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"                            P ,,,III^^^{{{&&&99DDDNXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7 "#"##!!!   Z[ZRRktt(('222===GGGQQQZZaddmmmvvv~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 30503ff573664504b39182d1bb3290c7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-05-14_17-06_06913130Seq1241312413_Costion_K1318_matK_Rjmadera96-Well K1318_H10_RKB_3730_POP7_BDTv3.mobSeqPop7zIa{ n07y} TO|K\]>sE 1x0q*'n/piuy`(yNi21O Yv#3q3uiM7$&<H:SqNT;!+-OkJV%$q7^|P 1(F uQztp<!9B^S:W+RiD`?t 0 e!Y':-.3;oE~RbuozhWwG[96-y$*Yq y ( k#: o h { g  X[e> & y$!`'/]6=wBFGGGFCA?:5/)a"d 6VII? uYF>"R/R?s+Q $A_lV_+8 gBIs~~zts=t!Cr~iloc8"3aw"])%J  :YJl9 "! h>t[( R8K/#D@U-AtzE  9d}W.R{u }\3 "sZ&5zmB anlX@'=a|pN%8SU=DUV3/Ee,z! &Rx}W&O:W `8"  (32& =WuS6I_, -82 6m`+%z;iV.=U&C`\\m,<B rrZ!7m La# -Hj~J5VdY>&":f^, 0a] r~)+rD$ 8ZgTWca@V Ux@ abu.k% -CRTI7)%),&7g]1 (9B=, !:IH8!  &+(#$ &*"  Qu=4r/F32PtD>veLtT|`.N2k{;:t+cp`VqiM|>IH^0g {Y9-GA^F'm(%`$"@u3As;{Y,Hek]C&  )`Y. 3XxnI#  .63'   (Ov#l wTmb$ Y Kp_' +Tu{a2 +v: 2zX2gc#-@2g{GSj8$*7_'Is8mHf8kpP.  ;QZQ: <^v{kK( '@S\_dt:}hr  EL![b5#N| %Us1B4OY'm9O~00ZR0\l4 1\z~hC*;?4 D\X4q?Q85ShhP*FepeM5,3DRSA$=[kiZB*%xH)|b     /h_ZiA#Kk>An:u*0N\*HwR=L=JL01kb: ==N"^<ws@1:2! 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E0aK& a[Vc"# ,]/IdAG L=NNE4DI^`F[&Q '^/%JI \25LaI3\R(Z9 ?<x0SM0ao?!V6*R!arLT Mm1yg;5{\Q0I$i+T x:-aCZoM4W)\q#@%uS:a da@"Jb_R0,kP'X75GCN:%  >],) P%7v,Ep "dp+#prQfpg]XxDwo cl2_ltH=8}=YY:pK-65%:?Z_eA<7  XVN'1S60g51v|BU:tE'IW;sn#u{ 'Gy`Wci{kO/MsoLCzGGAGAGGAATGCTGGGAGGAAAATAAAAATGCACTGGGTATGGGGGTGTTGTTTAGCCGTATGACGGAGGTCGAGGCGTATTCCCATAAAGGGGGAGAGTTAGCCAGGCTTGACTAAATTGTCTTGAAAAAAACCTAAGATATTGCGAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-05-14 18:37:29 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtBASPtBB1PtAB1PtABCTSUBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,pXDATA ,pX=|DATA ,pX\DATA ,pX<DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~SyLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISH$P1AM,N3P1RL,MP1WD,O_P2AM,QP2BARP2RL,PPBASHPBASHWPCONLqPCONKPDMF|PDMFH;PLOC,JPLOC,IPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT $RUNT  RUNT # Rate RunN""S/N%HSCAN3SMED SMLtSMPL pSPACMSPACH4SPACMSVER4.0SVER TSVER,,nScal@Scan3TUBEH10Tmpr<UserphARphCHHRphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtBASPtBB1PtAB1PtABCTSUBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,pXDATA ,pX=|DATA ,pX\DATA ,pX<DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabc\FVoccKFWO_GATCFeat..cGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~SyLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISH$P1AM,N3P1RL,MP1WD,O_P2AM,QP2BARP2RL,PPBASHPBASHWPCONLqPCONKPDMF|PDMFH;PLOC,JPLOC,IPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT $RUNT  RUNT # Rate RunN""S/N%HSCAN3SMED SMLtSMPL pSPACMSPACH4SPACMSVER4.0SVER TSVER,,nScal@Scan3TUBEH10Tmpr<UserphARphCHHRphDYbigphQLcphTRphTRFB}}Cg/D&Wr`s;bqz^]/r$^.>6pe:*>^Jp@48Pl: &Pwi~69b[dWUlh{.4eY\`;w>xPqL6`nCRNX}a/JBcw*jEth@t#Rt+7+:}JcLL-GIA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]kId`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{c|5<&n5N`!1;U*W]S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve!@;Ps>4]F%65r#W3}hxv_1=|8Ifz2wHiD(g=0ia}yI?t_tG|T`S'_[PTcM#yKAu22`ArIxT6e&?bFW3|>oW~!Te4;,: TZc:=?GVb"[\Hz+d3AFKep$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VL&p?)_QQe|vH[p!hjqf[MH-YtDSYcJ|_nO*rSq,*c;&Dcb[6(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9bz)4>@:s<\P:miPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtBASPtBAUDTffqoB1PtAB1PtABCTSUBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA ,pXDATA ,pX=|DATA ,pX\DATA ,pX<DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabc\FVoccKFWO_GATCFeat..cGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~SyLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISH$P1AM,N3P1RL,MP1WD,O_P2AM,QP2BARP2RL,PPBASHPBASHWPCONLqPCONKPDMF|PDMFH;PLOC,JPLOC,IPSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT $RUNT  RUNT # Rate RunN""S/N%HSCAN3SMED SMLtSMPL pSPACMSPACH4SPACMSVER4.0SVER TSVER,,nScal@Scan3TUBEH10Tmpr<UserphARphCHHRphDYbigphQLcphTRphTR