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e d Z i N N X H Q H R = ; B Q J E N V A Q 8 ' J M T 8 H A C C 9 : < ] S 9 J A > P 8 B J H K G H 0 B H B < A 2 A 2 @ I < < L ? @ ; , O > = ; I ? 4 + B ? ' 1 7 ' 8 @ < 6 8 R & > 3 = : + 0 = 5 B - 4 D 4 = - 1 I ! 1 " @ 7 1 1 8 5 3 3 6 6 ? / : / % 2 ) . ( 4 ' @ = 0 ! % 8 % & 0 ) D ! 1 $ 9 ; 3 0 E B < 9 A D @ K 3 9 P E - = 2 / 5 # 7 7 / @ * ) ) , < / 7 ; 5 3 2 I / = ? 6 0 2 4 8 : A < 4 5 I * 2 @ % D 2 < G 0 L P N [ C _ F f Y Y S d T b L K T V H B a g ` z w l &]0GPK(t; s v k a R ^ e _ c W c q Y o k h q y n o | -s ]2,6y> y } G D L W < K H Q \ I U K P a X O [ H U h { } f f c h q P ^ R f Y f o p W } x CA@TN x n m \ c e S \ N Y [ Z n c b ^ ] d ] E W ^ h ] f h e h o l p w q*Cek\J>^= N 6UJQ0v7 t l c U S C Y 8 L F _ B 6 8 [ J N W ? W ; : * B * C 3 3 / , ? - ; ? D : 7 9 ; J A 7 T 4 E C 3 > = @ ' : 4 - 8 ? - + = @ ? C 8 4 $ 9 - 6 1 & 0 + + " - . , ; 1 - ) L . . I F > 8 2 % ; " 3 + , G > 8 1 4 J > @ B < F K ? Q X \ _ g Z k x h q p q l r s j 9X),PW oP o c S I S T S W M D I M N , ; > ; 3 5 @ + 3 O 8 I = = 0 0 $ , J ; B , $ E < ' ( B ( ; 1 + + 4 C 8 = 1 D @ 0 3 * . : @ @ 5 9 0 7 2 & * 2 0 , 5 + ' / * 7 / # . ' ( ' ( : 3 # + 1 5 > B 8 0 G 9 + E $ , ' ' " ' . ) < 2 ! * 0 6 1 $ % : 0 , 6 * + - * 0 . ) ) ( , ' 4 ' + # % / + - / ) . A $ + . ! B ! & > $ 4 + 1 2 2 1 7 - 4 $ F 8 3 8 ? 6 : G 3 ; A K ? - 3 ' 2 K 0 ; ? @ C - 1 6 = B 7 3 5 > : 0 R L I F N E G L ] N a U < E S P O W T S ` _ t { { Jp=KIi]* f-* &c)k~~]tQ t i v v u q v ^ v s j/lv{J,m?; 5?Wu %(Ws+:D63/
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S# z u V R f X 9 E J D > 3 L C A A 9 7 D < 6 : 1 H 5 G N : 0 8 H 7 + = E N 3 6 < 3 0 4 D ? 2 . = M S P L k D h \ [ i Z ^ | { Y%Me? [P t q q i b D F b I H E 4 < ; + 4 3 8 7 / D , 6 4 3 A ? A G < / @ O 8 9 C < 6 / L D = . D 5 7 ' 6 0 8 % 7 3 , & ( 3 M 4 , < - > + 0 ' / ! $ + 0 5 ( / 5 2 - 4 5 < 5 7 9 ) ? 7 8 . 7 * 6 3 $ * ! 2 7 $ , 4 0 7 / ; ! 6 1 8 % + . ) @ 5 # ) ) ' ( & & 6 + " 6 3 4 < $ ; . - : 4 / ! % 9 / 2 ; / 0 ) ? 6 " # 4 ; 4 - , D 0 @ + + 2 & ' 4 * : . + ( + . 3 1 ( ' 1 ? ) 1 - ) ) , 3 6 @ C - = C 6 9 @ 3 < 6 / + ( G L E , ; 7 F D D H C 6 I F L : 6 4 < B H @ 5 > 5 H K 7 E U H C \ O B I G < D M N G J P _ G K E : J G O T V d _ x n v 'O $! D r v e T ^ [ E N M K = 7 8 E @ 9 5 K O B ; Q O K N L E K E Q U T L ; C > V K \ D l w R _ T _ d q k k | { '3qcxgZ@+ IZez^'+ x Y S M S @ B Q ? I 6 , 6 8 4 < 4 2 1 8 > * . ' 8 8 < , F @ ; @ 5 < 2 > , 5 / = D + G 2 3 5 P " " * , # 3 $ 5 + 9 @ . 7 ) < 8 < 5 - 9 ) . , : # 5 / ) 2 4 1 & # ) , ) 9 * 3 ) ' F 0 5 2 5 7 # < - . $ 5 ' ) ) 2 9 * ' & ! ) , 4 9 , . 9 1 # 1 ? / B 2 F . . : = 6 - 3 . 9 8 . , > 7 5 & ; > - ) A @ ; ? , , I M 9 A J > < H 5 8 E Q = M H E > E J A 6 B 7 I R O \ M E S M _ y w 9Ym ~S/ s i u v c F
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jeRTQQw nv:B p k l K P D E C P J < , / ; L 9 G > < N A B . = . 2 ; 2 8 : = 9 E B G 3 L B 0 & : ; 4 2 7 0 E 9 8 + . 0 I - R B : 4 2 + 6 2 B * . ? B % F C ( 3 , 7 5 7 ( ; < & 2 ! $ . / 4 ( 8 8 ) * 1 / + 1 ( 8 ) * + + * % " ( $ ) $ ! ' ? ! ( ( ! ) " 0 * 1 $ . & . 2 - . * + % 7 . ) 1 < : / > 5 " 6 < / % 6 9 7 ( 7 D J / . ( " @ + $ ' ; D ? < ( - + - E 3 & ; 1 % 5 2 " 6 3 ' 2 " 0 ! 3 : 3 8 , / # - $ ) 4 & , 3 8 ! , * ( ' - < % # / ! + 8 + + 7 4 ' + ? 0 < 2 7 G Z = V O X ] ? Y \ M V P ] R F Q E X b c _ R c W t t u x i b L W R U L 3 + d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333G>GRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR EWeijjkjjjihfdca_][YVTQNLIEC@=;8630. ` a ` V V V { { { ---999DDMPOZ[[eeeoooyyy
