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T 8 I A B 1 4 D F 6 9 0 6 K K 1 H D 9 7 : @ E L I a \ F T O N [ I Z \ 6 ? D A P \ F _ Y N U U H E P G ? K B E I W Q O S H N K : B ; < 8 : N B 5 G A _ S E O M T 8 7 P [ W c ^ f S \ Q G m Z a \ [ t V d i S K O Y M N Y V M i Z _ X ` f T M L W V G D R Q P L f V T P G Y Q ] [ O ] K Q J O < U V Y V I Z f X [ Z \ T [ N I R F T I R . : H ; L K > C J P V m s g r q e f g | X p g _ U M K J I U f U X k T U S ] J J L H > P Q X J O H O G \ U H ] N K U N H N c Q I L @ L @ A C P B E A F L B D G K < K S G A G L N ; A 9 > C ; # 6 > A I L > E K M M O ; M ^ L P L c i T j R K W H B ; S N O M c a d \ ` [ P ] _ U M P Y \ ^ h h W ^ _ Z 8 Z I O S I P [ U O E k Q K D ] M R > D F E J L P \ J H V W f c [ p n W n h \ Z V g [ c s v p { y u v s ^ ^ q ] ` l s v u c o x i u u s b g g O h k \ _ f _ O [ X [ [ S [ X d \ C ] N Q P U V V a _ ` a S I I > E R ? C T J L @ V F S F F D P < ? = ( 3 D B 4 I 2 8 C = O O > 7 E 0 ? > 7 L 7 I 2 3 ; > E I 9 8 ; F G = 3 @ 9 ? ? = 5 5 3 @ P F 4 B M B A E = < @ < @ ? 1 ? > / . = 3 : : @ < . 6 A = 3 - @ , A B B V G K = I > D 6 = L 4 K 8 D @ J ? 9 4 < 2 = 7 7 : @ 6 ; = : 1 * 9 7 2 4 D = 1 ? @ 8 ; , 7 . , 9 I 8 < ) , 5 1 6 C 5 - 3 - - C 6 9 3 > J : 4 L R F G 4 B , T = C ' 8 < 9 4 , C , A C H & 2 A 0 6 . 5 ) < 6 = - . 9 ) E 2 7 @ : 2 2 6 0 5 5 . 6 5 , - = 7 4 J ( 6 9 8 3 > @ 1 M 7 3 & K = 2 ) < E / . 3 ; B = O 8 B 4 ; 1 ; 2 > ? ; , 4 0 @ / ' C ' ? 0 > @ < @ ? + 2 ? ? , 4 A ? 0 6 = F : 4 9 , = A 3 6 ? 7 C I E < : ? > J / H B H C E F 8 1 B D L / B , 6 7 < - @ G @ 2 O ; < 3 ? B I L K A U > 8 I E > C I A O A V K E C G L Q . B A 6 / 3 = D , G > ; 4 C 7 B @ = F U T O Q E F ; ; = 6 : 7 F G H ? < 9 < , 3 ( 1 4 / 3 : - < < : > 4 1 9 9 < D J 5 1 9 A : 6 8 A 6 @ C ; : A R H < 8 I S J @ D H M 9 + ( H > : < 9 M : F @ A = A E < = L @ B G 3 > H 8 P F A V > C G E C K Q 8 U ; S I W P W U I P M D R H W > ; 6 : : O > - B D E W D > D < 6 O I 8 F O C 8 C B @ M > N T = D B K ; N N C Q D N J B C M E O B P M P L U J P P R D ? Q O Q h h a c t n k \ f d L H L > C F 0 K ? 5 ? U ? K < 8 J G > 5 V L 6 H J N O E W B P P L R c T T L W D [ S H K O B M 5 N J ? U D Z E 0 R ? H F X 4 I ^ M = ? K D N T A [ C J X < L A A I G : A B A G C C > = 6 L O < J C / 8 < D H @ O A D Q B N : E T I @ T U E U D < [ N K G O P M L O ] ] Y J < R J F 3 B Z P I a S b o n N \ U W J D < M P J J : L K 5 A C P < S I 5 E 9 9 > 9 C F , 7 . 6 > C @ 7 P K V i W ^ ] a e h m Z U R X V \ ] N g n ^ r Z j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` H R W P e K ^ f P b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i g ^ i d c M [ O N ` ^ o y n R ] _ ^ Y N V G S > I N 5 M Q 8 F E > @ < 9 A R P B B V G A K F D V Q O C G G Q R V C V C E E S K A G G L H B 8 < ? 1 F M N F t T U ] ] A Z G [ P D W M G L H P A R D A < N C = J < I ; A : ^ F H D J B G < T B K [ ^ _ e x z g d f \ K Q ? F N B D A 6 , 6 = 6 E 7 G < 5 / : R B K C D L 5 I G G @ Q T b f b n h Z Y Q L 9 = F > C ? C * 5 = = 1 3 ? . 7 F 0 . C F = : R L A + < 0 4 > B 2 / B ; 6 F 2 C ; A ) , = 3 1 > < 7 6 / : ; / D 7 F ? 5 L J = I D B 7 C + 7 = / ; D 9 4 7 > 9 6 O 7 C Q K = B G I ; B = B H D B ; > ] _ V X Z = @ I N J N 9 B E Q M O F L E K D F > < $ = * < 8 4 ; : C 6 @ - ( 9 8 5 1 2 = 1 ? 