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n W Z M H D C 7 5 : 0 < , 0 ; - < 4 ' 1 ! 6 ) ; - ( E , ) 2 + = 0 5 6 7 - & . 3 7 4 + ? ; 5 0 2 4 ; + 0 4 = 8 . + 4 . ^ G E N P j 7 } n a _ ^ L D G 4 + 5 < = 1 ) , 6 . 8 4 ( 8 # - . 4 / " : 8 0 * " 4 % # , ) 6 / ( + $ ! 5 * / " - ; 0 . 3 , ? 0 $ - . 0 % 3 + . / # + - 0 + $ ! . 5 / , . ) 1 . % ( ! 1 $ % . 1 & ( - - # - ) 2 $ ) / . , , - , 0 " 1 ' # 9 ' 0 : % - 1 / % ' 0 ' , ! 2 6 1 + , ' * 3 6 C 1 " % 1 / ) ) . 1 ( # > 6 - + ! ) 5 8 , ( ( + 8 ' ' - + ! 8 # , 4 % 1 : , * 7 9 ) * 2 * / , ' ) ' * # 1 6 . = 6 A ) 4 A ' ? 2 2 6 ; 2 % 0 4 5 A R J K Y U /<bjJ% z n O M G \ J : J E 8 + E A = + C 6 @ & * > & > ( < 1 8 @ 1 & = , 1 @ B + 4 6 W o m } [ L _ ? > 5 G 6 6 I 9 7 + 7 / 2 * . 1 % * 9 + + 1 , 3 / 7 2 9 8 7 , F 3 . 9 2 @ - 1 3 8 3 = 4 6 ) 2 , 3 3 - 9 & . 2 ! / $ * 3 ; ( 5 8 = ? 7 > D J Q R { -6" u m Y [ P [ X D L K e K o p ?ciWa$ +NttVF m q _ c Q > ? ] B ? D = @ : 9 9 < ? / 5 0 < 0 - / B 4 E : > > B H 9 ? 1 J ? i )0, T g I ] [ > B D O \ r g t V R A G G D : 4 > < . 4 C 1 , 6 8 @ 1 3 C @ / + ) ) 6 9 & = . ( * / 7 4 . 9 ) E 5 / ) 7 0 . ) 0 # 4 ; 7 " 9 / : # + ? 3 + 2 + , & 4 * 0 5 . : 1 8 4 7 ( * + ) A - A 3 . $ / + & 9 $ , , + . 3 0 , : ) * ) 2 . . , 8 ; ) # / 6 ! 4 8 ( . 1 1 + , & 4 > B * ( 6 : " 6 5 % , E 0 ' / / ' 8 $ 8 2 1 A + 2 * # 6 ! 6 ( < + & / > 7 G F > a [ m ) z k [ Q D P N I = 7 7 B C ' , ) ( 0 - 7 $ 9 " 4 ) 3 $ ; 0 , 9 3 8 > A 4 : @ e _ ` Z M O B O G 9 5 @ 9 M 8 O @ . , : - 0 + - 6 - 1 + 0 & , 3 + 1 / 9 ' 2 8 6 2 - A 0 ( 5 + : 6 , 7 4 = . 1 3 ) * , 0 + ' 0 % ! * = , 2 ; % ) ( 9 2 $ # ) 4 , * 7 " + % - & 2 ' ! ( : / 7 1 + ( ) % ) 7 * , 7 & . * ; , < + / / 4 ' 5 0 2 9 2 & - - ) ( % . , , , , + ) 1 9 # ' # 3 = / = G , - < 8 5 ' 1 3 + / 3 ; - $ - 0 7 < 4 M R ] q )2+ 9 s b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k z r o e T I N 2 8 > 3 0 7 0 % " 6 7 0 1 / * # ( A + . 5 ) , & 4 ! ' 4 ? ; 6 ( . % / 8 1 ! ' ' $ 6 ) ! ; ) ; - / 0 7 + + + 1 - 7 + + 9 & 4 * ) 9 / . 0 7 + , " , , - / 6 = < % 0 / = - " ( 8 , 9 ' 3 8 2 4 3 5 / @ : 5 > * : B + 4 8 = 3 - * % * & 3 8 " & 7 4 0 ; 3 , 9 0 + " 3 * - 1 3 ( # . ) 0 < ) + , % 2 / 4 % . = ? : @ E V a y } - z h j S c R E A 7 > C 8 B ) < C G : B < 4 * 0 D @ , C D G j y } c d K P B J C N ; = L K ? K g ` r t _ Z K R S D A S 8 , * ; 6 7 E 6 / 9 ) 5 % 1 : ( 9 6 - + 8 , 1 8 . * > & 5 * ( & D " 6 , 6 " 1 0 + * / 2 . , 2 7 2 7 ( 8 6 2 ( ( . " ; ' & . . + 8 3 / ( . 3 & / ( & 0 / 2 & 2 < # / / 9 9 9 = 2 1 7 / M R [ b } ~ y j p U V J > 9 E < ; . , 0 3 3 5 . % / 4 ( / ; * 0 2 : . ) 6 . $ ( ) 5 ' 4 9 . / * * 9 , ) ( " + 3 3 = = 0 ) 2 # , + * $ , ! * - % ) # 4 1 . 3 ) ( * 0 ( * . 