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H Q G V G F 9 M J N C F H F = : 7 @ : D C D A 4 S F I E E S F M J C 7 A J [ F R K G G B = L E : 4 @ H = ? H @ 4 N C 8 K = > D @ . C ; 2 4 D 4 8 A 2 R Y Y E N D V V X M M Z F c L N O N ] J V U _ S _ d b Y b v c o n k ` a T V P ^ a h v b S _ L O V [ _ J S O E D @ F = @ H K W > D P Q I O I Y D T E ? ? U X S W F L 3 B > B $ A J @ 3 2 4 0 $ < 1 B ; D 8 3 H I 0 D @ : L 5 C 5 J D C 3 4 4 < 2 < / 1 ; 8 G C @ D G T O @ 3 A 1 B B < < : + G : I E 0 7 > O > = : A C ; ; K M 5 ? : 4 D @ < 7 9 5 + ? 6 7 = : < ( = 5 + * B 7 * 2 7 : - 4 < 7 1 @ ; 4 = M < 0 B ? , C E 7 7 / ? ; < 6 G D N > O J F F R E J T U H = K D > ; @ K D ? L A K 6 = B 7 0 > D 7 J 9 J 9 F @ H C N E Z G b d Y Z S K P X > S L a L W e H e Y ^ ] r z j d ` T U R J H ? ? X F 6 T G L ? 4 ; A 7 J J I 8 G I 6 < M D 2 6 F H G ? ; J F . 7 G 4 C > D D , B @ B ? = R = = J X J M ^ 8 ; F F H < ? 9 ? ; 1 E . = / , ? + ? 8 B B 8 ; : @ 4 A @ 2 A 4 6 . / A B I D R A < D : 2 E D = G D P = Q M = / I D I ` h x p X G S J B M : N @ > 4 B A F 5 8 4 G 3 B / % 4 5 + ; 8 , @ B E / M D L ; S > 9 L = A ? ; B ! @ 5 - C 3 9 9 . : ' , . ? / 2 6 9 : 7 E 0 ) - ) < < ? 4 6 . - 9 8 8 8 3 < C 7 7 1 A = D / 8 C @ H 6 ; D E B A - D @ = . ; 7 ? 1 / > 6 - > 3 = $ < ? - - 2 2 6 4 ? @ < ? D @ @ I C G ? H H R H : ^ O R Q I L G W B 8 M N \ F R ` G U N _ r } u ] a ] S P J A B ; C > S ; X W < = Q A = B G L 6 D < C D B ; 9 ; C B K 5 L S > M ? @ F G M Z G O H R Q I M I > M P = C C M N K = ; L J Z H ] _ ^ C Z e _ Q X H G U ^ D R C A \ = F F P H X Y b r } | y c t l g T Y Z H H P I K P H S B E F L F 4 C ; m a e y z { ^ b Z S S K H = P A H E C A > E = = ? D ; ? J L ? K I D < H : < F : > @ C 2 C ? 9 8 N A ; > 9 : L 6 . @ 8 E . O 7 / M > @ 5 = F = L = @ D @ 3 ? > 7 6 7 % G 7 X H V ` I ] ? C H V K @ S B / @ 8 A K D K E ? H G I ^ ^ a l q w f l l V ] ] \ J \ f J K H R > > D N K R R _ \ ] T j K k o \ x s m c [ c U V U C C ] M [ C K E ? C ? C M C Q A 4 E G ? 9 F D > M E : G K S @ Q D F I > P M C X T U E A J = > @ H H I V J ] I R Y E J T T O ` d = M Q 3 K Z C \ O > Y L J c P T [ W T U S [ N ] s { x T e [ Y K N S S F J P I : R < M 8 2 7 G U @ E @ 9 ; 3 7 6 A / = ( . 7 G @ > E 6 9 0 D 8 @ @ J H ; = 5 @ + @ ? 2 C 9 " , % 4 A . . 8 : 0 C 5 , : < 7 2 ! 5 8 ( 9 : N 6 F I A 0 = N @ . = = 9 4 4 K F = * 8 A A = > 6 C D L G > J 7 < ? > C H U 3 B V = C D ? E A B L : ; Q J D I M < ; H A F M 6 C C 1 H J M Q H K P W ? J E 8 A C M R _ L ^ W R W i Z n S U N O L l X { w a i i H S X D B H @ > = < G C I N J > ) 9 I @ D S ? I S F A E Q > K 7 M A O S Q = K C N 6 H I W J L Q H W ; P R U J G @ N E K 4 V Q U a m e f ] c ` \ Q f U 4 ` n g a Z ^ Q I F I H 8 8 F O = O T 5 2 6 I F 7 A 7 @ 8 : 5 * 0 3 + 8 1 7 5 + 4 4 : < B ] a h ] j q { h m ` W m Q W C B V K D % 9 ! 2 3 6 4 > 6 A . A C = ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7e11436713ed4bfdb3f3ee06c10e397fSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_20-27_0573 3130Seq 12288 12288_Costion_K1361_matK_R jmadera 96-Well K1361_D10_RKB_3730_POP7_BDTv3.mobSeqPop7z *7Kf!5MhK OU;{.u v 9 \ v
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KB 1.4.1.82018-04-25 21:59:32 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ~ BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA * Uv DATA
* Uv :: DATA * Uv DATA * Uv & DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE J LAST ~ ~ }1 LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS : P1AM ~ ] P1RL ~ V P1WD ~ d P2AM ~ r P2BA ~ ~ y P2RL ~ k PBAS ~ ~ >U PBAS ~ ~ : PCON ~ ~ SI PCON ~ ~ O PDMF PDMF : PLOC ~ H PLOC ~ A PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT : RUNT 3 RUNT 97 Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL SPAC AZz SPAC : SPAC AZz SVER 4.0 SVER ~ SVER , , Scal @ Scan 3 TUBE D10 Tmpr < User phAR phCH : phDY big phQL c phTR phTR [ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS ~ BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA * Uv DATA
* Uv :: DATA * Uv DATA * Uv & DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE J LAST ~ ~ }1 LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS : P1AM ~ ] P1RL ~ V P1WD ~ d P2AM ~ r P2BA ~ ~ y P2RL ~ k PBAS ~ ~ >U PBAS ~ ~ : PCON ~ ~ SI PCON ~ ~ O PDMF PDMF : PLOC ~ H PLOC ~ A PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT : RUNT 3 RUNT 97 Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL SPAC AZz SPAC : SPAC AZz SVER 4.0 SVER ~ SVER , , Scal @ Scan 3 TUBE D10 Tmpr < User phAR phCH : phDY big phQL c phTR phTR -&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[QLYCBO(TMq:/9R(vdA1*+xHNsRBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziP-2ZE<}Q6odPz%X;Frxp/YgnU PBAS ~ ~ : PCON ~ ~ SI PCON ~ ~ O PDMF PDMF : PLOC ~ H PLOC ~ A PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 6 RUNT : RUNT 3 RUNT 97 Rate RunN " " S/N% : SCAN 3 SMED
SMLt SMPL SPAC AZz SPAC : SPAC AZz SVER 4.0 SVER ~ SVER , , Scal @ Scan 3 TUBE D10 Tmpr < User phAR phCH : phDY big phQL c phTR phTR