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L}1 } r k h l E W [ a K 6 = H G V 7 H < + H > A 5 S 6 = = 0 E I < : : Y U L ? G X , O 8 9 d B 2 9 @ = I W c b S _ q i } m @{<B|wt' i n o ^ a Q p h v g i E L F M 8 N Y X K R Z U G g a l Pj* E4 ~ f T b [ N W Y J N E A G 9 C F R = 6 ; I G G A ; F I H F = = 2 3 C 8 R > 7 ( 7 E : K J G = R ; T R F G D J T G D G U M 6 O B F @ ] ] i n r r i s U,tH,|Et$ y q r b l e f m n 2"T21n x ^ n a J \ Y W X Z k e T j ^ P F T @ W F i ` r Y f k (m.D fLF|#yo['@$ ~ r M Z e ` F ? b ; F I T O T P T D ; D G ; G @ A < , < 4 S E < M ; K K 9 D @ O 6 6 < O 2 8 1 ; 5 J 5 : ? 1 . = 4 & D A 7 / I H C 4 7 9 * B , 2 / ; A - K N T D F / 4 > O 4 Q N Z N = A g c b M h L d z X7.B+> i { p X N ] ^ Y H K Y I . Y . , J 6 7 C W L T G A \ D > A 6 G C I C L K I ; D M > 1 Y Q E A O < _ : Q P W ` n l a q l ''HN)<} ;G u~* F /&A3N! q}|,(T m d S g V l N [ \ J I U E P \ N ? G R = A I E K > > D F [ = G 2 B @ I 5 K @ O B G < C H 1 Y I 2 < D 8 E 8 R N / D 9 / - 1 < 9 ? 7 + 9 I E > 1 ) 2 D 0 6 @ F = 9 / R Q : S D / > 6 B = D @ I F @ 5 ? , P 3 5 : 2 S G E C B U E P = F ` M 9 N W 4 E M ] N ? I D F I L a Y I M j g q p k n JYaHcKL\zTK|cw[ XSkhC { d j r S f j W a = [ X Q R U M J 7 D < > ; J . 8 0 : 9 0 F V ? ; C 8 C @ U D < @ > ? < 8 N E 8 A = < D ? > * C = F F ? 9 ( ; 6 = Q M D 7 H : [ E O ; < L U Y b V b : 1Lk[1 k u q h W J Y B D Q Q G V N 5 F P G E G C 9 > @ K a J ; J ? 9 6 = J 3 ( , 4 3 3 3 P > A 9 0 A : ' A 0 > N C 1 , 4 < 6 ' > 3 4 9 8 ? 9 * ! / 3 0 = . B @ , / ! + ) 5 : ' ) / * 1 1 2 1 6 7 H = @ : B > @ C 9 F 1 9 ; K - 8 7 ? 7 - 7 D / 5 2 * 3 A > 3 3 Q 9 + = . ' C 6 3 ? 9 , = - 3 5 5 2 5 J D 5 9 * 1 5 C 8 3 ( > = 6 F C N - < 9 9 ? F 4 9 C : = = 6 3 ! + ) 5 = > 9 ; A 1 4 0 8 * 9 9 E C 0 6 3 ? + . 3 6 A 0 1 % , < ! 1 8 > . . 1 G G ) ; 7 6 5 3 1 6 C L ? " 0 : < @ 6 F : * ' < > / 4 ' " ? A / + 8 2 5 9 ! < 5 4 3 ) 1 = : # 8 C - B # ; A 4 I E L 7 , / B 6 B 7 & + : B ? A B 7 K H K : < 0 D = / 9 @ ( : 9 3 2 * 7 ) 6 0 - = < A F 1 : 5 ' 2 3 6 9 < / 8 ; H 0 F I A : B L / 0 0 A : T 0 ; E 8 ! * ; G 5 3 $ 8 ' . / D 4 - : H 6 / 0 M 4 3 1 7 ? + / ; ) 2 G ) 1 ( = 7 9 = 8 : D ) - : 7 0 ? + 7 < C 6 E A 7 ? & F ; G : ? 9 / H ; A S I ; ? 3 L 7 7 9 L . . 7 0 3 M H S Q N @ 1 P S ` E N X Z Z V n h v S{b}OeCe&B`(DVg3 t k z k [ h a n T k l k v n d r z <#1C7Ls<*69pd3 e J j h g m Y m N L Y G F G K A R E E ; F P [ * 1 2 G . 3 F @ D ? 0 9 ; < : C G = C 8 3 > 7 9 G B A D T H B @ 0 H 5 > H C \ B F 9 E > 8 1 9 1 ) : ; * 5 , C : ? ? ? % B ; D ; / @ . K E D A X ? @ B I L Q O O K ` P Z a i p s w.,}wD l
