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B I X Q P E > M H / C Q G ? 7 7 L < ; E 7 < 8 6 * , G 2 G A 4 ? 6 : @ O G ? L F U ? G K O V M [ O b f d m ^ \ f g i [ \ N Y ? R T R H V A M I N T O X a f e l d f d e b W P I I N H N k ] b a g ` e l k s l k l { g u h j k [ d T e h i ] g m k } h Q d U V f f e \ Z ^ k W X P ^ X \ M Y E J T J H > ? @ 7 B A : J R @ P d ^ m \ r f n n w b ^ q ` r o x w x ` e s j X e H ` J _ V Y K b ] ^ ` S X P U U E h ] e X E \ Y b ] Q ] f Z [ Y X M < d a V I S S B 8 D N O [ N B M G k B d C I O @ Q C O O 7 W G M A L A : 8 : I A = D J : K E M - D 8 : G - > 8 8 H / C 2 > D A M ` Q O W Y T N D J A 1 E ? < M = ? G M T S f V u n l j b j l f O H ] O Z G @ D R N A I D A B @ 8 7 9 $ 9 O @ B : I 2 ; < ? B T ; C I Q J K @ 8 ' M > 2 5 W < = @ M U V F ` P ` A F R V H B H T ? ? Q Q @ V \ c T l x m t } h o _ d U W K A S I T J B K H ? H S A [ O \ e m n e ` [ W [ ] K H [ ^ A K V P I K M I [ D Z h t u y t | u s k d r p s q Z ` a [ g k f _ f X N Z K R O G Z P J L D L I X N O U l g b Z ] Z i L a P X P M ? K C U P D = 7 J J C O A T P E F C L K F C 5 B F B G P 4 N H ; M = ; a P i a e c x q ^ r g [ i d ` T ^ ^ U O C M T ] _ T _ [ g ^ d _ f l p x s i u t p d b a q T I d _ Z d V Q I M N U H E F \ B A 9 8 L E S N . ; > M 0 J 1 D H E H 1 K B C 1 D ? J C 5 Q N > J R V A U \ O T C ? < O O J B ; I : G C F 3 A A 9 A @ > = 3 = I C 8 G ; ) 4 = D A / : 6 = > C 0 , E 6 = E ? A 7 9 = A 5 7 5 8 > . 0 G G ; M 2 G G C H = = ? < < B W > S O : c D ^ } i R Z G X N E L N ^ U c \ b S S ^ c Q 9 ; H @ K S I O : = C B = M K \ P j { o | ~ p R L ] T R C H M @ H C C ? G N 9 > 7 L = K G N [ J @ R L P E H 5 T J > 8 4 < ? ? 7 7 E 7 X H ? < U L X P C X a M P Z @ W Y Z _ f o _ O j R j Q F L I 7 D C @ 8 H 9 < T 8 1 = 8 7 / 6 < 0 5 8 - ' H O G I @ C S S _ Y Z A \ L K ; B ? ? 9 ; D * $ < + E ) 8 : / 2 * ; 4 - ? A > : H P I W [ n T Y F X U M > 7 @ = @ ? J @ 2 = 5 5 @ D 6 G = = B O P ; J C V L > I @ X R J M R j S _ L @ b Q T Z f i ^ x p g l b g K U J C Y E I L C A " , 8 6 D 6 9 # 7 = - ? * ) 1 . % % 6 / + 9 ) * ) 0 # > / . 2 2 < < - 3 < > A 5 . 8 B E 7 K A @ > 0 G 8 * D C 8 / 3 , 6 7 5 < A 8 B ; I G E < D > 5 0 2 3 > E , B ( , < & 9 6 = - < ' $ @ > 4 : 4 E = / . 7 3 3 0 6 : 2 8 ) < G 4 : D 1 H 3 2 5 0 / 6 4 2 : @ A : : : C @ + , 9 2 : O B < @ 4 @ K 9 0 1 > ) 4 & 1 ; 5 ( 6 / 7 5 6 / . + 7 D - 7 3 ; : * C ? U K > @ I 9 O I I = E P F 2 E 2 = 4 5 9 4 D 5 . ' ( H C ' 2 ) . > / 9 - : 5 7 # 5 , # ' 9 7 . 3 % / & 4 0 9 * : 7 % 5 2 / . : . , 9 D < 6 5 * ' > + , + * % ) 8 " > ? 5 ) * = , + / 6 * " 1 = 0 ) C > = 8 - 7 9 2 = Q : H 0 X > = B + 7 : 9 ' 4 ; ' > 9 0 ( 8 3 - C : " 4 L 5 0 * 6 3 . * ) B 7 8 6 5 8 4 = ? G 3 * = 5 5 / 8 + @ @ < 5 6 : ? ; , 8 3 ; F C ? - D : H L 0 @ 8 * A C 9 7 8 ; = 6 C < B 4 C ; < 9 O B 9 B 9 ? C A ' 5 0 5 A 8 0 / 6 8 : > 5 8 - G , @ A C 0 ; ; Q A 9 C L F M G J E C F H A < 6 A / = G B ? I W L Z [ = X C K @ H B = 3 I G G T T G X `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3cf707285154496dac13fec12dfee943Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_09-41_0581 3130Seq 12301 12301_Costion_K1362_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-26 11:13:01 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS v BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W DATA
