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R Q T G \ R D J U T K P _ ` O X X S ^ P j l d P [ d c f X a a ` b l c d d X Y b ] P U X f Y G ] N P O ` L Y T E a E U S V E \ W N N H ] G W S N ^ Q P W R ] V J = 3 V X K J B N 9 H e \ b K N e T Q I L G L C J @ I ? S S C G H B S V O Q B O H O a \ c a p X b N S Y V G I Q S P S G J ? L F N = N U D G O R = V ] J F R O K ] R W ] q r } n n i U h L T d X O W ^ k ] t Y Y j j b a ^ _ d T Y R S [ L L L S R Z Q Z U W T J V K L C J A S A O X [ U P N J D < G D R X \ ] Y T T Y V Z Q Q _ F ^ M H T K S Q H I X U a _ V [ R W [ N V Q T O G _ ] [ M [ _ Z S Z H b b ] ^ a i ^ Z Y T T K S ` K Q [ M _ Q K Z R H P L O Q N I G S Z N L [ J d S ` l \ k ~ g e ` V Q ] Q F F I N L Q T \ p i Y p j ^ e O T W J M a U ] Z ` R M Q I D < M Q V K W U Y R 6 H A V _ U J X V J H V U Q O N ^ W X ^ a e l e k a d X ^ N Q \ ] \ \ V d [ ] k _ \ d o j b S Z M S Q K I K O M K W Q N O b Z e Y u o a e [ [ P V N R S _ S K V W V ^ W Y Q H N Q ] : \ L N J I Q _ n \ V [ L X i [ T i e Y L J ] a a ] J @ Y P ` c ] T [ \ N a h Y V ] i f g x g e h m ` a ` e g e h x k w i s s n h g m X o V k U w l i c m c ] u u h l g h j l k c o Z P _ \ \ W [ g b M O Z U b h _ g Z m _ ] S \ \ Q N C J R H Z \ M P M N < Y ] f ] i ^ ` Y Z k j ] W p [ k Y P Y a \ e t l y _ g d _ _ T \ G V Q \ L b H a \ Q T Y F J H j X \ R R Z Q ? M O K K R j V L _ Q Z T b ] l ` ^ j o i t t o p h V q j k f b W d o a c c P f W ] S a V j g j c e [ i W O [ b Y [ T O F H X G P J Z M O ^ N Q ` O _ O I i G V Z U C I K V b \ R Y J G C H H L I H b b K F Q V E L U P K L P P Z U I Y _ P Q M \ C ` [ Z Z W J [ ^ m a ^ Y a \ Q Y _ U a g Y f R i [ h ] Y j c b g ^ b c e R b ` g [ b R P P X S V G [ J u _ W T ` m o o q d c ` b P c b a K ^ K Y [ V T Q T _ P ] P c Z K X K P I M G X C N S ^ S R ] M E H R [ ? ` Q M L R P P O Y T Z S W N J c b b Z X Z \ H X L b ] X Q [ e [ W U e [ M [ b C e b i P v m i p z y e l f b b T ` c ] i d t j w v i j i h s r W [ p [ ` ` Y b X g X k O X Q W X W I a F < B Q Q L J ; N 5 U I K R I V I K Z F Y K i H B L i _ b f g h k ` \ W N M _ U U V a S k S V T ] W J b h T E N P A O i I N O Z K W [ c Q O H I P K X G W U U \ _ h c d x p p g a n b X g _ Z K H M S I X X F ] N T Z W Q b ^ W K L C X 9 T J R P M a P ? Q Z F I ` L \ R @ O L _ O a O X ` \ b f Y R _ q Y m P W c a Y H Q V U Z M Y F C G P P G O S J I L J J N I H P M > I F L P Q U J W E A I H K K T I ` Z T W [ E L J e P E I V J P D Q J P A F R B T 7 C P V P A > O K L : 8 D G K T L F M A U U Y L 7 Q F H I U I J ? I P R K J E K X ; N M G N M A E 8 I @ G : O E V S L H P Q L X _ ^ Z j b T a Z Z [ \ _ [ Q \ U \ H O F R Q R A K O Q U O E I X T X C O H 3 H 5 ? 