& '"2 !nss8l5 u w v W _ H K N M Y U A O J M R Q W P > > > B E ? R 4 = 2 ? C : = ? R N @ @ = J > 0 < : < < = 5 B > F = L 8 / 7 8 B 7 6 C 8 * , / 4 O < ' 4 2 = 1 / - 2 ) 8 @ - ' , , - & 5 < F = - 0 : ( ) > B 1 4 ; , A < ) , + < F 2 ; : 6 2 2 6 > 3 4 E 4 A + > 9 , G ; @ B > ) D 5 C 0 < E C 5 0 6 1 3 5 7 $ 7 5 7 ; ; : : 8 = 5 C 5 + 0 9 H 3 + 1 . 9 < B & A , 6 7 - * > B < D 8 5 ; > A K 6 H T < B < V 6 L T < I H D 1 ; 6 I I Q R S \ M ] A Q M H l n | x K
WR7mbfR r t ] h Z N Q Q N P C X N Y ? ; G D K A = F 3 8 4 5 6 - / @ 2 = 4 " < > @ 3 : & * 4 3 ; C @ , + 8 , 4 4 " 0 ) + D 2 7 * 4 - ? # / ? 4 . < - N 6 ? ? ; C 2 1 9 7 9 4 - 9 8 L Y P 4 G B , @ > < ? 2 A > 8 F > ? I C J V C P R G $DEaH6qU p l g b P P D J + C ; ? A 6 B = @ A C 2 > 4 3 @ 0 @ = 8 1 5 ( 5 . # 4 = " 9 6 : 1 B 4 ; $ + > 0 / 1 " / ; ; 3 5 ? B D 6 F * * S = 0 3 B @ ) 2 3 6 0 6 * % ( ' - , 1 9 7 ) = , * C : 7 ? A 8 8 A 8 ; < D 7 8 8 < 9 / 5 = 4 . ( 9 5 8 9 : > > - / ; 2 1 ) C A H . ; > K ? ; G C < 9 R K M K P W R \ ] i o v f!5DnAN } q m k a T V N K B e = I H L : L 9 H J A / @ 4 4 8 @ 1 I 8 : G 3 : D 7 A ; G C 8 N = 0 ? : C E ; 4 @ L 1 ; : $ 2 D 3 = # 8 C $ > C > ' C = ; ? ; F ? B O L U H M X i w .n?"|Ke)Y { g h ] V ^ U D > a X s s 4vF 4/L s d ] K P P R U ] T 3 X 3 R 0 / > J A 8 7 + ; ; 2 + + 3 - . D 2 9 , 0 < 6 ! = 1 8 % 8 3 1 . 6 < E 0 B A # D # 1 D 4 , , D 2 - ; / @ A - * C 3 3 & + 6 / < @ 7 . ; @ : < E 7 ; . 6 F 1 < ; R ; U a q d I^5R]w`& i l R n ` o N _ N H M d \ i i Bs ,tXS/"uTB'A[T y ~ b f o M P ` d M Q < S R , 1 6 2 / R ? 1 E : 7 ; G 8 A I E ? = 9 = H + G / 4 5 * C 3 - ' < ) A = 4 8 1 1 : . , A E 3 % E 1 * ) 1 * > . : 7 2 5 9 , $ 8 1 7 1 I 6 + 5 V ? B 1 B B E D D : > @ O f x `}\UP) { l h e Z ` 7 Q 2 T L : L @ ? 6 . J F I ? 7 < = K B : K O 7 4 0 B + 5 : 5 2 6 : , F 4 ! 0 * 7 5 3 9 3 ( : * % 4 C 4 & K 8 < 6 1 = = 2 ; / $ 5 0 B ' 1 2 B 5 + 6 I 1 6 @ ) B ) 5 0 > , I D ; - ' B 6 0 + B $ 4 * 6 E " 3 6 0 2 + % & ? / 4 ; ) 4 & D 6 % 6 @ 8 6 2 * 2 * - 2 3 . / A G @ / : ; I < D ; 9 - 5 Q T 8 [ T K 1 Y _ b s
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c86d96d70a6a47c0aac99c805be09d53Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-14_17-06_0691 3130Seq 12413 12413_Costion_K1318_matK_R jmadera 96-Well K1318_E11_RKB_3730_POP7_BDTv3.mobSeqPop7z B C E H M T \ d k p t w w u v z ';JU\dn}6Rl6g'7Ja{f?i2wT1 yE { k ^ U O K H D ? 7 0 + * - 4 < B C @ 9 0 ( % % ( + / 1 3 7 > F P Y a f k q y w o l j g ` W N F A > = < 9 4 - %
