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P,,,III^^^{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRI!$%%%$$$#!   9[[RRRttt'((222==<GGGQQQ[ZZdddmmmvuv~}}<<<<<<<<<<<<<<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 905e38cdc9ee4feda618296dc0c147bfSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-19_01-55_07433130Seq1248112481_Costion_K1322_matK_Fjmadera96-WellK1322_B2KB_3730_POP7_BDTv3.mobSeqPop7zqD:*'Q,&ZjI]Iy!NWOJy*NTWzQ5 x#36 . d.+KbJGUl 6 -IeBzwm=`|* B F G  \  V  D } M T  \  g 5  { j e G  h & g U , 9 11&;)ovf.0~\ Jn %B1#&(,-9Rji;l `.RZNa%{W!?U8^]5E1-b)W+&CqU!,.=Ps_gjs@"u!4=h/HV Lxg: R ]+nl7vR.Jl.PJ>n2Nipqbm` [Jn=p/7{oWg8~9;?e\5,*ln)a< PBGx}I /76," tq &.1#  !   ;jk;  !A]jcK+ #Rh9!/0 \1zO+ :k!Fobp5  Ca)6a]/ 1??4)&+16?]sVN^\CSOiAX(?j-i>LIg4K Knf?ToE* S@aAJDkH {# LxA&p?PLK[kE,'? b JacfJTlDz Q w\TU?"q< ^RRb ,  L/tO'g/!Kj;  ,M!\| e= "*";qd&`]D(eK`mY}RSC +Uc** yKP)wOOq' SGM,:;4jB2% 2#"P 0:S  ~><NIQ!{[Loj3W z.&b %- mIe ?g[1 7LUM7#TV.8Vnxsf\_q}KU|A?jclAub;!p$m[S1.d^0  9~AUQ;G !/0# >:#9F *'zMGDsq/q':f;ZSbWH   (T{h=  EcO,je#X6[Cqg' A|  /{Lmjo)eT.W0:n{\) o^:?]Dd?bk0-LK_ls,AmEp{,>9 .,NOZ sPN?9\c e2XWYX+.t`QfSNPSTSOH>2*)3COJ.NiZ%gJ:*6e[.  $=Y{3zZ7+_!R =ksM!L~A  ?W^P5 $iW11 (*% A0Sh"Ub9#(  "^$v&*aY:1CnV!  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(35- .;;/):@7% &5CMQL=(/?D=- 8\|w_RZwO$'-(  "  !%#  'Jj{ycC!;\qq[8 15+)=ID1   $JofA$3[a7!5EJB.  093#  J~~L#0]`0   !=RWJ4%)DpbI4    '12)  &;HH<' !$$#$&($  A]jaH) 4EKD2 0H_p~~c@  %,18G]tz^4 *iN "4=8'Ck~b: ).) +CSUG/ !>V_V>    5fn8 )ur(/B@,    *0,  1BF:$ @w2 KA  *9>915P y/ 1ac3(Z]21Pgqph^UKA848DTabT9:NSF-"A[f`I+*7:4'";Xr|hI' !3@I%7J/@sA =A>GAKB.bcpKB_3730_POP7_BDTv3.mobtermTTATATGCCCGGGGTCTGGGGGGGTCCTCAATCGTTTTGTGAGTTAGTAAAACAAACACCCTACCCCGTCCCTGGGGGCAGATGGTTACATCTTCGTTGTGGGGAAGGGAGGCTCCTTCTTTGGTTATAAGAGTCTTCCTCCACGAGTATTGAAGTGGGAACTTTATATGCCCGGGGTCTGGGGGGGTCCTCAATCGTTTTGTGAGTTAGTAAAACAAACACCCTACCCCGTCCCTGGGGGCAGATGGTTACATCTTCGTTGTGGGGAAGGGAGGCTCCTTCTTTGGTTATAAGAGTCTTCCTCCACGAGTATTGAAGTGGGAACT")2=FP[fr*6CMZky )3BIS_ju $,6BS^jt (6FS^ks ".<DP[fq~$3>HV`kt-:IVdlw")2=FP[fr*6CMZky )3BIS_ju $,6BS^jt (6FS^ks ".<DP[fq~$3>HV`kt-:IVdlw       &    "                                  &    "                           *3(3Q>MVL8<`>51M"-8":9U50cNYSU"D91U5$XK++)GN"VCQL M:B E.=:0'\X6@S64KBY'* ^B&)VK;1.P-DJ.G8P"3 I;\`&ZB@6=/M1@PaP^QJS5+ CW g H I e   o ]  % F-[3aA)1 K#,A{|V ^M~#]uI/JS&Rxu z8Z]8L5f~cQ.O/,Q/  g8LhTG,7VIh'bcwlX#nvaM>a_FyX5[VW<0u]0Pl_51 !f O  XUsV9+* FAD8C'[gNpl!^M*%d!,$d9,d2uupiVwPf<Vd1s8363(L2H,2U0,XFE U r  T8+!M\_CN)=/*$G3vS/&.UE%@bxy!<bX=?f0RhUa_SK?`wG`n~Pu[yCXW_b~ ^;... LAATATATAAATTAAAGGAAAAAAAGTTCACGCTTAACATGTTCGAGAAGGTTGGTGTCGTACCGGAACCGTACCTCCCTTAGGATGGGGGCTCCGACCAGACTAGGAAATACGCTTCCACGACTGGTGTGAGAGTGCTTCAGGAAGTGGGGATGTGAAAGGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-19 03:26:30 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS RBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #TFDATA #TF+}DATA #TFr%DATA #TFDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS}P1AMFP1RLFP1WDFTP2AMF P2BA &P2RLFPBASSPBASPCON%PCONPDMFPDMFPLOCF<PLOCFPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%uSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER  GSVER,,Scal@Scan3TUBEB2Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS RBufT(P\CTID+CTNM1CTOwLCTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA(P^DATA(PDATA(PDATA(PNDATA #TFDATA #TF+}DATA #TFr%DATA #TFDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..mGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS}P1AMFP1RLFP1WDFTP2AMF P2BA &P2RLFPBASSPBASPCON%PCONPDMFPDMFPLOCF<PLOCFPSZE`PTYPTPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 70RUNT  RUNT 5RUNT  Rate RunN""S/N%uSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER  GSVER,,Scal@Scan3TUBEB2Tmpr<UserphARphCHphDYbigphQLcphTRphTReZ>\&bD8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|$q_6U3ybK4ZaHUt`_#3\Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*t> ;.1Mrh3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SK-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]w