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7aQZ > z w u d v x e t } t m i j h h { ` W \ l i ] K _ d o m { u j ^ a j o i O W i @ ? < A 8 ` W 5 Q U D W 4 H 7 Q ? / B H % R B : J C P R S R \ q y q x u { ~ y z j [ ` R P X ` S Z B ? T F = B ? J @ C ; @ N D > D < 6 P J T Z < S d J > J X N G B 6 C G G N X ` L ^ [ N M Y e _ _ _ Z U x d t w /XQGh1 y ^ V a ^ S E Q a W g > O Q m _ i P O ] f X ] ^ c e ^ i K L B Y c Y e l e w r } QJ(p&_1 o d ` Q ] U [ @ P > I ? L N G 9 S B I R V V T N M _ H K ( > K @ L K B A B N E F ^ R I : K E R d a ^ Y U c e h U ] ^ D ] d ^ S [ H N F W J J < P U K ] Y [ M V R d U R O ? N W P V Z Y Y R H O R O U ] D I V ? O T Y k P \ N \ Z ` ; Y Z ^ Q U Z S d U \ I < J U : ? I H K K X F F B ` b @ C _ G L ` X S P J ` V V k q t p { e j v e f k s f g M X R b V T R K E S ^ \ b U P t c ` l l h x A| yZwaF "j& s z ~ Y a t S f Q W ] ] [ X _ b \ I R L N N F ? C 6 N U R ? T S > Q X C \ F T X M O D Q O 4 0 B J 8 : H F 9 6 E F H E < K U X E D M L C 6 R P G P H F C T F O J T O H b L G Z G T [ B I L X M H N Y L a ^ i ] V ` d m X R L L P B c / 6 G ^ Q < X F J S [ ` g l v u ~ s r x k } z 'cA'2A>'> Q( | r w a f ` = O Y R K L M R A h h S } R C f [ k d N h P k w t | j s t u ^ b R ^ M ^ Y X W : V S V O O P _ J @ N : = O 8 H J M ? 1 ? W > , C L H : T @ Q A I H @ D 3 O A U I < L A N V S = M I E J Z N i A R [ U W A > D R C ; > N M E ? ; H D = P A [ ; C > ? S I K M B Q T Q W X f a \ W O J L G 0 G O 9 I D ; O M H N I O @ X S G ` ^ p | J \%{7Sz`nW8Hy: l m v h Z _ ] S K P U L A / 9 G P @ 3 O A H N D R > = ? L K D D 9 C ; 2 7 / E 0 9 = 8 @ < D ? : F : 2 ) = 5 A B @ ? ? I O 4 : E - @ ; I E 5 < G 4 = ^ X e _ G D 9 J E J L P L D ; < J E 2 @ 3 6 I H 6 ? 9 M 6 D J H Z Q W T K A 9 K C D K K D I [ S > H @ = Q R F 3 B C F > = < 5 4 4 ; 5 5 4 4 C / F 7 ' H F Y H G h s M:B.m' g n b U Q P B 9 B M \ Y e a c h t u { ` k M H ; G ^ 3 F H L A H / C 6 + > ; - 8 A > = 2 + . 0 = & = / : = 3 & 7 < C < P E N M A : I @ [ ; B H > ? 1 F 6 @ J , . * ; : B B C O H F C 0 G B B C : P > I > 7 4 > ? E 8 . F B 9 6 G : 5 . I 9 / : 6 / + 2 2 / ! 3 * H 6 C < E @ L 4 . I H A 5 4 0 ] 8 : / 0 , * @ F 8 4 7 F : 6 > G S A G P J M A L J O F = B H ; A < E P ? U I K ; Q \ @ 2 2 4 - > W > 6 8 + : ? . 0 6 = * 2 6 ) = ) < 3 G : 9 9 C I 3 P 9 > O J ; ; 6 9 N E G D V _ U L X W q e k WKKy ^bM } m u u j K J E B 7 ; Q 5 8 0 + B + = A G E J > N F W K P ] [ a P Q a v a t q n q | r t h n N J Y G M @ 3 ; K ? = @ = A 2 4 G I : 8 , - @ 1 ; 4 6 . ( 0 6 ) 3 4 1 N 4 > / 3 & % * / - < : 1 F D : H B S ? \ I Y R d W _ Y d ] k v ~ r w k m Z g _ R j J L Z o c y d y c j o e q m n Z [ H P g ? F U ; T = G 0 ? 2 ? : E : : @ 1 D G D 8 = M U ? N N C H S C F Q M a _ Y V j t h [ i ` k A > ^ W g D I @ Q P P > < N 3 C I F I L Q L T 9 ; 6 ? D B F D P ? 1 F = 9 S = P D G F E U T O l a b w u g y ~ "m,L>
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1k F 1Vde}rYg/FV. y ` _ [ | t ] n T Q L f r Y W g W k e v o n #TSxO!sA ~ e y t _ i p b l _ F Q X : \ N U K V H ; B O D F H J N W O n S \ @ _ ^ g ^ j G L P M L g R M F T P _ ` A G N P J P L \ [ e n c c h Y g W R W Q C J a ] Q U e Z ^ J y } t u w x z YtgSpyll#O9q[}`\emmipQ t*vV6:*2Hk|l?' y x ] w h V l k q h q u l h n s } ~ 78 / Cv2gr*I { q Q ^ \ T K 7 > > ? K E K L Q [ Y ; L I S K < ; A @ B F : T H T P K F Y Q B @ L S g a V j L B S H Q N B A ? 6 ? I M B O B 4 D : B @ ? C ; H 9 9 ; . 