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fcd4c46c7ebf45f2ab43ffd307d45eafSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_12-45_0553 3130Seq 12281 12281_Costion jmadera 96-Well
K1359_C8_RKB_3730_POP7_BDTv3.mobSeqPop7z Ai'Ps;`&7><4,&',363(|aOHILNKA3#
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tO9 L AAAATTTTACAACTCCCAAGCAGAGAATAGGAGATATTAGTAAGAGAGTTATCTAATCCTCGGCGTGGTGTGCTGAGTGGGAGGATGTGGGAAGGATCCATCATTGACAGCATAGGTATTGTAAGGCCTGCTTGGATGACTTGGTCGGGCTGAAGCAACTGATCTATTAATTGACTTGGTGGCAGTAATCGTATCACGCTCCAAAAGAGGATGGCCTTTAAGAGTTGTTTCCAATACTTTCGGCTTCTTCCCTTTGAGGGAATAACCCAACTCTTAGATTCTTGCCCTTGCTTGCTAACGGGAGGGTGCTAAGATGGGAAAAATAGGAAGCTCCCGGGTAAAAGACAACCCACTCTGGTGACTCCGCTTCACTGAAATATGATATAATAACTTTACGGGCTCCTCGGGGAACGTGTAGTCAAAGGATAGAGGGAATAAACTCCCTTATCTATTACATTAAGGAAGGGAATGTTGGATGAGCATTAGATTGAAGGAAGGTTATTCAGAACGGAGTCCTATACCGGGTGGTCTACAAACTCAGAGTTAGTTACTTGATTGGTTGACACTTCAGGATAAGATTAGTATTTATGAGGTTAACTGCCACAGAGGGCGAGGGATAACTACTCCTACTCACCTTTCTGAGTAATATAGAAGGAGAAGGCTTATCGTATAATACGAAAAGGGGGTATAGCCCTTCCCCCGGGTAGAATTATGACAATACTTCTAATACTATACTCATGGAGGGAGATTCATTATGTAAGGGAAGAAGATGTCTCCCTCATACTATATCGGAATGAGAGTCTCGATACCTACATATATAGACTGGAATATACGTATACACGACACACATTTAAATGTATATAGTTCTCCCTTCTCCCCGGGGAAGTTATAGAGTAAGTAGAGATGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 14:16:58 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS u BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V( DATA
+ V( : DATA + V( DATA + V( 8 DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS =h P1AM a P1RL Y P1WD h P2AM vK P2BA }a P2RL o5 PBAS A& PBAS = PCON Vh PCON R PDMF PDMF = PLOC K PLOC D PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% =` SCAN 3 SMED
SMLt SMPL SPAC AY^9 SPAC =x SPAC AY^9 SVER 4.0 SVER j SVER , , Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH = phDY big phQL c phTR phTR ~ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS u BufT ' N G CTID # CTNM ) CTOw 7 CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA + V( DATA
+ V( : DATA + V( DATA + V( 8 DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab FVoc FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE < LAST ~ ~ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS =h P1AM a P1RL Y P1WD h P2AM vK P2BA }a P2RL o5 PBAS A& PBAS = PCON Vh PCON R PDMF PDMF = PLOC K PLOC D PSZE ` PTYP ? PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT -! RUNT 6 RUNT
RUNT , Rate RunN " " S/N% =` SCAN 3 SMED
SMLt SMPL SPAC AY^9 SPAC =x SPAC AY^9 SVER 4.0 SVER j SVER , , Scal @ Scan 3 TUBE C8 Tmpr < User phAR phCH = phDY big phQL c phTR phTR kl{Zq\-4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6e-I*(~%5?b,iG:+vB`hCCW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-[J$I;fLQ:4<:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NDj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0