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(          $   "                          ! !           &%         (   N",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR?\cgggggefdb`_]\ZXUSPNKIFC@>;8641.,t```VVVzzz,,,888CCCONNZYYdddnnnxww                                                                             <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9153a8fa2d34414795ceb658f71ed8d3Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_01-56_05493130Seq1224912249_Costion_K1357_matK_Rjmadera96-Well K1357_H7_RKB_3730_POP7_BDTv3.mobSeqPop7z ,Dg0Lr!>Wr/h!4MjrZ?"iAnG#kXH<2*" 1G`z$Dawxl]J0lI'hXRRYcmv|udN8$ArO3\xt^?T K5Rst\B$skgffd`ZTQSYesvmgcchq}3Ol ?qv=s8}rh_ZXY[ake=B=Ij"}? r_K:,%#(3BTi 2RivythXE1teXOKN[w@&*Ox|]e:f1sV=')4<BEGGGEC?8/$ )BfJ]2'6{K<Lroe}!&N)9w>K1 +kD& !    (6BHHB9.% .e9IobT=-GH-k!{W9"       &Q[c1xYB1&!  "F{ Pa@tUE<83-% 1U@vHEycSH@82+%  !Ds )99*vV=.%"!    F;xm}\c)pK/W1"gkjQ(vRC@@>7,!'2:?>8.# #Ev;rh0wK,  "2Jj%NhqiS3qM-  $((# @_Ez=x7 $+01.)#U+T=mg/^mj9 1o0 _z9}U:.)'#   "RF~0V)  %%!    ,Fd#(kRFBDGHFA;658?GPY`egfaYNB5*          %*)$          $((#                                                                                 !6L`t<bI+c+U~6g @`}zZ0]+pD".685+[#g%r> {T7%  +AVj| -I_nvywpg^WQMG<)qbZWZdw%eFB=_tyoT*w:x} @pc*X )Gcv}tibacd`S>$ pG!Y1 .MyI3r  ^.EsO- }rkfb_]\[YUOHA<:99840*&"  '5Je'Y  pT?7=SzEXHso= Ld.sbTF:1)%#%'+05=GWn&^tIuWA4/158974101589840+'%#   !#$%$     &))(&%&)/5:<93* $6WoQpK3))1<GPTSMC6(  "'+..-/>aan2m+[6!"&+/22/' %*-,($ $*02/'     3Ox-fzKnE'0mP&23L}X>-"_R4THBoYPMIA4% Hw 4ub`fowyuj\NB<=GZy6~:ThC/('(($  ")--+'"  -45/$  $(,/121-& 2U\?*'>Yvy]D0$!$)-+% %*)#   *<IPOG:-"0k"$DE0TxXA0$  I je0 .-e3 |F %--'=xh\fJ9.'" '4H`|];  M<hOIVpx]LK]JogaWI<7@Y{%R%`4c ! +Px 2k!k o=|eG(>`zz_<8a~[6                   -680#                  %% (Wh8                                            "! -<GKG;,   (3=FOXbly9Uq!R-Qy <m%:Rk(B[t+@Rcsa-w1@?rb_fu 0W-FQRNJJQ]kv~|rcO8_&n-b"{i[OE=769?GOSSL@/I|>37(LelWJA<8533578860*$!#%)/5:@DJQZdntvtpmkmptx|$U;r'Lp*d4h^;}Ao`-l0~tomje^UKB;520-+)((*-1563+!<k<SxxW(q6zmaVJ?4+# '>`w>rnNbMhI0 $&# Fie7/MyO*4l,qc~V0[ oq<H;P[6  F355FoN8'&Lsy,Z8" )Gi+]:0koYJ=1%  3QonP2 7zL9W'W9)%&()'" 2hEy:{}P4$  #"  Awx$\wv[/vgW@ 1E\ Ti:&.21-)&$!"YRz>Jzi]_xr`Wq {}aMw|[ctC" *:FJG=0$&3AMSRI:(7bFs`7 oWE7-),8Njr [wl;b*  #$ J?6sN7,'$   !(*))3O)!g1coI7233.#  +a t JL&s< =y_TM&  2XUA@ +,vA!"=e=tx ,jRD8,                                                      !                                                                      "$$"  #&)*++,-./1233211113579::986554445544431/-+)('&%$"   --.28AJRW\ahq|2Li!Ee(Ig~qdWI;-|eL0xW;" }uqpqsuwxzvkc\VOG>61/037:<<<;<<=<;;:;<=<:74100/-*%" &,03431.*&"    !""! "$')+,++***+*($   %)+-.-*&!#&)+.4<FR]flopolid_YTOJFC?<989=CLWi)H-IJ27b+lUB4*$     ?sp>,HTq^OB6+!  ,;HOND4")5>CB=6/*)+058:9852/,(#  O!X"^ jL8,&$#$%&()+*(#0CUafcYJ:,# !&,1455444552.' "(,--*&! XLDT;J{oo6k`^kpczrkn&80oyV2D/'X yk]QF<2(   "()$ "((! $g[MZ-WXb='!%'&" &-/.*%""%+4=EIG>0        Jqk]|iWOKF=2% (YT i=cNDAA?8." @5&Oa^K- p0tM5*'((&         *597/"     #As i/5@qI/!         *;ISXYUOF=4.