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[ [ [ P R R t t t ))(333=>DHHQRR[[[eeennnwww~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1a1e1220bb6b46c78308c159662c97f5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-18_18-21_0738 3130Seq 12477 12477_Costion_K1320_matK_F jmadera 96-Well K1320_H5KB_3730_POP7_BDTv3.mobSeqPop7z + 4 A S h x { o [ K E L ^ v
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Q*t~WO N0^'ACAACTTCTCTTGATAAAACCTGGAATAACGAATACTTAACTCAGGCACAAACACAATACCAAAATGGGAGCCATTTAAAAGACCAATGCACCGGAGAAAGAAACCCTCCCCTTCCTCAATTTAACGGAATCGGCTCCCTGGCTAGTACTAATTGGGACCTGCTTCTACCCCTGACAGGACTTTATATCGGACTCCACATGGGATAGGGGACGTGCCTGGGGCTGCCATAGTCTACCGATGGGGAAGAGGACGAGCTTTAAGGCAACTGACCCCACAAACCTTTTATAATGAATTTCAGAAAAAGCGGAGACTGCGTCGGACTGGTCCTTATTCACCCATAAAACGGTGAATTTTCCGCATATATTGCGTCTATCTTTAACAGGACGCTCCAGCCCCACAAGAAATGCTTCATATCATGTCTACTTAACAATATATTAAGCGGCCGAGCCAGGCGTGACTGCTCTCCGTTGTAAATGAGGAGGATCAGGTTAGATACCGCCCCATACTTGGAGTTAAAGAATCGAGCTGCCCACACACGCGTCGAATAGAAACCTTCAGCTTTATTCCCTCTTCCGTAATGGAGGAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-18 19:52:03 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS l< BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +E V DATA
+E V ;# DATA +E V DATA +E V 7 DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ! LAST ~ ~ j LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS > P1AM M U P1RL M Qd P1WD M Z P2AM M c P2BA M M hf P2RL M _2 PBAS M M AI PBAS M M > PCON M M O PCON M M L PDMF y PDMF > PLOC M H0 PLOC M C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% > SCAN 3 SMED
SMLt SMPL p SPAC M SPAC > SPAC M SVER 4.0 SVER l1 SVER , , n Scal @ Scan 3 TUBE H5 Tmpr < User phAR phCH > phDY big phQL c phTR phTR { This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS l< BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +E V DATA
+E V ;# DATA +E V DATA +E V 7 DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab z FVoc z FWO_ GATC Feat / / zW GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ! LAST ~ ~ j LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS > P1AM M U P1RL M Qd P1WD M Z P2AM M c P2BA M M hf P2RL M _2 PBAS M M AI PBAS M M > PCON M M O PCON M M L PDMF y PDMF > PLOC M H0 PLOC M C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% > SCAN 3 SMED
SMLt SMPL p SPAC M SPAC > SPAC M SVER 4.0 SVER l1 SVER , , n Scal @ Scan 3 TUBE H5 Tmpr < User phAR phCH > phDY big phQL c phTR phTR Py]]~I8e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|L:hNt\~!(oO3'ThHThMuIlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,m &~Mue\,KZI\T(k"T'o)6yHAj}=D8m%(4Ws _ZZ^sSsYiSOpP S!oSRb;6AI8rBA(&jEaC9}RiTK(>V&bJ~FTWYhY8|kb+N4+~[{Hu#e./f<@j2QuOzcI0 Vps5\iDhkkJ/n=dNL?Hr&NBY\IVys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L P1AM M U P1RL M Qd P1WD M Z P2AM M c P2BA M M hf P2RL M _2 PBAS M M AI PBAS M M > PCON M M O PCON M M L PDMF y PDMF > PLOC M H0 PLOC M C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% > SCAN 3 SMED
SMLt SMPL p SPAC M SPAC > SPAC M SVER 4.0 SVER l1 SVER , , n Scal @ Scan 3 TUBE H5 Tmpr < User phAR phCH > phDY big phQL c phTR phTR