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[[[RRRttt (((232===HHHRQQ[[[dddnnmvvv~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5f4cc5f7bb024e04adaa122c2ce2ffaaSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-18_21-23_07403130Seq1247812478_Costion_K1320_matK_Rjmadera96-Well K1320_B12_RKB_3730_POP7_BDTv3.mobSeqPop7z 5c<8o7=WX} " , : u |I@(' k&r" Y :  R H]wc<tq}8])Ochd^_iyjQ;.+1>Ph$-'zj^^m H  s  8 ^ v { o a T F 3  l L@S9eH?BJRUTQONPRSPLIIQ`u=h BnsP)<Uy?}]J@;963/./39AGJKLNS\hvq`PB82.+)(,:UePn!1pwl*{l[J7$ /Kl)k8W"xoNUG0yfP8!7yi_@Gzbc# "$$" !).149@IQUVSPNNR\q8g&^NK{C,Jl)Iezx`A!A { |vT56S4`y}mImC)!,FkxS907Orc6GmGeXh6$7K`vxaM?88=FOW[ZTG7%)12+ #! .?NZcjnok`P;%$BeeJ4! $@`~bC'*=O^ed]SMNYj|{dJ1#/<GPYajrz~wmbXNE<3*# "#$(,143/'%0;CFD>60,-3:BFE>4(  !!  )9EJF;+  -E^tuaOA<BToW. /BORK>.!"-8@DB<2('07971)!"(,//.+(%$%'*.26774/'+Hf~x]>!  6TowdQB=CTnmWLMWgw}ysj_RE;647;?A>6* ,9FR]hoql_M:)  !&% /EYjuxulaWOJFA9/"/Sw_>" .BRZ[XVW[ad_S@*   ,?PYXO>+ !&'"!$# !-9BEB9."#-7<;4)%,/,$+5:92'  =b~Y4 /Mk}cE)   ,;FNQRQPMJF?6+    '++&   6NaidT>& /Hc~rW?,          ,7:6+$*,(              $'&" (.11,&"(,-)"#)*& "'%    "%&$  '*'"!"  !!  ! 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"!        $+0/( !"!                #'(%         $()&    !##"  )374+  !##  &)(# ).,$      "$!      )-+$ #"                                   ""             "',//-(#                                         #&''%"                                                                                                                                                                                     h@߭CAA;:A eKB.bcpKB_3730_POP7_BDTv3.mobtermGCTACTTAGGGAGTCTCGGTATCCGTTTATGGATGATTGATACGTAAATTGATAATCGAGTGATGTACACATACCATTCAGGTGAATCTAGGGGTTTCTTAGAGCGCCTATAACAGCTCTCAAGTATTTGATCGTAAATTAAGCTGCCCTTGGGTGGAAGCTCCCATGGGGGCGTGGTGAAAGAGTCGATATGATACATAAGCTAGAGGTTTTTACGAATTGAGACTACTTAGTGGTAAGAAGGGGTTCCCCAATTGTTTTCCCCCGAGATCGAGGGAAAAATGGGTTTTATAGATATTCACCGACCCGGAGAGCACCATAAAAGAAGAACCGAAGTTCTCGACAGATAAACTCTTTTTCTCAACCAAATGACCCCCCAAACACAGTTGTTTAGTTGTCTCTTATCTTTGGAAGAGGGGCATCCATGCACCGATGCCGCGTGGAAAATGGATGGGCAACCGCAAATCATCATGAACGGGATGTTACCTGATCAAATACTAACGAAATAAGCTACTTAGGGAGTCTCGGTATCCGTTTATGGATGATTGATACGTAAATTGATAATCGAGTGATGTACACATACCATTCAGGTGAATCTAGGGGTTTCTTAGAGCGCCTATAACAGCTCTCAAGTATTTGATCGTAAATTAAGCTGCCCTTGGGTGGAAGCTCCCATGGGGGCGTGGTGAAAGAGTCGATATGATACATAAGCTAGAGGTTTTTACGAATTGAGACTACTTAGTGGTAAGAAGGGGTTCCCCAATTGTTTTCCCCCGAGATCGAGGGAAAAATGGGTTTTATAGATATTCACCGACCCGGAGAGCACCATAAAAGAAGAACCGAAGTTCTCGACAGATAAACTCTTTTTCTCAACCAAATGACCCCCCAAACACAGTTGTTTAGTTGTCTCTTATCTTTGGAAGAGGGGCATCCATGCACCGATGCCGCGTGGAAAATGGATGGGCAACCGCAAATCATCATGAACGGGATGTTACCTGATCAAATACTAACGAAATAA9?NWen|-4CN_kr| +5>KU_kpu|)28ALUlw "+6AMYgt "+:ET\cr| #+9COZdoy&4AR^lv%0;GT]iz  ) 4 @ K W a k {   , : C K X d o |    ) 5 = H S ] h t |   & 2 = N W f s   % , ; E L ^ k u   &0=GP`hq} '9FR^gw #3<GQ_mx *5DQZcmz '0;FWdq />FP_ky '0>N\iw!/AN]iy+4@P`ox1>P]k{ %1>N9?NWen|-4CN_kr| +5>KU_kpu|)28ALUlw "+6AMYgt "+:ET\cr| #+9COZdoy&4AR^lv%0;GT]iz  ) 4 @ K W a k {   , : C K X d o |    ) 5 = H S ] h t |   & 2 = N W f s   % , ; E L ^ k u   &0=GP`hq} '9FR^gw #3<GQ_mx *5DQZcmz '0;FWdq />FP_ky '0>N\iw!/AN]iy+4@P`ox1>P]k{ %1>N   -  +% , >    )                            "        "!"                                                                -  +% , >    )                            "        "!"                                                              N)"Hc>)&PFBJ51cJdPQ *RP@B[]\[/FC 6/`G'G]<UK`H>, 3I,"b)` /< &Y\ 2H 0/\I8NcC'0QB$SW#? Q?X NN#2?cU;Y)`4ZH;@b1$B ?L 0C+B8\89AbbH@7c#c,, 9];GA-A _>@O<5= 1% _ WAW9ZJ@"%/K4;ZY %;&>K D;'c 4:"5b9(VXZI<UIQD=SFSW"O?#a`]<$]"cP' BFBAJb40,L?@D OC5=$.3cJ%M -OJb T2&UQ' aI EE ;cQNF4 5O \.6-GA#_(/ 34 5;OC4+Y]-(D>00$@"]= )P9^&N1 S3"7IE>(+ <bIQ[- OQ#"48 TQ()#*8K!+ 9["$G1O*# WQ8Y T#E(3 04GLa_>4A!(QFMC,.Q R07- > R 9' =KbqwY*U<2yd7H>J }hPJ> % _8 of 6a:) o)5|-p3 >HZ+oP.qkFl3zoV<<:pt'N!; z oc&HKcp z}.vC-|Zxd,EX%lT'*r[^ZJG<lDz SX\R  m"; 2%; ?@[K)_i>DW6+f=.Q*Q{DpH{Vf IdclQo]PD@ W`,~k;PDK,N:W!lbka6A8 H< 6 D8_- vdXS6()O +>=PDexL\q pQ]2[SZDoH&7(d&7 Q?l:6  =! ((a_*J+#7 U [| !",4 38!D][ < , -d5:L0{F1JNnhox 4'| 'C)L R LF#$*4ds'JGYY]#{/eQ]e26-D3Y &Y`?H=pu`{;Y:G8qpzMCG-*JkXVWo8m/ppQJ'DI[_tNetbH a:sAk8d2 ?S4KZBq8\G">o#,$yCTp;F$~>-TET d k(8o'`{8tz%p) sOuyj8e-`WWZYBT mCOc0|Tc?q  ,<q@?l]W8C4xl>E,*8Vi3~LpC.mu8!T=tcp>a3z<:$IPc[U<\~^-pZW  ' < J 9 x? O<x6Wcp=k - TlKad:8I\DK0OSE>wy(CUf~Mw\eXy%Xy:L cshRbC2,~YhGxgF`uW!N8aPA], >P 2QGy  ?+)QZFlSm_'c@ MY!XY<j%C?Kd4;hgs~]% 52o]*&r a!D bxJJKnXhodqA}8`x,4.x%9nAQ06Q H~m+fN31/%i>>Z< F8SIdiQLc$8ve!yEpxZdz!3vYt[~tx D )TGAGAAAGATAGAAAAGCTATAGGTGAATGTAGATTACTTACATAGGGGGAGACCCGCGACACGCATGCATCTAATCCTTAACTTGGAACTAAAGGGGAGTCGTTAGACTATTACCAAAATGCAAGACATTAAAGTTTGGGTAAAAATACACCCCCCTGTAGAGATATTAAAGCAACGATTTATTCTTCGGAATAGGCGGGTGGTACAAGGCGGTTTTGCCCCTTGGCTACCAAAAATGATTTTCTGCTTTACTCCAAAAAGATTACCACGAATTTAGGGGGCCTAAGACTATTGACCCTTTATCTTTCCCCGAACATCATGTTAGCATGGACGCAGGAGTTTGGAGATTTGCTCCACCAGTTCTACGTGTTTATATTTGTGCTGAAGCGAGTCCACCTCTCCTCGCAGCTGGAGCTATGTCTTTATACATTTACTACGTGACGGGGGTATAAACACCAACTCGTGTTCTCCTGCAATTTCCCGCAAACGAACGCATAGTGATTTTCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-18 22:54:49 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS%BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #5FjDATA #5Fj+DATA #5FjqpDATA #5FjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~$FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISLP1AMaP1RLgP1WD[P2AMOP2BA"IP2RLUPBAS|PBASPCON jPCON mPDMF|PDMFcPLOCsPLOCyPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%DSCAN3SMED SMLtSMPL pSPACMSPAC\SPACMSVER4.0SVER %SVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCHzphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS%BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #5FjDATA #5Fj+DATA #5FjqpDATA #5FjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab4)FVoc4FWO_GATCFeat..3GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~$FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISLP1AMaP1RLgP1WD[P2AMOP2BA"IP2RLUPBAS|PBASPCON jPCON mPDMF|PDMFcPLOCsPLOCyPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%DSCAN3SMED SMLtSMPL pSPACMSPAC\SPACMSVER4.0SVER %SVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCHzphDYbigphQLcphTRphTR^pF?jA]RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+@O5+ hv~RA)j*=~QhQ82>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fv^\~:Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfK|b||WOFBo3RKy\rm#ulc*{d&]?#(eaK|`P@R(RlSR5y=Y`gXBY'^n7a;A{%i7#W yZE!e~8WYH|O[nYL.wJiBwH~?p MJM*{B]9[ikI0o6Gzv&1`Zlc*+?_u';fQGKerctp(kA;M4[!f<,hm<7eaAvG9 HtWo0>B|::zWT|O\rwHB*@Y%H_TQFGa^w^8/HAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDTffB<B1PtB1PtBCTS%BufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #5FjDATA #5Fj+DATA #5FjqpDATA #5FjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab4)FVoc4FWO_GATCFeat..3GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE^LAST~~$FLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISLP1AMaP1RLgP1WD[P2AMOP2BA"IP2RLUPBAS|PBASPCON jPCON mPDMF|PDMFcPLOCsPLOCyPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT 5 RUNT ,9RUNT 3$Rate RunN""S/N%DSCAN3SMED SMLtSMPL pSPACMSPAC\SPACMSVER4.0SVER %SVER,,nScal@Scan3TUBEB12Tmpr<UserphARphCHzphDYbigphQLcphTRphTR