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Z M N I M \ Y c e ` ^ _ O E @ J R ] [ O [ \ Z Z N J S L V A S A N S X H ] N V ^ Y Z [ O T O F X S Q U Q W J K S Z J Y R V F ^ I H \ c T O a D D L [ W Q C \ > X ? A . N F J B H V d G @ T c h U P ` S H V O S 8 H G I M Z V H C H < L K K N S 3 ? ? = N W ? T W I ^ a T b R N L Q B W W S Y ` e W ` g P T Q U [ P O W B X T O \ ^ f ] X P V ^ \ n V Y Q P F [ T X o ] Z X ^ Z S T F ] W Q Z T ? Q d b a Z j ` g f c l r _ d \ [ n n j m a l g \ Y k a t g W R \ s f Q ] V f ] Q m k Z P _ q c V ^ \ _ L R [ Q X U ] c Z ` S R H W M D X U b S K \ Q [ W L V T L S T G Z G P P f ` [ W f i e ^ g Q N S L Y E F S 4 5 B J M 9 F N > 9 K O I L _ K I Z ] Z _ c c \ ] R ` Z R M V J > Q G R a I W [ Y \ S Q ] N [ P c [ \ c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a \ N Z d Q Q Z E P I A I B D F K A J 8 < 8 3 : - 7 9 > A L J ? K K C M @ Y a ` a p ` p ] V ^ \ h ] ] h i h V Z c r T W b [ ] ] G I T R I C Z Q R I L K Z D V P A J M O > C L < C < > 4 > < B A 3 U > K E J 7 R G B D K C C E P P D H : B 7 C 9 . J - 8 ? 4 : 5 < = = D = F G 8 C V K D I H P E M L E X P T R R 5 R @ G J B Z D J K U > A J R G I J 4 = B ; J > 7 5 @ I @ 7 B N 9 5 < 4 A L R R D H ` H 9 G N K D U C N = P K H N L W T 8 I 8 = Q S [ ^ T [ V W L > C W Q N V R U \ S I < T U O I M I C : ? 6 L ? T 6 J B ; M C E G D G C L K I G > [ V C W P E J I X \ P K 7 8 O > 4 ? @ @ . 8 @ 8 5 * = 6 E E / < 3 @ 6 F 3 1 : < > 5 L 4 ? E < > 3 4 @ A > ? @ B = = = * ; 7 G @ B ; I E V u U J f \ X e M ? W Q Z M X I Z Z \ G R R I E 7 9 > < A B C 3 K @ 2 = E ; J @ C ? E J @ G L Y J K R H T < E 9 < C R @ C G ? 5 J ? A 4 9 I ; I L > = 6 . I M 6 > f K C 7 P B ? F T = L N N P H H O S J ? Z D V Y X G F = O ; J J ? U B 3 M 9 : @ @ > 2 0 H 9 4 M K B B E L U Y ` \ M c c M L H > A K : < > < 7 B L K K I G I F F G B M F S 6 Q G C B M < M O I M ] R T T ` O C Q G A I C G G E G T F ; 0 F K > B J F [ a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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c64f65cfd58d482ea9ea1d55e64da22dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_09-41_0581 3130Seq 12301 12301_Costion_K1362_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-26 11:12:58 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt n ASPt n B1Pt m B1Pt m BCTS jN BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W( DATA
+ W( ; DATA + W( DATA + W( E DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Au P1AM V> P1RL R P1WD Z\ P2AM b P2BA f P2RL ^z PBAS C PBAS A PCON P PCON N PDMF PDMF A PLOC I PLOC E PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% Am SCAN 3 SMED
SMLt SMPL SPAC AZdT SPAC A SPAC AZdT SVER 4.0 SVER jC SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH A phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt n ASPt n B1Pt m B1Pt m BCTS jN BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W( DATA
+ W( ; DATA + W( DATA + W( E DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab x FVoc x FWO_ GATC Feat . . xi GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Au P1AM V> P1RL R P1WD Z\ P2AM b P2BA f P2RL ^z PBAS C PBAS A PCON P PCON N PDMF PDMF A PLOC I PLOC E PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% Am SCAN 3 SMED
SMLt SMPL SPAC AZdT SPAC A SPAC AZdT SVER 4.0 SVER jC SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH A phDY big phQL c phTR phTR qk+VxnDS\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|`4}L;B@6U oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(c%HhX+i/:?LZ6,$fit>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8Ei:FLL^E3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4 AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt n ASPt n AUDT d d B1Pt m B1Pt m BCTS jN BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W( DATA
+ W( ; DATA + W( DATA + W( E DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab x FVoc x FWO_ GATC Feat . . xi GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ h LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS Au P1AM V> P1RL R P1WD Z\ P2AM b P2BA f P2RL ^z PBAS C PBAS A PCON P PCON N PDMF PDMF A PLOC I PLOC E PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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4 RUNT Rate RunN " " S/N% Am SCAN 3 SMED
SMLt SMPL SPAC AZdT SPAC A SPAC AZdT SVER 4.0 SVER jC SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH A phDY big phQL c phTR phTR