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(,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRLW\]]]]\[YXWUTQONKHGDA><:7410,)&$!___UUUyyy&))455@@@KKKVVVaa`jjjttt}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c0bd06bd839f4c2399518784145ee96bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-27_00-59_05893130Seq1231012310_Costion_K1364_matK_Fjmadera96-WellK1364_B9KB_3730_POP7_BDTv3.mobSeqPop7z !7J[l.Ic|2LfsEtL"xX< sh_XPH?6.'#"#%''&#  ! &.6>FNWcr%6FS^egd\N=(}l^SI@82.,,,,+(%"!!#%')+-/12222358:<=>@FP^n#aJN?szQb'{ia_aa\O;}Q%xh_YVRMHDBCEILNOOMLJJJKLLJHFDDHNYh{/cY!7_Ew '!L6fTIDEKWk5T#eo%hsK3&!#'+-,)$!"!      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""%"1"?"L"X"e"r"~""""""""""####*#6#C#O#\#h#t###########$ $$"$.$:$G$S$a$m${$$$$$$$$$$$% %%%*%5%?%J%X%d%o%z%%%%%%%%%%&&&&(&5&@&L&W&c&n&&&&&&&&&&'' ''*';'M'Y'''''''(('(2(N(a(s((((((() ))6)O)n)))))**  # '.%)$4:6::: )>+/)>$$'>>>>>>>>,$>;;;>>>;;>>>>>>>>;'>>>>>>>>;>>>>;8>>>>;>>>>>>>>>>;>>>616>>>>>>;;>>66>>>>>>>>>>;>;>>>>>>>>>>>;>>>>>>>>>>>>>>;>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>;>>*>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>0>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>111>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>>>>>>>>>>8>>>>>>1>>>>>>>>>>>>>>>>>88888.>8>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>'/>/%>>>>>>>>>>>>>>>>>>6 >>>>>>>>>>>>60>>>>>>>>>>>>>>>>>>>>>>>>>::::0:!::::::6:::::::6:::::4:6:::::4::::.::6::::6::6:::5":3:::5::3641:!:::::1:13):::1:,:6355:::2:,3521,,!22::163::::4::3666646646551666344-66(34(6#-..6 '#3/..343 5)** 3)/" 5-'3*3333+*.+--*+3-)"./*+*0.&*+++&$*$!)0.0000/../+.,) $*&/,&   0       # '.%)$4:6::: )>+/)>$$'>>>>>>>>,$>;;;>>>;;>>>>>>>>;'>>>>>>>>;>>>>;8>>>>;>>>>>>>>>>;>>>616>>>>>>;;>>66>>>>>>>>>>;>;>>>>>>>>>>>;>>>>>>>>>>>>>>;>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>;>>*>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>0>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>111>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>>>>>>>>>>8>>>>>>1>>>>>>>>>>>>>>>>>88888.>8>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>'/>/%>>>>>>>>>>>>>>>>>>6 >>>>>>>>>>>>60>>>>>>>>>>>>>>>>>>>>>>>>>::::0:!::::::6:::::::6:::::4:6:::::4::::.::6::::6::6:::5":3:::5::3641:!:::::1:13):::1:,:6355:::2:,3521,,!22::163::::4::3666646646551666344-66(34(6#-..6 '#3/..343 5)** 3)/" 5-'3*3333+*.