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(                                                                                   P,,,III^^^{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRI!$%%%$$$#!   9[[RRRttt'((222==<GGGQQQ[ZZdddmmmvuv~}}<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 55b82a9efc5846868a84bafedb220a18Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_01-55_07433130Seq1248112481_Costion_K1322_matK_Fjmadera96-WellK1322_E5KB_3730_POP7_BDTv3.mobSeqPop7z#"J Dnvs[k$ 3 a aU"!$(<+/3F6: =sAEISMR+V`Z=]`bdfaghiTiiig`dap]Y?TPLHDAf=:51E,($w"!!J""""$ !"#$p$%%2%D% $O"! :Ec!!JzQ j i p t M ?MH&?H / P ( z    = V m  o?p!C&-+4|@DrigFsu) SV (D'BxW?>Ogx}y{&;!x DP!3$Y 9>#.lE)S$Rt{e@/ELB+   "MtqF<-B1B !i0+V.O*UzZ-Jo@,_)g/h?[1+9A@8+;}@U}XYcR+<\op]>  %-*#)& #4DTcpz|s_B# #8DD8' -=D?/  ! %($ !&(1Jz DW83,bm;*Ohojiui3   %))#+<B;- 2V3w N~#V<?  cQ q)SO!-i]`mz|oR. 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:.L,?W&/I15 P OQ"?&VSYC W;*YMJ8 &<-(L$ B"QT]EMX !7.ILND/\]M-#%S5@5)H,d/3=13T'T0$ +1JP,4\.1MJD(2J8CN(a%H3&L%#,^D#?4( E^Sa/?(G D6R`-7&NH;))LC];,W).XF#;A)1V7I$;W;P))<, );)H;P; lF)Of8b{pK.#Kq:uyl`g"}Y$cE q+?d'w+6{nQld{CT^V,ypR]ISE#C'b}D$~MTg_'Q NBT"L^p:&u8xKl0N `#{&ypm_8Ur-:.M:+@{qCD 3 h `ww%%K :xZY~5 6IdTp/i{qm M 905/w `S_&j+V%owySbn"/$i=bh D N?w   )Z  `1F$3 #'8/   "(E [1qZ@?1; 9o $*!+%] 1  37,S3fyp;8~sQ((4m%IT9hQoB5,^= _Sp7O[t0r{aus1s<C\_0Hd/K?P:58 yL.)K6,PcE,h}xMKF/:J^ :*1DhGbpN2gy i z6pJH(s:woIdjM V .fp -1qcl(zUrfZGIUAC<VGETX8d(p(H05ykZGqo}\,!OS * -_=_XDbTx~0*hNxT3/LfVaSRx*K| Zp'X30|IV^Uz1<H>YR_9#K!smiypp6M;cT``F9V'icVnN5RZdWVHz3&OJ6tA9j|I+ z2$- / IFLY}|N\S"2`0E:e*ssq<nJ+ @)0:n:UzD]4u,XxIsI#HiC;WGGGGTTATTTGTTAAATTCACTAGTAAGTTGAGTAAGGTACTCGGTCTATGACTTCTCCCAATAAGGTTTCCCCCTGCATCAAAGAGAATTTTCCCTACAGTATAGGGGGCATCCTAAAGTGGCTGAGTAAGGACGGATCTTTTACCGGGCGCGATTTCTCCATTCCGTTGGGCGGGGTGGCACGTCCTTTAATTTACTTTGGGAAAAAGGGTAGAGCTCGGCGTCTCATGAATGTGATATACACCTAGTTCGAGTAAGTAACCTTACCCGCCCTCTCCACAACTTATGGCGCACGAGGGCTACGCGCGTATCCTCAAACCTCCTATGTGAGGAGAGATGCCGTCCTACGAAACACTCAAGCCCGTGTATCGGTCCCAACCACCTCCGCCTCTACTTGAAAGGTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 03:26:56 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #iFDATA #iF+DATA #iFryDATA #iFKDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~gLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS%P1AM**P1RL* P1WD*TP2AM*P2BAP2RL*~PBASPBASXPCON kPCON PDMFPDMF<PLOC*PLOC*PSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPAC5SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE5Tmpr<UserphARphCHSphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #iFDATA #iF+DATA #iFryDATA #iFKDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab.JFVoc.9FWO_GATCFeat... GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~gLIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS%P1AM**P1RL* P1WD*TP2AM*P2BAP2RL*~PBASPBASXPCON kPCON PDMFPDMF<PLOC*PLOC*PSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPL SPACMSPAC5SPACMSVER4.0SVER SVER,,Scal@Scan3TUBEE5Tmpr<UserphARphCHSphDYbigphQLcphTRphTR<8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0HVRJxG57'9\47.<<_Z=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#