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"&' ( $        ! $%%               !  $,   #'.3. +'     " #         *!1+7 '!7.30>81802($$!+ $), #  !      % )!!"*/'":*7*>5!%(,%3.6J3HF12/'984@<8RD9L1DHA?K?D:B18A!-"7'%%,3'?;/# $##)$%4*4#+7!#/:D3@>3?K4<>CAUDWDNHJ$6(;)>/B4K6/&.&%1#%+!0.-"&%% !            %'.#%- $'")%/0*'431*9%*)%!'-.8)*$)& !$!24=;.567,'+!7@.?93)/" %2%)!'   #                #$ ,"% "* $"$$###3"(1)044)*&!-#&, '*&*0%+%#                      ! $"!              %#+ #  ! 4"0 ,!"0$(,$ (%2'/ +4*5$&#%!#!       #"##          '      $#,##1".!! $$!.*              %%       " "/'1$                             &"'!! !        &"$!        %  ( !             #   " & 0 +!!    $           &                   !  * '#061&)  )5 ) ! !                  "                                                                                                                                                            ,,@II^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR#%'&&'%$$""   6Z[[RRRttt(((222==<GGGRQQ[ZZdddmmmvv{~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d19eb66b7a574ed293c2b355825dac5fSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_03-26_07443130Seq1248212482_Costion_K1322_matK_Rjmadera96-Well K1322_H9_RKB_3730_POP7_BDTv3.mobSeqPop7z         !""""""$&)+,,,+,-/2579;>BGLRYbo2e,~/9J=uh"vYAsJ+qR-dB&   &M9Sfs}~q^E) gF( )4>HS`myzoaRA0    "$&&%#! "&-4<DJLKF?6,$                          !!"!    !!!!!"$'+.13332359>DJPUZ`hs <s;)Kf@Ap!T|>;^"7:O  ( t  ;y`#W&2m8PZd)w  b"^ /BA)u&Bo\NC<89<@DHLQYdqvfWI>60+(&&'+3@Rk*Onv@ q[J=3,&" "(2@Sk y[B/!                              !"#"""#%(*,-----,,,,,-.026:@IVjZiLE8Pv=i'iG,   %5I`z"04.5~!M+'BlHz &<NYYN4 GIQ)&U! i9 jYNE?<;=BIS`n}|m^QG?941.,*))*+-05=IZp  x\E4(!!)5DVknU>+                                            '/:GWj +F\lstpg[N@3)!!'1:BEC:,{pga^\[ZYXWWWWWURMHD@>>>>??@BEHKLKIFC@><:86433467752-(# *7EUdq{yn_O?1%           sb@$tAAGZA BKB.bcpKB_3730_POP7_BDTv3.mobtermCCGCCATGACGACGCCCTCGTATTCGTGGCCGTCGGTGTAGGGGTTTGTCCCCTTCTTAAAATAACGAACTGATAAGGCGCGGGGGTTTTTTAGAAGCCTATCGTAATATTTCTTTTTACGTGGCCTAGAGGGGAAGGTGGGAGGTTGTTTCAATTTATTAGAGTTTTTATAAGGTGTGTTTCCCTTTGGGCGGGCCCGTGATGTTTCACCCCCTTGAAGCCCCACAAGGGAAGGAAAGCTGAAAATTGTTTAAATCTTTTGTCGGGGCCGCCATGACGACGCCCTCGTATTCGTGGCCGTCGGTGTAGGGGTTTGTCCCCTTCTTAAAATAACGAACTGATAAGGCGCGGGGGTTTTTTAGAAGCCTATCGTAATATTTCTTTTTACGTGGCCTAGAGGGGAAGGTGGGAGGTTGTTTCAATTTATTAGAGTTTTTATAAGGTGTGTTTCCCTTTGGGCGGGCCCGTGATGTTTCACCCCCTTGAAGCCCCACAAGGGAAGGAAAGCTGAAAATTGTTTAAATCTTTTGTCGGGG2HZlx"+;N^t{ #',7Qn0Uq}(2CJZgw #6GVfs (3?L`t +6CV`o}+?KT`kv   . 