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X c h X y W c ^ S U L M R 9 F ` T S M Z ; 5 : ( C + K A P A L B 1 P Y E < I D : 4 7 6 6 ) C < P > L = H R # < J 6 1 7 / 6 ) ? 5 9 C 7 3 " . 2 # / 6 " ) < < 2 ( G C S L Z 1 3 @ ^ S G A 6 ; V X \ R n b G O W [ Q E @ C 6 + 7 6 F 1 / + F 2 @ . / + : ( > 1 2 8 3 + . 4 ; - , 8 F C 4 8 , D C A 6 " , , ! 5 9 , = / B 4 , ) A - E G ; = A D T ] K P H N O C , @ D D ] G M K P T G : D A 3 0 > b U E F M A F > ? 4 " 6 C 8 , * 3 3 > / K J D 0 ? C 3 D @ c , M K C T D O T _ l I V N R F R D L ? H 4 / G 6 H 6 2 c Y N A Z 3 I G ; S F E 9 U W 0 ? C T G g O = ; H D 6 I G X W H H c T W J D # 2 B U / M ; E C G ? N U U L 3 M Z L D O ^ f E U L 9 ; @ = I Z : P U N X ] a Y O < + J = 5 8 S A V R G [ V K ? U U [ _ < e O [ S k ` d @ G Y V G : 9 F 4 + 0 0 = 8 - U K % J 2 C ' , 1 E G E , B O J V @ J 4 C E B = 4 5 5 6 B E 3 @ < @ b ? @ # - 0 K 3 < 1 M ) 6 = D 8 C 9 8 P B H K 5 G B A 4 > K : E S I 2 4 3 ) B M = Y , > 8 : 7 O 1 H 7 1 5 O M ? 6 A S T S K , Q R G _ ? D @ 9 @ 4 H Q @ L Z a _ h v M ` O Q P ? G = P M Z P B R O @ U P P \ R c U G S A C D : E O 8 6 D 2 K O V M R > H I D [ J I 8 A N G = 8 K D @ R ` O K 6 8 0 > < 5 K > V [ N R c g _ a f W U k ` x \ d ] a j A i f Z L G / Y S Y G ` h : j c m ^ l u o S d m R b b _ j > M V T N W B ` Q k R W N T ? G D ^ Q 6 ; O G 0 7 K J 0 G = N = # J 8 D B < P H ^ Z c Z g L l ] X l i d j d f w i \ v j X b \ \ k Z m m f ~ p z ^ n Z [ R 3 L \ 7 D Y G H J T ^ S F Q W K C _ \ Y R M S ? Y S K 8 D L > c K ( T J I A 7 7 6 V H @ 9 6 6 7 ? 2 > ; 7 = H B 1 C N : ^ X B o a Z P g P T H H O Q \ Z f o o [ ^ U ] J V [ a ? d d ] F K V W e h U V b Z O J A W h Z L [ ; : H A d R ` D = O I i 5 7 M G 0 ? 3 7 D F d J O J E E N M I > = ^ A G O G [ R L k [ G ` A Q A 9 5 F a U V S V F a [ _ _ L \ g _ H Y V : > > N T O N a Z | [ h p U \ g c \ P k q K k C C k n P ` H R I S Y d Q d [ a L { v \ Z d b O _ W m d l N V d [ U k a N F O P W L F P L ' 4 ] Z Y E 7 S [ Q @ O \ ^ b ^ R [ V g v ] a Z t g r Z O \ U Y G @ f K C J J r _ [ ? Q P Z _ ] i ] c \ I [ R E L J Y F E > + A = 5 7 : = ; - 1 F = 9 C 3 8 < E D D 3 F [ : * K I O 7 P I F E k J 7 A ? Q H G G A I Y + S G x c I 8 \ c S Q T H j c N \ Z 9 Q G S + I G I + H @ 5 4 O 5 W J a T _ H ; : 9 @ 9 ) 3 H ( % 2 1 O ) 1 7 : ? : ] G 8 F 8 I E % < A P O E ; < ^ h [ E 8 U l I S B ; M > H A E C F K B < > 1 d E G E N = O N F [ E * : ? @ U + 6 + B 6 3 B 9 U 8 # ' C B 6 / 6 D # J < H 8 = . / 5 1 9 @ > & C 8 Q : G A + , ( 4 3 R V 8 9 , : = M " @ @ T = C . N , M / 4 D * < = @ ? J T e ( & / J 0 5 3 9 P g N Y 3 I R P ; R N T C Q b : B Q V H G N K ` 9 M P B 4 ? V D M N : ; L @ 1 G > G 4 A $ E ? N : < 1 F L ` ^ I 9 @ 4 B ? D , . 