3 0 8 8 ? K 8 ; : 3 A F @ P V ` [ C V S 4 Q M [ Z U U j j I U d R W D F H U J K Q ` [ \ a S E F I ? L E J ; K X B E 3 I : L < H R Z \ J D G @ I Y 7 K M M J G M ` \ [ ] P P F O V Y Y T [ ` T D Y > I ? = : ; M Q J K G K E C M C F 8 ; \ A = I P 5 O 7 A T K ; Q < N 5 C B ' , ) ( ( , % * 0 0 / 4 4 1 C 0 5 B 8 @ = ( 8 7 E 3 3 G 7 = E R S X W T U T \ O F L I N N = S Q U f W I N T @ I G ; . B = 6 < C I 7 8 F 7 3 B = + H 3 ? > 6 ? L < 9 : 3 8 8 B , < I & H 8 A 3 = = : 5 D < C D A < I 4 + 4 0 0 @ - U , I H < < < D E G 9 A 7 F X E O Q I c O Q ] H N R I Z @ P > . E ? ; O @ ? B D > H N U N I T L M R K K G J G C @ N E : E : J 3 ; > @ 5 B C H : 9 B > F . > ' 5 5 < = D Z 7 8 A B 7 J T < : : A ? C S 5 K D < > > 1 < > 6 ? L R @ E Y C q ^ b a ] b S g Q O L Z P T O a b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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 15082239ff7c4d85a835d47a38fc2ad1Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_09-41_0581 3130Seq 12301 12301_Costion_K1362_matK_R jmadera 96-Well K1362_H12_RKB_3730_POP7_BDTv3.mobSeqPop7z '' 'Ch}ov(ZW!N}eD/+6Jc~.Jm{T4 &?d'7Kd4C8 {
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KB 1.4.1.82018-04-26 11:13:22 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS `p BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA * U` DATA
* U` :. DATA * U` DATA * U` DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ ^ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS :V P1AM M P1RL I P1WD Q P2AM Y) P2BA \ P2RL UU PBAS ] PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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4 RUNT Rate RunN " " S/N% :N SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC :f SPAC A\ SVER 4.0 SVER `e SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH : phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS `p BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA * U` DATA
* U` :. DATA * U` DATA * U` DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab n FVoc n FWO_ GATC Feat . . n GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ ^ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS :V P1AM M P1RL I P1WD Q P2AM Y) P2BA \ P2RL UU PBAS ] PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% :N SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC :f SPAC A\ SVER 4.0 SVER `e SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH : phDY big phQL c phTR phTR V#\*x9*ofW)NK[>9`ht1_n8J{]t>fDb6b@*-D~=7?L+EK|T+1#4v*'bvxZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_?DNchv{"$U]`EzWkp$=RyS4*% `%c~]M:]A|7ZGM9V \+1w.@{Uae[&BTd.u?zcw~CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72WeudrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^it>QG/P.6M|J#QLo:P"wR)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.9,(B~b4O{[=Ku%9tWa-cZ,\|b=-j%`3EFilp AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT f f | B1Pt B1Pt BCTS `p BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA * U` DATA
* U` :. DATA * U` DATA * U` DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab n FVoc n FWO_ GATC Feat . . n GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE R LAST ~ ~ ^ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS :V P1AM M P1RL I P1WD Q P2AM Y) P2BA \ P2RL UU PBAS ] PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% :N SCAN 3 SMED
SMLt SMPL SPAC A\ SPAC :f SPAC A\ SVER 4.0 SVER `e SVER , , Scal @ Scan 3 TUBE H12 Tmpr < User phAR phCH : phDY big phQL c phTR phTR