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{ y x l | ~ } { x a x } q Y i ` k v } | s n x u g } b s Z e W L R N K F S ^ W S D I 4 > G / > 6 Q 1 @ D R M [ ] c T T M K = A 9 C @ 5 ; H < F / * ; 1 & ? + < 2 ) ; 1 - 5 - + B M T B P M R K Q N Y a x ^ K e b V i l f ] a b n t '; ;)D $, { x | ~ q g s q { x l e j t R ^ T W a X M B J B ; G 9 ? > Q a a m i s x t m p q \ P H U _ H K M R H G E A D 0 L R X J J U [ \ d p k X ^ h i ` m n k Y S T J J Y v X b b m q w \ a E e } @( l e U N ] [ Z D 1 ; Q = ^ D I C A ? K C B I H H X > Q V Z W a \ P ; @ C 9 > E ; A - , 9 9 F 5 C C > = H 3 @ I > M S : V U O N I W S Z p } } t O H ? G ? A B & @ + 6 : 3 9 : B H T F e z s s q V b U [ D U X b e a ] q d f Q V G ` r { j ] Z T \ _ Q X V R E r Z J U F U R ^ e z w m v s b V _ b S I I c ] r ` s r p | n j X O L M H W B < 5 A X L \ v x } s t f \ T R ` J M D J X A I 7 S S : X @ L W S O E E P G V g V [ g Z f _ ^ O U S Q S S J Q G I I [ L X [ C V i k ` v s { u i k Z h ` b i o W _ T a @ = C B I F J G E B ^ d c f l y y p r f y q v l a v b r m b c l ^ a i \ Z h i a o z V i n h a d Q Q X Q N ] c j ^ v o m m c k c f W b H M O : V R ? 8 M = ? < F 9 ? C 0 4 B 3 B H 9 ) / > > A < : 4 2 = > < / 7 C I T A R U ; B : L 8 ; 4 7 5 / 4 4 @ I I M M Y T V \ l M X L : @ G B 1 8 - A 7 < @ - - - 8 9 & + / 7 5 ' ( 2 0 1 , 8 & 8 = 5 L J ^ V t H Y ? C D = = 1 < / 3 9 > % 1 J 8 S 8 : * 1 E F 4 9 . B & E G b l k Z h q q k ` Y b S b e ^ Z V U N Q 9 1 P ; Y b o y z z p E P S Q E : > > K : - & 6 * 1 4 * : ; 0 6 2 , 3 4 ; & > / , 4 8 5 I A S V U V a e d O = ; = , , 0 6 ) 6 + < [ H I J R D ; A 4 / 2 Q O T a j a q r l ` X ] k h [ C ; : I G 9 < : F I 8 ; C , 8 ? C / # 6 4 + ( > ; B N M b r z o [ [ K J W M D A 4 , - 9 4 7 M Z e z g u d _ l t h c k c k k { s y z ~ ~ n v t \ i d h g d m p n | r w o c d ] r a v r p y r o ` { j i | | f i m f c ` ] Y a ` \ M A = 5 @ I : @ C 6 : 5 : ) E P R Z v t l j f e a Y b _ } k l | r x h f [ d l N E H J 7 / B 8 @ < V B M E T N K F < N : ? E E C E & C 9 H > K E E A A D M T C r q v | j s f u b P T M 6 ? B L I H ; = ; F W G T a Y F = - : V K ? T H L c h N S F M H > I H A E P g e a c W b Z O [ V h m r ~ y p z f Z b l | m v h U X a J N ? J K C C > E / = : U a y s c \ S S C > 3 O A S E K F @ D G C / 0 : 3 : & @ ' / 2 . . 6 ( 6 2 ; 7 + , - , + ) > ) 5 . 5 ; < N A 3 A H I > : 8 6 3 8 ( ) ' & > - ' / 1 , ' * # , * 5 & 9 : 5 K L [ L o K N H L B - = C J W ` T H M N J J / - 9 - ; 0 7 ) + / 6 4 & : 0 7 1 1 0 2 & 5 2 # ) 9 + 2 @ 4 0 = 6 A ; 2 C 1 . 6 5 . % % 6 * ' ! 8 - 0 & = B ; 7 A @ B @ & 9 8 H , 4 $ 0 ( ) + 6 * 5 2 5 8 9 A < J H O P ^ W V R G E H Z V i l t s s s { r P C L 7 M Z > I @ 3 F T : E W ] W } r ~ | t | c } ^ ^ U J X b v w z x x { t } c t ] | g V e ^ P ] e b p m w v ~ | h i f L A J ? R e S = D < Q > D I S W ^ i q v ~ o i ] X a J Q @ I 8 E D ? - C H 6 N @ E = L 1 5 B > 9 < - 3 9 % M : = ; F L U b h r z h ~ U [ N A O T ] Z \ L T H 6 > > A < 5 3 > . L H < D 0 . 3 Q U K W \ R g g k h ] R T P U d b z w j l r w o r r p e \ [ J T u ^ ^ Y \ x w [ d d h t j j | s ~ w | w a t n ^ V S X W C \ Z j w x { ~ z x W l n e c ` ] ^ m _ T Q i _ N ] p x z | z t v t \ k q m e } s z r j k O N _ X h h a v [ \ G H B F E 9 = M C M S K e V k ^ a g ` Z Q R ` d k U m ` m c r ^ V ` B > X ; = Q U B = ? D = - ? 9 ; = + 2 : + 8 F ' 2 0 , 4 . ( 2 + 4 * , / # + 6 0 ' 0 0 C 2 ; . 4 & ; 3 ' 0 * " & : > 4 G 4 7 : E 4 @ T H @ : H S : ? D < L 5 @ = E 1 3 : H 9 = C G A F 5 B < K ? C G E 1 = 4 J < 4 8 : @ = ? H W e c t y x q p u p x q a b _ E R \ F M = Q E O O M Y g d U I K H g W Q F ^ L ^ 8 C H 6 N C ^ I L O ` ] g u ` e i d f ] S V O B K M A O T A g [ O m x g s p | ~ b l V q w x | V g o M W O Q U C T ^ Y } m f r b X ] X F O [ S R P R G \ T K 8 ; 2 3 1 , / 5 & A = - - 4 % + 9 9 3 1 / " - , ? 2 E 4 B = . @ 4 0 1 8 % & & " ' ( ' 9 # + 5 8 # 6 8 B 7 A C S R H X \ L < D 9 4 8 E 5 J O 9 ? ; : 1 L G 3 = : 8 < A 8 7 5 V Z K ` n j g o v g ~ | b b g V J A R I T ] ` ] q x u e i j X ^ \ X a c b ` U Y _ U W N ] T Q P S ` j U o o o V a O G > G E P Q 9 > H 1 , @ 4 4 2 A 7 $ 4 % * < & + + 4 7 % % " % 7 % . < " < 7 ; . F : 0 G G < 7 * : < < ? ? 6 & - / Q : 7 T p p m d [ J : : 3 7 = & - 9 " & ( & ' , , # , 3 7 B , / / , % 7 B 8 , D , F 9 , # - A 1 / J O [ j | S O U N 0 5 > , : ( / ' + ! < G < Q ] s o g P = B " 5 1 ( $ . ( ' * $ 7 9 ; @ C V { u | s w ^ W ` J b J F A 9 6 - 8 ) , * ) ) & ; > 0 , 1 * ) , ! 7 5 - 3 > 6 ( ) 1 ' & * - $ - , & . ! - / + - & * , " ! ) 4 + & * % $ # 4 " - * $ < * 7 ( ( P * 0 & ' . * ( ! % *
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a2934fb359d1475c9c9d95523e1f2f09Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_23-29_0588 3130Seq 12309 12309_Costion_K1363_matK_R jmadera 96-Well
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q ~d JD ), (ZqVz`g ?P05 mc9M o Y2kDC _ D gL`Cy:Ov q Dic Ic ; ' @ AGAATTATAACAAAAAGATAGAAAATTTCTATGTGGGTGAACCGGGTACCCCTTCCTGTTCACTCCCACATACCCTTTGAAACAACAACCACACCGGGCCGACCAACGCCCCCTGCTCGAAGCCCAGTCCCACAACATTTCTGCACCTTGCCTTCGTGGACCACCCATTCTCAGCTGTTCCGGGACAGATGACCCCCATCACATGGCCCCCTACTAATTCCAAGGGTGCTCCCCGGTTACAATTCCTTCTTCTTGCCGGAGTATTTATTCCCGGCAATGACCCCGTACCCCTTAAGGAGGGAGGCGGAATGAGGACATAGCTTGCGCTTGCATCCCCGGGCTCGACCACCAACGTGGCCATACCAATCGCCAGATCCGGGATGATCGATTTCCCTCTTTTTTCCACAAACTCCTGCCCTCTTTAAGAGCCTTTTCCACCCCTCCTCTATTTTCCTAATTACAGAGGGACCGGACATTTCGCGGACCCTCCTAAGAACTGATAAACATTACCACAAAATCCATTATCATTCCCACTGACTCCAAAGGGCCCTCCCCCATTAAAATAACCCAAGAGGGTTAATGTCCATAAGCACGTTAAATTCTTCCGAGGAAAAAGGCCTCCACCCTGATGCACCACACACTAAGTGAATCTATGGGGTAGACATGCTCCCGTGTATTCAATACAGTATATGGAGCATTCGGTCTTCCAAATCAGCTCCCTATTACCTACCCTAAAGATAAGGAGTCCTACCGCGCTCTTAGGAGTTCCTCATACCGACCGCTTTCACTGAAACCTCCCCAATCTCCCACAGGAGCTGTCATATGTGTGGTATTCCGTACCAAATAATCACTTAGATAATAATTTTCTGCCCACTATAGTTTATTCTTTATGAGGCAGGCAAATGAGTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-27 