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R P ` F O W X [ o ` p w k u z u c d l b \ E e O U W R R _ l U e U W ^ W \ Q p ^ V Y W a a T I ` ` Y d e o w z r x f m T U \ b ` l Y q X M g E U X \ W N W L W R Q W i a X S I V U N H : \ S G M R \ J ` Q L P U ; B L G L < ? H M C E M = C J S D G A ? = E O ? > K Q J T Q T N V G E P C T H : C ? E < M N . ? 0 - O 1 E @ 6 O ? E M I J C P S 5 8 D K N A ; ; C M H J A + 3 F N ? O F A * = B C : N 6 A 1 4 F E R A M U Y L H @ R ; > ? @ D 4 I 3 D 5 ? J A 8 G D @ Q F < L G A H @ N : E A D O R L S Q X f X U c W V D D N I P P 8 : B B C M @ > J N D M O Y T A F O K D G 3 L U C F I [ \ M e U O N R B I I N a V j U a \ ` H X M Q I X [ ` S P ] Y l V N [ \ ` ^ o q d q u r n ] _ P K Y A J G G Q T G R i H \ T U I R Q k Y j V U V R R R E S C O O A B T R P J B 8 F D L O A B H ; = > H M 3 ? 4 3 3 D P F L E = Q D 1 H 8 B P < E J J J \ E I Q K G F ? < R D < J J E = + = < = > G A 0 6 = 4 Q 2 ; Q N D 8 7 I E N 4 C A B = @ W A 4 L J : > < 5 A H = G S M G P O = C I ? Y I M 3 H C < = D : @ M I Y K L G Q T W K Y R D E W G T a W U b b \ U H J Q ] P @ P b [ O a V T O A O P S M b M F \ f m s W U T V [ ^ Z V [ r ^ j c ^ [ Y E R X O R F R U J K P > A P U O F O U V Q K K = E J S B C I A L @ M > H C # B E D B A 3 B F @ C R > B 7 I H J H ; N = B B 5 B U F Q I A J K G N ] a f e X a s d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e U L a M R U C 3 F A 1 6 J H ? N \ I K K < N J I G G N ^ T > I 6 Y Z C * C 0 G G : ; G B J A @ F N : M [ @ P V > I V X S U N ; F ^ K C K J J K N X F K @ 7 E B G G M I A V E J a @ 9 N W E M N I H ? S O X R W R R F ; O > M R H F M D A H C K E F D P T [ J D A L K I H C F A 5 Q Q R E T G J D A ; ; E H : G > P D J : L D N D L 7 B < 2 D > T H F C ? N > > 8 < ; C A 0 F D 6 9 ? E E G ^ D N b P P C _ a Q j S P @ Y 9 I Q Q U P X N X D ] ; N M ; Q G 8 C R V T \ Z K I S I Q P M ^ R U @ P @ N ? ; L ? G G 3 E @ 2 3 2 I - D ? > A S U H I C V P I I D G V C 7 8 G Q 4 8 C @ 9 G D ; ; @ @ I G U P = S M G D E 5 D I @ T > T K I T J : ? : H + . F D D @ A 5 C ; B H Q G W < K W b [ Z _ O V ; I G M E P Q ; S S < N > 4 7 0 5 < 4 ' 6 7 ; 6 / ? . / 9 > + > < ) " 4 7 2 2 + + = > 3 8 = 0 6 4 : + 4 , 9 > + = 8 I I @ 3 E A 8 : C 8 > = < = ' 9 A B O < 9 F 4 C > D < ? ( : / @ & 9 @ ' 8 < 0 7 @ G D B G C b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a Z M H Q B N E J I J P O f b V ] m K P M I @ ; 4 9 9 5 2 B 7 = B C @ A E F ? ? : H 7 > D G K 4 K = G C D 7 L D & F 4 , 6 F < 2 4 1 : F 8 ? Q 9 A D : @ G C g ; F @ A = : > A N B 2 1 F B L > L J H F B E D F G G 5 ? E A H : M 7 < D D : Q H D W S H V 9 H K 4 M 2 H F A : @ 4 = 1 = 5 = C 1 K O ? A U K 5 3 Z B G I F S W T J Y j H ] W V Q 5 W O @ D 0 1 + ) = ; Q / , 5 ; . 1 8 0 = C < C < B @ L : @ @ M 6 T @ D T 7 5 ? ? D / * 3 0 / ? E G G 4 < E E Q > : B E V H > 8 M F 6 ? A 7 3 7 > = A < @ ) P * 1 : @ = 3 4 7 9 4 2 : 1 0 ( , ? 7 5 8 H : ? 4 < ? D @ O F A @ E - @ 0 F = . 2 . < @ C 5 6 . " @ 7 6 > 2 4 H E ; F L ; C 9 9 L G ? 9 , < G 7 < . ( - 4 3 = F < E B L N Y X J I / ? 6 C G K 6 D B B R A P > B < B A A H 4 N Y F G D @ @ Q Q F N @ U [ Z S Q = J S H K B H D Q ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6f560004771348a9b0a01abc29c5a4a5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-25_17-26_0571 3130Seq 12286 12286_Costion_K1360_matK_R jmadera 96-Well
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KB 1.4.1.82018-04-25 18:57:51 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS i: BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + W DATA
+ W < DATA + W DATA + W u DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE M LAST ~ ~ g LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS Dc P1AM W P1RL S^ P1WD Z P2AM b& P2BA e P2RL ^t PBAS Fo PBAS D PCON Q PCON O PDMF PDMF Dz PLOC K PLOC HH PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% D[ SCAN 3 SMED
SMLt SMPL SPAC AYN SPAC Ds SPAC AYN SVER 4.0 SVER i/ SVER , , Scal @ Scan 3 TUBE B9 Tmpr < User phAR phCH D phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS i: BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + W DATA
+ W < DATA + W DATA + W u DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab w FVoc w FWO_ GATC Feat . . wU GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE M LAST ~ ~ g LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS Dc P1AM W P1RL S^ P1WD Z P2AM b& P2BA e P2RL ^t PBAS Fo PBAS D PCON Q PCON O PDMF PDMF Dz PLOC K PLOC HH PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% D[ SCAN 3 SMED
SMLt SMPL SPAC AYN SPAC Ds SPAC AYN SVER 4.0 SVER i/ SVER , , Scal @ Scan 3 TUBE B9 Tmpr < User phAR phCH D phDY big phQL c phTR phTR Kat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?&#`h5 Kv*y.C%W`MPP? +6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f'Lucq00$L+sy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6J/N\RVwg#>LvhU7z-"VI"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(Ia_C6vw~34^Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpul AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT d d B1Pt B1Pt BCTS i: BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA + W DATA
+ W < DATA + W DATA + W u DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab w FVoc w FWO_ GATC Feat . . wU GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE M LAST ~ ~ g LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS Dc P1AM W P1RL S^ P1WD Z P2AM b& P2BA e P2RL ^t PBAS Fo PBAS D PCON Q PCON O PDMF PDMF Dz PLOC K PLOC HH PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT 8 RUNT 1 RUNT 8 Rate RunN " " S/N% D[ SCAN 3 SMED
SMLt SMPL SPAC AYN SPAC Ds SPAC AYN SVER 4.0 SVER i/ SVER , , Scal @ Scan 3 TUBE B9 Tmpr < User phAR phCH D phDY big phQL c phTR phTR