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7 z | e ^ m Y t I ` _ o =Hb ' Pxw3]V o m u t f N ] L e v w s a^a="@ y q c y s m j \ _ a c N R K D T K T F P Z Y [ X J E M H , D 1 D C S 8 C V = R K 0 ? " . , 8 L C R E L G ^ K [ \ ` b P M b x V Z i h \ l e g Q y ] e k ` v a T { e n 0gmc * S { | y l j Y S f Z e v !R . Vra" | j ^ m Z m _ W E U 5 T V ? C V E W M C D P 0 ; @ J G E < O B U ^ Z S _ K a ` b z m a ] r _ w c t m Z y j m fq4 q,]- ~ k u h d W ] l V G J b G T H B ? d G Q P U G X ] [ ^ T g T ` W [ v w l ;CN $ [eS[# _ H s W k Y h V A O \ \ @ J O S C C ` T T Y U [ S q q { g b } x T ` T i f g p U o Z W P [ Q ] p T ^ q D]Ajv*rQn& f T ` I \ Q W R J L G B H L $ @ I B 5 @ 2 5 9 2 6 7 1 ( 1 0 + W C K ? E ; A M , ) : . = ? N + / 7 . ? % > Z ( F e 8 4 . 9 < 3 < I 8 A 2 ? : ( + ) * , 0 : A J 5 M 2 ( A I F 7 9 I C K = K ; 2 3 * 0 " # 2 8 > , ' ) " & * ( A # 3 C $ 3 * ' . 6 " ( ( # . 8 4 " B , 4 5 B . ? < 4 ( 0 4 A F 2 ( 7 C > ? 6 L 8 L D F I R 1 - 9 8 D > = J 3 G 5 6 B 4 2 5 % : 3 3 0 / 5 < D ? @ 7 7 > + % 9 ) 0 F B * G 4 C L O R D P F V S K I D + L 1 R = / K ? = V C c k v {F6$+s"LNcP w p g B L S ? B G ! ' * , 3 ( - / 1 A F H ( D 0 J 5 H 7 4 @ : D 0 & 2 ; 7 * : K * , ; 3 - " < 2 0 - = 6 % 2 / , 5 A / # ! C 3 ' - 7 F % : J 6 ' + 5 $ - - ' 2 1 : " . 2 / 3 / 1 C > 6 ; 0 4 2 - 3 A H B W E E S 2 + 0 = 7 J = M ^ d I ^ Y z E#TeOBE | d a ] S c > P L 4 A Q 2 ( @ : 0 ) 0 / D * - ' ' 1 ) 4 2 1 ) + E 2 5 9 N U S 8 * F C / O 9 S N J I / J 6 : @ $ ? H 0 ; = , 6 . < 2 ? A < 6 E 4 A C O = B 6 % I B = B D A X N I Q Q I G C D @ / > 0 H + 0 D 5 0 ' 2 0 8 ? B # 1 - L < . = 1 * : 6 < / : + - 0 # 0 # 4 / 3 ( 2 / 1 A L 8 : J F \ M L _ L M ; : E S - < 7 " ? Y : B K E M Z I @ 9 6 1 : 2 3 @ P ' 8 - , + H 2 * 4 & # % ! ) - = , = ( * 5 8 ) . 1 = N ; K F H K < , ? < . < F < D 4 = 5 F M 3 I D 0 = > < ? I T d | f | mw?a%2^jKs, r a j Z X d Q ` = B = Z e U X - M I m _ G h Z q u k ?+?R
5PNtI:)h894|L u m y _ P j h b G ` C @ A 9 ) C 0 > @ 5 ; : B ( ; J G G N 4 P X 7 6 & < 4 B J > 8 O G F = G O 7 C I ^ T @ O K D Y = M > E ? ? ] * * 4 6 & I " < H * I A E : X C B + D 5 A : ) 3 W a U L F B R G N g ^ T _ W e V Q I ] Y d b T T L T a U H S e g } k Y$I;-C9n7 _ T Y S ] Q R 8 & K G K 7 H B = E 7 ? O E < G T F b I @ K 6 9 7 O N F I D 6 Q ; B C 6 8 U : = G @ ? < A X M 1 ? = = E Q V \ L n d n s x LxZHp_Z9z#Rj9/.Q(x ] n v g | b f i o i i g N#L!2K|/ a s a F Q < Y S G A Q I A Z A 0 [ Q b F i ^ k { u y H<EpnPM2*D( y g G U O @ > 1 B G B . a < T H H Q J H H B D H = Y H 7 C P J B / : 4 J 5 * @ 7 ; 7 2 ( = L > * @ : J 2 F 9 M 7 + S 4 H 3 ( 5 0 - E B ; ? = 6 - ( . 4 ; 3 ? 2 D L > = C J > < 9 @ 9 , 0 5 % D 4 ; E : 2 - G > O : 9 8 X W T T B S O X H e < @ ] H K G M K V ] O [ S P [ G A 4 < N : B M H F D / = . > I ? 6 N < B O 4 A M 2 3 ; 5 ! ) - 2 $ 9 ] P S d 3S"w~M m n \ S I < 4 D " = , O 1 , # @ * ' ! 1 " 4 $ . D 7 < / A L F = 8 I W @ E J I 9 = 5 A H 6 6 N @ N B % , + C * / , & ( . * 1 : . 6 $ . G 1 6 4 < 4 $ . ) 7 7 9 ; ( O D ( @ + G F @ W U 7 : I z ` { x z $uQSD!)E?. ^'I
c' N p [ ^ : A Q C g : 9 M : E B Q \ = : X > 5 J O { b 6(^|Ynw, y T Z C Y @ I ^ B N E , L I I < K L d U Z F O ] z { z\(~7tQH
n r m l ] J e k O a [ F W%\Y{RcPA h t j ` T W U O < I H C 2 R ; ? @ G F 5 ) 6 : ; < A 2 ; J 6 A < 6 # E ; " + > ! < @ / ! 2 2 / 6 > = @ C 7 8 : : R K G @ 3 6 D P 6 R > A G : J f X e b V L W V I a b D J \ l b V L _ i (_ }Q}?TdU& | c f ^ X g t -nM7/^ D dw+Hw~_W`/ l f g b e G = C K 7 N F D C & [ 1 3 4 G 8 . , . . . / $ @ ! > ' 9 3 ? 3 F C 7 2 A A a B Z Q P P I ` c P S W X H Y = ; C ? 4 , H 9 / 7 @ 8 2 . B 3 J 2 I B @ / 6 O L A G H 9 M J > 9 H C 2 ' 3 1 ; & % 9 , . - 4 - 6 # 3 & - = 2 + 2 8 # ! * 2 $ + ( * > 8 1 9 % % > $ 1 2 . - 5 1 > 9 0 6 > 4 % C ? 1 ? B 5 @ B G L B 8 - 0 N 5 3 ? L 2 / $ 9 ' I / B # / ! , > S 2 = ; Q 6 ; = 3 G 7 A A : & = 8 = & 7 5 : > & ' ) * , $ $ & < . 0 ) 8 5 ( $ 6 , * 4 : ) ! - % ! / 2 + / 5 4 * = C 8 / $ ! (
