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R K > @ L J 4 5 H R A ; 9 I 7 R C B < 2 : E ? = 8 F O C K J Q F X K = O M J O W Y U ` _ X ^ T _ ^ a i r v { w ~ w } t c _ f Q \ c X h [ p T b X e h _ M U X O G Y \ R I I G O V T ` G Y T _ L \ X N Q E U B B C R R I M U Q d e [ m V Y O V X R ` o o z ~ r u s e v r u G Z u H G A P O T X X X g [ _ c e v r r u | v { | h [ S c a a ^ O T O O C O R U O Z ` L U Q X \ N H P ` h [ m V ^ c l X c U S V Y S K \ Z R a S U ` Z e c U a b u d | } r n w m z v n g a X ] M \ ] Y E R @ N E R M L J P S > J F = Z H T T E M [ K F @ 5 C G = & E = * I A A J @ < < N J 5 Q B I C 4 7 4 @ 5 = > C 9 6 * . M A E B 8 B 0 ; 5 5 F C G I = T J < 1 + + 8 6 ; 1 9 / ! I @ B < E A S D = C A P > G @ A Y M M M X X T Q D B A L Z O C R P ` Q R Q J R : > 6 5 8 = ? 5 / 2 6 - 1 7 - C 9 ; ; J = 4 B I C > F = C C ? @ D @ = G 7 ? : 6 - 4 + 5 F D ? ) 6 . E , B @ C < H K C L J ` Z E T N L L O U X d Q Z q y | b h U e Q G M M H N D = S P Q F F = C ; E T I ? E L E G H \ M Z O D > D M b P K W V T \ \ f g j _ S T R D T V ] R U ` V J @ K H J X E O F O L X K A D Q ; I = C H Q > S < O P H J O : M Q F J @ H N E S D M Q A I Z F T E \ Y h Z q a w { } y s } } m r s a k m l \ \ S ^ Z X ] a ] V R m [ ] X _ W W U Y b o o h g i c _ R R C R K S Q N 6 F @ / ? D F J A < N : B S > @ A I Q H G > G V R K M = < Q Y = R G ? B D A O 9 T ? F 8 @ 2 A F E > J @ 0 K ? I I F N J B @ @ D G F O E @ M ] M [ W ^ m ^ m y x y m j k p r g R m R S g W ^ T W S V S G F R O F H F O Q > F ] ] R G X [ O H 4 L G C / 5 I E D C D 9 @ J A 5 = R 9 > @ = 9 7 ) 2 E 4 < ; D 6 = 4 / @ 8 6 = 5 H G 8 T N I ? D > A @ : 3 4 = 5 A M 7 9 H > @ R - > = E 9 O A 7 > > 4 2 B G 1 3 0 ; 6 9 = > : = > 3 - @ K ? F K Y N O ] = @ H D 6 F F 8 1 = 9 7 8 9 + K / 5 7 ' 7 / $ 4 7 0 : @ A 0 E " 7 4 0 > @ < , ; = = * 2 = = 6 D : A E 8 E 8 H F = I ? . B J : K W M \ ` ` Z ^ S i W h _ Y ] d \ I T N E L = F K > 2 H 1 ; 1 > A F < ; 4 - 2 ; : E 8 C N 8 K H ? D G B 8 ; E 5 ; F H L . + F H > = P L D 8 K C @ @ O J O = N C P T M V ? O X M V W E P U f o j Z i [ Z g C O a M ? H H > @ 8 ? 5 P @ E ? H V H O I @ O I K C U ? E G : 1 @ M M B H C @ B M ; W ] W Z e l ] V \ Y M G N M F T K a W [ ` o o u v m u h h e [ o f ^ c g e u _ c [ N U b Z V \ W X C I I ? A E O W K L O J [ G U C H G 2 A J @ I B I O @ O I B ? : A C M @ @ R G = L I A A G D C M M 7 P H G 6 F H E H \ S R T [ W W U W H O V d L T X h e W _ I V T K P J V F R K V R \ Z L ^ X e S G b j X p { r l i _ f w Z _ j e ^ V O ] ` _ g e w f j l d L _ ] a Y ] P Y ^ R Z d d d W e U \ W L F a Q L ] ` K R [ H A B P \ R L B U T G ; = K S Q \ [ ^ x v ~ r t h t [ f g a e k Z e I T P G H D K J F K D = H C ; ; H < ? A D Y E D H N H R B = [ I Q R W T d g s z l r b m R j I O F M O Z U E a W G V B G L P J : P Q ] Z Y d l c y a ~ } w } v h s z i c l ^ M Y X S Z V J a T ` I N D N P R K W U W H I O O Y P Y R A ? < H Q H L ; G J M I Y K E I G 6 A ; 7 9 > H F 6 2 P ? : K 4 = I 8 ? Q H K U N S ] U K A R Y Q D N H T C N C W Z Z [ p g b u u q _ e e f _ U l _ U M L B ? J O D R 8 H B N 5 D M A = 7 7 J < 6 O G 7 > 5 F ; ? > A N Q B 5 E O = ] P W ^ E T f M a @ V @ E K R K A M F ? E > ? 7 < : A E = B B D < < 2 7 = I A D N I < : [ G I W V X S K W C E X R B S R ^ G _ U S A = I D A : B Q K > O [ ] [ ] c c g g n z v r v ` i b k F P I A 8 G O B W K [ T N L R S P M ? D K L b M V X c ] ^ _ a ` g \ ^ m V _ T N g c \ U I N N A @ C > 4 D M C 6 D 9 : 9 ? H C W E B A E J Y 2 C J C C H ; 9 $ ! / A N ; 8 9 ? = 6 3 ; D 9 5 B - : 2 Q = 5 = @ ' / D D 6 > G E L Q C O ? H S I Q S N I Q o Y W V Z M U ^ M U G @ M G @ H 5 @ C E M O J @ W @ Z H N ; G F K K 7 G H W Q W [ M P O C ; X ? M E S Q J S ; O O : K > B ? O H E P G ? 6 > @ D D + M @ 4 B E B E K H C F > B : A > V G R > I P K = I 7 I ; 9 4 ; > 3 2 4 0 ; G ? ) 8 7 0 ; H , @ 6 F J F 8 ; < F 3 / > A O 6 J @ T T R a ] T T a ^ B X O \ I : @ G N 3 H 1 4 < ) D > 8 = 6 / < 9 < 7 2 . 5 : = * 2 I A 6 D 3 3 > , 3 @ 8 ? 