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N",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR?\cgggggefdb`_]\ZXUSPNKIFC@>;8641.,t```VVVzzz,,,888CCCONNZYYdddnnnxww                                                                             <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a14e1d5da2c141a9912d9695241cb4e4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_01-56_05493130Seq1224912249_Costion_K1357_matK_Rjmadera96-Well K1357_E4_RKB_3730_POP7_BDTv3.mobSeqPop7zy`C,#-K|5{M  Ifyg&U&Ffywj`hY! ,U|2;,{.cj%CU=9gymL$$=KF+(EF+logQOZfgYA* '<Xq~kP23Unud= )u?.HZ\SGBDC5R4).=RiBe{xhYTYbf[=O:^Y2W|hbloN3!/MrrH /BH?*DzwBP"\@?_yz1\|h){4#?c7eu63A>/(+,D@kNJYnzvaC% 8WzeG1" 9b*Gge$A(`3QO8 !Adb%W/%;Z|#&"%2DUew|)r1*Rzh?:MM; (Uh2 =wp< %" MVr-laq&UU i8(6Vud>  #7` v(*ec4 4EH: &Jfm[: #7=6% &6BD;) !;PXP: -EUTE,#$   )hf% E@D,oYKJVi{u^G@Rzw< Gq{H #<Vjrm`PC7+  1kK /MdndK* *;@8' &($ -51" #7C@0 $! $Cb{y]8 !7BB5" %>RZYW\o[)Fhr^7*W~u\LLYjusbG*,;JWcmuxvoihswnwp`g?NI2 ~S0 2BH@/ (TT6MTI2 +Su{\5 3HK9Br_;  @KIpzfS#    2kh1Ai*J9G6XmjO) 3Wj`@$\Y4 CzoI* 0S|ZBIu9F#2"bAv|Cnq+1.  hZYe~Gd|@ cY|\8O3Cj$>C"Diyk_RC0 >hm7"9P]ZI/$09>>8. 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I X e n { (4AM[dp~&1>HUaoz ,7?MWes (4=GTeoz %6CNYamz*9K[iz -7EN]kv$,9GP`my '5DR`ku!,8EP[hs}   , 9 C Q \ g u    ) 6 @ K X e o  * 8 A O ] i x    ) 7 @ N ] h q }    % 1 ? I X e n { (4AM[dp~                  "                   "                                              "                   "                            ,KL+B_J1YM$R_W- OI0@]QI.E <(7=[SG?6U D;=[G3c-2\C"dQMcI;P[JCY- \4&KUR)?/*`>-R)$^]RB2P#>V&R ];H#4J!U7 @+I<a>T1(5A JB, 0?D2V-LS7TA  "b>@Q"#Y])TI<>E!R/c9MSX&@UWd;I6#E&a;<5 U"@U7L=UH'MD\JL=7;0DO;:W6:!//c\MU%>'HIEN D3`E45D^/9K)%LK3} OOE<jmcy<O&e 5[ze1{(J 1aZ'A/\eTqLOTB v!= (@,@ ?i\gUINE~ xC6@fdo,mlm 7Ecm/|i.P(=5=YT!\c04m!?Bzz3W4LJ5.q[$cbl/y!ZH5U$l, ^}cya H- `E@uPd^M$ZE7IoX)v'N"><7m~B? AG;x 1Y)  N. '   V    x%)|, 9)!<2LfpOf;,!Od+tl 9Q/,JU$9p-@Tz=j/I4' M>d_0<q:7TcH4sHLOI}o8i;zJYU"2GL+ a\1E_d @'w(u8nxG8t@R#|_Y^Y\jrdKz7D!&49XH<A9 XL C/?vGRIO"Re{u=W)x;eY(z{AlW*l=dM8bb)6_-;$sI=?/>XCX:Z7e?E /gtlO( W]>Zw>`%?t"/_LGP=P63+=H0&vC59>_$Z49L%OTCATAATTCCCACAATTATTATTGTTAAACGGCTCTTGGAATTTAAAGAGTTAAAGGATTATATCACCTACAGGGTTTGACCCTATTCACTGCGTTGCACGGCCAAATCTTGTTTGGATGGTTCCGGAGGGTGCCGAATAATACCCTATCCTATAGAATGCCGCCAGCGGTGTTATGGTGACTAGAGCTCCCCTTCATTCCTCGTCGGTACAATGTGGAGGTAAGAGCCCGGATCCGTTGGTGTAACGGGGACTGAGAAAGCCATCTATTTGCATAAAAATACCGAACGGCAGGCTAGATATCAAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 03:26:59 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTS_@BufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,>X|DATA ,>X|=?DATA ,>X|DATA ,>X|7DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~]LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISFP1AM3fRP1RL3fPP1WD3fURP2AM3fZP2BA33\P2RL3fWPBAS33H!PBAS33FPCON33OSPCON33N PDMFPDMFFPLOC3fKPLOC3fITPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%FSCAN3SMED SMLtSMPL SPACAVSPACFSPACAVSVER4.0SVER _5SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHFphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTS_@BufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,>X|DATA ,>X|=?DATA ,>X|DATA ,>X|7DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabmFVocmFWO_GATCFeat..m[GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~]LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISFP1AM3fRP1RL3fPP1WD3fURP2AM3fZP2BA33\P2RL3fWPBAS33H!PBAS33FPCON33OSPCON33N PDMFPDMFFPLOC3fKPLOC3fITPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%FSCAN3SMED SMLtSMPL SPACAVSPACFSPACAVSVER4.0SVER _5SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHFphDYbigphQLcphTRphTRITNn%&nAJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_hv"9;39*;(b84;U5)b3b=tr%3bbcy[*oCf!`LY&0AS_7eaPt;LOx,T2PYbWFU{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#9:%b?AM'3B[MLh7t+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4LHmXY#agsC!.:QI5&.XRz+*}Lq&Wi1oL]Wg*?u!&*z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPt}ASPt}AUDTdd{B1Pt|B1Pt|BCTS_@BufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,>X|DATA ,>X|=?DATA ,>X|DATA ,>X|7DCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabmFVocmFWO_GATCFeat..m[GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~]LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISFP1AM3fRP1RL3fPP1WD3fURP2AM3fZP2BA33\P2RL3fWPBAS33H!PBAS33FPCON33OSPCON33N PDMFPDMFFPLOC3fKPLOC3fITPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%FSCAN3SMED SMLtSMPL SPACAVSPACFSPACAVSVER4.0SVER _5SVER,,Scal@Scan3TUBEE4Tmpr<UserphARphCHFphDYbigphQLcphTRphTR