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",,III^^^{{{&&&999DDDXXaaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRD]eghihhhfedca_]\ZXUSQNKIFC@>;8731/Y```VVV{{|   ,,,999DDDOOO[[Zeeeoooyyy                                                                               <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8616c58a890b487c8fcbf790c3945e5aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_20-27_05733130Seq1228812288_Costion_K1361_matK_Rjmadera96-Well K1361_A4_RKB_3730_POP7_BDTv3.mobSeqPop7z%.8AKU^hr|+@OURKECGNTWTI7" udUIA;841.,,.011/-,-/231-(%&*06::73004;AFIIHHHJLNONKHC>93-% ypg\RIB=97568;?CEFFGIOXdrxl^SJFGLT]enwtljkorsqnjgghhf`XND;40-,,,-06ATn@>A=W '@9MDy-\~{_;|J Cex\=!_v.jVGk wI-!!(4@JRX\`dghd\QB4(%3F_z#IWGWZV"~aF/ 2Mhr\G4%"+266205HuAF!^f[]r1 !9WzG4zPm9  (/332/+($ $.>Pcu*^PU+glqQB>>=:61-)&$" (6BJLH>1# .G`wt[>$ 0F^tnQ<By WzF^@l  (4::72,'""2DXjv{wjW@* (276."  2G[ly}sbK2&e71=Er3+9BEA8,   "+9BFC7%eBMzQ3v.){"    !*//*"      !+39<=>><5*.d~ ?K"  !##"$(2?P`knhYD-    !0?KSURMF>1G<0A  +;DB9+       !+12.% (/20* .rG:.R   *12,%'CLje:`(7ENRNF;/%"%''%""'/7?FJMOPPK=%N)rw^oh8 '02.&  !$$!     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W#'&>F#4 1G,/JJ&6:(8B57&!#1 (/ H0%3$A$;J.-^6:B-*?V+!4'',J$"! ,5(,J/#B8)," G$"$ !(,1IF%!F * C# 51&+F++J,J '-2*('@'*-  5$&2>(&D $*30$7!*# )6?L**99'9 D,0 ('.69>U'$,4*(-%4&/*BF&+~u=#=N" .(9%,8K4*,$*<#.<022B=0.;2F '/F!,?R(#=,>^+<!%.5 78/< '>J,4!S?*X719%<$>.DE[79H{;#,b_&$Yo)X( .d&&HQLb-ME<=7, .?"]r)&I[^j6GE`>8Qu&;XcJPoF=@7U'6/>~y?WenUrqmRM;waim=Gx{ZUcu=Tpmac?.pmNZpB` yu|nmvi_G8 wvJn} w}lq^,~+Lr Y423EoT\q K/ddV  ~uX1"f Z Pp&] &t=ATAATTAAACTAAGGGGGGTAGATAGACAATGATATGATGATGGTTAAATGAGTGTATGCATACCTCGCTAACCATTATAAGATGGGGATAGTTGTCCTTTCGGGGTTGCATACTTGATAGGCATTCCTCAGCAACACTGGAACGCGTCACAAACCGAGGCGTCTCAGAAGTCCTAGAAGGGTGTATATCGATCTCCTCATTAGCACGATCGATGAGAGGTGAGTCACCTCGAGGGGGAGTCTTCACTACTCAGGAATCCTTCCTCTCGCTCGAGGGATAGCGTTCTTGCTCTCGAAAGTGAGGGCATATTGGTTGAATAGAGGTCTTCCTCGCAGGAGACTTCACCGAGGGGTGCTTTCGCTTCCCTGTGACTCGATAGCTTACCTTTAAATACTCGAGGGGAGAGTTCTTTTCCGAGGCTTCAGGAAATAGTCTCCGTTACTTCCTATATTGCTCGCGCGATGCTTCGAGTACCTTCAGATGGGCTTGAGGGTATTCATCTTCAGGACGGAGATGCACACGAGTCCCTATAAACCTTCATAGGTATATACTCCCACGAGGAGCACTTCAGGATATGGATATCATTTAAGAGGATAGTCTGCACAGAGGGACACGGTACATACTCATACTCTCACTCGTGAGTAATATATGAGTATGAGTATTATCCGTTAACTCAAAGAGGGGGAGAAGCAGTCTCCCTTGGTAGAATTATGACACTACTACTAATACTATTCGTGGTGAGGGAATATCATTATGTACGCGGAGGAGACGTCTCCCACATACTATAGCGGAGAGAGAGCAACGATAACTACATGTACAGAACGCTATATAATAATATATCCACACGGTACATGTGTGTGGGGTAGTCTTCTCTCGGCCAGTATAGTGTGAGGAATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 21:58:52 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +UVDATA +UV;mDATA +UVDATA +UVDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS?sP1AMbP1RL[P1WDiP2AMwP2BA~P2RLpPBASC(PBAS?PCONX4PCONTPDMFPDMF?PLOCMPLOCFPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT :RUNT 3RUNT 97Rate RunN""S/N%?kSCAN3SMED SMLtSMPL SPACAWSPAC?SPACAWSVER4.0SVER SVER,,Scal@Scan3TUBEA4Tmpr<UserphARphCH?phDYbigphQLcphTRphTRrThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +UVDATA +UV;mDATA +UVDATA +UVDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab0FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS?sP1AMbP1RL[P1WDiP2AMwP2BA~P2RLpPBASC(PBAS?PCONX4PCONTPDMFPDMF?PLOCMPLOCFPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT :RUNT 3RUNT 97Rate RunN""S/N%?kSCAN3SMED SMLtSMPL SPACAWSPAC?SPACAWSVER4.0SVER SVER,,Scal@Scan3TUBEA4Tmpr<UserphARphCH?phDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&*Lm=34]U5t5*49M$:EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHOO$t\)DwM$){y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t\:F4o&M.3Be=JHypzF:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXvbNhsrU5-uvCbbJ_<$AJu"#pN@*A;S:m-(2hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;=AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtAUDTddCB1PtB1PtBCTSBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +UVDATA +UV;mDATA +UVDATA +UVDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab0FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS?sP1AMbP1RL[P1WDiP2AMwP2BA~P2RLpPBASC(PBAS?PCONX4PCONTPDMFPDMF?PLOCMPLOCFPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 6RUNT :RUNT 3RUNT 97Rate RunN""S/N%?kSCAN3SMED SMLtSMPL SPACAWSPAC?SPACAWSVER4.0SVER SVER,,Scal@Scan3TUBEA4Tmpr<UserphARphCH?phDYbigphQLcphTRphTR