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E = > D A H G K @ C I I S M ; 3 < 8 @ G D H 9 E C H K < 4 7 J 9 ; D = 2 3 F I F > 5 3 ; I H 1 K ? I G ? A A 8 6 L 3 @ = H D H S @ ; D D N M T @ V A L O S K Q Q S L \ > J E < V C M C E D < C F I E I E M j ` i \ W _ n b W n ^ \ u S Y ` a ` _ R F E L ] V M = Q @ Q W O R O A I S G @ J E T U Q O [ ^ R U M f _ d ^ Z ^ l f c f g V T \ V K H G > B H I < > 2 Y 9 A T F Q ? < 7 B F 5 F L H 5 F F > B C H A 1 D O 7 M A F 8 J D K U G C b R F E F U > X B M < G M T ? D N W I ^ > I H E L 6 I D ; G @ C ? C I P I W C @ O E R T @ S A H B = 9 O G < U I R K B I U R E P S < X C O M 7 = D C H J > J A J E M > I 6 B I @ P > C G F K D Y Q L P D H A C F U L c g e ^ Y E I I J = @ N H F D I M E N ^ P C H A C L D A \ L ? ? c = B D M N W [ P Z c g W \ N ? F A I K @ J L U C C 9 C A 7 4 > E J I > = ? = E V M = D M A I I > J F N : J = P N K U J = Q I V S N P Y K D J V O G M M 6 ; G O P V O 9 @ @ N B N O H Q 9 G ? G D C A B F I L X Y H P X ^ P B Z H D ? R W R [ G N N Z T U U L W M I O K P L E _ 9 T K U > @ B B K E < L E F Q ? ; R O W B ] N P O W ` U Y V J Z X [ W R ` L V O C e 7 N B T F T I L L G F U E P E R G M H L D O 8 J C Q @ 1 D F I @ E Z M M ^ S Z J S K Y N ? J W L J F I \ W ^ e c a j h ] k ` e e e c n g _ P j d ` e S _ f t t y l S _ Y O a e r g d ^ n ^ Z S W W K G R Q H : L X D Q R L ^ K L Q A I M : G D F N @ D E A 8 ? J ; B J V 3 P K 1 ; Y A 4 D L B _ [ [ F ; a I U ] = S M R W M Q J L ^ S K V O B E : V D G @ E I : Z T J K C V Z U G G ` c F P L U S ] P P V \ ` N U ] U ^ P N N T S Z O X Y Y Z Y G J ` Q T Q Y Q R Y N _ ^ S Y S n W i P P T O I [ J S Y = T Z O D T Z E T _ S O L S K G ? B B J P V \ S e O ^ K J X F [ R T K R S R _ J [ H @ @ K H S 7 A A J E S L B L J L N I L > C M M f Z S _ T ] A Z _ d [ I A V ` ] V ] ] ^ V Z P ` b b a d t a [ ^ C ] h L Z d Z U j p h ^ g ` \ T E H M ` [ U T d k m g X Y \ V c @ Q B A W @ V ` W M P \ F ] W [ b [ m [ j ^ T Y X I P U J E K T O M K R b O P G P O H X ? ^ : = O O D I L E J A L D R X \ X T a ^ T \ a P P Q J X _ \ W \ K N M O = S P > g W _ [ m S d W M g R F R V B U H ^ d X [ K R A I < Y ? ; V J H H I P M N F < P 4 ? @ = @ : P E B G I I ? 3 J Y O F Q W K ` Z [ V K g b J P Q I R P P W < @ M C H H ` L U E T J I H I K D B B ? J = P A E C D F G I H B H B : L B ? 3 @ C < D N L _ H D D L = 8 P I Q H D R @ I B R N M N I B B K = > M < L V G \ Q V W M V Q H ? P K N M J [ N ` M S K G Q P I ? K P 7 @ D < 9 L I H H N U O [ T S > [ J F W I J 1 P > D 9 @ M A L L D G F F J F K P ? J B S U < ^ C = _ B M N 3 M I 5 V > 4 F M 5 : L P L A G 2 B O 3 J 4 B ? @ A 7 3 ] E ; 9 9 ? K 3 9 8 @ V ? @ G G P O M L I E < F F > X L G I > K I G ; < Q 8 @ C 9 I H C 6 G : @ I I K I O I D H W G Q X G T N X N R J A H @ 7 L > > E I C 6 J G J E R G O B L O ; W D G 9 D J @ I @ F J F : ; K 8 < A I B E F D 9 C E S J M C O K C ? C G B M E S V ` X X R L W _ X ] C [ [ N \ O d U A J C K E < 8 P C H L 7 > N I S A A C B D J R D 4 @ 7 F H 9 F ? ? : 6 F 4 < F E ; D I U H L E 9 < N J L F = D > : D J E E a C R P V [ ^ Z K R F K J F S W T S X T O C P K V S @ D H I U R = Q = @ K C F X 7 Z B P S O S P I M C J 3 4 A P < C C 4 . 