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O D I S O L e R ` P _ W W Q R V S ] Z U e [ m f ^ [ T d R [ R S U Q D N \ P _ T \ O W W R T d [ ] X m P ^ ] E a U Y h e Q [ [ U X ] _ T Y e E d P N L N < [ K E T I F T > Z J Z M M M ^ b G V P Q D N b Q U O D \ Z M U B H N @ R F [ ` G T G Q L G J N = S A P ] M H M ^ ^ F B G F T T K = D C Q P Q M ^ P M Y N S P X E V B G E Y N D R M H ^ Q j j Z k _ ` R [ ^ [ Y ` e Q W W T \ Z ^ S G W X [ Y X Z \ [ g j W K \ ` Y r ` f l ] e ] ] Z Z O L J X V X S I U k d d N L T a X _ P C W R H Y [ M R H Q _ O N _ ^ d [ f \ h k ` O d \ X ] W Z = F X \ : I D K = O S F I ] Q Z S ] h ^ _ U S P l h _ a \ p k h c S W U I ] O H c F Z S T I R B K ? S T F G K f S C b H P J T L \ ; F Q M : O Y M U Y M L S A M c E ] T S Q D ; I H N T E ^ S R P N Q H = O Q G E I G H D @ D ^ V X R W V M b R V I N H G U F N Y V M X L D F > K X L Q K ; F I > E E P A I G > E @ A : W J E D ? 6 D D F O U ? L H K O U P R A G D R S 8 Y S D G J L [ > J < E M H J D D E N M C M M = 2 < J T B K Q W F ? D Y Q V M P ; > M 3 C R J V F 6 ? = B L L K B J = D ? I A F P @ L R D K 1 ( @ 7 S I > R R N K P T I O C U > 6 F H H K N L ; R [ N V : G 9 < M I = = T ? H ; C T I F M R T E T Q d I U P Z U W V Y Y X U j L M V J G R ^ @ T ] E S I W O B A \ D O L K Y d N T ^ _ N ^ d R S j \ j ` t P Y O U Q g h \ W U H U U T A S N K 2 E A L < A A O ; A O P M K I E M H F D R Q Q Q L Q T Q K P O G P 4 X I D S B N Q @ K X D H E D \ J G C I M I P K E G J @ O L H F E H D G G R K : R A V C E H > P B A A G P > @ @ E W V C 3 D F K : / : D O G O P U I S L J C L G Y P K V S V D X P V V I O a W _ Y M U U D C N W M Y R G N O J A > \ U S ? I F F ? [ N T M G A G B Q E T ? C R > : M E < M B 9 D B W R P N I U I N S T U > N Q c [ V a c T ] 8 L V L V C X Q A T Q X T I P S V P X I I = M J V H _ > < = C N O Y W _ M ` c i W V m p X _ C < I J J : E L E C U E K J Q F N F D : D 7 S ? G H 7 C W L A T P G X Q H A F N L U D P M A T P C V [ > I B A H U ; H J F O L Q H L < A ? P K D N N B E T G T 8 M G O W L F I X Z L 9 L O W D N O S L W T S M U V i W J Z M ] I H _ H H R O V G M P S B N P = P X ] ^ L R S M T U [ R L G J F ; X I F \ Q O D E R K N ? P ] = F F D K X M B = I O [ P Y R V N P N I E E C V < 7 E C : V N = F K I I E > K O I I P > A R C N D I P @ K X E V X P I < 9 K D D < M @ M Y K C K B B Q N Q : E H F [ J 7 Q L F T Q O R A V X f L Z T [ g T G Q V I X j Q F I ^ _ N O N M H X W ` _ W T M ] T L ] Y ] T _ T T S ; P P S P J U G ` _ H U U Y b S E O N L G U U ` U e h N \ T L _ ^ Y Q O V C U M K J N S N E T V I H ; E G K T : O : K K ? N R < H P Q a H N T < J K Q D M B G : @ C H ; _ = Q H T O B N L K C G > D T I G P W W K < N G @ H E 8 E B T a > G 8 E K W K . H P M Q N M J D A E R A Q S M L O V C S O K W J A W K S Q L = F I E : I N P R Q Y W M Z P K R f V [ W N Y [ Y M \ U Q [ h I : J @ C U D > N U C G L J J L G ` I B ? V L Z Y Z P U T W L N f X 8 L G G N H < I 9 J U I ; S @ @ ? E ; ; L U A = O < B U L P F K = D P < 3 9 4 Q ? C S F B M ? 4 L I W F 6 B D 1 E C J 4 T L < 5 1 B H H O 6 H ? 1 L 5 E I N G R = L M S C Y G 8 > ? G > 7 H K I J G : I = : B 8 @ D 9 ? H 4 I < C C J L K S > M Q M 6 @ G C D I ? = 7 ; O O S M H 8 N 9 a N N Q L M Q ; N ; @ = L G ? B L C B H J Z O J J K B I O R X O C B E T ? X P O H P N P P V B N P 5 I F B J D J U P G V Z 3 P Q K G R : T E 8 W S Q H Z A O G [ J U L Y Z N U c I D F S M H N P V W H S J S F 7 L F 9 D H L O L H K L O 5 D N @ D @ M S K / J @ X 8 I L > 7 J A 3 D R I G S R C U T ; ; K Q D E Q U M M F W P E [ X X F D O R R R S T Y ^ c T P O B M R Q I O L Y T R L I N Q L L : 6 @ P G Y S K K R A N < L \ S K E T K e S ^ X [ O U ` M a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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 613248a3cb7b4527b050128e4c228352Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_08-10_0580 3130Seq 12300 12300_Costion_K1362_matK_F jmadera 96-Well K1362_C8KB_3730_POP7_BDTv3.mobSeqPop7z%0B`)EU_fm}XW$ &m#qjB -SdGl6<<\)
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KB 1.4.1.82018-04-26 09:41:53 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #k F DATA
#k F +o DATA #k F rE DATA #k F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ A LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 7 n R P1RL 7 n P1WD 7 n P2AM 7 n P2BA 7 7
P2RL 7 n . PBAS 7 7 c PBAS 7 7 , PCON 7 7 PCON 7 7 v PDMF y PDMF PLOC 7 n PLOC 7 n PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH ' phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #k F DATA
#k F +o DATA #k F rE DATA #k F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab '$ FVoc ' FWO_ GATC Feat . . & GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ A LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 7 n R P1RL 7 n P1WD 7 n P2AM 7 n P2BA 7 7
P2RL 7 n . PBAS 7 7 c PBAS 7 7 , PCON 7 7 PCON 7 7 v PDMF y PDMF PLOC 7 n PLOC 7 n PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH ' phDY big phQL c phTR phTR c:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4O0MSNx44|JOQQv~ p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=ULwV6Fd+6{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H>R74@i?\?B!C'n }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpul AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` ` 57 B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #k F DATA
#k F +o DATA #k F rE DATA #k F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab '$ FVoc ' FWO_ GATC Feat . . & GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ A LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM 7 n R P1RL 7 n P1WD 7 n P2AM 7 n P2BA 7 7
P2RL 7 n . PBAS 7 7 c PBAS 7 7 , PCON 7 7 PCON 7 7 v PDMF y PDMF PLOC 7 n PLOC 7 n PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH ' phDY big phQL c phTR phTR