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"  $                                                                                    $         $                        !!                                                                                                                           $                                                                (,,,III^^^{{{&&&999DDNXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR!$&%%%$##"   [[[RRStt(((333=>=HHHRRR\[\eelnnwww<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d267e707cc174bd7b74d9baafb1ee86dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-18_16-50_07373130Seq1247612476_Costion_K1319_matK_Rjmadera96-Well K1319_A5_RKB_3730_POP7_BDTv3.mobSeqPop7z-XrP,Y=B"\$z7 ; ( s   ' 4 I h ^  a | > y%aUm-XnM*vbPA5,$+:K\m|{lY@"y\C.~|{{+C_ *Hd{Z,oUB2"yj[NB92.,*(&$##%')*+**,18@JT`n}wsojfa][YVSOJHIMU`n|vjb^^agoyiR@8>WZ8}YCCMW^abdfjmmic[TQQT[cjooj_O<)  !',037;AIT`jrvwuqmklr~y]F=Nc`pqB')*Dp]au9BL&=c)  %08<?@@??>>=;71*#!(2<DILOU_o)5;8,jVG;/"9 K>qlXI=3,'#  -BWiyW+ $WK7M^jt.97' {[?'  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'2=HOQME9-$$+5>GNTY_dks|wlb[WVWY\bjry|ypaN;+    (4;<70(" />FE;,-<GJE9)  %)+06@KUZYP@-  #(($  !       %4@D@4% #&#.:CFD<0 -9ADA;2'     %6BD<.4LcszwkZG6(&1;?<2# 1@HH@2! !!    &'$          #/;CGGA8-   #&%              #&%',+$                   #$!  #''#   "#             %&$                     %'$                    (--(       !$"         !!                                                   !')%                             "#"                                                                                                                                           Ax@iA4A@AKB.bcpKB_3730_POP7_BDTv3.mobtermCCTACATTAGTAAAGCAAACTCTTACTGTTTATAATGTGCTAACTGCAATGAATTGGTCGTGGAATTAGATATTAAAAGAAGAAGGTAGATGGTCATTGGATTTGCTAGCTTTGTTGACCGTCTAATAGGGTGATTGTTGAGGATGGATCGCTTTTTAATATAATATGTCATCACACTTTCATGGGGGGAGAATGCACTTCCACCATTGTGAGTGTGTTGCGGCAGTTCCCCTTGGTTGTTCTTCCTCCCAGTGCTCATTCCCTTAATCGATTGCCATCAATGAAAATTCGAAATCCGGGTGATGATTGATCCGATTACTACGTCTTGTGCCACCTCCGCCATTCCTTGTAGTTGCCCACTTATAACGCAACTTTTTTCTGCTCCTCGTACTCGTTTGACAAAAGAATGGACCTTGCTTTTTCACTGCGCCCGACCCGATCCGTATTACTCTGGGTTGCTCCCTACATTAGTAAAGCAAACTCTTACTGTTTATAATGTGCTAACTGCAATGAATTGGTCGTGGAATTAGATATTAAAAGAAGAAGGTAGATGGTCATTGGATTTGCTAGCTTTGTTGACCGTCTAATAGGGTGATTGTTGAGGATGGATCGCTTTTTAATATAATATGTCATCACACTTTCATGGGGGGAGAATGCACTTCCACCATTGTGAGTGTGTTGCGGCAGTTCCCCTTGGTTGTTCTTCCTCCCAGTGCTCATTCCCTTAATCGATTGCCATCAATGAAAATTCGAAATCCGGGTGATGATTGATCCGATTACTACGTCTTGTGCCACCTCCGCCATTCCTTGTAGTTGCCCACTTATAACGCAACTTTTTTCTGCTCCTCGTACTCGTTTGACAAAAGAATGGACCTTGCTTTTTCACTGCGCCCGACCCGATCCGTATTACTCTGGGTTGCTC)5@HRZkr~ (2<HVcp} &3<GP^ku~$0>JU_jv'2=EQ]hs} #-8CMWcmx #-;IXgr~ (2=GQ^hs}*7GT^js~    ( 4 = G S c m y    & . 9 E O Y f r  ) 4 = I T \ f o x   ) 5 @ R \ h t  # . 9 A M X c k u  '0<HR\do%/AJYdmw!,3@GYhpz#+9GShqy+:IRco{ '3CR[hs +6AR\hr|)5@HRZkr~ (2<HVcp} &3<GP^ku~$0>JU_jv'2=EQ]hs} #-8CMWcmx #-;IXgr~ (2=GQ^hs}*7GT^js~    ( 4 = G S c m y    & . 9 E O Y f r  ) 4 = I T \ f o x   ) 5 @ R \ h t  # . 