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e441aa2e05f847b18b80f78119286b2eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-18_18-21_0738 3130Seq 12477 12477_Costion_K1320_matK_F jmadera 96-Well K1320_C11KB_3730_POP7_BDTv3.mobSeqPop7z | y v q j b Z R K F C A @ @ @ @ @ ? = : 6 2 . + ) ' & & & ' ( ) ( ' $ ! ! # $ % & ' ( ) * , / 2 4 4 2 / - - 3 = J Y g t 8tChY<,_G#B4cJ=;CSiGx2[X2'@ q ] O E > 9 7 7 9 = B G J J H F D C C D C @ ; 4 - ' $ % ' * , , * ' # ! ! " % ) . 3 9 ? E H J H E B > ; 9 6 2 , ' ! $ 3 K n )bF 2(u.O q K 3 %
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KB 1.4.1.82018-05-18 19:52:57 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS b BufT " D T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D R DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE [ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS =B OfSc r P1AM ` P1RL Y P1WD h P2AM v5 P2BA }M P2RL o PBAS A PBAS =u PCON VI PCON R PDMF PDMF =Y PLOC K PLOC D PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
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RUNT 11 Rate RunN " " S/N% =: SCAN 3 SMED
SMLt SMPL
SPAC A\o SPAC =R SPAC A\o SVER 4.0 SVER W SVER , , Satd ^ Scal @ Scan 3 TUBE C11 Tmpr < User phAR phCH =p phDY big phQL c phTR phTR :ā This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS b BufT " D T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D R DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / } GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE [ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS =B OfSc r P1AM ` P1RL Y P1WD h P2AM v5 P2BA }M P2RL o PBAS A PBAS =u PCON VI PCON R PDMF PDMF =Y PLOC K PLOC D PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% =: SCAN 3 SMED
SMLt SMPL
SPAC A\o SPAC =R SPAC A\o SVER 4.0 SVER W SVER , , Satd ^ Scal @ Scan 3 TUBE C11 Tmpr < User phAR phCH =p phDY big phQL c phTR phTR Op]C$xX+lkfc40-fKbVUg|=a1tirD:Z5A>9^kZdg~WI|Mb;eX}"if32#/P[~W!Fy^q3Th$Y i ^UO ]ddc}?8d)sGi=&MQJ1r]ZvZ~h6dYHa?yh;}S$PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AV^V4?HV;mUCBM[?|$}d& 'iJ4~CubW_]?ZC'T3T&#ONx8LkV}d7?#)io5`N0W`,#Xr_<.Fi1A+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\W\lWQ7M3gptQ>?";!8L[2Rr`qHT^1R=goQS-CDiyX^UM]N=?W.ss$T!D@4ir$Gj~`? MlYXjlnK[Yl`A #pJE,#VuU4zr4v4*A~a/.c{F#nVGW$YD>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#j1DjU5]DtmXY#agsC!.:QI5&.XRz+*}Lq&Wi1Wc]4@3}@U2]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0SbXW,VE+,[Ol% eIe]pBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si! AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b B1Pt B1Pt BCTS b BufT " D T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D DATA " D DATA " D R DATA + V DATA
+ V : DATA + V DATA + V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / } GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE [ LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS =B OfSc r P1AM ` P1RL Y P1WD h P2AM v5 P2BA }M P2RL o PBAS A PBAS =u PCON VI PCON R PDMF PDMF =Y PLOC K PLOC D PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT 3 RUNT +
RUNT 11 Rate RunN " " S/N% =: SCAN 3 SMED
SMLt SMPL
SPAC A\o SPAC =R SPAC A\o SVER 4.0 SVER W SVER , , Satd ^ Scal @ Scan 3 TUBE C11 Tmpr < User phAR phCH =p phDY big phQL c phTR phTR