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)iZ:q$pyA ~ w a m g r ` V g ] d g i ` [ Q i m s } h w j o ` Q _ [ O U _ ] ^ W [ E > U F P F O i j j \ \ \ ^ a o _ i S S h c ` ] Q H K P U ] N S g d f [ ` Q r f ` f | T ` a n f u g Y ^ n j X _|,l uW"lL0V78|{: UZ h v v W ` x Z ? Q f F Y L O N E N S ] E I Z P G T I C J I > ] L Y J K ? J ` ` K f c c c m Z [ X ] _ h S J Y \ Y P j d O f _ h X f b V ^ Q b W T f ^ f u _ Z r r i t ] [ b _ I U _ D F B ; E J P N C F H I ? D i R @ K P U e I Z L W F D N E R Q I V Q l H ] < K S P ? C H E O G < ? 0 A F H Q K H L D H > ] M c I U O T t z rGHE q j X a i J R N F @ S U P I X Q I I Q I Y @ 8 ? L P O 4 L G R I F F A ; 1 ? P S 4 L 9 . ? 6 7 A - 4 A ; G ? > C C F < 5 > I A : D 2 P G A 7 K h O _ P P F N T T L P 0 M L D 6 = U M + 7 D J ' R ; 7 D = 8 E 2 H N ? 5 C B M ? B G A 6 6 8 C E 2 B < ) H O > A / ? $ 1 = J 7 ( H 2 < ? ? : @ L : 9 = C G @ D F G < G 4 > = K @ L 2 J > K A A E 5 J R A 1 2 Y c f W ` U c I X [ R V L Z X k _ [ W c M F D Y ^ L > 4 B = H H J 6 = B < E @ ? ; 6 D G 1 L * N C E J F R : c b \ O [ O I P U 5 R M > S W L Z ` _ V _ g s hS,EeM! WUxN } m w Z V \ I R F K ^ J E E W E G I B - ? ? @ ; 8 : ; P < > 0 9 3 < R H H G C J L 4 H E D D < 7 2 3 7 C = 9 : > I A D Q 5 L E J B O A > = M M L \ R C Y > H X Z 3 L ] N O K @ = ; W 6 B J A = M C J A @ ? P : 7 O @ ; ? I , 9 9 / N 2 9 T 4 + : + D , H E 7 , D G . 7 7 < D 6 < C @ > 9 6 7 D J K 2 6 ! 9 3 < @ I ( J : . G K > J 9 = < E M 8 : 6 Z ; > D L N 1 O = E D ; 6 J 5 @ > E 1 ; ` > I O ] c \ [ F L W T P J X < ? < V U E P I Y G ` e Q C V A H L I N > ? E K > , 9 F J 5 E I 8 A K Z > = S Q K e j E W b [ n ] e e F P_) I ] v v k Y i \ V Y O R [ Q Q C S B ` J Z b _ T R K Q D J S C I N E Z X Q S S ^ N N S e d B Z Q k q )r?< /u) 0=|GQ[/ t Z l M Z ^ M X l E a \ Z Z G T I m ] _ h d x J8%|<MHC q x j g i u L i S ` [ d [ p b e e f R X R U L U P C L [ I K P J 4 ; K 7 9 . H E F Y J 9 G M C D ? = N 7 G @ 5 6 H A C ? Q ; = O E K W H @ 8 L < 8 2 T L F 1 9 O 9 2 < L P I K V W Q : K ? ? A N > > ? F R J V \ A N f 4 @ @ - ? 2 < G H 4 9 G E & 7 : 2 ? @ ? I ? K P = > > T L : 6 0 ( . < B A 1 2 M C @ F G H M < G H : 4 5 H 3 J > @ 8 3 * 8 6 C 0 K 7 7 : R < L 7 = B = F 8 D P L O ? K D A B * Q Q 0 9 : ? S & S Q 7 R T Y S ^ a h z 7,~252lZ+ z k ] M d J K J ; I M P U I * 7 9 8 = P @ E 4 J P D < A 7 H A L P G T J @ J 2 A Q ? D C ; 6 : < + F < O @ L P T - < I = M 5 / K 7 5 J < N V V K Z L U X T Q F D K J @ E ? T H E M C J A ? K [ ` _ t | F'( VCB ~ } w J h b P R R U F D p \ J X N = V F A 9 ? @ I K W @ H C C : G Q V D = 3 ; ; 7 A = I D E L _ W J U H 7 H 7 M B @ 8 P H J P K n ^ a _ ^ ^ W ^ d m H a L _ L E R b Y _ q
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6818bb42ce26445a878f9be8ae10aabcSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