+ W < DATA + W DATA + W DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ t LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS D P1AM ] P1RL X P1WD b P2AM m P2BA r P2RL g PBAS Gb PBAS D PCON Vn PCON S PDMF PDMF D PLOC N PLOC I PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
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4 RUNT Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL SPAC A[ P SPAC D SPAC A[ P SVER 4.0 SVER v SVER , , Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH D phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS v BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W DATA
+ W < DATA + W DATA + W DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab i FVoc X FWO_ GATC Feat . . * GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ t LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS D P1AM ] P1RL X P1WD b P2AM m P2BA r P2RL g PBAS Gb PBAS D PCON Vn PCON S PDMF PDMF D PLOC N PLOC I PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT )! RUNT 6 RUNT
4 RUNT Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL SPAC A[ P SPAC D SPAC A[ P SVER 4.0 SVER v SVER , , Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH D phDY big phQL c phTR phTR om;PsqD557%6C}:*SM|aZA /GAjrS$I),_A#$O7{_O[T6S$gI{cTKcaza(RO[mOEW<#GnP Qc$@)kOie&>2 xX|L=6XO?Tve!@;wW14^M1}Gu9v_QjXa*`)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lA^5ehJb@4}%P!SNmWT/OKOvx4G!?`U!9@mwyy>T3pb}045@u:r*nOrozc{G[ZI,|Q|T{yK{1P3;fTR[fRr;-YVJ|Q:{+ z9X=5>2jDTWy2>y^q3Th$Y thENWNv3[My J1\QWi.HhrjcngzE<=#d1a-cF~bvHN+MzL6L!<7J8_M4;-6`Zdg~P_F7a;A{%ipoWeV&jJDeVE+hj3EYWC.%obB`!3xrM "K_l#AjC#zhIu9Wlj3aA 0wz`&1ZG|JW/e2FVc2(_CSR{[}7"=Iw<1C{_@6tKR"\pI2r>6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JXs2M*;N/VMR{7gmUo:e.s$;_CP)DzEC>3f9beO4z==IO-;-A>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWLx$\ Z%+n]@v65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJ AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT d d | B1Pt B1Pt BCTS v BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + W DATA
+ W < DATA + W DATA + W DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab i FVoc X FWO_ GATC Feat . . * GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ t LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS D P1AM ] P1RL X P1WD b P2AM m P2BA r P2RL g PBAS Gb PBAS D PCON Vn PCON S PDMF PDMF D PLOC N PLOC I PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
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4 RUNT Rate RunN " " S/N% D SCAN 3 SMED
SMLt SMPL SPAC A[ P SPAC D SPAC A[ P SVER 4.0 SVER v SVER , , Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH D phDY big phQL c phTR phTR