00:59:49 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt d ASPt d B1Pt c B1Pt c BCTS , BufT ! B $ CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +, VX DATA
+, VX : DATA +, VX A DATA +, VX DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM a P1RL Z P1WD h P2AM v P2BA ~ P2RL o PBAS A PBAS >, PCON W PCON Sz PDMF q PDMF > PLOC L` PLOC EF PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
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RUNT RUNT :; RUNT 36 RUNT :! Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL f SPAC AXm SPAC > SPAC AXm SVER 4.0 SVER ! SVER , , f Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH >' phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt d ASPt d B1Pt c B1Pt c BCTS , BufT ! B $ CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +, VX DATA
+, VX : DATA +, VX A DATA +, VX DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc v FWO_ GATC Feat / / G GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM a P1RL Z P1WD h P2AM v P2BA ~ P2RL o PBAS A PBAS >, PCON W PCON Sz PDMF q PDMF > PLOC L` PLOC EF PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT RUNT :; RUNT 36 RUNT :! Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL f SPAC AXm SPAC > SPAC AXm SVER 4.0 SVER ! SVER , , f Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH >' phDY big phQL c phTR phTR eZ>\&bD8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|$q_{U44bK&Z[JUz._Z5TVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*R0 L#153~l?A_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_>B|::zWT|O\rwHB*@Y%H_TQFGa^w^8/H AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt d ASPt d AUDT d d B1Pt c B1Pt c BCTS , BufT ! B $ CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA +, VX DATA
+, VX : DATA +, VX A DATA +, VX DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc v FWO_ GATC Feat / / G GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS = P1AM a P1RL Z P1WD h P2AM v P2BA ~ P2RL o PBAS A PBAS >, PCON W PCON Sz PDMF q PDMF > PLOC L` PLOC EF PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
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RUNT RUNT :; RUNT 36 RUNT :! Rate RunN " " S/N% = SCAN 3 SMED
SMLt SMPL f SPAC AXm SPAC > SPAC AXm SVER 4.0 SVER ! SVER , , f Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH >' phDY big phQL c phTR phTR