S& PDMF PDMF >R PLOC t L> PLOC t EV PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% >3 SCAN 3 SMED
SMLt SMPL SPAC AYc SPAC >K SPAC AYc SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H7 Tmpr < User phAR phCH >i phDY big phQL c phTR phTR