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  ]]]SSSvvv   ###///::9EEEPOOZZYdccmmmwvv              !!!"""####$$$%%%&&&&''''((())))****++++,,,,----....////0000111112222333334444455555666667777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d05bc9c9fce14f67ab6ce27dbe68034aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-20_09-08_05303130Seq1224812248_Costion_K1357_matK_Fjmadera96-WellK1357_E7KB_3730_POP7_BDTv3.mobSeqPop7z!,//29FVi{>a/^:UcbR;$   qaR>!zg]\blv~}xtrsvyyul_RF<5.'!$& gXU^m},AOX^bdc^TH?=FWl~8Q[ZTRXi #=[V$gtt5}"|D H{ F:Ax}X&2Q1umkoy3jl?hTG>952/,("!&/>So,6;7- r\QPU]bcaaen{wj_UL@3$    '>\ 8c_W+ #'% ,C_~nchT$*WKrg7Y9& ""   +Dc Oxs<l7   7OanwrS6   +C^ywcO;*&>YqkN0 &;X|eD+ .]?VT: Z+ +NsxplibVD.Z4;3?t3 +7=<6-# +6?B?6*       3\}O*/Ore?  #/9?@=6-" #! 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""                                !$!    #)+(     "                                           %'%                         !                                                                       %*+&                                                               ##  #                                    PT@AOAPA KB.bcpKB_3730_POP7_BDTv3.mobtermCCCCCATCAACAGTCGTGTATGTAGAACCTACTGCCCTGTATTGACACTAACACGTTTCTTGTACTTCTCTCGGATCTCTGTGCTAAACAGTCAATCCTTAGTCTAATCAATGCTGGGATTCACATCCATACTTGAAATTTCTTGTCAAAACAAAATTTAATATGGGAAATTTACTCAATTTCTTCCAGTGGAAACAGACTGAACGGAGAAATATTGATCAATTACTTCCAAATTTGTACCCTTACGAAATCCCTTCAATGTCTTCTAATGTATGGACTCAAAGATATAAATTTCAGGTAAAAAATTATGCTTCTGTATATATTTTCTAGATTACTAGAAAAAGATTTTGTCTTTCCAAATCTAATTCTCAATTACATGGTATGCATGTCAGCCAGTGCCTGATCCGAAGCTATTGCATTCACTTCGGAAGATAAAAAAATAAATCTTTTATTATTTTTCAAGGAACTCACTATTTTGTAACAAGGTGGATTCATTAAATTTTGATTCTTCTCATACCCCCATCAACAGTCGTGTATGTAGAACCTACTGCCCTGTATTGACACTAACACGTTTCTTGTACTTCTCTCGGATCTCTGTGCTAAACAGTCAATCCTTAGTCTAATCAATGCTGGGATTCACATCCATACTTGAAATTTCTTGTCAAAACAAAATTTAATATGGGAAATTTACTCAATTTCTTCCAGTGGAAACAGACTGAACGGAGAAATATTGATCAATTACTTCCAAATTTGTACCCTTACGAAATCCCTTCAATGTCTTCTAATGTATGGACTCAAAGATATAAATTTCAGGTAAAAAATTATGCTTCTGTATATATTTTCTAGATTACTAGAAAAAGATTTTGTCTTTCCAAATCTAATTCTCAATTACATGGTATGCATGTCAGCCAGTGCCTGATCCGAAGCTATTGCATTCACTTCGGAAGATAAAAAAATAAATCTTTTATTATTTTTCAAGGAACTCACTATTTTGTAACAAGGTGGATTCATTAAATTTTGATTCTTCTCATA)5CLZfs$3AM^gu #-9BNWbo|+8HS_js%1<FN]it(3:GQ\lz !,7ERany )6=IWdo|$0:FR`nv  - 7 C N \ e n }   % 3 > I V ` l w    * 4 @ K V b q {   / 9 H R [ k t |    ' 4 @ I X c q z  !