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bcd0o= v p h S I K L U W J N ) 5 G H = 7 J H H 3 1 M F @ @ 7 = 4 G G Q K E 8 3 1 E 4 = > @ J C ^ E H @ ? Q ; Q N C I V ? @ B @ M @ O G > @ A 7 S : P L A @ B 3 8 @ = @ ; 3 < ) 8 A 4 0 9 P G B 1 N L 2 4 8 5 7 9 F J < > = < D I K 5 L L E 3 P 6 N E ; 3 D 1 J O D X [ ` N K H Q Q O B S J K P V Y ] d X ` g a X ] h M _ z v )4WG|RpvE">UJ^'G" w ` k n f H W [ p ] P X Z e ] W T H J M U ] U L W M S U a Q f d ] [ ` T k I P b f d K C K w d Y c Y L N Q R p R [ _ i V _ T \ i T W d r Z h d d p w *pI y v _ Z J S F H ` L G 7 B O E P @ O 6 : G U ? G P F P 6 H ? @ 5 Y S z 1
{ d ` X _ U h S U > 3 C I N P Z : ? C . K J E L C 8 D K > S J I @ @ B . H = E ; 4 @ 8 8 < 8 6 G 8 < 4 = P C H Q J Q = P X P 7 F : 6 D 7 @ > A A C C Q I _ \ j \kd ` c X V T I K < A 9 9 E E - @ 2 F 7 J C 4 G ; 9 @ ; M ; F 1 . I > K . N B J 0 5 0 4 8 . E 0 D 8 & : A 4 ? N + ; > , 1 G " + 8 J D % K 6 5 1 C ; : H 7 O , 5 4 7 * 5 5 ' 0 D ; 8 , - 5 = 1 9 $ / 3 ? > F @ > C A 2 $ C D 9 ? : F ; 7 ' F B ; 6 I 8 ? B I Z Z u ^,40= m e e g J S b C I = B Q F T M D I G @ A J C 7 K J E = W ? I [ U S b R V @ R K 3 ; 6 9 9 3 A 6 G 8 E G P D @ 2 E e F W P [ T P > C H T M E I E ] P j h h r `i u n T W ^ c Q d l ` [ T E : = Q W ` w #WqS`Mp 2i7 i z w w i y qV$ o d _ W N J N ; ? @ V ? @ / O I I H O G E R C R R G J R F I Y P X G U P Q 3 J @ _ Q P S Y T L N X g ~ s >ujYaTm =kIr( o g ] T ] Z Y Z P T F K J b ? I \ X [ b b } "} 'g6 l d L O K R = H T O O K E B S F G L O < L 1 F Q L J R ; 3 4 < 6 = 5 9 C 3 B - * G D / 8 C : I ; 7 B @ 8 O F L 4 C ? N U D Q P G K S O H : C P N P U D C J S H U L D g f (y9 x w a Z d W J P m X U R Y D g Y ` L X H X V e K W X a X J c O _ ` P a d Q Y a V Z a j X { 'aS% } i c _ [ a c t V ` u \ ] o t V c i ^ i ] b t o c s o h u P q n m d f v r g } l 8i\& u m c f k } q )2 q o p a g i K g S T f U P _ Q M G V S K e d { %Li } m y ] Y Q W L O J = : C F D U G O d ^ *nf z k d j c E R N = : 2 P 9 8 > > J I 6 > F D < C I 9 6 R 0 J K M 5 9 9 1 A < R A @ P U I U B 5 @ B H 9 E 7 N > B E T : C N > F Y L 8 N Y ^ S \ Q X U [ a Z N X V Z M P Z [ k W > X S P L C 9 ? S J E Q K e 7`x\ y y j p Q ^ m Y K L I 7 M F < @ 7 = @ L ? 5 = G < H P V F N M C F L A \ V L A : F F 0 W = = ? > 7 : = @ 3 = ^ T : G T S R @ U V ; M g O Y ] _ q f Ism~|ulg?
