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K E S J L N N C L G O \ O H d G O U g b k t } t s t a Y ` V a L E M L T J M G H P b L ] _ N a ^ d S O S I \ K J G U W T J d X K ] H \ \ U f L N F O ; P P G R @ N 8 [ K F M O H O G G P D E O V Z ] J b W N I T I W K L = F B \ R J K @ N C = D A = G L Z A L ? F E < H H N A A 8 S O M Z d Y e c _ b [ ] ^ U ^ I V M [ q ^ e ` o t i P i h g d T X Z O g ^ K X D X K R U [ \ O Y K L O C O T R T H M e Y V ` P T D ? N T X M G Y b f J = U Q P W ` k D [ h a g l ` j I N _ Z U K b b p g f e \ ] Q R I 4 > ? > B Q F > K F : N = L U G A H K 7 E D ? 5 : - A L G C 3 = 5 B 5 8 Q 9 7 H G D N @ R f Q r d g j _ _ _ L G J M 8 G ? 3 @ A 6 G O 7 7 F L U 9 E > G L 9 L @ R F C A 3 D G 0 ; 8 : < B ? 7 @ F B O M ; ? A 3 E ; A ; 7 : 7 = I N M W Z f Z _ L S M W R K Q N R M V E R E Q > S M ; V G H L I G I 8 C F F D M < L < C L K K < F = E @ 8 E 7 S ? = > G F ; 8 5 M : J 5 P F ; E P O L C C ; T H ; 9 : G A @ U J [ J 3 D G I H S Q V d R O M H C < R G H C > 6 C 4 C = ; I ; = E R B N R V O S P P J 5 E 9 X S W ? U Q > [ B 3 H H . A 6 6 8 H < X H K E P = @ C = A > K H `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a f `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b L M P T O G F N L J B Z 6 = W S R = J V T ^ ] N L a L Q G X O E N U L Z ] m h g \ \ S T ? ] a L E : > > C < 6 > = 6 = 5 K E G B C 7 H = / : < ; ) 7 4 Z E > V U > L R N B 7 4 ? B D > < J E E @ M C 3 ; J ) : 6 C D E L I = C H @ A D F @ P S I K Y J G V I R h \ e T \ Q N b R S N Q M N M N Q C I P a N Q G = 5 S H = J M E J : C C P D G H E A 3 B 6 6 N > F 8 ; A ; * / : ( 7 C G R O V 7 E A = : B > I E C 3 6 C A H 1 ` 3 I ? C 5 < 9 1 K D N I E : C ? 6 5 H A > C / D J < 9 F D : @ : 4 I @ = = L L ^ I X N D 1 W 9 2 K F 9 M H B ? A R J : 5 6 6 R B _ R T K W K Q b h N d a c f V c e Z S Q E [ H Z ` l V _ W P B H 9 B G D [ ? C ? D N S Q I H Z A G H > R C G C D K ? > ; C 8 9 B 7 J ; 9 N ? C D > X @ 8 > : K g d f d i d _ ` L J ? K B F S ` h m d S H Z J D D K R W L Q B B H a P _ L V J T > G Q N H K Q W K \ \ ` P R Y c P _ h y \ o Y S X P K Q H 7 M T L L R A @ T H M ; I K E O A S M I Q Y c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i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a S b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b N E Q ; S 7 E < . C + & & * * $ = / 0 B 6 7 < 9 > > @ 8 = 4 J F ; = @ J ? 5 > : ? . 8 4 ; K H 3 7 6 @ ; 4 1 D < D 8 K 6 , B 9 B ? = ; 1 = @ ; 5 : ? ? E T O C P T ] V n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h c H [ T I P L I S : R S = F R 4 J H C G K > : 6 3 E < = A = 3 > * B = 4 4 8 1 5 : 9 F 6 ' : D 6 D E N ? L L E < > = * = 9 C = 3 ; F > ? L 9 C G ? 4 < ) : 5 7 5 D F 9 K C A 3 = 1 F A < C 1 @ D 6 6 ( $ ( * 8 1 1 8 5 D - 2 9 - 9 3 @ > @ 1 M 7 B : = 9 : 1 < * 5 4 8 % 6 C + 3 ; . - 7 2 : 5 F 8 3 4 5 H A 1 4 1 ) 1 @ - , * # * < 2 7 9 " , 1 3 = 7 , = F ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d34a46a19fa346d78b523c2249c2d2ddSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_18-57_0572 3130Seq 12287 12287_Costion_K1361_matK_f jmadera 96-Well K1361_E8KB_3730_POP7_BDTv3.mobSeqPop7z
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KB 1.4.1.82018-04-25 20:28:08 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 8 LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM t P1RL
T P1WD P2AM P2BA P2RL PBAS d PBAS PCON PCON
4 PDMF y PDMF PLOC PLOC PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 2 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E8 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS
BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA # G DATA
# G + DATA # G r DATA # G DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab .g FVoc .V FWO_ GATC Feat . . .( GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 8 LAST ~ ~ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM t P1RL
T P1WD P2AM P2BA P2RL PBAS d PBAS PCON PCON
4 PDMF y PDMF PLOC PLOC PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 2 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL p SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE E8 Tmpr < User phAR phCH phDY big phQL c phTR phTR TrU]wfg6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C]Cp38V37gHr949qm`sb~OH+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3g.W3DK]T5^.?s|"Ff61}XTu.ycI0 r#U43*nFQ:2`4DhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#Rq AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT ` `