3 7 1 K Q . D M : ? I A @ R H O < E W C 7 ; E O : 1 ; 2 , ; 6 < ? I 8 A I c 3 < D 6 8 D > 5 D H > B G 6 @ A 3 5 E : U D D 9 O J G S [ g F \ d N 6 E W L 5 5 E 5 H P > H G 6 ? A 1 - : G 6 D A 4 Y K < ; 2 5 5 J 2 B A 5 E F O K D 6 5 6 D D 4 ? A = 3 K Q @ E F F 9 ; G C 9 > : H L > W > G Z X N a b k c p O [ \ U R ` P U P S p ^ e j _ ^ W U _ ` M b G B 9 I A ; 9 6 L ; 5 B < L T C V I M i b r v { | z #Ht;Q@@RI | g n m z t } _ q l t h S d m N O U F U [ K P F I P L e S @ [ I K > U U > S c g ` T T W Y ] i _ t 6` _%JB:5bn7T-3-#G* Z h Y ^ N ] E c E g F N ] d V a [ h Q \ ` e ] k k W N U d \ [ q g ^ P u p :R@smP0 k- l Q L W O A J I > 4 C H B : ? Q @ 3 9 : 3 K C W \ Q K [ S e S m k u w q } Rr`Ukr[)[W !M!|B x w k a d D T A I A G C F @ 8 B E A F G M U N 9 M R A > < P B Y = Z F > K H Q Q R [ G [ T g \ P @ g ` m k w b u w u 2d)r#pqpp;7)]5[ | ~ ` _ [ e ] R d \ V ^ c m ] O S H Y [ M _ c e T g R K [ _ B ; G U M e U [ g J S O O < U V G ; / ? L W D E E @ = > ? / K 3 2 , 7 3 ? 6 K . 1 / 8 < : J $ 4 , ' @ ' 7 8 A 4 6 7 4 + 7 1 9 > , 5 3 4 B % . % = . > & - 7 # + ( G 4 6 / = 7 7 9 0 * 0 - & ) < - 4 - $ ( D : A > = ? 8 P G S ; 4 5 D 4 D ' 8 J . F - 6 / - 1 . * : / 4 7 5 > ) ' ? ) # 1 / ; 6 7 6 5 6 5 $ . 0 ? 6 9 @ 4 0 B C 7 ) - ; C . * 5 ' $ . ) , ' 2 & ) * " ) $ # 1 8 $ @ E 9 5 5 N H L T F , 7 F 0 2 + ? > = \ 6 8 < 1 N < I M < O U S k v x { s q z j m Y M ; < 1 2 7 5 4 . 6 9 : 3 ? 6 . : 1 0 9 E & 3 ) 5 ) " ' 9 / + F B d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333G>GRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR EWeijjkjjjihfdca_][YVTQNLIEC@=;8630. ` a ` V V V { { { ---999DDMPOZ[[eeeoooyyy
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 44c1ad3f8cdf41898f8a140ce116c6adSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
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7 " (- 62V<QD k `k* 0 h l l7k2'_L6L BATTTTTAACACTCGACGGGGGAGAATATACACTGTTATTAAGAGGGATACCTCCACATACACCGAGGTAGCTGGCCATTATAAAAGGAGCATAAGGAACCGTAGAAGGCTTCACAACGTCAAGACGTTCGGGAATGACACCTTACCTCTCAAGACACGGATTCACTCTTGGCATTATTGGTACCTCTGGTGCCGAGCTCCTTCTTAGAGTGATCTGGAGGAGGGGAGGACCCTCCATCAAGTGGCTTCCCAACACAGAAGGAGTGAGTCCCTCTCCAGGGAGACCATTACCGGAGTCGAGAGAGGGCCTACAGGAGTGAACTAACGCGATTCCATCCTGGAGAGATGGCAGAGTATGAAGATCCCGACATTTAAGGAGGGTAGTATACCTTCAAACAATCGTCATGATTTAAGGAAGAACTTGTTTTCCTGGATTCCCTCAATTAGGAACCACAGGATTTATGGATATATCCAAACGGTTCGGCGATACATTGAGGTAAACACCCTTGACAAGTATTTATTCCGTCACGATAAAAAATACCGCTATGGAGGTATACCGTAAAAGGTTGGAGTATACTGGTTAAAGAGTTACCTTATGCGGATAATTAAGAATGAGGTTTCCGTTATTTAATTATACTCACGTATCAGTATAATATAGAGGTGGGTTTTGTATCGTATAGTTGCCCCGCTCGGGCAACTACTCCGTAGGAGTATTCATATACTTCTAATACTACTCTCATAGAGGGAGCCACATGAGTATGCCGAGGAGACGCCTTCCTCCAACTATCACATAATGAGTGCATCGCTATCTCTATGTATATGTTATATTATAAGAATACACGTCACACAGTCTCTGGGCGACAGTAAGTCTTCTCCCCTGAGGTTATAAAGTAAGTATGAGATCCTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 14:16:53 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +` V DATA
+` V ; DATA +` V @ DATA +` V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 7 LAST ~ ~ L LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS ? P1AM ci P1RL \S P1WD j P2AM x P2BA P2RL q PBAS C PBAS ? PCON X PCON U= PDMF PDMF ? PLOC N' PLOC G PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% ? SCAN 3 SMED
SMLt SMPL SPAC AXX` SPAC ? SPAC AXX` SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE E7 Tmpr < User phAR phCH ? phDY big phQL c phTR phTR v This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA +` V DATA
+` V ; DATA +` V @ DATA +` V DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 0 FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 7 LAST ~ ~ L LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS ? P1AM ci P1RL \S P1WD j P2AM x P2BA P2RL q PBAS C PBAS ? PCON X PCON U= PDMF PDMF ? PLOC N' PLOC G PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% ? SCAN 3 SMED
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