y ` U u N Y c R W B U J B G 3 D S 8 H X H A K G 9 < C ? ) / > 8 G A C : H 4 A E O < L D 8 $ M 6 D N R < L D A : : O N C ) + 5 ? = 6 9 @ 0 > 2 Z 6 3 1 > 9 9 3 7 ' 3 * . 8 4 ? 1 : / C [ L 7 + X [ 4 8 M M 9 F G ? I . V 9 > - 6 6 : 8 I , E 9 E @ I ? 8 H @ A * G ? L = H j a a z y 1Rqg. y f V l o y | r _~; z q f c T R Z L D B ` X G M X I H K L J ; J \ < C O V I W J C H ] Y { | +,Qdth_. k p \ B > A 3 < G 5 3 2 < J I 7 P > 8 7 9 N D P : > > @ 7 = U @ B 1 3 > 4 9 . - M 8 0 2 I @ > @ G E > C 7 A 6 ; : ) 9 S : 6 / 7 8 G F 8 + > 8 ; C 6 . 5 L F B ; B / H C F 9 a 8 A K ; E N Y d y _ } u ~ D/cwbTJ# m l a T m Y _ \ Q Y \ ` Z Z [ b k i b y D|LcqvW\Fbq^O< y m m X M R Q F E O > A H W @ 1 T 7 8 D > * ? = 5 E D 9 G @ @ 4 H 8 ? @ H @ I > J Q H 7 C 1 : J > 2 * ; C U H > . 8 5 * G ; 0 * = J 6 5 9 @ < 7 & - 8 @ 2 1 H / A < 4 : + E > > 8 C , C B A : A L < I I 7 B A B E 0 K C = 5 8 O L ; J Q > H < H C 8 J O O T U H M Y O : Q @ C [ g x x \ ] G A G H F I I 9 - / ; < ; @ / : 7 J @ - 1 8 ? N ; 9 - , 7 : 8 ; / , 6 7 3 - 5 A 7 5 / C F 7 ; 2 & ' 1 ) " . / C B + 4 . 9 & 7 1 : 5 ; < 9 ; 1 - H N 1 D 7 - 7 ( / + 9 / / 3 ? 7 3 ( 2 < 2 . ) 0 / 0 9 0 + * : ' 4 ( 8 ? ) % < # 7 < 7 * ' / 0 $ 1 6 + 3 0 ! . 0 A 4 2 < 7 > # - 6 , E & 6 = 4 , 6 4 0 . < 0 ' > 6 6 < 6 < & . & 3 ? 9 . 3 1 7 ! < ? E 4 5 ) = 6 * @ . 3 < 6 - : 5 1 4 7 2 / 1 / > 0 - , 4 % " , 2 ' 5 " / 0 & * 0 ' 5 / 6 + ) 8 5 0 6 < / $ ; $ ? < ! ? D N I 9 5 ) 3 7 H * C ? 9 J B C _ | i *RY|v`c5 r k o L b R L = : C F : B > ) 2 / F . & $ B @ < ? & 1 ) ; 5 Q # + , B & J ) J ; : / * 1 * P 9 4 ( 5 1 5 6 2 9 2 1 4 1 ) # - ) 3 < , 0 8 9 C 8 % , - 4 - 4 5 ( E . # & 6 6 / G ) < > D 2 < 7 1 0 8 9 & C 2 0 4 . % > / + . + 4 6 3 9 ( - , A @ * 2 7 * 2 6 . % A + ' 1 7 * 8 3 , @ . : 4 + . 5 5 4 @ 8 - @ ( 8 + 6 / 0 . 0 1 6 * 5 C 7 . = K ; H C . Z : ? ? H [ 2 D K Q G b r "/2b\1+ ~ Z ] O K A O < 3 4 5 7 0 ; / 1 C 8 7 8 8 / 3 = 6 / < . J @ 8 O J 5 < 8 9 ? 7 I A * & / * P 8 E 8 - / 9 ; : E G T c p "@{qk]]vy|ZgZLi]Qew~g^c=4 i ^ ] V S J W ? C 7 C B A P P . R 8 F G ] Q ; ? J F @ S ] X Z E]ywj6 q s \ U U K G E E S E T M I E H M N < F ? > F 1 > 0 9 > E ) 6 5 ? N % / 1 ? 6 A J D D I B 7 ; ? @ M U U c Z { 4TTnuX</ q ] b n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e ~ OYWx_b- h h Q ] ? : J 0 8 S : 8 . E . < 3 & 4 1 3 B - I 5 D C = U . D L r
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3c2ba432aeb64357a69e6f2a41f02c52Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
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hFGGTTTATTTTATTAAGTGAAAGGGAGACAGACACTATTATTAAGAGGGATACCTCCACACATGCGAACCTATTGGAACCTTATAATAGGAACCGAAGGGATCGTACCCAGCGTCACTCGCTAAAGGCGGTTCTAGATGAGTTTTTAAGCCTCCAGTCATCGAGTAACCCCGAGAGGGATTAGTTAGAAGGTTACCTCTCCAAAAATGAAATGAATCTTCCAGAGGGAAGGAATGTATAATAGGAGGGGACCCAACGGCGGGATAAATCCTCGCTCACGTGGAGCAGAATTCCCGGGAAAGGAAGGGGCAGGGGCCTAGCTGCATATAAAGCTTCCCCTCCGGAGACAACAGGAAAGTTTAAGATACCTTCAGTTAATGATGGAAATATAACTTTAAAACCTCATTTGGAGGTGTCCTTTAAATTTTAAGGGGAAATACCCTTTAGTATCTATCTCACGAAGGGAGGGAGTAGAGCACTACCTACGGAGAGGGGGGAGGGTATCAGGTCCCCGCTCTTCGTCGATACCCTATACCCTTTCAGAGGTATTTACTCCAACGAGGAACGCCTTACGATAGAATTAGCATCTAAGAGGATAGTGGATGCATATTGGAAGTATACAGACTTTCACTCATATTTGTGAGTAATATATATGTATGAATATTATCCCTCAACCTCACGCGATGGGGGAGGTGAGGAAAAGCCACCGCTTGAGGCTCTTTGAGTATGACTATTCTCATATGGGATACTCACTATTTGTGCTGAGAAAGGATGTCGTGTATTGCACCGCGGCCGGGTGGAAGGGTACCTCAATATGGATCCTGCAATGTAATGGCATCTGGTATATATGATATGTATACCATATAATCTTCTCCGCCGACCGGTATTGTAGTGAGTAGAGATCGTATGATAAAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 14:16:55 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt o ASPt o B1Pt n B1Pt n BCTS } BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + WD DATA
+ WD < DATA + WD H DATA + WD DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS A P1AM & e P1RL & ^ P1WD & l P2AM & {; P2BA a P2RL & t PBAS E PBAS B PCON [ PCON W} PDMF PDMF A PLOC & PW PLOC & I1 PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL SPAC AW( SPAC A SPAC AW( SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH B phDY big phQL c phTR phTR w This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt o ASPt o B1Pt n B1Pt n BCTS } BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + WD DATA
+ WD < DATA + WD H DATA + WD DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS A P1AM & e P1RL & ^ P1WD & l P2AM & {; P2BA a P2RL & t PBAS E PBAS B PCON [ PCON W} PDMF PDMF A PLOC & PW PLOC & I1 PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL SPAC AW( SPAC A SPAC AW( SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH B phDY big phQL c phTR phTR k#[Nn$Q{2CWllm4y[*M0Jhjag!aczjCt>-8G8A,Fl.XOAGi[UePd7n%&/VmO\rwHB*@Y%H_TQFGa^_#=?o'%x'[vA-\K"L1{!UlyN7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3XwC^8m]Cy4=?6vtfQgsk>H~pL"A~oTq}}89u:KQwMx_R,QDb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TZc:'?tVb"(\vO+hPAFK9p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$5t{`n&|gp^V"YZqsC(Y5_}4]'o>s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4 AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt o ASPt o AUDT d d B1Pt n B1Pt n BCTS } BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + WD DATA
+ WD < DATA + WD H DATA + WD DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS A P1AM & e P1RL & ^ P1WD & l P2AM & {; P2BA a P2RL & t PBAS E PBAS B PCON [ PCON W} PDMF PDMF A PLOC & PW PLOC & I1 PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% A SCAN 3 SMED
SMLt SMPL SPAC AW( SPAC A SPAC AW( SVER 4.0 SVER r SVER , , Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH B phDY big phQL c phTR phTR