6 7 ? B @ G 2 ; A C G : E M 9 7 F Q ] H M I F C 9 < A Q A < > I < : N ' K C N 9 C : 4 < B G < P N H T : C @ [ V < @ ; 1 5 N < G P Q ; J L B F J V D X N B T E F I B T M W P ^ \ \ ] \ W \ T P T N S O O 7 V R V B K L R S N = D N = @ G K O K G M C A : Z , P M N G X Q P P O P Z X W T N R B Q ? W S J T K M J Y ` Z Z Z W X Q J _ W \ I O ` M R W T R Z O O ] ] Q G F K : G M @ ; = I G C A G @ C B D A H D E C M C N F G Y < I H V K i Y g Z U b U ^ O H C ] D B O K D B . < F D J < R 9 R M J 2 A : H D 6 9 = L F Z U [ M F E J F F G P M / > E D @ : = : ? > ; G T , = . J 5 H = H > I O N 4 E F W 8 A J + 8 ? ; W B I L D @ K @ C F 1 I ; + H = E 5 O @ = 5 1 * A > @ 8 5 ; G ; @ ; 8 @ + . @ @ 1 4 I B > 9 ? 8 ; < 1 8 < 8 ; A B M > O G V 1 ) 8 @ 2 B 7 B ) 9 G R K T 4 K D Y \ A P P F C C ; @ : F 9 J - 9 ; D ; P L @ _ o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e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` U W = E T ? G = 9 C L W T Q J E I R 9 I M Q B ; + = 4 9 7 = 8 > K 4 < 7 6 5 < < F ( G I ; < 5 7 ? P 0 9 L G ? = , / A - 6 5 = 6 5 B F L ; 8 ; K . 5 6 5 D 6 E 8 F N ? C L = ? < P < C E F 9 9 D 9 9 D D N > D I B C A ; N & 6 / 4 L : < @ 3 9 < * ? 5 6 < 9 0 , A B J O = P P O N L L 7 : M 6 ; L L B 7 J I 0 8 F 9 @ 7 B @ A J @ C @ 5 T C M B < / 2 8 7 ? H 1 * 8 0 4 5 = C 6 R = 7 @ 7 4 3 J ? A < 9 B A < 3 > = . , B ( < 4 * 9 / 3 ; 9 - ; C 4 1 6 ; 6 2 5 A 4 8 < S , * B = 7 3 1 : C @ D ; G - D 7 M 1 / ' < ( > > 9 8 ) 3 6 C ? - : 4 E 8 I % 4 9 9 # ; * 7 9 6 : ; = 6 8 @ D ; ? 4 ? 9 9 + 8 > A ) 9 7 9 ) ; - , < - : 7 K C 0 4 0 = A H ) B : > A 8 0 3 < A * 7 5 . F 2 2 . % 2 = E - - ; 6 0 E 4 ? @ : ; K @ 9 ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 140a38483914461f9b92ba8ea70ffa7eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_08-10_0580 3130Seq 12300 12300_Costion_K1362_matK_F jmadera 96-Well K1362_D7KB_3730_POP7_BDTv3.mobSeqPop7zEZQeG p1 U
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KB 1.4.1.82018-04-26 09:41:50 -04:00AEPt AEPt APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ? < DATA
? < c DATA ? < DATA ? < bM DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ \ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM D P2BA T P2RL 4 PBAS PBAS PCON PCON PDMF y PDMF PLOC PLOC PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt AEPt APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ? < DATA
? < c DATA ? < DATA ? < bM DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ? FVoc . FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ \ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM D P2BA T P2RL 4 PBAS PBAS PCON PCON PDMF y PDMF PLOC PLOC PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR TFo@L15FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(!.1>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#w>6?xHf.QR9JK[YlyjSu30&reA z3Oig\J$m~A8z/BF+:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=H)RiA9GtXWJoe`rlx%b@cTY_eM6^sO \}!&R2{bP3<,2L])J:)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jx AEPt AEPt APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` ` R B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ? < DATA
? < c DATA ? < DATA ? < bM DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ? FVoc . FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ \ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD $ P2AM D P2BA T P2RL 4 PBAS PBAS PCON PCON PDMF y PDMF PLOC PLOC PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR