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I G ? @ K = = 3 ; 1 > ? < @ A ; < S ] K ; ; R G ' # < = * 3 D 0 D , % ! 9 * 6 K > . ) ( R ' $ ) : ( ; 6 = ] D 7 R W ] P s V a m q n w n v q o m r k Y z t N m f _ j Q h o u r z f V a m D T U ; 9 A E P M M @ ] V J a g c U _ ? C 1 P L J > K 3 D I X * + K P 6 G R ) @ T J e E Y @ H K Y X E W ^ h O H R K 8 ) @ @ 8 9 K G C * < V M O : A A N > E . ) 3 + 5 6 8 % ; ? < E 2 W ; 6 ) 4 : 1 K ? B : . ' 4 & 5 1 6 - 1 K B 7 D E . = ^ < J , B . : J W L ? : / / , ; # , B " . G B & = W X & J E 2 U 5 E H U 3 : C L N U V e Q S R E U : A 4 > / ( 9 : Q E = / ? N T I < * : 1 % # = 6 W D < = T < + / S P P 4 N Q : N H H H L ' ; E ? ; F I / / Q < D 4 E < D < 3 ) K P K N I ? : L ; 7 H E K ? A D X H H P < B a 3 U F ; M 4 / 8 / Y Q < @ E 4 E 9 C = < 8 J G C G > @ G . 1 F ? D 4 E > + + G K G 6 J C D \ @ J N M ^ = G T = < 7 7 5 S 2 < 3 J $ 3 3 - > E 9 G 6 M O G > 9 4 D 8 7 . ? ? 9 5 L % K < H 8 ' 5 I 3 2 G . @ / 0 6 1 ; 0 8 5 % 9 * 3 5 % 8 E , 9 A V B ? = 6 @ G @ ; 1 ; S 8 V 8 < < I Q < 9 # C M 3 2 6 O 9 G P H e A ? 7 T B d A [ Z V . T C K S > I M R O > N F ; K C K < : C F a 9 E N M P A E C # K H L K h [ U M N J N W M U I ] W Y Q M e E a N L \ W H H e l V Q ^ _ S D Z h Q D 6 U H E R N O M Y O U > ^ M S O E O a i L Y G S R = G L V F a Z Z b s ^ M f _ f K Z b U ? > A G 5 > S c I [ K _ Z 8 E R U S L L K 2 N U F E 2 G 1 @ K , 9 ; 7 : ? = 5 R R H P S G C X M A / A @ F E B G ^ i Y ` Q a 7 Y P ` G = O Q D a \ [ a R 6 P P G : X S R d p ] N M m > @ I K I l P I B N F X Y O Y : @ 8 U > : / A 5 9 J ? 5 7 S A , > 7 5 F ? C / > ( 9 7 $ S ( C > F : 7 . ) - 7 > ? W = 8 # ' 0 : Q I 4 = J * L C I 8 L ; 8 = 9 6 C A F , 7 D > @ 3 A M > 9 , ; , ( ; B 0 F & = 6 J B O D W 4 ` c L 3 W ) ? O M [ L F O = < = , I Q M J F g a [ \ > A 9 L : X B T K b S \ L Q H U D k a > S c H ? ` 9 K E I I Q F > E B D T D K V > 7 B > ? ) K / E Q Y : 4 G C D 8 3 A \ G i A ^ 6 [ = : ? / J G Z E P \ H L C O D C 4 ? 4 A > = / , 7 E # D G : 6 9 5 L M : H 8 = B 6 G = , K - K ) / 3 D 9 ; / I 9 . 8 ; 9 D 5 E G 7 L A F 7 > C & + 6 4 > F K F / I 9 D 2 @ < U ^ O 5 a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h Y I R G W B / E W = C A F J ^ N W ( F D A E C 5 L B I 7 H = ; @ < ? G S . X ; A M C 0 : O 7 3 H F E B ; 5 A - < > 5 3 ' E B 8 : @ 8 1 G . 5 # 8 J 3 S 7 B I = G X ' . 2 8 / G 2 < > D 1 D > R @ B ? H D H 9 B E - = F + * G 1 K ; : J D H D 9 1 ^ X C G C F ; % E U J G 9 @ 0 % > @ $ 5 . B . ; \ N L b 1 E ; K V a R S [ G 3 C Q 8 5 F K 9 5 . @ 8 N < . > > : 9 D M 6 K T ? 8 D C U 2 L . P S > 0 B C S A ; G ; ? C 8 O \ 4 ] ) H X A 9 D D ; G 7 T ] A ; < E V . 2 A 9 5 ; K B = = 3 1 > @ : : 6 H 0 F W 7 % D 4 0 C 6 - + G J R D 7 N I J ; O ; O B ; C + - I E H N J ? B O C ? B = J H R $ K G 1 M G 3 & R 7 A N D 5 U O B 9 > F 8 8 F % I Z S ` U L _ B ; ` 9 ? 5 N 7 P 9 . @ L B L . G Q 6 . 2 O : : I + W 7 K A E F 4 J M A E P S 9 / V @ E R J 3 A K ; J U ; K R Q O > < ? ? E H / R ; G @ e F 4 = D M T @ B 8 @ > E 9 ] = - ) 8 1 4 J = 5 ) 9 - 1 F E E 9 ? 9 / 9 @ * ? , > J N = # ( ) + % % G 3 ; : S 5 B , L F G 9 . 5 5 ? G 3 N K A 9 " + . G . ? D C B ? 5 F * @ D 6 3 7 $ 1 ? 7 N < Z 1 A < 9 - E K A > D 2 & < = F 3 I ? I N N T P : 9 F I N ; 4 * + ? J : 9 5 / 7 ; F : 9 ? 