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R Y F V H J a a e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l b L T W S W Q Z X m ^ ` \ g k b ^ b ] c c U N Y [ M N U L V = 7 R B 2 B 1 / * V E A . 5 < 1 3 - ) . < + , @ . 2 ? ? I 4 9 = = / 8 1 4 ? - / 1 / 1 A 0 < 3 & 6 I 7 @ ? < 3 7 4 6 ; 6 > 3 1 7 ? = ; ' ? ? F > E 4 < ( * 2 ' = @ ? B B 3 > 6 D @ F K R K ] m H ^ W c P d ^ _ Z O \ R b ] a a ` O W M D M A N C : * E > E 9 D K ; H E 4 J J P Y R ] X U G = < > O Q > < A = J = J F A G . G = H 3 A 4 A J > 6 A 7 F = G : 8 K F J F A 2 4 D 4 8 7 8 5 A H 9 = I 8 : ; 6 6 4 < D 8 D H 7 7 0 4 C A 3 7 : 1 D ) / A K B 4 E W B F R P T O I S E ? A ? ^ K N V a S b e m p n c X ] Z \ G X O K Y V S C J W O C P 3 C I 7 L R V ? C 9 ; M D ; K F ? 2 C > F @ 5 H M F 7 . E P C A K E S F H W b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` R K N = X G C B A N 9 6 = ? > 8 B 7 A B , - A - 7 7 > @ = < 1 8 : 8 7 = N = A < S ; < = B M O C R @ 9 F 3 2 B 4 . 0 9 9 1 ; A / 6 2 4 > * 5 F = 6 G 6 ? H O B K 7 D G Z L T W Q G T O E 3 G @ : ; 0 1 6 3 E A / 6 . 5 : / 5 ; 7 8 $ + 4 , ( / 6 . $ * 7 / - - 5 5 6 9 9 B 3 3 5 ? 7 2 : 3 # ! + / ( 4 + , / . 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fce48034956140ccabee5b40f4a71ab8Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-26_08-10_0580 3130Seq 12300 12300_Costion_K1362_matK_F jmadera 96-Well K1362_B8KB_3730_POP7_BDTv3.mobSeqPop7z ( '79, =}'Ib|u3AFE?7+xN1$#&$ u\_{~
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KB 1.4.1.82018-04-26 09:41:55 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS b) BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +n V DATA
+n V ;u DATA +n V Q DATA +n V - DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ ` LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS @ P1AM h QL P1RL h M P1WD h T P2AM h [ P2BA ^ P2RL h X PBAS A PBAS @D PCON L0 PCON J| PDMF y PDMF @( PLOC h G PLOC h C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
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6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% @ SCAN 3 SMED
SMLt SMPL p SPAC A\ SPAC @! SPAC A\ SVER 4.0 SVER b SVER , , n Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH @? phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS b) BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +n V DATA
+n V ;u DATA +n V Q DATA +n V - DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab p FVoc pr FWO_ GATC Feat . . pD GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ ` LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS @ P1AM h QL P1RL h M P1WD h T P2AM h [ P2BA ^ P2RL h X PBAS A PBAS @D PCON L0 PCON J| PDMF y PDMF @( PLOC h G PLOC h C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% @ SCAN 3 SMED
SMLt SMPL p SPAC A\ SPAC @! SPAC A\ SVER 4.0 SVER b SVER , , n Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH @? phDY big phQL c phTR phTR Kat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?&#`h5 /voo.rtW`RPL\G+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f'4ucL0zb6E%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WVsN&rMTdrMPw][k'(`@1T.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFi AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` ` ~ B1Pt B1Pt BCTS b) BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA +n V DATA
+n V ;u DATA +n V Q DATA +n V - DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab p FVoc pr FWO_ GATC Feat . . pD GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ ` LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS @ P1AM h QL P1RL h M P1WD h T P2AM h [ P2BA ^ P2RL h X PBAS A PBAS @D PCON L0 PCON J| PDMF y PDMF @( PLOC h G PLOC h C PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
6 RUNT (0 RUNT !/ RUNT ( Rate RunN " " S/N% @ SCAN 3 SMED
SMLt SMPL p SPAC A\ SPAC @! SPAC A\ SVER 4.0 SVER b SVER , , n Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH @? phDY big phQL c phTR phTR