/8GQagu  (7FOZdr~(1=JXcq{ &1?JWclx %/=IS\nz *4=LYfq|)4@OVft}+4BKXao| !,8DR^lw &2AKWdox!-6DU_)5CLZfs$3AM^gu #-9BNWbo|+8HS_js%1<FN]it(3:GQ\lz !,7ERany )6=IWdo|$0:FR`nv  - 7 C N \ e n }   % 3 > I V ` l w    * 4 @ K V b q {   / 9 H R [ k t |    ' 4 @ I X c q z  !/8GQagu  (7FOZdr~(1=JXcq{ &1?JWclx %/=IS\nz *4=LYfq|)4@OVft}+4BKXao| !,8DR^lw &2AKWdox!-6DU_  %        * '          ""  "     """        " !"                    "    !"  !    - "  !" !     !!+      "  "$""   "+       %        * '          ""  "     """        " !"                    "    !"  !    - "  !" !     !!+      "  "$""   "+     50 7R[8a$6SF[Y[;LSCX.;%SV7* `OF?` : =,=)&(-9WHUJ=2*BE? PZ4OW,5?7@32c,6#Y]@$L ?^$KFG^P`L=;%&^"D KGQZ)QW.GS-NK<9N)%R1 *HJZ"(I_\R87J?BYQO;7A>7R=: M2^ca12 ab,cXb[#8=O'7>5Xc`R&?@J:J=[G7L`1(%.^>F/$,C%QV T!&F6@8%54\`56%5]XT' ][X"#/ N XTAcMIJK\ Q-R[)9%(? 2 11a"YQ-OY0A >:9UD-"+NPQPba[/6C%0/V(%5=\ 1^ MQ#99< ^1U)bE)10MM4 & F , %VDENI46aUYc \(LW'`%<(6YX!b2V"_64:=I]^U!=^@A&abNG*c1bW ]2'M;o4tFPRs( [ p'lKh{iecc"y) 9qss   ^c2+cIJ f; ~uc n`?9 nn{"8rP Je hvr+w"}P|A!?; % P71';~f 1@mOB"Q\``|TbO~1DD{pHvfCTf8VPIqr7fh*YW}j#uGs/ B$hIeaZ:~O;Y4#PCn3!zP}V;-g(0^oEo om AW{ -mf^"C7_ Z^>$W+yg;ot=_Bx HKAR H "F7ML]XyP* MBdBCM8Vb?$?QCTNn4WbSLGZ,*X4 c 0. +&PM';-%(&s "3^+$"3>H*)  [-,<[-KO+* M"-3G_*T?"u2`6'-V,Z2 Yp&.X+&ynO"Vbhv {,1;p^a} ynN )(/sc RYu$\//*d,RoI{05e~z,U;`7dz8p~=}+%au;T#^wm%y*yh8tomCd!?M 2 LO| Tkz^@~-E1$/LLL">J98IWDp&9RvraP={X8#m <Wm$p1mfjW9ih*ME 5'J5nd@ @Z&F#UJI[l](+II./xT.!.(&Y6z|9Au.|!82 EDd[ z r7) \ ?!\7s7,*5<=ertZq %DKb[@wNsy4zRdr'kh5B6DJH&bRg|8?t8v>v5PXKuh!ul6RH) @F8!]rUkY-I0^QVBJv-<pY8<8q-YqUW:Mn^ [}A;_(J&{ P%(G'[P&Nqs'VC5+Yg$#;+m=PiL#>iPn]"f z5xmWSAe]0W[.   |B=]^jeY7R%3U+YAj6DLs\{Y:GAAATTATTTTTTCTTGTATACATACCGGCCAGCTAGGAAGAGCTATGACCGTTCGCAGGCAATAACAATCTTCGAACTAAGTTCGTTTTACTTCGTTGATAAAGCGAAGCCAACCATTCCTTACAATGACAAGCGTCGGATGCCGACTCGGTTCTGGACGACATTACTTCAAGGCTCCAAATGGATCTGATCCCATTTTCCTCAAAGCGTCAGACTGAATGCGTAACTATCTCACTCCTATCGGGAGCCATGACAAGGGTGGCGTATTGTATATCGGATTCTATCCCTTGAAGTCAAGTTGGTTCACACTGCGACCTGCGTCAAAAATTTACTACTTTTTTTACGGAGTAGCCCTGGGTATAGGAAAAGCCCATACCCCCGCCTCAAATCCAACAAAAGGACGTTCGGAAACAAAACCAACGAGACTCTATATTCTCTCGGCGATCAAATCATCGAAGTGCACGGACATAACAGGGCCTGAGTTCGTACCAACAGGTTGCGCTGAGAACGGACCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-20 