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c "B.W V )BATTATTCAATCTCCCGAACAGAGAATATGCGGTGTTATTAAGAGGGATACCTCCGCATACACACACCGATCTGTCTATTATAACAGGATCAGAAGGAGCCGTACCAGGAAGCCGGCGGGAAAGACGGGCCGAGGCGACACCGGATTTCGCCAGAGACGAAGATACCAACAGAAGGATGGGTAGGGCGGGTGTATCCGGCATGATCGGAAGAAGAATCAAGAGGGATGAGCCCTCCAGCAAGTGACTTCGTATCAAAGGAAGAAATAGTCGGACTCATGGGAGACATTTCATTGAGTCGAGAGGAAGCAAAGATTAATTAAATATCAAGTTTCAATCGGGAAGAGATCTCTCATTGGGAAGACACTGTCATGGAAAGTCGGGATTATACCTTGACATACTCATGAGGAGAAGCGTACCCCAAGGAGCGGGTAATTAAAGTCAATTATATCTCAGATTAAGGAAGGGAAGATTCAAGGACCTTCAGAAGAAGGGGAGGGTAACAACAACCACGTACTAGTCGAGAAGCTATAAAAATTCAGAGGTAAATAATCGCACGAGGAACACTTCAGGATAAGGATATCGTTTAAGAGGATAGTTGACTCAGAGCGGGAGGATATATACACATACTCACACTTGTGAGTAATATATAAGCACGAGTATTATCGTATAACACGAAGAGGGGGAAGAGGTATATAATAGGTAGAATTATGACAATACTTTAATACTAATATATTCGCGTAGAGGGAGCAGCATTATGTACGCGGAGGAGACGCCTCCAATCAACAATAGCGGGTTTGAGAGCAACGATACCTACATATATATCCTGTGATGTAATAATATCTCCACATATCAAATGTGTGAGAGTACGTCTTCTCTCCGGGAAGGTATAGAGTGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 14:16:48 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA * U DATA
* U : DATA * U DATA * U DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ k LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @k PBAS < PCON Uk PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AYRM SPAC < SPAC AYRM SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH < phDY big phQL c phTR phTR w This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA * U DATA
* U : DATA * U DATA * U DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab O FVoc > FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ k LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @k PBAS < PCON Uk PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AYRM SPAC < SPAC AYRM SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH < phDY big phQL c phTR phTR Op]C$xXO+lkfc40-fKbVUg|=a1tirD:Z5A>9^kZdg~WI|Mb;eX}"if32#/P[~W!Fy^q3Th$Y i ^UO ]ddc}?8d)sGi=&MQJ1r]ZvZ~h6dYHa?yh;}S$PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AV^V4?HV;mUCBM[?|$}d& 'iJ4~CubW_]?ZC'T3T&#ONx8LkV}d7?#)io5`N0W`,#Xr_<.Fi1A+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\W\lWQ7M3gptQ>?";!8L[2Rr`qHT^1R=goQS-CDiyX^UM]N=?W.ss$T!D@4ir$Gj~`? MlYXjlnK[Yl`A #pJE,#VuU4zr4v4+AzM/.M{|Un*:WyF >0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#j1DjU5]DtmXY#agsC!.:QI5&.XRz+*}Lq&Wi1o9]^v*?u?&Mz*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz99#memIpp7pRma}Xc-l?55^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^ FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 2 LAST ~ ~ k LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS < P1AM _ P1RL X P1WD f P2AM t P2BA { P2RL m PBAS @k PBAS < PCON Uk PCON Q PDMF PDMF < PLOC J PLOC C PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% < SCAN 3 SMED
SMLt SMPL SPAC AYRM SPAC < SPAC AYRM SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE H8 Tmpr < User phAR phCH < phDY big phQL c phTR phTR