9 ( * + ) # 4 5 + ) , ) + 3 % 5 0 & , % $ 6 . , ' & & - : $ ) % - 0 ' 1 " ' % " * / 5 7 ! ' - % ! , $ 5 ) 0 * . . & 0 1 * 0 5 3 & ( . $ * % 0 ? 1 $ + 2 - H B V _ p ~ t \ P P A : > : ( 0 ? % / " 7 ' : 8 4 & 0 0 4 ; ( 4 B - & 0 9 0 ( $ 0 : 3 . 8 - 4 9 7 1 # 9 : : 8 7 F M f } l _ [ J C E 8 J E ) * 0 . " 2 ) - 1 / 2 3 * . 4 . $ / D 2 5 : ) - 4 / * / % 0 % 2 0 ) % 2 + 9 " ' % 7 " 5 * % 1 % & ' 3 ( 1 0 " 0 % ( * ? 2 * , > ( / 8 * / 0 . ' / $ ! ! $ 2 ' 1 , ) 4 + - > / E ? E S P l s n t U E > C H 4 @ . . 8 ' 5 $ ) 1 , = < " " 2 9 = - & ( 3 5 / " 0 5 " " * 5 8 / , ! + & 8 + 8 . + 0 # " ( $ & % , ) ; , $ 5 % 1 ( ! ( 1 " 2 ) 9 $ - , * * $ - ) . # + 0 , & ( ( 4 ) % " 9 * 9 0 " , " ( $ " 4 1 # % " # - ) % 7 & 0 1 4 2 4 2 & # - / * ! ( ) , / , , $ & = . - / . I ) 8 C C Z f m z l n Y I P N C @ < H : 1 . 2 ? % * 8 / 9 # * , + & ; ! , ; 1 * 7 3 < * - 2 4 $ . / ' @ - # 6 9 O 8 = Y X l o R V L @ L ; - : < + 0 & > 3 . D 1 : . ' . A 7 5 = S g } x q l j P F D ; 5 E 8 ? 4 5 - ) 3 ( * ) 8 + & ! 5 2 ! > ( $ 1 ( * 0 ) # ' " - " 4 , 3 ' ' " 0 3 & * $ # " 0 ' # # % % + 1 ' + / + ! % % ' 1 ) , * ! ) ) % - ) / % / / , 0 ) # 1 + % / % & 4 + % , 5 + ) ) ' & $ * - 2 + ' , 8 % * $ & ' / $ 3 ( ) + , $ ; ) . - . 7 " # " ! ( ( ' & / , # . + " . , 8 1 6 # ( 5 ) ' ' . ; 2 . 3 B S ^ k ~ ~ f a X : > = = ; H D : 8 ) 2 * . 0 7 ( * 9 1 8 , * 6 . , . / ' 2 & + ' & * & % / 0 6 ) * $ 0 . , + $ -
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c5b90b4e338a4867a1795922fede3b45Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
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AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
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00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-05-14_17-06_0691 3130Seq 12413 12413_Costion_K1318_matK_R jmadera 96-Well
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M } U @" ' $ w P b ` $ 9 z k q \ > q * L4 % 9 Q 8 1 i8 * w @ U. [ N M{ - A ? > OT) . * & 6 p S7S C s m w MF9m e KF n I k C - #*S@ 7 n{ Y F 2 ) " Y = ; u r J 4V (7c Z RH / e`W Wp- ~ C RXZZ^ tc I2,<h J pY r a(| XU w m ? 0 [ }4 x 0_ $ U 1J'ml\EkMOC=G b5E & pv@ M M ] $'QB { ;$Hs%uAATTATTTGAATTAACAATATAAAAAGAGAATATACACTGTTATTAAGAGGGACACCGGCGCATATACATACCTATCGAGCCCTTATAAGAGGATCCTAAGTATCCGTACACGGCTGCACACCCCTAAGAAGGTCTGCCGCGATACCTAGGAGAGCAAGAGACTGCGATTCTATCAGAAGTATGACCAGTACTGCAGTATCCCAAAAAAGGATAGCGACCGAGAGTAGGTGAGGAGCCTCGAGGAGGAGGATACACAACTGCGGAGTGAATTGTCGCACCAAAGGGAGATTTTGCTCCCCACTGTACGGACGGGAAGACTGTCAGGATATGCTGAGGCCTCGCCGGAGACATCGTGAGTCCGTAATGCGGTGGCATTTACACTCGAAGTGATCCCTTCGAATACTCCATAGGAGCAGTATAATCAAATTAAACAGTAAATCAGCCTCGTTATCACAAGCATTACAGATGCGACGGTTCGTCCTCCATCAGAAGGATCTGTGGGTAATACCTCGCTACTATTCTACGATATGCTATACACCTTCATAGGTATGCTCTCTCTCCATGAACTCTACAGCATATGGATATCATCTAAGAGGATAAAGGACACTTATCCCCGCCAATATATACTCACACTCATATATGGGAGTAATATTCAGTTCCACATTATTCTCCGATACTTTCCCCCACTCGGGAAAGGTCTCTACCTCGTTAGAATTATTACTCTACTAGTAGTGAATACTCTGCGTAGAGTGAGTACTCCACTATGGTACGCGGAGCCTGTATATCGTATTTTCATGCGCGGATGGAGAGGAACTAGCACACTAGCTGTGTCGCAATAGTCTGTAAGGAGTATTGTGTCACTCATCTCATTTATTGAGAGACGTGTTTCTCGACAATTTAATGTGTAAGGGTGTAGCTTTATATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-05-14 