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K < G @ < @ = 7 > 9 . C : : . 7 A 2 I J ? ? A ; A = = : S S Q P M S R F E O T W E = M 9 N F M L I B ? 7 H L U K H A C 2 G I < L U C X I W U F O ? R K 2 B 5 2 P ? 7 > 2 : 6 B ? H = A ; F ? C 6 > T 0 K ? L O : : ; F D C 8 J J S 7 . C A > @ / ; * A P = D H C K S G P G P U K R T V X L A O > E ; K R Q V O R Q G M L ? L B : @ D C H ? I @ G ? E L K X @ E E C [ F : F @ H A 8 E < O B K I 9 [ Q J P M M E P M S J V J Y S B ^ X S V Y D ? > 7 A N G K A T E H 1 ; Q H 5 : 7 7 D N T K E b J K O Y S M L U R d S S T L B P Q N R Q L R \ L K ` F F I J H Y E a L c ] X S a X R e I O N Y M ? L ^ Z R R [ [ \ S O L R Y P 9 N J D B T K R ? O T e R R N D e Q P L A 8 I A B I - 8 T O C L I T A E L O I V I V Y f P [ b \ O ` Z K M T P C K X X G ^ U Y ` [ W E V B E F Q ? c L C ] H S E ? [ J F T D Z F L D Q T : ? 6 8 [ ; S X H F I I L N V > V R e G Q S W N ` Y X A P O @ N H G A 1 9 D I G C A ? @ > ` ] Q V D Z Q S L W V O S I F G X A U R K J \ i g G S ? J ; W 9 5 L ] O H B O Z V [ f E ^ C Q \ ] d K R X R H X Z U O X ^ [ H V L W E V S ? Z U M A L O O M A I U D G R ? V J J Q I 1 ^ G L F ? M N ^ O W ^ _ d i W N S Z S R E f P Q \ W _ X \ H S P S K S T O _ R P g f H H a S W [ F l e a \ f ] c L c @ S U d J J Z S ; : N D C C M c I D O Y K P L b c Y U b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` E J T P 2 : : 2 3 ? 0 3 ; A B A O G P B : > R A O I @ O Z F H Y H T O D < E 3 @ = O H . G = L > ? O = / . G A H 2 ? L 7 E J > , B B _ F K N G V I _ M R O L = @ N ? L L F I > O ? P U V N = Q J E W / > > 5 O P @ T K O G D 8 8 : I A P @ G 4 Y O R K K E B A M : 2 7 N B C J I > 7 * D . P M 0 L B E ; Y G A N A M B 9 R _ : N > S K S C > O N 3 I K = < L = : E > K = < ; . 5 ; 9 F ; . 7 5 0 9 K 4 G D G K F P N / S G C H I Q I , @ 5 E K . C L ? < 7 ; 9 A 6 D 8 2 ; B < > 6 8 8 L @ @ 6 J = L K I N I F F @ B 4 = B L A < D ( 2 3 D B 2 = + ; ? / ? < C 6 D 5 L ; [ K ; G 4 H J J @ H C I 0 7 7 ; N 6 ; L @ C P L D D M F 6 7 / G 7 7 B 2 8 @ 9 < F ? ; - C B A - @ 7 = C 7 @ 4 6 8 6 G F K N ? I Z Q @ P = K 4 3 A B = 7 > < @ I > R D 9 2 Q G S < Q 8 I 9 E - : : 5 B N N A E I C : C ; B 3 9 B 3 A Q B A / < 6 3 > E ; ? L = 5 @ U D > G 8 B C 9 G < F H ; 2 6 3 O / A < D > C B I A C D 2 ; 2 O F ; A : 4 S < F ? E I < = H @ A N C 9 E 7 = O 7 6 8 @ A U H 5 Q T ] I M J L S _ G I J ] W P B L Y E K C @ F I P L M E M C L 9 R S G C V Z c X U ^ F U T V F Y c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c N Y N G B J H L Q G V W I R I M I P c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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5fefa06e479842c0a1ab27e7cab24f3aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-27_00-59_0589 3130Seq 12310 12310_Costion_K1364_matK_F jmadera 96-Well K1364_G11KB_3730_POP7_BDTv3.mobSeqPop7z <o Q n#D^{`[
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KB 1.4.1.82018-04-27 02:31:30 -04:00AEPt \ AEPt \ APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #& DATA
#& DATA #& + DATA #& N> DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE S LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS ql P1AM y= P1RL w P1WD z P2AM } P2BA U P2RL |I PBAS rb PBAS q PCON v PCON v1 PDMF PDMF q PLOC t PLOC s% PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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SPAC M SPAC q| SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE G11 Tmpr < User phAR phCH q phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt \ AEPt \ APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #& DATA
#& DATA #& + DATA #& N> DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE S LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS ql P1AM y= P1RL w P1WD z P2AM } P2BA U P2RL |I PBAS rb PBAS q PCON v PCON v1 PDMF PDMF q PLOC t PLOC s% PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT ;, RUNT RUNT ; RUNT & Rate RunN " " S/N% qd SCAN 3 SMED
SMLt SMPL
SPAC M SPAC q| SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE G11 Tmpr < User phAR phCH q phDY big phQL c phTR phTR ZsbJEP'WZ]FO{7I{6Emc9_lN#j`KJX?-'?C,F4te?t;t*2]>57_X~GjW<72s-@M+9mW'>YP~jM.9Wc@>(Jt6%> :L-Ev!$V@AK1ql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W?p<;RAX(pAjw[~W@0$.9@_05a=gHFhwD~ ')&o*5V3"WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$b!;|dxU[YCC/3[`kI ?"U27`N`3[)|$X[@5+W.mH!g&6]rG&nak'eaK|`h3/[zeqr[&Xj|82Z~9}__';_/e"^=0`VZI;P>W+YYo%+0|5Q1V?ORJedjY{mwYBTU;CxhKn6[MHSVi220b_'{aCA{%?_CjFDhECel7B]%jxxsWDy4<|O{^Mm[*&`_@-|T+a6oGhnWe^kQtA+UZ~en?,}&Dg8YauBQBhwD~EG7&o.jax]CR"lWW .C&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+b-MSl!LZUpE9W?lQD/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWDZOv'5d7`Ed,{w.P&-Gt?E>@d&r&iox~*4fC\*WiE&mi!\.~&|f&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k'eaK|`Bt#2ze/z,(,y]18`F7IOo_3@/C 4O$ AEPt \ AEPt \ APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT b b B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #& DATA
#& DATA #& + DATA #& N> DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE S LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS ql P1AM y= P1RL w P1WD z P2AM } P2BA U P2RL |I PBAS rb PBAS q PCON v PCON v1 PDMF PDMF q PLOC t PLOC s% PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ;, RUNT RUNT ; RUNT & Rate RunN " " S/N% qd SCAN 3 SMED
SMLt SMPL
SPAC M SPAC q| SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE G11 Tmpr < User phAR phCH q phDY big phQL c phTR phTR