+--*+3-)"./*+*0.&*+++&$*$!)0.0000/../+.,) $*&/,&   0     PbLb1GIGY+I_O,&N>-d:U!B&+a%bT*V(6#A/R-cQ`:CP;M`(?%"#/HMID4>9@`6$-@B']+7=7')c+9\#\G;U=9b&IVOU^3IA #8\V'_ #'.J+^X>V&R(OS=!KI7Y1%X[LA" 8K >'CDXP<P *F3;`3SZ.@= dO/(d><G"&%Y "2#RR%P"S0` 6B63 @4$C]Z-6J % AD(F,Z>:"8HZQCS?<+257\G,HN"5L L^C.dH`)07,3'XI *=\CDDQ# ,)?OU/PF@E)L5R 0U^QAcJ` ^B:b(HMJ,Z*JW\'_/RNFA 0*MJN_ O=.L4/=7=E],_ /RO#]K(#-]4\X0+5d"<+LbQ-<$3 V*`!1$$_, -CSD-L)!KLcKLE` Q G5GR7)#8CYP/MO_TI\!S*3(B"$*:\ V7#D GC2<9FBZ K&B CKF$7\ 1YB<3D$I?JU3\L4Hc#K >J4d*E@,,[&1=2Z^K[/ TD?8ST1_WM&bO4^<^01KCZE<]7%%7@<RT/[W >I'C[:=+MN^d aH&"JF $-BRE?`U<$=/,-,BHE172-(QDVXTS)&<2(.7U2P,!^-2bJ": WW1HUJ^RE8,]P.8a222(CFP"2 a[+H@2EP%?422a+",%>2<Ub.C $0#CPHb )=^RTC (AM,OR#c` v.E;J6^/0>lu-2MY3cHSZ1 :3c%>UL4U2s##Xfh^S>ws8kCBc-wy17,^&&U5.d ICB6Z`4(1oYbzfdm7$ YsH  -`7u2\yOb(,bh _6da7 *]'`8 &2JGRH#8 t.(n.fZz!~yTdqX,U& ^2.7|x6/X bR_~e522D|LDZ_u/eL}V:MP#K(q6fhDu:ziLPS.UuJ?K*CCL%x"1PC%;Er&Vc8'dv7XV  s"CsWhjf9B&*15"_PG=ji2jA z[UHr k.;:U(q4 2O]L[3xr'`$E2!w &Ob\:g }n|eKtg} R:)>v(`V>3"Sb H?t  "" HPbj) +Hwor6ln`@WH@@h@F>F2)2D998+2xE,3""$  &"    *          #  0  #  "&#'"& %# %&$#$!+,',)*+)!9.+/6'0-1%:'--.*+4>3#-<890789>B74K23ESF8L:<G;J<DC>>GQODAJNC]CHO8NPoYg~YEL9DUUUUUpqM?U]NsYGW<M[\cV`Nmy_wll_Smi[lhhhrgn[]hrl|yk|rq8m~zn9"ga U%Z[a[}Z[aaaaa[a[}[}a}}[[[[}[[ZtZ@ J#'a;xod*\ap|j8Zp2py*py'@7O,K5jC`}|'MdU{]pLn"{ D=86>Ai$`yA!dLpa$.x~/A dG8[KdR?8[6il[$yov`9d]ddx?8pY,Ox+}dOQRd(8/,Qp,>,pDse{CR!To8,.l9t84GnpQGeE76<*QvdCp ,:dB,Pp$ Bjp,(pp G=gvppp+ppNpYpoppp80@{d;NhtAmU4+@bO ];[ O-~yItH7[a5Dj9Sol7;DF=k,I:PD'/'%*d3D)4 _ ,+R!,*"*-2/:j  *#6*"  !      /$&! % (& ' *  '$ *%! %  $*' %     !     "! %+# )  )  eE*  ""  =%  9/0.#r ! -'" <,3)+/"!!,)" = 6,0C!"*<>((!]$/!"*V5!$ ~L!b E>g-#'39#m4g<(?*!8G0O["7?5Z7=AAV5IB3B;+BI4}arBE/Kn)LU6;*}.l]\XLITrU2nhHinMxbJQ|B~;UxuW}cM  !z8gpeHF`q4^1m6\>     GGAAGTCTCTCTAAGTATAGAATACGCCTTCTCCGCACAAATAATGATACTCCCTCTACCAGAGCGACCTGAACAATATTGTCATAATTCCACCGAAACCGAGGGATTCTCCTCCTCAAAAACCGGGAGGTAACAGAGGCTACTCCTCCTTCTATACGCCTCATGAAGAAAAGTATAAGTATCTTTCCTTCTCAATCAGCAGGATCTATCCTCTCAAATAAGAACTATATCCTCCAGAGGTCAGAGAGGGAGTATATACCTACAAAACGTTATAGAACATCGCACAGGAGTCAGCGAGAACTCCCCTCGAGAGTACCGGTTGAGAGCGGGAGACTCCCTTGAGGCATGTCCGAAGTATCGAAGGTTATTTACTCCTGCCACGGGAAAGGACCCTCCAAATAAGTATGGAAAGGTATCACACCACGACCAACTGTTTTTATAATAAAAACAGCCGCACGCACCAAGAAGGAATAAATATAGGGAACGAATATTCCATCCCTTTCAAGAAACTCTAAGATAGCGAACGAGAGAGCCGATTGAGTCAACTAAAGCAAGCTGTCCTAATGTAGTGTATTTCAAAATTAGCTATTATTATAAATATGAGAGATAATGTAGTGCTACTAATACGTCTGTTGGTATAACAGTCGATGTATAGTTAACGTGTCACACAGTCACGTCTTCTAGTTATGACGATAAGTCCGGCTAATAACATACTCATATAATAGAGAGCGCGGTCGGCATATATACAGTTCTCACTCATAATAACAGAGTATATCCTCGGGGGGGGGGGTGCGTGTATCGTATGTTATATACACTGATCCCCTCGTAAGGTTAGAAGAAAACCAGCGGGAATTGTATGATGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-27 02:31:25 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSxBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *ATDATA *AT9MDATA *ATDATA *ATQDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEMLAST~~wLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS6P1AM_XP1RL_RP1WD__P2AM_lP2BA__sP2RL_f@PBAS__:mPBAS__7PCON__NPCON__KHPDMFPDMF6PLOC_DPLOC_=PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;,RUNT RUNT ;RUNT &Rate RunN""S/N%6SCAN3SMED SMLtSMPL SPACA\eSPAC6SPACA\eSVER4.0SVER xSVER,,Scal@Scan3TUBEB9Tmpr<UserphARphCH7 phDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSxBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *ATDATA *AT9MDATA *ATDATA *ATQDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEMLAST~~wLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS6P1AM_XP1RL_RP1WD__P2AM_lP2BA__sP2RL_f@PBAS__:mPBAS__7PCON__NPCON__KHPDMFPDMF6PLOC_DPLOC_=PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;,RUNT RUNT ;RUNT &Rate RunN""S/N%6SCAN3SMED SMLtSMPL SPACA\eSPAC6SPACA\eSVER4.0SVER xSVER,,Scal@Scan3TUBEB9Tmpr<UserphARphCH7 phDYbigphQLcphTRphTR,9drWdG| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz99#memI+pehRIi}'+-HHD5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! K]T5^.?s|"Ff61}XTu.ycI0 r#U438n,E:F`+DhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#/D?*Uq$[-o9D4`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k'eaK|`Bt#2ze/z,(,y]18`F7IOo_3@/C 4O$AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDT``B1PtB1PtBCTSxBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA *ATDATA *AT9MDATA *ATDATA *ATQDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEMLAST~~wLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS6P1AM_XP1RL_RP1WD__P2AM_lP2BA__sP2RL_f@PBAS__:mPBAS__7PCON__NPCON__KHPDMFPDMF6PLOC_DPLOC_=PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;,RUNT RUNT ;RUNT &Rate RunN""S/N%6SCAN3SMED SMLtSMPL SPACA\eSPAC6SPACA\eSVER4.0SVER xSVER,,Scal@Scan3TUBEB9Tmpr<UserphARphCH7 phDYbigphQLcphTRphTR