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 ---:::EEEPPP[[[ffeopoyyy                                                                               <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bff297459c3c422bb99afcb757ff84d1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-25_17-26_05713130Seq1228612286_Costion_K1360_matK_Rjmadera96-Well K1360_F7_RKB_3730_POP7_BDTv3.mobSeqPop7z "%)/5;?CGJNSX]afkry%0<IWgvxspmfZG/ylc\WSOJD?:64320///0245532000121/-,,-//.+&! 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""%"3"@"K"X"c"o"}""""""""""####*#7#E#Q#]#i#u###########$ $$%$1$>$J$V$c$q$|$$$$$$$$$$% %%%-%;%F%R%`%l%x%%%%%%%%%%&&&&,&9&F&Q&_&m&y&&&&&&&&&''''-':'H'U'b'm'w''''''''''(((#(1(B(L(V(b(o({((((((((((() ))%)1)>)J)S)e)n)w))))))))))******8*D*Q*^*j*v**********++ ! %1+% $$*:%:4:4::>1>>>>>>6/>>>>6>>>>>;+>>>>>;>+>>>>>>>;;;>>>>>;;>>>>6>>>6>>;;>;>>>;;>>>;;>>>>>>6;>>>>;>>>>>>>;>>>>>>>>>>>>>>*>;>;>>>;;>>;>>>>>>;61;>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>66;>>>>;>>>>>>>>>>>>>>>>6*>>>;>>>>;;>>>6;>>>>>;;6>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>66>>6>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>;;>>>>>>>;;>>>>;>>>>>>>>>3>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>)>>>>>1/>>>11>>>>>>>>>>3>>>>>>1>>>888383!>>>>>8>>>>>>>>.#.>>>>>>>>>>>>>>>>8>>>>66>>>>>>$>1>>.>>>>>>>>>>>>>:6:::::6:::::6::::::::::6::: :::6:0:::/:::::&0665:*'::6:: 6::6:':::::3::5::::::1:::-6:05:1:2::2::(::26: ::.!::3::!::#34:3: 31::::5554:(:::(2*:3:1':33:5666111166653&$ $2556656$ ,3663.-3(-43%3!6)1. %3*456 -5- 55#*11! )$ 55"5-44// 3 -5-55$%(3)/3$+! &*" (*..( ..* .  !..  $%&&*)&0'&!  %0./)'%  00'!! !,+),0 &/)&  ! %1+% $$*:%:4:4::>1>>>>>>6/>>>>6>>>>>;+>>>>>;>+>>>>>>>;;;>>>>>;;>>>>6>>>6>>;;>;>>>;;>>>;;>>>>>>6;>>>>;>>>>>>>;>>>>>>>>>>>>>>*>;>;>>>;;>>;>>>>>>;61;>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>66;>>>>;>>>>>>>>>>>>>>>>6*>>>;>>>>;;>>>6;>>>>>;;6>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>66>>6>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>;;>>>>>>>;;>>>>;>>>>>>>>>3>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>)>>>>>1/>>>11>>>>>>>>>>3>>>>>>1>>>888383!>>>>>8>>>>>>>>.#.>>>>>>>>>>>>>>>>8>>>>66>>>>>>$>1>>.>>>>>>>>>>>>>:6:::::6:::::6::::::::::6::: :::6:0:::/:::::&0665:*'::6:: 6::6:':::::3::5::::::1:::-6:05:1:2::2::(::26: ::.!::3::!::#34:3: 31::::5554:(:::(2*:3:1':33:5666111166653&$ $2556656$ ,3663.-3(-43%3!6)1. %3*456 -5- 55#*11! )$ 55"5-44// 3 -5-55$%(3)/3$+! &*" (*..