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"  $, "% "       %"     '$,$+"*  %    ".'"     ! ##            $   %+ &+,'" (      %%    -3.0''.&'$-/#!4(                     # $              *           %$52+" (%')'                                           #,                                                              !$                                        ,,@II^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR#%'&&'%$$""   6Z[[RRRttt(((222==<GGGRQQ[ZZdddmmmvv{~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 01d5e11290024f349ca13f5026c1c413Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_03-26_07443130Seq1248212482_Costion_K1322_matK_Rjmadera96-Well K1322_D8_RKB_3730_POP7_BDTv3.mobSeqPop7z}]>1Ex W =yU*  : L7zS8 [ "$'Y*x-036E8;s>@rBDFGMHkIsJdK4KLXLM/MNNuNNFMLK]IHFCA?> <;9`7_427/e,*_(T&$"!A<)3@gC}[  g 1 -a`z_ q @ 5 > JKDEi@ "B$L&(')L*+,-./..,*{(%#X!6( Q:"'+04m8E;_=>?U>=;w8z405+0% A ~AgTsvh@$'@[lmbVTckN3dMHR`eX9 {{ 0c`C1,3=B7=~$R*kr2J  :yGeA0KZ]XST^o{vWph|T,!9lzg\e?cl_G+ *?XlshI}|~gVWklSC:2' #Cl-M`d\K7% U/'LD}0`uc'a\, 7unbboy_ev<  2[ g/  !]hW&uM9:JXWC&!Z0 ]M LV fJY;]% 2CNQPONOLC3"4^/,Z5$,Irq]UUYYRC0  1BJIB8/)" +:;.4`}qZ< ,n#vkR4gF*5]Nq!m, 2HRL9$+BQO: =~n-JxO-&5?A?=BQg~~cB"  >\r|{vuxcQB738G\pyrZ7(BU`flszzoY;    2lx]YioB *@R^m5eve6~O);]rqZ7#'# 8k #_L`V)}D$$C|(|[o-z|.fE,  # (47.!6HMA* 7\ j2m@<MZR5#roAdQ 0xZroB? 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M ^ i ~    & 3 > O ] q  # / 9 J Z d n   ( 2 E Q [ e r }   . 6 F V ` j ~  "-9CNYao| (6BSZgr|&-8DQbnz$2:GVcr(6@MX_o}"4@Scs~ .:LV`n                                                                                                                                                                                  YXd@O9J7Ea0B,I8(*(E PD>?*1 >]=&,QF%1/[< P1?b=+]T;&G/#=&D6#D9QMDVLWB%N"[E" 7E>]"Y6)Q9[aOc*=a4X #JI#W]B  ))$<&_) DK$:7M8HE)*H 9,0<O#6%%O>6\[d0N$^?&OQ6T^HGM?@* [c358@ 0C, 9:O, M_0SY1#6V.#32!aDS 5:T=A>%%:G 'RB$+K bN=#:$J%8D^K d?Q HLD?T3IN'1 :Y_AE/@_"OI3M6<1<cB&>7:2(N-ZVY=[4V,K+UM)V5L<J,24ABU E+ 0K  K  7dR'<f\:| IpAz B3ys&)kkRlDq%!Oa1A`)~ 3)"<3]tiGVH7/ kN ^KOUrNv_jPL[nh$O0PNP(qloLypUv0q <JD2 x@\vm<RFO O\3>G%V5BczSaD> $yFOg =OTj'=}L<dGJA\ mxuxH05\^;(1QY-O$jb/T2Nj7&"5$:S7EB/7[><F  8 !c%V .=I.J*3%%9J[   $J@* s2, &2->sqpI? p"uVL@IEBPAn* BX=%kj&a@l #(zTVQ([g='q8R 7 SN~d`#/$"BEz/55,#M=S7O;pGdO04l,1DYR^Hi<]w7W6+Ro|kS 8Sk@QJ6;wS6^[CA`i,2F,(4{'Y.ds'F"}d.tv'XAig4{hV:$V]TE$l'N?2>8;7 R(Yq:d[kLrw_xU?dw)UG51[F,aZC}9! &&[3*@*bF&l~7)jL({T<pW \o'^f`6\mC,r%^ v88:4SLxs|I%> /Oz|*r]~M_\8-T&(&G,QC/jK5z",&@5^GGAGAAAAAAGGAGTTATAAATTAGCGGATGAATGGGAGAGTGTCCCCTTTACCGAACGACGCCTCTTATCCCCTGTAGTAACCCGACCCCCCCCCTAAAAAGGGAACGGCGAAATAAGCCACGCTCTGGGGATACACAGGGTCATACAAGGTGGACCCTCCTTAGGGGTATAAGAGGGAGGTTACCCTATTAGGAGTTTTTAAGAGCAGTGTTTTTGGTCGATGGAGAAAAAACGCATACAGAAATATTAGAAAGGTGATGACGCCTTCCCTCAGCGACGCATTCTGTGTATCAACTACTAACGGATGTCTCCTCTTCCCCCTGGGAGTTCCTTCCAACCATATCCTCTTCCTTAATACAGTGAAGATGGTGAAGACTGACGGTTAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 04:57:54 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS(BufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA #|FDATA #|F+DATA #|FrDATA #|FDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AM P1RL  P1WD P2AM P2BAP2RL PBASEPBASPCON cPCON PDMFPDMFPLOC PLOC PSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%SCAN3SMED SMLtSMPL zSPACMSPACSPACMSVER4.0SVER SVER,,vScal@Scan3TUBED8Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTS(BufT#F4CTIDCTNM CTOw$CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#F^DATA#FDATA#FDATA#F0DATA #|FDATA #|F+DATA #|FrDATA #|FDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab-FVoc-qFWO_GATCFeat..-CGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE:LAST~~LIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOISP1AM P1RL  P1WD P2AM P2BAP2RL PBASEPBASPCON cPCON PDMFPDMFPLOC PLOC PSZE`PTYP,PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 7RUNT 83RUNT 0"RUNT 7 Rate RunN""S/N%SCAN3SMED SMLtSMPL zSPACMSPACSPACMSVER4.0SVER SVER,,vScal@Scan3TUBED8Tmpr<UserphARphCHphDYbigphQLcphTRphTRc:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4O6TSN\4RkJO]Qgg9p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=#Lws6FCdh0"o8:"]R_s_be1}1"/"E.Om,P>bH6X12g[jWcgiQF4Np3`4 x'gJx%*{#ODYnWZjdO{7vMjL3ro{`N%7`h?I8rBArIUwch[}k@_a'>yj6m}P,PHq S]*Cy KJFU'.Q1db{wMzR9v!w2l89+uTE#!#hmmfc4hACTT3T&^%FyPC,61h67{=Y`gI)_|_n?NhN{CwN1:^MU9GZsMCTbRrJHuqAGc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`"/OCf7uihukWy4=?6vtfQgsk>H~pL"A~oTq}}89uLgQwCx_R,QJb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0