*,1:EPX[XPB3$                                                                                           <@ꅈAAMc AlKB.bcpKB_3730_POP7_BDTv3.mobtermGAATGACTTAGAGATAAGAGTGTCAGATGCATTGGGGTCTATTGGATCTCCTCCAGATGATGACGATTGGTGTCTACAGTCCTGAATGAATTGATCGTAAATTATGAAACGTGCGCACTCAAGGAATCCGTTTTTCAACAGGGGCCCTCTCTTGTTATGATTGCTCTCGATTAGTCTCGGCTCCCAAAAATGGCTCAACTAGCTGGCAATAATCCTCCATTAGTACTGGAACGAGTGATGATACGAATGACTTAGAGATAAGAGTGTCAGATGCATTGGGGTCTATTGGATCTCCTCCAGATGATGACGATTGGTGTCTACAGTCCTGAATGAATTGATCGTAAATTATGAAACGTGCGCACTCAAGGAATCCGTTTTTCAACAGGGGCCCTCTCTTGTTATGATTGCTCTCGATTAGTCTCGGCTCCCAAAAATGGCTCAACTAGCTGGCAATAATCCTCCATTAGTACTGGAACGAGTGATGATAC/=D[eku%/>PZhr%/9GUdov !,6BMVn|%18CP[cmy#4?Tbnz$1>HUco{ #,6CL[hrz#/6DMYiy    " 0 : C N W c n z   # / 6 B N X b n |    ! , 7/=D[eku%/>PZhr%/9GUdov !,6BMVn|%18CP[cmy#4?Tbnz$1>HUco{ #,6CL[hrz#/6DMYiy    " 0 : C N W c n z   # / 6 B N X b n |    ! , 7  !   .::::' 2:-):*21' #&> -!"!>%%  $#2#,$!>3'3&+"%       "    (                         !   .::::' 2:-):*21' #&> -!"!>%%  $#2#,$!>3'3&+"%       "    (                       $S WQ(9A*S"VP XK[SaXAbIR:H ,[XW ^GRaZS][]3LZ^@a. ?KH^Z !U, !CL %aPcZc?KAX.UY7,VHJV:;V 9X* :_ [O [4Xb bL N]VC/bX+ N]\A^H!^2J;[9[K( Y C2!2K', 6CSMZR<?*T"J SZ2dYM3W-2OJ-O ]5@G_}FR?*xE| j9c' z> ^AHw4;.,?';  ,N8/FYWj;5 g_Fw&'y1{8NR`fl Q^D.naqU<"H;gOrR> ]wDd:{,+TQ<H;.kgD $ j SO(]q>+zrjmxYbR{zod\ujWz_Nh~lje^QWmamwRcy^mbG`ELEYwj \;A  > 0 E-oWg O0<f0}&Mfp pppfp:#-p*~,!L8/FROn#q[?a:spaS3Xi,AjGu3gLh&Hkt8,Bd~V8L| dH(?[,2@\J&>@eVXAVjQ-Rybp;70]3e93iK&vSF ;@3N1-*:'8aBP@Nv1jJSmgEi8Ae2daNb3n}29 }zuz]c,)Z3WqD=l.*2oE\W(IATTATTAGATTTATAGGATTCTGACATACGCAATCCTGTCTACCATATCATCTTCATAATAAGAATACTTGTGTAGGCAGTTCATGAAGTAAATAATGTCTAAGAAGTGTAACATTGAATGGTTTGAAGTAACCGAGGTGCTTAGGGGACGCCCGATGCTAAAAAGGTAGAGTAAAGATCACTTTCTGCCCACTGTCTTCTTTGAAGGGGGCGTTATTTCGTTATGGCTCCGCCCAACCAGATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 03:27:21 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSCBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA #tFDATA #tF+DATA #tFrDATA #tF{DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISkP1AM &P1RL>P1WD P2AMP2BAP2RL PBASPBASPCONJPCONVPDMFPDMFPLOCnPLOCPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%cSCAN3SMED SMLtSMPL SPACMSPAC{SPACMSVER4.0SVER 8SVER,,Scal@Scan3TUBEH7Tmpr<UserphARphCHphDYbigphQLcphTRphTRgThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSCBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA #tFDATA #tF+DATA #tFrDATA #tF{DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab"FVoc"FWO_GATCFeat.."^GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISkP1AM &P1RL>P1WD P2AMP2BAP2RL PBASPBASPCONJPCONVPDMFPDMFPLOCnPLOCPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%cSCAN3SMED SMLtSMPL SPACMSPAC{SPACMSVER4.0SVER 8SVER,,Scal@Scan3TUBEH7Tmpr<UserphARphCHphDYbigphQLcphTRphTRGP58Jr7y!{_a1z{,c]n{uH<6#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`U*{Zq9FyhCebT8Poo=[/19~{2A~&!sR(&^iCq=dGE^E:DH6[~=7?xh?'kT'_C:q|4G,fX-N&Wv'i2{M~V9:%b?AMw35|MkHIt+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4g.W3DK]T5^.?s|"Ff61}XTu.ycI0 r#U4=YnJ<:&ue\sKZI\T(k"T'o)6yHAj}=P1WD P2AMP2BAP2RL PBASPBASPCONJPCONVPDMFPDMFPLOCnPLOCPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%cSCAN3SMED SMLtSMPL SPACMSPAC{SPACMSVER4.0SVER 8SVER,,Scal@Scan3TUBEH7Tmpr<UserphARphCHphDYbigphQLcphTRphTR