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KB 1.4.1.82018-05-14 18:37:50 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS y BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA *\ T DATA
*\ T 9T DATA *\ T DATA *\ T DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE W LAST ~ ~ wx LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 7 P1AM [ YE P1RL [ R P1WD [ _ P2AM [ mg P2BA [ [ t P2RL [ f PBAS [ [ ; PBAS [ [ 7 PCON [ [ O4 PCON [ [ K PDMF | PDMF 7 PLOC [ E# PLOC [ >m PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% 7| SCAN 3 SMED
SMLt SMPL p SPAC A[ʝ SPAC 7 SPAC A[ʝ SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE E11 Tmpr < User phAR phCH 7 phDY big phQL c phTR phTR ́ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS y BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA *\ T DATA
*\ T 9T DATA *\ T DATA *\ T DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab \ FVoc K FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE W LAST ~ ~ wx LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS 7 P1AM [ YE P1RL [ R P1WD [ _ P2AM [ mg P2BA [ [ t P2RL [ f PBAS [ [ ; PBAS [ [ 7 PCON [ [ O4 PCON [ [ K PDMF | PDMF 7 PLOC [ E# PLOC [ >m PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% 7| SCAN 3 SMED
SMLt SMPL p SPAC A[ʝ SPAC 7 SPAC A[ʝ SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE E11 Tmpr < User phAR phCH 7 phDY big phQL c phTR phTR "3Z2VW)>ZHm? V<(?:134]3[16Qu5r`xC>2aZ!ly^A9Jz?gMBI+\$jn18A7y!H#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;{v3Ah-8^#~C=O\rHU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nX>zt %v1?{6W8:7F}wxSB#_{=pQ$+Sn0&'*#))MR]N=]TAO$Cs-1G@cJ-omMOxq6agI)Y_bO?C!.tk,8#V>Ax7cXF$hHz))O1"Kdnh-QX`|Pr3PF4>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }Ou>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["[87PFM,xS5hhgloc|6v4g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39qmGT(ANsQGh_n9Nfcd]R039OQJM~V[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %> m PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% 7| SCAN 3 SMED
SMLt SMPL p SPAC A[ʝ SPAC 7 SPAC A[ʝ SVER 4.0 SVER x SVER , , n Scal @ Scan 3 TUBE E11 Tmpr < User phAR phCH 7 phDY big phQL c phTR phTR