9 A M X c k u  '0<HR\do%/AJYdmw!,3@GYhpz#+9GShqy+:IRco{ '3CR[hs +6AR\hr|   #*  *3*+,&"2  & ;""8  +                #6 ! "                                                '                    #*  *3*+,&"2  & ;""8  +                #6 ! "                                                '                 S<9 %=a^aO.52 Y/O#Y-N8&^ !Q,(9bY: P) FPQP(Wa`S A- )$05@AG ;%Q$Y/?C!<N$D_`;P>E( C=#.5=B:# NH[0b Q 8TO E5* OHOF;9&- K/?C3=*-2B#,[F2SJdMO(V_NM4C*=U+)` 69,FaNUY@ 8Yb!, )? 7DWIE7 GWK)CcA 9U ^I? 68NaV"24^/=)+E1+E2 5V7D&TXB 'D/$HLE??VT,ZE1J!AI;;?X;R+Z<J4&%I>1'O>R B5$[?P4@(C!<% -('FP3I0L1(/ c3.H@H3 2D0V .6A T-.O ^1% ,T/,>88H`A D`c 3;W7D?B".Rd`Z:X<aEW`5;E:#D HOm% -`P7#YFv444 l i+H bw, `\VNd_i(aa ): w(P\5%)_#x/G|ZY8R{%)2< ;? !plf'i2f :BTY2V/>x1|Qh\uH=&=o *>8% 9QF}#p9xKG@@F+"C{"<  is2 5R^mt}1g{l yulN^/M|].^XG!imOh)yc L`u `=qE]j.qah8IAfSq= BB  i11e v}aQo]g  #]ieUkf+SW8 +P  b[p-8oI@ q @it]~%C+%xm-(  9/<MR3GM6du% %@k. 2e "=1+L?0b    ,"N) 4)% -#*@@ $>~!6+P0>,b6F  ?%Q?r! p0b7q{r dq,p],8<,NT/[E,>wM[</r[uYb)K|8FYxhp,y =@]&S_$Z}Q!,K2. &-dsxG3@)!Vg*df,S , S{6(4aA|8)QJ#.,(W\[ {a>_p\62A4Lp~ Gz'AT@Ow3>7 e8\R`m8KF ,u ~hd*Fpk73fKP QClp&H~!WOq*wsc0 ! a\n<E_K@[aw|6sn-  {)#w%e0"-xwN-#,GFE]arD S2aP8oO`w:>s?eQ<5--OM l-IQMITi1Ae^}r$`!7P s^>2O&>?bE)@NI`{ MhyW#j7o) -teF"8ob2A7dqQJHl)j`k468!j^veP|[ZC9-^2:*hvq8(0U:EfO- $zS:/x';aDMl'1lGGGGGTAATAATTTCGCTCTCTCCCACTGCATATTATGAAATGGAAGTTGTGGAATAAATCAAGTCGGAGAGAAGTCCACGATCCCGTAGGACCTCAAACTAAATTGGCTCCAAAACGATAGTAGCAGCAAACGCATGAAGTTGATTTCTTGAAACGGGGGCCTCGCTAATAAGGCACGAGCATAACATTCCCCCGTAAGTGTAACCATGAGCACATGGCACTAGCACGATACCCCGGTGGGCAAACTGACAAAGGAGAGTTGCCCTCATGACCGAGAATCAACCGCGAAACCCGGGTCAAATATTGAACGGAACAAGACCTTGGCCTACAGTTTATTATACGAGTCCAGCTCGAAGGGTAGGGTCTTACCACCAAGGTGTGGGACAAAGGGACCCCATGGTTGTCCAATTTTCCTTCCCGCGCACAATGAGCTTGTTGATTCGCACCACGGACTGGTGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-18 18:21:38 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS$hBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #tFDATA #tF+DATA #tFrDATA #tFDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~"LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISuP1AMP1RLP1WDDP2AMxP2BA!P2RLPBASuPBASPCON CPCON vPDMFPDMFPLOCPLOCBPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT ,RUNT  RUNT Rate RunN""S/N%mSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER $]SVER,,Scal@Scan3TUBEA5Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS$hBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #tFDATA #tF+DATA #tFrDATA #tFDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab2FVoc2FWO_GATCFeat..2GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~"LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISuP1AMP1RLP1WDDP2AMxP2BA!P2RLPBASuPBASPCON CPCON vPDMFPDMFPLOCPLOCBPSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 2RUNT ,RUNT  RUNT Rate RunN""S/N%mSCAN3SMED SMLtSMPL SPACMSPACSPACMSVER4.0SVER $]SVER,,Scal@Scan3TUBEA5Tmpr<UserphARphCHphDYbigphQLcphTRphTRk&zt Mirs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!PkWgtb2qi^jIMFIB)lKS|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_>BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|$q_6U30bUSZa!Ut;_f3tVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#