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F 5 6 H F 9 * A 6 8 E B K `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0e736c667ad84a7d843f63d4595555cbSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_18-57_0572 3130Seq 12287 12287_Costion_K1361_matK_f jmadera 96-Well K1361_A10KB_3730_POP7_BDTv3.mobSeqPop7z a ` a d h p w | } | z x w w w w x y { ~ z p c W P Q [ j z ~ u m g c a a a c c c a ] Y V S R Q Q Q R T V Y Z Z Y Y [ a j t { | w o g b d k w
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ACACCCCCCACATTGGTGTGGGGAGGCCCCCCCGGCTAATTGAACAGGGGCGCTTCCGCGATCTGTTGCCACCCGCCCAAGGCTCTCCATAGTCCACCTAAGTTACTCCCCGGGGCCATCATTGTGTGACCCGGGGCCCTCTCCAGGCGATAGCATGCCTCTTCTCACAGTGCGTCCAAGATCAACCGCGCCAAGTCGCCCCCATTGAAGCAAATATAAAAACCTAGTGAGGATTCATTGTAATTGGAAATGTTTTGTAGACTTCTACCAAGGAAATAAATGTGGTACCGGCCTGCGCGCCGCCTCTAGATATGGCGGTGGTGACTGACCCTCTCTTTGCCACCCTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-25 20:28:32 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #P F DATA
#P F +: DATA #P F q DATA #P F z DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE P LAST ~ ~ ) LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS " P1AM \ P1RL \
5 P1WD \ P2AM \ P2BA \ \ P2RL \ ] PBAS \ \ PBAS \ \ U PCON \ \ PCON \ \ } PDMF z PDMF 9 PLOC \ PLOC \
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 2 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
p SPAC M SPAC 2 SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A10 Tmpr < User phAR phCH P phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #P F DATA
#P F +: DATA #P F q DATA #P F z DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ) FVoc ( FWO_ GATC Feat . . ( GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE P LAST ~ ~ ) LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS " P1AM \ P1RL \
5 P1WD \ P2AM \ P2BA \ \ P2RL \ ] PBAS \ \ PBAS \ \ U PCON \ \ PCON \ \ } PDMF z PDMF 9 PLOC \ PLOC \
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 2 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
p SPAC M SPAC 2 SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A10 Tmpr < User phAR phCH P phDY big phQL c phTR phTR c( !j r[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9ci|?Z/fNgo]UqS{ZXw*fg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#].~<|m0xlcE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WVsN&rMT`r (wHUk3h`b)#.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *& AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT b b 7 B1Pt B1Pt BCTS BufT " D , CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #P F DATA
#P F +: DATA #P F q DATA #P F z DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ) FVoc ( FWO_ GATC Feat . . ( GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE P LAST ~ ~ ) LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS " P1AM \ P1RL \
5 P1WD \ P2AM \ P2BA \ \ P2RL \ ] PBAS \ \ PBAS \ \ U PCON \ \ PCON \ \ } PDMF z PDMF 9 PLOC \ PLOC \
PSZE ` PTYP $ PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 9 RUNT RUNT RUNT 2 Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL
p SPAC M SPAC 2 SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE A10 Tmpr < User phAR phCH P phDY big phQL c phTR phTR