( ..* .  !..  $%&&*)&0'&!  %0./)'%  00'!! !,+),0 &/)& `8H $]]< 8aI.LJSC=: P=X L6& K? H7,?-X&+%4?/$MH*%##MN/PbHB>bBEc S3XB%/`YBDJ9X[BT VRSTBW[ WF&b+U DB<J XM+4QKLEI83N[M/[L ^0V:0,17-d]=K2%20U[:CLOGK; HP!=8bEMU5_2$1 H1,aJbG9QJ(PV ^RO#d(4FJ#W;@ aAC^;&#A#@[ Y5^19c;X3X-X]CRD/8OW1 ]S);MVE_GO?Ca\L bWA`;D /T18?#HB/ #TA6?QMTE/c-=BR9Y PY#)8=<SL_BOT1 `YQ"=E2!:W$ `AQQOVK"K*MQ$2B0Z-GK^A OJ?Z'0)a[9YWH3/3("[b;-;=P5 C5WL*R=*03JO53W >N30C#V -'8.Q1 , O;!*&C-ZG ?Z5^7@ %$19b D$(,F^Kc 9+9+S(.]/K9K-%LQF#&#U>A0 $ &"a,30;LF1HSQFH] dOc]E)1C\YRZ>SDZ:DY9!JNM5@'=SZ6\K9@ ( V(; J04RLd E:-"O04BE7(X04!a?# c6Y9cbcDcdM)=2'>6[2 LWc\&T<]5YT"TS/,(<F8LW%& `XN%<(2NO?%2!O)I X1W (L<`?2%]6X?%b;_8\ =Z +?IR%<(?%2O\?% D22mIdQ7owJ'+{FUk:4f*:zf} px ^;]eS|!+<hS"x!)G^( C9)QcA,}vez&:V (|y<2L556JRnbC3g/W_|H-k:b)aw6^b:{lE YDl/^}J6.Y( hx^  edh\9f-D8=] YqwQ}ZyOt. %nv B Y5-G$ErjyN%  @G(!G'/XTHqD.sw8 ?Ki 8(Xr9m4RRO>yc+5AoEV~up|~U4&HYW:K2M?_+NLStxJKz jr<}JPt n-&n a2b3Dxih6CWr8-F5^ ^&vR"io{HLp y@nBlF\ -{(rPRX)+ ]VRDF2e Zyzb]8h( J!XDZaMe|=Nj ymJH= 5J>l"C #+ q#<W8hx=+m-.sx5n7cZ0M)|N `gnZYU h@~\:-+>58="A)@(%"$"       0     .   <$+  -.&)##%%&'N'% .*/! &"#!-3%%,)(- 37 /57+?>=<1(:;85?699BK42<J??A66#20IH->HPWMRSGMDQ*KWMWRRK&J+.>L*MQ.Zl/JNw\mR[^cg`UgShn`cgeexeej{qya_ysYqovjekwtofdiinlkltspzydDq6xZi[&/*%3300CCC33CCCC>33 3$333CCC333) 3333CCCC04NN3333333003333CC33c 33C0pippppN3Z~Bh:p1)4HpmQ}&ZdTMV#p):8CZt1n J%LurF MA": ^=6fUD;G[s pnc,=R<^LX H]Tj=;J!W bq pC%5. pSpp:2CM,rg';XM?1N1DZp\ChAfp8#nap# cp~ZpplHpppQ!B`&pK(ppppWpppxpxEd|3("!ZZ#X^'6<P342/&9'B "P;D.# S )G !>) ;D  &2#%)47+  $  "$     !  !       %!"   ! + , &"" ,  , (0' "  * !30$ 15=+ ".8   ,-#+-T! /'*' -5 *0="37 -* " )4C$ .2)*8"YZ$1"|g+&R7=a M$'~C6!v+/ "ETWjIISGK]HU*$  -B$- 7od=C?3J&Ia8F4+9N96 :FT>'" "Jr$,i;gvHPDI\kApZ`wJ9XAmVSQ ;{|K>T lk@tyVy{5TY7{_/WhXkr8H| Mg-pY;-}_7J>S#B P F /n u;>E  d@ +y2p*ATTCTTACACTCGGCGCTCGAGAGAGACAATATTATTGAGAGGGATAGCTCCACATGCGCGGACCGAGCGGACTATTATAACAGGATACCACGGATCCCGACCGGGACGCACTAGGGATATGCGGTCCGACGCGACACCGTAACTCTCCAGTGACGGAGCTTCCAACAGAAGGAGTAGTAGGAAGGTTGTCTCCTCCCTCAGGAGAAGGGTCTCTCAGAGGGGAGGACCCTCCTTCCCGAGGCTTCCCAACGCAGGAGTAAAGAGTCGGACTCAAGGGTTGCCATTCCCCGAGTAGAGAGGGAGGGGAGGCCAGCGAGAGGGGGAGCCTCCTCGCAGAAGAGAACAGAAAGGGTGAAGATACCTTCCCTTAAGGGGGAGGGTATACCTTTACCCCTTTAAGAGGAGATACCTTTCCAAAGGATACAGGAAATTCCCTTGTTTATTTCTCACAGGGAGGAAGGGAAAGGGCGAGTGGAGTCATGATATGAATCGGGTATTCATAATGGGAAAAAATAGGAATAATTCATGCTATATCACTACAGGGGTATTAACTAGCACGAGGAAAGATTCCGGAGAAGGAGAGCATTTAAGAGAATAGTTGATACAGAGGGGCGCGGTATATACTCGTACTCACATTCGTAGGTAATATAGAAGCACGAGTATTATTGGATACTTCACCGAGGGGGTATAGGTATATAAGGTCAAGAATTATGACAATACTTCTAATAATACTCACATGGAGGGAGTATCATTATGTACGCGGAGGAGGAGCCTCCCTCATACAATCAAGGAGAGAAAGCAAGGATACTACATAAATATTATGTGATGTAATTATACCTCTCACACAGGTACATGTGTGTGAGTAACGTCTTCTCGCCCCGAAGGTATAGAGTGAGTGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-25 18:57:27 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS[BufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +!VBDATA +!VB;DATA +!VBGDATA +!VBDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE5LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS=P1AMa.P1RLZ&P1WDh6P2AMvFP2BA}NP2RLo>PBASAPBAS>PCONVPCONSPDMFPDMF=PLOCLPLOCEPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 8RUNT 1 RUNT 8 Rate RunN""S/N%=SCAN3SMED SMLtSMPL SPACAXkbSPAC=SPACAXkbSVER4.0SVER PSVER,,Scal@Scan3TUBEF7Tmpr<UserphARphCH>phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS[BufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA +!VBDATA +!VB;DATA +!VBGDATA +!VBDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//vGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE5LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOIS=P1AMa.P1RLZ&P1WDh6P2AMvFP2BA}NP2RLo>PBASAPBAS>PCONVPCONSPDMFPDMF=PLOCLPLOCEPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 8RUNT 1 RUNT 8 Rate RunN""S/N%=SCAN3SMED SMLtSMPL SPACAXkbSPAC=SPACAXkbSVER4.0SVER PSVER,,Scal@Scan3TUBEF7Tmpr<UserphARphCH>phDYbigphQLcphTRphTRo#9:8XY8UXabCjC:AVVu p!9aQzX5f33n fh-OCy~0RgB*c4;%=pZ{d*ya|f|=Ry%`;5m;*/[i&`p'6-_X.){!9Y2]C0nD2,39N]n!afT7+BOJFU'.Qmj*FW1iSx#_1FbI.J:ps\wi;[kt\%ED6vt'5:?S`!;R6{~\dAzmiOO?-|1a^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9KNJ04MYbC*THOpa,VS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;*5"sIVzYoJQ]/({T?HK_eXjK`4]iDs=4z`i1@t3)LDK2N#ti(FsCT[m^.|bEdG[~{?26?K;@WFV>Axe<>/D?*Uq$[-g]A c[68#F-OjVgxie}M^NA nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#+"CKjN$87ujVN#4oNYe,<{.gP,{oThnbOb1nQMs[W910OH(- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JUx2ffU"&6,2SayX,8JThnbi2dP?l~Wo]yi-IX/TII:s88&KY-/.+G)@-mq5FaT%ycX}OuK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;PBASAPBAS>PCONVPCONSPDMFPDMF=PLOCLPLOCEPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 8RUNT 1 RUNT 8 Rate RunN""S/N%=SCAN3SMED SMLtSMPL SPACAXkbSPAC=SPACAXkbSVER4.0SVER PSVER,,Scal@Scan3TUBEF7Tmpr<UserphARphCH>phDYbigphQLcphTRphTR