4 ? D A D F U B E F Q C A P F : L I = C O H I F H J M O I K ? P I R C [ J W G F O S V D K W E @ H U A Y E M N ` K ` J 9 O B J L > J @ F E E G @ D = Y X J O P T a N @ X Y P E U M O L P G S ] W Y n b \ o } l Z S i U S J R G K D < C @ P B U J Q K \ \ h j S U a U @ S G R N T L P M F U U J B _ K S 8 O M D J ; C L K F W E G @ I > 9 ` \ ^ a a ` P O M Q J R F Y C J T O H G G U = S D J U T S T T g j ` \ i Q R [ V X Y Q C G B V P R ] X W [ ^ X c W [ \ _ d ^ a e \ W [ N S Y L Z D Q L D > U I N H G I Z M N U S Q T O a \ J I X I h _ Y e ] o j T W { Y U [ O T X V R J Z K V V e Y Y P W Q O T F J S ] U K P V > < M L B K G 5 B O < R W K @ P W E X R E D O I Q S O L K P ` < a R A J F I @ = B S M F ` [ R _ T ; N M H B N A L 1 M Y L Z D : H > X ; N M U K Y Q K Y P K K R ^ Z Q Z ] W p g b n d h U ^ S H J N L Y Q S ^ k j ` _ Y W Q H B \ Y O X T G K L R A O Q J H P 6 Q C 5 N 7 M H O U D G G I X Z T > P H Y N O J K G L E R Z L d _ h o \ P W a T \ V a D \ D N < M 0 E P ; V D L H 3 P R K H G B P P ` : ? F P C J K L K R H G L L N Y G U G S E I H A D I F E E M T J S R R V N M J H 9 E B J E H 2 F > = P < K A T 9 B . K K B M N I @ M A : 8 O H D G J E G B 5 C Q = R S @ S F N C A E E D C G E F 2 6 K A N S ; I > Q E ; J C H J = @ W > , I A 5 Y @ H ; F ? B I A L G Q : L C G 8 A C C C K ? 7 @ D B 4 H Q B : J = 4 3 C - 7 & B 6 ) ? < @ 1 6 A N < 5 F B = A 1 A > @ 3 B ; = J D C H = F F 7 D K U M F H R J L C M K K = @ E @ E = T @ C @ I ? B : E = 8 ? D ? 7 H = : 2 5 @ 9 : C M D 9 2 T ; 8 5 C F E - J 4 5 / 8 : @ G > F @ 7 L ; ? ; 7 8 < A C @ E B L S H M J F G J H L A A A ; < 7 P 9 @ C M N E O D 4 9 E D 8 F 9 > A B D @ M > < : A S > F I F D R Q Q V _ U R T P E K T H > K N O F A ; C Y X L D M U N 8 ? @ J C H B = G I H D > D B F B D K 5 C @ = A A 9 C < 6 B 0 8 = A < ; ; C G 5 9 7 4 3 E ; H @ ? I < E F 1 U E E F B F L I I O 9 B R L B N S W W M S O > T R I < 3 > < P 4 6 = D C ? ? M @ > - D J L G 7 @ D G 3 ? B J @ @ ; > < A E 6 8 H : H A < < 5 ; * J > 7 A ; 3 H E C L A C W D F L S M M S B X Z P S 3 H A 7 ? ; 9 : ? ; A C E = O = I 2 9 2 H F D I 9 I ( A J F J < L D 4 = E E 7 I H > ; D F L F G I E P T D L F A B = H / 5 4 ; J 6 H ; P 6 5 ? ; ? B ; B 9 I 0 - < 1 F 3 L ? C P G N W A L K F U E N K H F V b O 9 V D Q < A S B D ? I F = S 5 G I J V O ] P : A K H O F R F B U L J G C A P T < = B . > I 4 K 3 . F I N I Q F ? R A G L = H = > U : ? 3 > 0 + F E K ? : < < . < ; I F A > 5 H L [ C 3 > . ; . ? 2 A B > = F * = @ ? ; 8 D ' C ? H 3 6 @ H D G E H N F = B C M C R H I R W X ] P U Y ` S J H Q S Q F ] W Z P Y b T I K @ G A < @ 1 F J @ = J Z G ? G P > @ I F K D F J > J R T 8 9 O I A > F 7 @ 5 H ? ; = > 7 B ? = < . 3 > 3 E ? 7 + 4 8 : $ - . 9 ? % + G / / * , . < 1 = : 4 E 2 : > 5 = / 3 E = 5 2 > 5 7 9 3 9 A E V M < C = B M 9 U 1 ? A J C O . F I [ I T k b c \ W K X V V : E N @ 7 2 8 F 3 6 8 6 B = = < * 2 < 7 ? - 9 5 : J G P G J M U A L B G P ] C R N N W \ P V L P S ] R X H A K I C M : 6 D < C 6 ; : / C ? = > 8 : 6 @ . : < B 4 5 ) = 2 8 @ E S J M B 6 M R < < F 5 7 + 0 / C 3 : , 8 Y = G ? = C > B 9 : ? = B : D 4 1 , ( 3 > ; C 8 6 , = 4 4 ; 4 < 2 . 6 3 > 8 < 6 7 7 ) ; 8 = ( : 6 & A , A , 4 3 < ; 9 F ? Q : ; 4 H I J F D < 9 O : 3 E @ 8 ; + 4 0 = 1 G F 3 O B H 9 < I / M = N @ L Q a L N 5 @ K L L : 2 + 2 L 9 . 6 8 A G 4 9 ( 8 1 / 4 7 2 : < 2 ' 4 $ 5 5 = 5 8 0 ( 5 1 2 @ 9 * @ 5 2 B @ * . 