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A = / 4 : 0 $ 7 > 8 3 8 . & ; E 1 L E @ N B 1 5 9 E @ > A ) K 6 * > 0 5 0 5 + > K A @ X O G j [ K P M D 6 D E L 9 @ N 9 8 Q 9 2 0 @ 0 - : / A 7 , 5 9 E 3 9 + & B 4 < 4 , 9 = 8 5 ! ) 1 9 , 6 % 7 % ( $ 2 < 3 : 0 / : - 0 4 < E @ % + @ + . 5 ; 9 0 & E / / ) - - - 8 8 6 7 1 , " I * 6 X O \ e U ] Q ? @ F A 2 Q C ; I : : 6 1 > < D G L I F R X P P ; N L : B J / 9 2 < A 4 : : ? 9 < 7 4 , D 8 1 I H F 5 = 9 8 A - 8 3 4 , 6 7 & - 9 : 3 6 : 1 / 7 > / 6 ; . * C @ F 4 ' * : 1 @ 5 E F 2 O ; > Q K S 7 Z Y _ H . O > 7 U : @ \ Z ^ a Z \ D I A 3 = > R E 5 ; E 1 , @ 5 9 ; ? T 8 1 D ; K L A G 7 # < W 3 A B D @ H 7 @ B 7 9 3 I E B A : < : E A 9 3 1 7 ? 8 8 * 2 B K = A K E C 9 8 A Y G E Q Z F M G E 1 D C B U V L L E C R / J W G f V U f [ ` X T V @ Z U M Z T P [ ? K = N L F ; 8 B F 5 G H B M 9 F F @ < O H . : < ; 0 C H 1 C J = U O F J i \ b R L K < D : / : 8 < H 0 / ( 2 + = 4 4 3 @ 5 . D 4 7 3 0 ? 5 0 F > / 7 9 : , = * , 2 < 8 @ * 0 ; , C L 5 5 : ; 3 . 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` L = ; @ 7 8 ? 0 D = > E B > @ @ A C 5 : 5 C 8 M U S > T c F 9 7 I ; G A L X < 5 : = 2 @ . + 5 : 4 O 0 6 E 8 ; 8 9 D 9 0 8 @ K D : : N ? @ D , ; @ C Q N A Y N G Q _ A @ C J 8 K M a M g ? < D I C 0 ; 9 $ 1 - ; > @ ; J 6 ; P 9 E H @ G 7 > J < W F D D I ? B O ^ E M ? ? U C 0 B 5 B E L : @ 2 > 5 5 8 @ 2 / 2 > 1 ; & + 3 ? 4 5 7 # ) 3 7 7 8 @ * = 1 * ? . 6 3 < - 3 3 8 1 : , 2 < 6 4 C , * = 4 % + ' - # 9 ) & 1 0 . ( 4 % 4 + $ 7 E 8 4 + 2 ' A 5 3 2 * ( ' ? 8 I > ; / 2 , " 5 7 2 ( 9 ( F ) # 4 ? B / 8 7 < ? 9 ? 8 1 8 6 / : @ B % ) ( , , ) 8 B 8 " 8 B # = ) = , > 5 3 8 * ' , . - - " ' < : , $ 5 + ? 7 , E 2 5 6 7 + 9 D 9 = 9 8 < < / ? 0 ? E , < > < 7 ; G 2 # < 6 I N < = 2 G 4 A 8 9 O = & 4 . ! * 3 ? F < ; @ E N G ; < M E 7 , ) 7 = 3 7 7 3 0 8 - 1 + 2 3 A A N 7 < H L L C > B * I G G Z 0 - I I 6 0 / 5 1 ( 9 8 / ' = ; + $ , A 6 5 3 ' / ' 2 9 4 - 1 . , / , 4 - & - B ! 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bc5900697bc24f99b9f135cae816e4ceSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-24_01-56_0549 3130Seq 12249 12249_Costion_K1357_matK_R jmadera 96-Well
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_|$jQ,omT("T " F^ _ fNdaw[B2yr] E TNGGGAAATTAAGAATAAAAAATCCGCTTTGTTAAGAGGATCCTGGTTTTATACTTTAATTTTGGAGGATCTAGAGCGGGAGCTCCAGCTCCCAAAAGGGACTGCGCGACTTAAGCCTCTCTGGTCCTTGTTTGATAGTAGTCGAACTATTCCTTCACCAGGGGCCTGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-24 03:27:16 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt c ASPt c B1Pt b B1Pt b BCTS BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA # G2 DATA
# G2 + DATA # G2 s' DATA # G2 Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ Q LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS P1AM R ` P1RL R P1WD R P2AM R V P2BA
P2RL R PBAS o PBAS PCON e PCON PDMF PDMF PLOC R j PLOC R PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt c ASPt c B1Pt b B1Pt b BCTS BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA # G2 DATA
# G2 + DATA # G2 s' DATA # G2 Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4 FVoc # FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ Q LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS P1AM R ` P1RL R P1WD R P2AM R V P2BA
P2RL R PBAS o PBAS PCON e PCON PDMF PDMF PLOC R j PLOC R PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH phDY big phQL c phTR phTR Py]]~I8[e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|L:hmt$F!!IOP;T"CTU&qIlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,z-&l;u7Qlu19"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\f%\uDU00$Y+I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/A AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt c ASPt c AUDT d d ,G B1Pt b B1Pt b BCTS BufT & L L CTID CTNM ! CTOw < CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA # G2 DATA
# G2 + DATA # G2 s' DATA # G2 Y DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab 4 FVoc # FWO_ GATC Feat . . GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE , LAST ~ ~ Q LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS P1AM R ` P1RL R P1WD R P2AM R V P2BA
P2RL R PBAS o PBAS PCON e PCON PDMF PDMF PLOC R j PLOC R PSZE ` PTYP D PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 8
RUNT " RUNT / RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Scal @ Scan 3 TUBE C6 Tmpr < User phAR phCH phDY big phQL c phTR phTR