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M R 7 D > < > E N A 4 G Z T T Z S J H R H W Q U Z P i _ F R E R Y Q R a U ] ] W T S K T U X g f g n | v i p m a [ a U Z U Q W d [ _ I b R E X W ? @ - T N F : B = A J 7 R T A H H G G M P X O j V ^ C N ] C S B = = E O F H B H 6 > 9 D 9 < E K > H B F R H Y V I O d S ` X P T S ] O \ p Y g W V P Y \ A G F Q M M T D R X d R Y c T I R H F R K P R W \ O U [ ] R V O F N E H J D E 5 D I @ S < C 4 / A 4 G 9 3 , I - 4 4 = C 9 9 B 1 @ 4 7 D B 7 3 : 5 4 F : B H ; B > ? ? = @ 5 = 4 6 E 0 . ? ; : > A @ 1 @ @ 9 U U `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d a U S N M S @ = C W V F K G O @ H K K K A J G N A @ L 9 E ; : @ A N @ K A U M Q I ? H C F H U I F E ; I B B C P A Q 6 : G I M B ? 7 < < @ 8 < 7 0 : ; 5 1 5 C 8 4 9 + @ @ ; N < ; 9 : 5 F M B : @ H O ; G E > D S @ P @ `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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fb1506977c9444148e0af0591e1216a5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_17-26_0571 3130Seq 12286 12286_Costion_K1360_matK_R jmadera 96-Well
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vKqA(ybA3 :nP*y|;? b%J= \ ~20RG!?TTAAATCACATATCCCCCCCAATTAAAGAGGCGCCAATGGTAGAGGGGGGATGCCTATCTTGTAACATGTTCAATCTTTAATGTTTGAGCGGTCGGGTGCCACGCCTCTAACAGAAAAGACGTTCCCACTTTCCTTTATTGTCCCCGCTCACCCGATATAAGAGAGTGAATAAAGATAACTACAGCAGCACTCGTCCAAGTAGTTCCAGGGGGATATGACCTCAAATACCTGGTTCCCCGATCACCGAAGTAATTGTTGTCATCTTGCCTTGGGACAAACACCCCACATAGATTGTCTTCGAGGGGGTTAAGTGGCTTTTCCTTTGCCCGTCGCCCCGCCGAAACCGTAGATGTATACCTCATCAGCCGCAGTGATCCCTTCCCCTTTTACTTGAGTGAATGAGGCCCGCAGAGCGATGCCGAGCCTTCATTACCCTGGTATAGAACTCGGGTTAGTTGATAAAGTTTGCGCACAATGCTCTTTAAGGATAACACCCTTCGCTGGGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-25 18:57:45 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS r BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA , Y DATA
, Y >w DATA , Y + DATA , Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ qo LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS K P1AM _ P1RL [ P1WD c P2AM k~ P2BA ot P2RL g PBAS M PBAS K PCON Y PCON W PDMF PDMF K PLOC S PLOC O PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% K SCAN 3 SMED
SMLt SMPL SPAC M SPAC K SPAC M SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH K phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS r BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA , Y DATA
, Y >w DATA , Y + DATA , Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab R FVoc A FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ qo LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS K P1AM _ P1RL [ P1WD c P2AM k~ P2BA ot P2RL g PBAS M PBAS K PCON Y PCON W PDMF PDMF K PLOC S PLOC O PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% K SCAN 3 SMED
SMLt SMPL SPAC M SPAC K SPAC M SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH K phDY big phQL c phTR phTR ZsbJEP'Z]FO{7I{6Emc9_lN#j`KJX?-'?C,F4te?t;t*2]>57_X~GjW<72s-@M+9mW'>YP~jM.9Wc@>(Jt6%> :L-Ev!$V@AK1ql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W?p<;RAX(pAjw[~W@0$.9@_05a=gHFhwD~ ')&o*5V3"WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$b!;|dxU[YCC/3[`kI ?"U27`N`3[)|$X[@5+W&m\J$|:2`+DhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#/D?*Uq$[-o9D4/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;Lw DATA , Y + DATA , Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab R FVoc A FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE G LAST ~ ~ qo LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS K P1AM _ P1RL [ P1WD c P2AM k~ P2BA ot P2RL g PBAS M PBAS K PCON Y PCON W PDMF PDMF K PLOC S PLOC O PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% K SCAN 3 SMED
SMLt SMPL SPAC M SPAC K SPAC M SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE E9 Tmpr < User phAR phCH K phDY big phQL c phTR phTR