2 $ ( 0 7 + % 5 : 8 : : G F 9 < 8 2 ? 8 A * + 9 F : R C A < ; > > D 7 8 @ @ I > S H R P N P K 3 @ < H ; > D J [ F S 9 C 8 = I L < 6 5 9 B I F 7 9 3 < 1 I 2 N 6 3 E P 0 & B A 3 > I P @ ; @ ? E ; F < < = 9 L + ? ' + 3 + 0 / 3 @ 8 $ 1 : E > - = ( ( 9 @ 6 @ 6 7 ( ) 6 / 0 3 1 6 & 3 1 5 H F 2 4 I ? D L P M @ F a T H V R F P R I ` X Q ` b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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2c067e5c9b5643adb74b75c0f0e6c18dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_21-57_0587 3130Seq 12308 12308_Costion_K1363_matK_F jmadera 96-Well K1363_F9KB_3730_POP7_BDTv3.mobSeqPop7z 3 ; F Q Z \ T G > A R m 0CMPNJHE?5'"6ITQC.
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V*TQL@ :W4Ho>UwB. P^iS*[CAATTAGCCCATGTGTTTTTAAGAATTTCTTCCCTTCAAAATAAGGTTCCCCCTAGATATAAGTCGCCCCTGAAGACCCTCTTCTACGATTCCGAATAAACCACCCCTTCTTAATCCACCCTACACACAAAATACTTCCACATCCTCCTACTTTAACTTTATCTACTCTCATCGTGCCTTCTCCATCCCATAAAAACATCTGGCAATACTACCAACTGGTATCGCTGAAACTTGTAGCTAACCAATCTCATTCCGAGTTCAAGTCTCAAGCTAGACAATTACTCTAGGCCTTTTCAAAAATCCTCGTCCTTCTAAACCATCATATAAAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-26 23:29:29 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #` F DATA
#` F + DATA #` F rK DATA #` F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE E LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM K P1RL K
^ P1WD K P2AM K P2BA K K L P2RL K PBAS K K Q PBAS K K PCON K K PCON K K PDMF PDMF PLOC K 2 PLOC K PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9; RUNT RUNT RUNT 6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F9 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA #` F DATA
#` F + DATA #` F rK DATA #` F DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab (z FVoc (i FWO_ GATC Feat . . (; GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE E LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS P1AM K P1RL K
^ P1WD K P2AM K P2BA K K L P2RL K PBAS K K Q PBAS K K PCON K K PCON K K PDMF PDMF PLOC K 2 PLOC K PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9; RUNT RUNT RUNT 6 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE F9 Tmpr < User phAR phCH phDY big phQL c phTR phTR "3Z2VW)>ZHm? V<(?:134]3[16Qu5r`xC>2aZ!ly^A9Jz?gMBI+\$jn18A7y!H#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;{v3Ah-8^#~C=O\rHU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nX>zt %v1?{6W8:7F}wxSB#_{=pQ$+Sn0&'*#))MR]N=]TAO$Cs-1G@cJ-omMOxq6agI)Y_bO?C!.tk,8#V>Ax7cXF$hHz))OH"vln>(Q.2|x>3E S>|{^;5GKXW .C=vz^`ZeW\jhA?I| 9I+IaH]#[,e/G,y"xc_M@a7&^LoT.iFZ8EDlyj,K]yDWYlsDpN$t.D$mOYePjG,F<_ }OuPzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=K}2?Yk".Om,4oFo;X.pHwT_v^G*Pm(8gOKd]B4Rh#*vWcM=Jg$-KqN^Fu*te9r=u4TYe0+Vsp@O3sT6#f 6Y?03t0mP@*26G !9W&a:Ocdmo-JmE;9xxsWD@TZmU)P? sz{fzVSA]!lZbCd]'n,J6v`ZdD\4A,h{|dpwM5rm;p=_A26O1HnYDx8_b_l4P3LFRt( g>::#Qx80OnmonH?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*