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E E G : B * 4 2 G 2 3 ? > K V K U < 7 M J P L D U f \ > K E H H G G Y E H K A ; S A @ = P A 1 3 O / T 8 S 7 a : 1 8 : Q K 0 6 B : \ H N E c M P = / Z < @ Y P H ] J ] Q V E Z > . S @ D D 5 b T J + B W J L C / L T > - P 6 N X C W ` R I 9 _ C 7 ^ a : 5 S O @ 8 M 6 ? 2 I X K @ 0 G L B @ : N X 6 I 9 N K 8 S ; R : C B f F 7 6 ; B ; 0 - G B - ; 6 . F ; S C J B K P P 6 9 3 = L B ? < A d D < E d 3 B J \ L P S = I ? < N ? B 1 X U Q H . Z > U H ; N U W Q 4 2 G 1 E , D 9 = J @ K 2 : S C G : : N G E [ P N ` Y a F G A 5 I 2 > C Z V ' B A C D ? M J N : = N R 2 ? ] C Z E H : J < M = D A T H G E 4 G Q 9 = = B : 6 H I K / 6 [ 5 : D A J ; F I F : O F Q T < B V H Q 7 > T E F 3 V c b \ L \ A N X e R K < @ D N H \ K F @ P 9 G R Y J X 5 i [ 7 = L > Q 7 Q H E H S A G = S N D Y L V E = > G Q N R Y c P N b B G P D N C D P Z N H I [ B k = = Q A < ? D : J E [ P I D X L U E C W F H U N M R N + H T V F W @ @ R > I M I M J \ Z ] ? Q L I E < I F C A S L O = V N W 2 U H > Q Y O P ] M O : D Q S U F L ; i A C E X _ G S 3 V Q X o I [ V ` ] F d O F G R F N L P K 5 F > K C > = B 7 D 2 _ Z _ X R M T J U A = 6 F 8 = ; O W Y S G Y O G K P ; M @ C L T E < F H P D ^ O S e F @ M g R 8 S @ Q B L G 7 ; G @ @ _ X K Q K 7 % 3 B F = M L E N J X B M M L K U P C = K ; @ D N P ] O Z U [ F A V ^ M ; J 1 I L b < W R H U I < L D ] h Y \ Z N Y \ ^ I R S J J [ E T G W L J K ^ < b ] W H T < H F N H R N = [ P G H Y a W O c W J q T V O d e A < M E J R , ? ? ; W B J : L V F C I K ` @ 9 R D R N A N : 4 S Y ; H C A ? G 7 I N > @ Z O A B F Y I 2 9 M ] P M T B F W Z X Q @ G E M T 5 H Z K Y Z f M I K L 1 W A J Q ? ; \ U Z S Y F M 1 I > T e f Q H R ? D W R H Z X ! C M L = G ` W U F X P C < ] S < Q b R 8 a G Z M A J D W ] E @ P < _ m S ^ T U R F F T ? G 4 C P T H 9 I C ; ` Y V N V < P Z ^ Q G L Q @ N I G = @ F F P I V H M a Q @ Y Q G D [ V O Q B F ; L c : 5 W W U W A A B T M P P _ F G 8 [ R G [ M A N @ X K [ = J = Z E = @ @ N W f ` I S Q @ H N [ X U e G A R ^ X Q 0 H : O = E . F B K _ V : X k Z I : R N E < H P O X T T = H f @ X R Y Q j ^ \ g \ Q U g L N X H K W L P G e e Q P f B A E = H F 9 H O M @ H 0 5 : H B d \ H 8 ] H J X J U X K b O T ? : ] V D O O B X a F U \ j < Q \ A C [ D P C P G W I @ E U c N P W I Z F R a Q R Q H E O R R L K S 1 P S V K d @ F X W C d U f Q K B M 7 d ` G \ K ! J F Z S [ F g _ \ @ o X F 5 L S ^ _ J > ` [ X H ; V [ A h f R I ` ` K F Q ` P T I > F U S O M E \ m Y n R c = d B - N H Q K X [ Z H \ \ T X ` A Q Z Q S R C @ A C I D b J Z G g \ a c Z O V Z [ D ^ G \ f ^ f P a W I ` Q 2 > L @ U C O \ ] X 5 U R R L C S O U V c Q ; [ f U W g d ? ] _ l c M X V D F O L \ d V i Q L Y ] \ Q U 8 V \ Y G S : , = @ E G ; [ W a T ^ X P R U M _ R Y e W d _ O Q @ W W \ l H X U J / a ] R a V Z > E D L g b O H U > X I D L k W > M S d \ I d @ U J M G a Q O I T [ L = g G ? Q : T O T 0 S J e 7 B J P I f @ X I A B E [ e L c Z [ V ] Y ] ^ t d J k a ] : F Y J T F \ B N J @ S O U [ ` X V W p S f ] ` W i M f @ P Z R U K ` f _ H t Q Q Q U \ j Q ` d ] E T O ) F [ J n P N I [ K R ; ~ U [ S K J I X P R X [ ] ^ @ Z V W P G X e ] f l S J b T c d a Y g a n W X c T ^ _ ` \ R T j f ` D I T O b Q K G S j ] ] i J [ c T ` W N S D H 7 c T D N Z i L N H m W ] K h P N X Q ^ V ; M b R N { h N ] ; R [ [ e W