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m m ~ r u q o h U Y ] = C M I L @ S H N S R 6 W @ K O Q g ^ j r w ~ f d c p z n z ) r f S k Z c ` ` W T R T U K O J I R P G D D Q W J N [ \ K U _ W m ] S ] Z ] f ^ j e c \ d a \ H ] M q v { b j L \ G W Q V ` _ F R Q R N R H E Q N A D C E E = > Q H V k b n ~ i { n w c | &+ ~ n f l [ K J N \ g M T [ _ M Z h ` c g f g \ x ] o | o | a o o Y s _ d t q j q ` W Z C b Y ] M I N ^ M ] Q \ N Q = J > O C P N I Z O K F C O M L [ Y Y P S b Y _ [ f T N ? < J B 9 9 C 6 4 : < L @ H C R ; P b c { ~ } p h T E F O T Z = D : Q 4 O C S P D U : N 2 5 - 7 K E N < I ? L J W H f d a k c [ i \ y i s j T M I U T C = J ? : E ? = M B L R D F I Y F M W S A ^ d q y X ] = J D D M D 5 < 8 > D 5 4 7 Y 9 B E V i _ _ \ v ~ { x ] k d @ Y R O 8 C N I = A Y E A 4 A 5 > K @ C > G F ; N G : > I A 5 0 ( 3 5 / = > 4 3 0 < , - E > / 6 # ; < = , / C / 2 4 * 6 " 1 9 6 . A ? 0 @ 7 7 C 0 8 / : 5 < O 2 5 2 / . % % 5 " # ' 7 - 4 6 = 2 9 , > = ? 4 = ' ' 6 8 7 F 4 9 ? ; + / 2 - 0 3 2 1 9 2 ; < 4 6 E E K O ] L T Z T g } h b [ @ C I N 9 J A 2 B R ; M T M f e U b i i ^ k y f j j i _ S F I L M N D 0 G 5 G 7 * 3 = % ; . 8 3 6 0 3 : , : 3 = 3 8 ? F Q . 8 @ P ? = 7 > = 6 . % 6 ) % 5 = > + > 8 J / < = \ [ c S ` Y a g m n f Z W W O A E 8 6 B 0 8 D > < 2 B 3 ; 5 G ? G H > C E $ @ C 5 A 7 - / . 0 + 1 ; F ' + : 7 6 M B P V j L V Q U n s x u a ^ b N M O > : L 2 7 . % I 7 ; ( C ? 8 0 5 = 8 4 , : 0 8 + 1 0 ( ; < 0 # 9 : : 9 5 % 6 : 5 9 < @ G . 4 G 3 ( @ = O 5 > L B < T E F Z W H l d g s v w a Z 6 J G @ F R I ? = P ; ) 5 $ - 7 H # + $ 2 ) 4 8 > 5 ( 2 & ) 5 ? - = 8 5 B 5 6 H P 5 J I < / 7 : B > + + 7 1 8 : < 0 5 " - B $ ! : C 8 . / * > ) ' 4 4 - ! ) < / : 6 > > 3 I / ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: df18ddb7e730404988026767145c0cfdSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-23_18-03_0765 3130Seq 12503 12503_Costion_K1315_matK_F jmadera 96-Well K1315_H11KB_3730_POP7_BDTv3.mobSeqPop7z
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[h V5 Av6OO%N~4 P(GACATATAGGGCTCAAAGGACCAAACCACACAGCAACGGGGAGAGATATACACACCCCCTTTACGGTTCCTACACAAACTTTAGGGAACCACCCTTTCATTGGGGAAAGAGGGCATACTTTTCCTACGCATTACGAAGGATACTCCCTCATCGAGTGTGGAAGTAGAACTATTCTCATCGCTACACCGACTGCCTATGCCGTTAACCCCTCACCTTCATGTATCTTATAATACTCCCCCTCCTATGGTCAAGAATGTACGAAAAACGTAGGCCATTACAGTCATTCCCCCTACTCACTCTATACCTTGTCTACTCTTACATTATGAGGTTCTAAGGTAATGCTCCTAGCTCTACTCTATAACTATATATGTTTATAGCGTCTCATATCCGTATCCGCTAGCTATAGGAAGCCTATCACACAATCGTGCAGTGAGATTCCCCCAGTTTCCCAAGAGATGTCGCGAAGGGTATACACTGTTGGGATTTGAAAGACAACTGACTCTAAGCACCTGAAGCCATCAATCTCGTCACGGCAAAGTCGCGGAGGGGGAAGTCACGTGTTGGGGCACGCCGGGAAAATGTATAATTGAACTAGTCCTTCGCTCACTTGTCGTCACCGATATAAGAGCGGGCTCGAGTGTGCCTATGCTTTCCCTCCGGTGTGCGAGACACGATGTGATGAGGTTGTATAGGCTCTACTCTATTATTCATGAAGCATATAATAAGTACAGCGTGTGCTTCGTGATACACCTCGTCTCAGGAAGTTTGTCGGCCGGCTTGGGGTTATACTCGAGATACACATAAGCGCAATACCAGGCAGCGTAACTGAGATGGTGGAGGCTAGACCGAGACCTCGAGAGGCAGCGCCTTGTTCCGCATATCGACTCTTCATAACACTATTTGTAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-23 19:35:12 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ,| X DATA
,| X = DATA ,| X DATA ,| X DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ 8 LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS H P1AM lU P1RL e? P1WD sk P2AM P2BA P2RL z PBAS Lr PBAS H PCON a PCON ^) PDMF PDMF H PLOC W PLOC O PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% H SCAN 3 SMED
SMLt SMPL
SPAC A^ SPAC H SPAC A^ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH H phDY big phQL c phTR phTR % This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ,| X DATA
,| X = DATA ,| X DATA ,| X DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ 8 LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS H P1AM lU P1RL e? P1WD sk P2AM P2BA P2RL z PBAS Lr PBAS H PCON a PCON ^) PDMF PDMF H PLOC W PLOC O PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% H SCAN 3 SMED
SMLt SMPL
SPAC A^ SPAC H SPAC A^ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH H phDY big phQL c phTR phTR vTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3NW'B9*!$>]]c_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl69e >.w#9byH6Rm Tl4oml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~f*Xs\\&N6|MR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4[42g|5PxKoJ-[CW?R5Ch2d'jw~|>J3zi^{g5 AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b / B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ,| X DATA
,| X = DATA ,| X DATA ,| X DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ 8 LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS H P1AM lU P1RL e? P1WD sk P2AM P2BA P2RL z PBAS Lr PBAS H PCON a PCON ^) PDMF PDMF H PLOC W PLOC O PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT !( RUNT 4 RUNT Rate RunN " " S/N% H SCAN 3 SMED
SMLt SMPL
SPAC A^ SPAC H SPAC A^ SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH H phDY big phQL c phTR phTR