18:37:10 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS . BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA , X
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DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE + LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS D P1AM : i P1RL : a P1WD : pZ P2AM : ~ P2BA P2RL : w PBAS H PBAS D PCON ^I PCON Z PDMF { PDMF D PLOC : Sr PLOC : L8 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC D SPAC AW SVER 4.0 SVER # SVER , , n Scal @ Scan 3 TUBE C5 Tmpr < User phAR phCH D phDY big phQL c phTR phTR ǁ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS . BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA , X
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DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc x FWO_ GATC Feat / / I GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE + LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS D P1AM : i P1RL : a P1WD : pZ P2AM : ~ P2BA P2RL : w PBAS H PBAS D PCON ^I PCON Z PDMF { PDMF D PLOC : Sr PLOC : L8 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC D SPAC AW SVER 4.0 SVER # SVER , , n Scal @ Scan 3 TUBE C5 Tmpr < User phAR phCH D phDY big phQL c phTR phTR [4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&=L!23]CUTT5ab9HU'EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NH!P$^g)DwD$.{y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t\,FL$&\=3Uz={hy~:<:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXvbNhsrU5-uvCbbJ_<$AJu"#phv*d,S:m?(=hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;= AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS . BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA , X
DATA
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DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc x FWO_ GATC Feat / / I GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE + LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS D P1AM : i P1RL : a P1WD : pZ P2AM : ~ P2BA P2RL : w PBAS H PBAS D PCON ^I PCON Z PDMF { PDMF D PLOC : Sr PLOC : L8 PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT $ RUNT RUNT # Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL p SPAC AW SPAC D SPAC AW SVER 4.0 SVER # SVER , , n Scal @ Scan 3 TUBE C5 Tmpr < User phAR phCH D phDY big phQL c phTR phTR