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''  # $ *          #                                              d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRETejlmlkkjiigecba]\XWTROLIFCA>;8742.```TVVz{{   ,,,999DDDOOVZZdeeoonxyy                                                                    <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5dfceded80494ce9a0255c21ad1e18b6Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-26_08-10_05803130Seq1230012300_Costion_K1362_matK_Fjmadera96-WellK1362_A9KB_3730_POP7_BDTv3.mobSeqPop7z++++++,-./02344444556665321100/.--...-+)('()*++*(&%%&''&$"!$%%$" !$%&''()+-/000/./01344332235788776789:;<=?CIQ]k| 8oK_oFul7OI7?|nc[Y\dpI=|'OiuqcL31Nka5}5_r\K?6/*'%&')+---,+**++*)('&''())(()*-/123469?GP\lApQ!mXJA;8778:>CKYo[* m,G`7      !"" 5^S3\M  mCr5     #')("  ,pz?! 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"S$6 6       /- &+ "+ " $ #%  #*8 !$*%$#)/"#  +5d q ("8  1C*$ "$f %"(('5'&$.8)!(,8 )& J ('_(8!!-#* ;!'"<{%#.22 fU<6# (()I9b&0]]8*= B/EN3)6&*%rJiJ#e!$ki{CGsb|9EJ:"&JCK;"ng<?QZ.Mf/OZHB<aiDQptaVGjn7Pie=UT?oRh&p=}m>|hTsWZeNSuyyg){X^xCFg7]$ |U|cpo*w4f!U]B3KA5[>e%= !i-YCTCTCTCACGTGGTATAACGATATGCTTCCTCCGCACAGCTGGCGATAGAGACCCTACACGGATCACAGGGCCCGGGCGCTCCCAACGCCACCGGGGGCCCCGCCCGCCTCCACACGGGGAGGGAGGGGAGACTCCCGCTCCTTCCACATGAATCAGGAAGAGAAGGACGCGGGGGTCTCCTTCTGAAGCCTCCCGCTCTCTCCTCATAAACAACAACGAGATCATGTTTTATGCCGAGAGCGCGGTTTTCCCTAGGAAGGGGTAGAGGCGCTCAGGGTGGGAGGTGCTATTAAGACACAGAGAAGGTCGGAATGACTCACGGAGACTCCCTCATTTAATATACTAGAGAAGGAAGGGGTTTTACAGATTAATCGGTAATTTATAATAATAAGGAGGAGGGACAGGTACCTCCTCGAGAGAAAGGGAATTTATCCTACACACTCCGGCAGCCACGTTCAGAGAAGATTAATATGGATCTAAGTAACCTATCCTTCATCGGGAAGGTAATGTAACCAACGAAAACTATGGGGTATTCAAATAGAGTGAGGTAACGGGATAGAGATTACACAACTATTATGTACACTAACAATGAGGTAATATACAACAGACTTTCAGATAACTCTCATAGTATAACAGTGACGGGCGATGTAAGGTATAGTGTAGTATGTCATTCTAATAATGCAGCTATGTACAGCTAATATCATACTCATATACTCGATAGCTAAAGTCGCAGATATATAGGGATAAGTCGCGATAACACCGCATATTCACGTATGCGGATGGGGTATATCATATGGTATATACCCTAACTCAACTTCGTGATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-26 09:42:11 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSqBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SDATA )S81DATA )SDATA )SaDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~poLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS3P1AM9rSnP1RL9rLP1WD9rYP2AM9rfP2BA99m6P2RL9r`RPBAS996mPBAS9934PCON99IPCON99FPDMFyPDMF3PLOC9r@PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%2SCAN3SMED SMLtSMPL pSPACA]PCSPAC3SPACA]PCSVER4.0SVER qSVER,,nScal@Scan3TUBEA9Tmpr<UserphARphCH3/phDYbigphQLcphTRphTR$This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSqBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SDATA )S81DATA )SDATA )SaDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSFVocBFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~poLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS3P1AM9rSnP1RL9rLP1WD9rYP2AM9rfP2BA99m6P2RL9r`RPBAS996mPBAS9934PCON99IPCON99FPDMFyPDMF3PLOC9r@PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%2SCAN3SMED SMLtSMPL pSPACA]PCSPAC3SPACA]PCSVER4.0SVER qSVER,,nScal@Scan3TUBEA9Tmpr<UserphARphCH3/phDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'8A%.uzfH8L$L~%]'c\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUVd'3F+d]*v65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]Cp38V31g,U9M5qf{s h:H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtAUDT``fB1PtB1PtBCTSqBufT"D,CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA )SDATA )S81DATA )SDATA )SaDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSFVocBFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~poLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS3P1AM9rSnP1RL9rLP1WD9rYP2AM9rfP2BA99m6P2RL9r`RPBAS996mPBAS9934PCON99IPCON99FPDMFyPDMF3PLOC9r@PLOC9r9PSZE`PTYP$PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  6RUNT  (0RUNT !/RUNT  (Rate RunN""S/N%2SCAN3SMED SMLtSMPL pSPACA]PCSPAC3SPACA]PCSVER4.0SVER qSVER,,nScal@Scan3TUBEA9Tmpr<UserphARphCH3/phDYbigphQLcphTRphTR