7 @ K W a n    + 8 G R ` m z   ) : C P Y d r   ) 3 ; H R a j ~   % / 9 C O Y d p y  '2;MYix2HZlx"+;N^t{ #',7Qn0Uq}(2CJZgw #6GVfs (3?L`t +6CV`o}+?KT`kv   . 7 @ K W a n    + 8 G R ` m z   ) : C P Y d r   ) 3 ; H R a j ~   % / 9 C O Y d p y  '2;MYix"                                                             "                                                             9"/#bP<%<6Q9Z-HN8<WG&(PX]6RHY(D&P=C(ZHFYM-VGKI:C`JC@E9S'D_`6BC"U*+.?!$4>!R#A,NJ>"T!W )B 1ZRXU@A'K>Z \:\'(]M:*+<L*V[T6L-2X7K;PX= B*1 @<@93;I< ;;U6 H\`[9#4J3c&T^`JYD,HEI@ZUKVY# L.[[2ab,=S6M1$>CC$O CX7"/-R\ZVP 0 GtNW3 T* 'D {c 8O 2)"J h%y53\*P8fN[> 7yR?`*dhN,k]!}Fr5<6 IArpL0HeRoB|S q@KY)  +]b:K=41wF?]r| #L.jbW0*pvg,pH~4%%)v>@am.a$Kk7X%I?^ZEP,Yz Y 7D  w 8*8-E+ "N7U(  A- & 7&I5p1^Og=5,JEQ 9vB?i8EDw#vKLB8E!UJzII[mKV(y@h& 4 I,bib jW,U6>Me~s4,Ub+2)o >_E2]f@&HRf] eCJa+w^/`mp#Jfr* - L^If`VNLIQ#a jZ U`1&k=>Q>Qc.n]zfL[&,1JbWb/j~n=#npw` l>eiFl/oKdSEPKE/(|2`adgYkU+FzOpb}\kn0-4M4Xl-Z}=%]r*&M7)-$ IqFUow'/>@]@. / !Ix5O} jGAAAATAAGGATAAATAAAAATAAAAAAAAGTGGTTGTGGTAAAACGTAAGAAAAGGGGCTTATTAAGTGGAGATCTCGCGAACCTCCAAAGGAGGCGACGGGTGCCATACCTGCCCAGTAGACTTATTGTAAAGTATGACCGCCGGACACGTTCAGTAGGAGTACCCCTAGGAAGTATAACTATAAGTTCTCCCGGGCGAGGTAGATCGTAAGAGTGTAGAATCATTTTTGGAAGGTTGGCGTGGAGAGGATTTCTAACCCGGAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 04:58:01 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSBufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA #GDATA #G,CDATA #GsDATA #GDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~<LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS%P1AM  P1RL  P1WD P2AM P2BA  0P2RL PBAS  dPBAS  XPCON   PCON   PDMFPDMF<PLOC PLOC pPSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%SCAN3SMED SMLtSMPL zSPACMSPAC5SPACMSVER4.0SVER SVER,,vScal@Scan3TUBEH9Tmpr<UserphARphCHSphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSBufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA #GDATA #G,CDATA #GsDATA #GDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab'FVoc'FWO_GATCFeat..&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~<LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS%P1AM  P1RL  P1WD P2AM P2BA  0P2RL PBAS  dPBAS  XPCON   PCON   PDMFPDMF<PLOC PLOC pPSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%SCAN3SMED SMLtSMPL zSPACMSPAC5SPACMSVER4.0SVER SVER,,vScal@Scan3TUBEH9Tmpr<UserphARphCHSphDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+@O5+ hl~9;)jM=(ghpMr>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fv(\PjYW1'r>3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#