10:56:43 -04:00AEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPtASPtB1PtB1PtBCTSBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA 0a}DATA 0aNDATA 0aDATA 0acDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOISs P1AMhP1RL`P1WDpP2AMP2BAP2RLxPBASuDPBASs@PCON\PCONXPDMF]PDMFs$PLOC{PPLOCwHPSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%sSCAN3SMED SMLtSMPL TSPACAR*SPACsSPACAR*SVER4.0SVER  SVER,,ǾScal@Scan3TUBEE7Tmpr<UserphARphCHs;phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPtASPtB1PtB1PtBCTSBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA 0a}DATA 0aNDATA 0aDATA 0acDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTaboFVoc^FWO_GATCFeat..0GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOISs P1AMhP1RL`P1WDpP2AMP2BAP2RLxPBASuDPBASs@PCON\PCONXPDMF]PDMFs$PLOC{PPLOCwHPSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%sSCAN3SMED SMLtSMPL TSPACAR*SPACsSPACAR*SVER4.0SVER  SVER,,ǾScal@Scan3TUBEE7Tmpr<UserphARphCHs;phDYbigphQLcphTRphTRZsbJEP'Z]FO{7I{6Emc9_lN#j`KJX?-'?C,F4te?t;t*2]>57_X~GjW<72s-@M+9mW'>YP~jM.9Wc@>(Jt6%> :L-Ev!$V@AK1ql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W?p<;RAX(pAjw[~W@0$.9@_05a=gHFhwD~ ')&o*5V3"WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$b!;|dxU[YCC/3[`kI ?"U27`N`3[)|$X[@5+'pm\P$x`8c_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl69ep>.]#9!yVHRV9TkE8ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~,AX@_F^L^E3Sk-[qr[&X1EDE?]mnjH[$.}RZ#3|5O-U[d1DFW%j'kT'_C:Or4^7~s?K90lVSFsA bXX<,x'EEwiOfFWcDArh]|Ko5qS+SP7T]Tcr[&$&?_1OPh~jTn/])RpikE:b&b)>`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN tAPXV2APrN ȧAPrV1APrX}}ȰARTNASPFASPtASPtAUDT``B1PtB1PtBCTSBufT |CTIDKCTNMQCTOwlCTTL tCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 6DATA DATA DATA 0a}DATA 0aNDATA 0aDATA 0acDCHTDSamDySN UDye#DyeN`DyeNeDyeNjDyeNoDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTaboFVoc^FWO_GATCFeat..0GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~LIMS!!}LNTD2LsrPaMCHNMODF ȞMODL3730NAVGNLNE`NOISs P1AMhP1RL`P1WDpP2AMP2BAP2RLxPBASuDPBASs@PCON\PCONXPDMF]PDMFs$PLOC{PPLOCwHPSZE`PTYPtPXLBRGNmCRMXV1RMdN -RMdV1RMdX6RPrNRPrV1RUND RUND RUND RUND RUNT  'RUNT  7+RUNT  . RUNT  5 Rate (RunN""!S/N%sSCAN3SMED SMLtSMPL TSPACAR*SPACsSPACAR*SVER4.0SVER  SVER,,ǾScal@Scan3TUBEE7Tmpr<UserphARphCHs;phDYbigphQLcphTRphTR