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N",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR?\cgggggefdb`_]\ZXUSPNKIFC@>;8641.,t```VVVzzz,,,888CCCONNZYYdddnnnxww                                                                             <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 525f7d78de2d4f979c2c0346f01ec510Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-24_01-56_05493130Seq1224912249_Costion_K1357_matK_Rjmadera96-Well K1357_C4_RKB_3730_POP7_BDTv3.mobSeqPop7z 2OkzxiR<+#$*19AK[o ;IJEAERf|zcC#%IpsU8 "   &,'" |{|~}sdSC:<HYgkbM4-9@CCBBBA@CKZl}|z$2u"R{h.i5 Bt 6s8#bhP}mT8" qhkuy`?0X uGlkHN|Y?1.2:?@;4.,/7CTkT5qQh'C|fVH8)"+352--5EYipk]M@=CPZ]UD-  $.588768<BHMOMH>3)%-Kg z wBHa6'Dh|vvz~|tfR;&-=HJC;68BNY_aabejmlf]ZdNo+wVD:4* )=KSSMD9-   $6L_kk]C#8*e- /}xfK-  1AJMNOSX\YN<&   '+) )^&O[GmG3,.3896/'!!,Gr=uR&6;4%  ->HG<+ !`3^|rFe>gbW%  !%()'# .u<4U/(CfU,       #    $*( +9AA:, './,&   I9FhQNTUM?45IkbF1$!',//-,.132/,-4?JMG7"   2|a)D>MO'_ p; /@OZbfgcZK9&  (13+ UP_sTqcT(sV $8MYWG/ $:^cbT( %:LW[ZVRMIC:.!#<ZtnP1 !'%    @j8: Z/81t[HH@8%9X!$a<^{zxdD$"i_]^r)f@1/2;I_zkL0  0@E?/    3BHD7'-LtoK+&:LZbc^R@)?~K '-4<CD?3"    9Q^]N7        &'" '176.# !(*)$    %=YvhI0  %*+' ,Nyzg\RE4  4J]hgYB'.88.       %;VthQ<+  "a^h&J$6o JeS|B)1OsoJ' 2MakkcXME>6-! 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W8[1'QLhc-Kmu.i(P1Tmx-/wO.u Y %t T!cKRT2MX_!8p{Tp! cZo PQ, &hK[>a8(f:>p]uEYXV)8F4wGz95E<:'JM]`"5$cbu*p(pG~J%M<VRXl(qpk+M{,PXM?Lt<>ckp2mnTdc2I3d}5 J:IHcOCp},DwUyrNs8/;]LgL,?)`"o8*TF\Y]8d_&,c}p]*HIg8K{U?E !)8jz dH12U,e@=%HV:5TpMU*:RKVouQD ng b3Bz<&x9p`rxjBl#WQ]z1jsb\e]-2P|5gg)N>1tYfQs6MlmXFocFpsQPqs(Up_W p\MW%/9+W ,'CI h1=?Vy+xx{|)UVS1pBbm bxu9j$&6>~7X1H)4hONqNKHWA R%Z1n MspO[>5qoO$;u]|044He:H@ x3:~QUC!ys*cRy#52_'Y ,:"5 z.#PN PgULu_kJ(~8B;A"XUJT+XD=!|ZE`q;[3CTTGGTACCCAAGGGGGGGGAGGTAGAATAGGCGCTATTATTAAGGGGGATAACTGCTCATACACGCACTTATTCAATGATCATATCGGTAAGAGGGGTAACCCGTCCAGTCCGGACTACTAAAAGGAGGTCCGGGGCGGGATCTTAACTCCCCCTAGACCGAGGGTCCAATAAAAGGATTAGTTGGGATGTTGGGGCACGCCTCCTCAGAGGTGGGTTCGCTAGTGAGGGGGCGTCCTGTGGATGGCGTCCCACCGGCGCGGGAAATAGTCGCCTTCGTTGTAGAGTAAACCCCTAGATGAAAGAGTGACCTTATTTATTTGGTAGATTGGTTCCTGCTGGTTGAGAGGGCACAGGTTGGAGATAGTGTCCGTCGACGACGAAGGTAGACATTCCCCTTGTAATGCCGAGAAAAGGGGTGCCCCGGAAACCCAGAGCAGGTCCGTTGGGCTTCGGTTTTAGGAAGGAGAGGCATCCTGAGCAACACAAGCAGAGCCTGGGATACATCTCACGAGCCCGAAAATACATGATGAAATACACCTCAAAGGTGATGGTCGAGCCTGGGACAGCCCAGAATTTAATCATCTTTTGGGGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-24 03:27:02 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtzASPtzB1PtyB1PtyBCTSuFBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,NXDATA ,NX=_DATA ,NXDATA ,NXDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISG;P1AMU^P1RLUZP1WDUcjP2AMUlP2BAUUqhP2RLUhPBASUUIPBASUUGnPCONUUWPCONUUUlPDMFPDMFGRPLOCUPPLOCULPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%G3SCAN3SMED SMLtSMPL SPACAV@SPACGKSPACAV@SVER4.0SVER u;SVER,,Scal@Scan3TUBEC4Tmpr<UserphARphCHGiphDYbigphQLcphTRphTR0qThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtzASPtzB1PtyB1PtyBCTSuFBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,NXDATA ,NX=_DATA ,NXDATA ,NXDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//aGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISG;P1AMU^P1RLUZP1WDUcjP2AMUlP2BAUUqhP2RLUhPBASUUIPBASUUGnPCONUUWPCONUUUlPDMFPDMFGRPLOCUPPLOCULPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%G3SCAN3SMED SMLtSMPL SPACAV@SPACGKSPACAV@SVER4.0SVER u;SVER,,Scal@Scan3TUBEC4Tmpr<UserphARphCHGiphDYbigphQLcphTRphTRqk+VxnDS\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|s:}@~B@68 zoH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'G,lwdRadJsV&=o?eU&GUkJZm{Z(c%Hhb+]A:LPZ`K$^KV>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtzASPtzAUDTddB1PtyB1PtyBCTSuFBufT&LLCTIDCTNM!CTOw<CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA ,NXDATA ,NX=_DATA ,NXDATA ,NXDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//aGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISG;P1AMU^P1RLUZP1WDUcjP2AMUlP2BAUUqhP2RLUhPBASUUIPBASUUGnPCONUUWPCONUUUlPDMFPDMFGRPLOCUPPLOCULPSZE`PTYPDPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 8 RUNT "RUNT /RUNT Rate RunN""S/N%G3SCAN3SMED SMLtSMPL SPACAV@SPACGKSPACAV@SVER4.0SVER u;SVER,,Scal@Scan3TUBEC4Tmpr<UserphARphCHGiphDYbigphQLcphTRphTR