4 B ; P < / C U R A 9 5 D I ? ; F > F G A F G B R M [ G X ; I > S B Q G C L F ( @ E < E ; D T T J N / , : C 9 U > < F J . A U 9 Q E @ . L B N Q I I h @ P Q . - I = 8 A ( @ B N J J M W 7 B _ = > L [ > J C U ; < J 2 B U I N ] E D F V > R S L Q A @ D 8 > > \ @ & = O ? J O = A Z U N R 8 [ X U X ^ \ U Z ? , 2 [ A 6 U 9 = E [ G M B M F ; @ ] . B D @ O I 9 7 J , C H : ? ; > T J p T I B B h T G 9 E I < A K J 5 ? > 9 I < H J K S R L N I 6 9 G 4 T 3 ` N K K C 7 4 J B I 7 4 , E A > > I D : L 8 H < D a D 1 ? = " 3 = 4 ' : 2 R M R 4 5 8 Q G R 3 < C Y N A : N = L N T R G J Z ; ] 1 > C C 9 , M J \ G F : < : ? C P S R B 9 / Z `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f C < 4 / 1 2 = $ = ? ; < G ) @ 0 3 D 8 * L E E @ R ; @ * 0 T 2 9 J 2 P F 6 M F < W X > > ) = B I A K < S @ A C 2 D H Q 5 B C ; F E N C N 5 B O : H > 8 9 5 O : ? C < B @ F G > B d , > 6 L 1 ` H ' O / E 4 + 4 L @ B 1 I A 4 : O = E 1 : @ 6 / > : % 0 9 : ; Z E R A D , 6 B A 4 ) 7 F 4 4 $ 5 3 > ? 4 2 G a = G P S G 9 R O P O D L D M 8 * M ; R C @ L M J C f ? T M X S ? f F T Q B 1 0 7 M Q = 8 A R < V ? ? C 9 1 D 4 E K J A - M 6 ? A G = - 4 : B 4 5 @ F E H = A N 4 ? 5 J D J G R Z = J Q U M P 6 J = ? ^ D A R > 9 1 F < 6 5 P # I \ R K Z H M ; K D > 6 I 4 @ C < Q W J _ d O C S 7 $ P N ` H H K Y K C Y N ( A : : P 9 L J A K 8 8 N F 9 7 N D E 7 S W ( 8 C 3 < L & ? A D H C ) > A = < 1 ) 2 - < A T 9 > G 0 < 8 Q < I 9 < A : 0 O I M Z 5 R D M 6 Q N A E & = / 8 3 S D > D ) G - G 7 H A 7 7 5 D 4 5 / T # I F @ & 4 F H = / E P < E F ? @ " S < - X > E 3 4 W G ? S P 8 B C H G M D > P 6 M A B @ 8 > ? 2 1 > ' > Y = ? 5 F C / a F L ; ] . $ N T A H . < J F K F D U P = ; < 7 ; I B : : > U C B F 5 @ J P > : 3 ? : L $ 9 ? 5 > 5 1 9 3 A 0 Q - > L L 6 8 0 C L 4 T M X * D 7 S < S > + 9 * A 5 I M A B I Y W R 9 H N U F ^ K F J B ? C . G P D F A N J 7 N - C F M < / 5 I 6 9 8 / ; 9 9 K 5 > = O K 8 : ? L < N 7 1 > @ U X m ? ] P R H 6 4 8 6 E 8 U 9 . A C 2 9 . F C ? > : ; 7 F ^ 9 O 9 L = I J ! 1 A E ? 6 8 V L O C B ) M L + 5 ; / ; ? O G N M & ? = @ > ) 5 B : < G @ O H E M Q < 9 B M G ? : % ? F T < . < - ? 6 7 D I Q R @ 7 N H 5 C G H > B A 8 3 0 E I 6 1 L M E 4 D 2 X < : < < 3 1 @ / - 4 L P ) L 2 ; 8 8 5 > B J 9 6 9 < = = K L 0 G 9 ? = J / * - > 9 J & 9 G 7 ( 5 M 5 : N B 2 & @ N I 6 B H E ; @ B 0 H 7 ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 14e9a7af25fc4dcc8849415d81ca1a39Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-18_15-19_0736 3130Seq 12475 12475_Costion_K1319_matK_F jmadera 96-Well K1319_B4KB_3730_POP7_BDTv3.mobSeqPop7z>`H?
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KB 1.4.1.82018-05-18 16:50:17 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
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* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B4 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN v ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab & FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
4 P2BA > P2RL
* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B4 Tmpr < User phAR phCH phDY big phQL c phTR phTR -Dq.$+WH x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&reA 03]^gi7$7nA\w/nb+:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]UF+t4on5A|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhI3yuX.mU(2hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;= AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN v ASPF ASPt ASPt AUDT ` ` 9 B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab & FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ C LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM
P1RL
P1WD
P2AM
4 P2BA > P2RL
* PBAS PBAS PCON PCON PDMF y PDMF PLOC
PLOC
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ) RUNT 1$ RUNT ) RUNT 0 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE B4 Tmpr < User phAR phCH phDY big phQL c phTR phTR