P 5 O c M _ I = I T o T i c D b M g I U O j l L ] N N ] S f A R I M N j X X Q C a Q Q N @ Q C Y F V O m P M J G O ` R L J a K i i ] ] d g j R D W d ] \ l G O O M F I c _ ; _ Q B V 8 K P ` X N [ B b < C Y _ 5 K W [ c N K P Z K a Y Y O G a M T G ` Q U @ E G A [ ^ R R I T K \ P T l K 7 6 T Q W X J ] . b I ^ P ` P i O K T J V d n X M O M F Z e T u K _ X [ S m Z D P s K [ \ ; Y S V S W L T R d Z U R K ? [ P \ ] L W k f S D ? i W @ U _ V g I X ] _ o n r Z \ G U W ` _ b a L ^ A ^ V B E Q P a 8 W K g T Y W P F O T Y a [ _ e E > J Q _ = ; [ i T Y M V ; C 6 ] Q N S d < Q S b d W Y [ Q X W ^ S ` ` t P Y l f x l G O N R k e r T R C G Y K X Y l h G Z L I > U [ C 8 G \ R G V R Z ] V c W H > Q X J E r Q T S G F ` l G C > M @ R K 4 O H l J X Q f o c N J S G G ` _ W O j N : I [ I M J P e ` C [ _ N K X O \ R C K ? 5 b D R T P p a 7 k V { z Y S < G ` G h N d ` [ M V T Q M P i R F T j J m N V k c g J U k ] q a e \ S g S S Z Y ? c G K h b 6 U F ] Q : ] l a K F Y [ i ] ] h g Z t _ ] V d R i \ S J P N V d _ k U ` f = O ? K R I ] V o R [ J \ X U M Z q { n Y _ ^ y m i ] S T k b W [ Y Q W c d L W b Z X [ D \ M P S ` a S ^ Y C a [ ` o [ l ^ O h b T i O H H b c i _ X f W Y : m _ D O R h I Y ` V O J Z B S ? e M U Y e = Q ^ Q P Z L F e G i d T ` I V ` Q ` j Z ^ J b U b c J J F K W a U t g Z _ b e d ] M ] N M r e V b S Z < M 7 U f p E M _ ` e D R P [ K ? g a P k C T H d Y F [ V T f T ^ c ^ u ` e } _ G f ^ Y | Z Z T < Y S y y _ m i J L b N V a P Y R j K n c X K e x } i t e d c B _ p K b \ g Q T Q [ n g e [ d c \ V i g ^ k \ [ O V r N [ h U = _ Q _ n p _ j H t O m \ ` [ b \ V S g c m [ ^ ` N T n ` j h ^ d g W P P h [ E S ] ] n h H 8 j ` h W L L a R ` F ] Z Q q i p c j W d _ j T R Z K p e ^ e n U W d g ^ R V M Z H e f O ] _ \ O Z V k a d f ` f < T B W Q T b Y ` h ` f e N h i [ e _ c U ^ T Z h d p ] o g _ g r T } Y c [ R e ] [ ] > _ Z h K K f g Z y h N i ] Q l L _ c P S Y P j ^ O f W W I P ^ j ` n V 5 Y T a W H o k K P e l K c \ y m k _ \ X Q { [ \ R x ` n m g x o r T I g { S Y c ^ u ^ s S ` Y L e U b ] z b Z Y V V z w a o [ Q X c t T f M i U Q [ g p U [ o O h h ] h V d b x p j ` U Y a g X ^ A T f [ N R Z m k Q e [ Z r ] O y c M @ T o C H e b q f V a i n c Z [ c g Y W l n V j i Y \ t b b S h Q g \ l ^ i g [ ^ c ` [ ` V V [ r q z M j c _ D E C i l g b t b _ Y [ W Q i e Y h q w T ] l l m Y L V V O O S t f q T Q ` ? ` j d T t S n j [ e G D X Q R I h [ K d l d X S e ^ U > C c p m A s l Q y X Z f o \ ] X b \ o y a P e P Z L e k Y X l \ f U R E d ` w H X _ P B g O O X g \ Y q a N P a h ] Q ] ^ g n f j N w Y Y ] ? R n p e p n J a _ g _ Z ^ _ @ c = \ H f e k h P i p V V \ n V U W c P u _ h r \ R P U C g W Y e i g X F N Y \ U a _ ~ f x [ [ l u l u J T g W Z _ d \ _ g a T b _ ` ~ ` _ S U M ^ i c q d h _ Y p e X v f c r e S U Z } q _ N ] ] _ a e J Z H W ^ u Z z l Z P > [ X A L b M \ f T Z _ o \ X d a o d h P W U i w f u q K O c g Y D l _ ` t g = F d Z g f e X P X k O [ c k ` x _ p ` [ f e g l S n j Z ^ R r f Q r g l p c [ ] ` m i Y \ c ~ i r _ N ^ o W l S u c \ e a V X T f h W i b U } i p m _ o i b d r g c ^ V s j p Z i c k p u Z Z j ] t r x m ` d N [ p n _ _ k b _ S g i X W f p N g l r g [ f l \ g j d j e k g P Y y i ] R k p r q b \ g l d p f o t z m t w ] j Q e m t j k p t e j v y ] z k R d s Z P g j r k ^ Y Q ` v f l w e k m l j ] [ v e e \ f \ k c X k W G ~ f h | T n i m v ` _ e Y [ ^ p y c W r u } i p s p c y h r b n ] m k Y r k m n m W n v ~ i n m h p [ g _ q q f b T c b T j w m { p s l a i u r r c d o l r a o V h n k g p w d m p T v s z r U ` h a j m k e v | h e ` q f u { X z e w i v y d p p m h k m r u t ] Y I j d Y S W } N t y a I q A P j ` H x g k Z ` ] Z ` i \ ` a i g | E a k V p b i W M l a w j b h { r v n V h h u p d q | w ` j T g p W U ` z p | [ v W a T _ r X f W l q i j ` f a d m i i h f h ] n f _ _ q U | g y ^ m g j ^ ] b ` d c n j Q \ t M c t ^ s r ` Y ^ h b w \ ^ \ _ l a h Z p Y i l P P l O b o i _ o N m \ ] q ^ l X h j T [ i ] d h ] p ~ q o B i p T i O ` k U p { g [ j p ` j c E n ^ j S b W _ o ` p [ z ` h c h x W a f s j l d g Z a q S K f ] V v L c H b C } N R u s _ T a [ i K x s o x p w t ] y e h c n ~ W ` d k h h Y | r
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8d7690b5a7c44363bd149d9a5f3f6771Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_F7KB_3730_POP7_BDTv3.mobSeqPop7z5k</P-Z\
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DABZ ASKB.bcpKB_3730_POP7_BDTv3.mobtermAGCGAAGAGAGCGCACCTATGTATCTTGTATGAGCTCGTGCCCACTTGTTTTACTTCTCTCTTCTCTCTTTGAAATTCAAACGTCCTTAATCTAATCATAAGGAGATTTTGACTCTACTTCCACTTTCTTGTCCATACTCATACTTCACTTTCTTGTGAACAAGCGAAGAGAGCGCACCTATGTATCTTGTATGAGCTCGTGCCCACTTGTTTTACTTCTCTCTTCTCTCTTTGAAATTCAAACGTCCTTAATCTAATCATAAGGAGATTTTGACTCTACTTCCACTTTCTTGTCCATACTCATACTTCACTTTCTTGTGAACA 9 D \ j ,4DN]mx".6?LXamx )1?JYgp} )5HT_jv "/:FMXdo{%0:FP\frz &1;GS\gs} 9 D \ j ,4DN]mx".6?LXamx )1?JYgp} )5HT_jv "/:FMXdo{%0:FP\frz &1;GS\gs}
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'VpAi 1; f(>c V 0 S [ ;> Z TTTTTTTTCTTAATGAAACACCTCTCATATAAGCTCTACTGATTGCCCGCCGTGAAGATCAGCACTCTAAAACGTACACCCTACATAACTATATTATCATTTTGCTAGCACTTCAACAACAATAACCTCCAGAACATACGTGTTACGCACCATCAAAACCTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-20 10:56:41 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt | ASPt | B1Pt { B1Pt { BCTS g BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 5 LAST ~ ~ LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM D , P1RL D
P1WD D p P2AM D P2BA < P2RL D PBAS z PBAS PCON
F PCON PDMF ] PDMF PLOC D ` PLOC D
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER \ SVER , , Ǿ Scal @ Scan 3 TUBE F7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt | ASPt | B1Pt { B1Pt { BCTS g BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3 DATA # G z DATA # G DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab $ FVoc $ FWO_ GATC Feat . . $ GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 5 LAST ~ ~ LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM D , P1RL D
P1WD D p P2AM D P2BA < P2RL D PBAS z PBAS PCON
F PCON PDMF ] PDMF PLOC D ` PLOC D
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER \ SVER , , Ǿ Scal @ Scan 3 TUBE F7 Tmpr < User phAR phCH phDY big phQL c phTR phTR XPvc.[.Iu2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*27CP%23$fuuo>`:9HFfUmbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E~-w%g,4c~Q9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23i8Fb$tMz+6b=J#=p\E (`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVoJ@G~ES[56? oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1