8 . P B ( J A A ; G 1 F 9 > H D j X W F 5 G O 9 S E Y P ^ = C M R N L P K L H C M < 5 R L 8 & D C O S X 3 o R f / G B 3 W C 1 = 7 T 2 3 C R _ Q L 9 G : P 2 K : G J O F 4 \ ? H F N & R Y M K C J L 9 @ H X W Q ` T @ E [ @ D Z H D Z G 9 a M A ( - f ] ^ d e > Y C P S 5 F ^ C < : @ : 1 , 2 C ? @ E K C = 1 < G I H I N N ? = K = > = : > @ 6 . C 0 U $ G M B M F : A @ > E = 2 6 P K E ? Y \ R O D R Z Q Y F M P [ U j m W ] q f Y Y q b k g p Y U S a L E V g A X P K U ? X L e \ ] ` @ V X 8 P S u R V N M X O ? O F g \ T g ^ T l O n e f a _ w n e e ^ U S U P T Y K h N @ F D Z S X X < H M [ f Z k R W O ] _ P j T K ] \ N G < Y M J K _ N h Z Q V _ ` f [ h o U p W b q b \ V b X [ ^ j _ W o ~ a _ J @ O ^ ` F M v n ` b _ f ^ _ g W U H U / 9 = O \ _ A e ^ m F _ n Q ] [ j Y c f U j c a M h a X ` l I S v u d j p ] x p q b t X l \ _ a N [ U M E g h Q L V M R V K K T g ` K N A \ O F ] Y * M B [ D H \ X ^ X ? \ ` g H i [ X [ c Y h Z \ N c b l X k p m S j S M T e h D y Q p b s t f } n X S l q ] S d j U g L _ K I L I T S T f J C Y O 5 G b q M ` A e ? p c M F 4 P 3 Q 6 B M M ) 9 $ @ G ? G - - ; K I ; B J @ S V L H L V T [ P R X \ j P h P H F h b P ^ > ` G g ^ t p f _ f S X F L N B Y U L > = @ S H L 8 O G P E @ < H k R N N h a [ ^ i E X C N 9 _ \ O I O + H W 6 : ^ K T a d a ` H b d f G f H j _ J U ] _ _ e V n L 3 S Y = ^ H \ h S \ G P B B G C K @ + 4 E b S 1 Q T 3 A H ; = H 7 Q R S D J 3 2 E J F F 5 W L O c G 0 C N f 2 B M A 6 J V C 7 X h B C _ R Q ] Z L U B F 9 N E L , X K m Y f ^ z c ] Y X ? D h W R c L D 9 8 E J E B J ] $ 9 E @ D 7 > @ ; G < D B A K N D 8 ; J : . > 8 G < 0 E A 6 > K N L N D Q ) N O P C + , < L A J U N N I L P Q V B U l ] Y ^ Y T N M ^ j T ^ Q H ^ Y 4 A T ] Z 8 I I [ R 3 6 5 , X ; 9 W D [ ; D f @ J T Q G A H = < : d N _ I Y [ J R : O [ @ F J Q G < < E C X S S N 1 U I @ B L M F @ M = ] A < R P J O c _ F O J \ T M B c b W t 3 Y P = M M N W N 5 H J O Y > L < N K A Q X D Z b V J P O Q D @ P 9 B J 5 9 X M a [ ? O U V m t R ^ n H 6 7 A F > S : Y P @ O L Q ] H 9 \ 8 L A I Z S E D L P O Q e J p V J e l d N d H N F 6 0 H S A S U X 5 7 [ ; R B B / _ \ @ C ? V Y a 3 K M 7 h W M H 6 = > C S F Q ` Z K [ J F ? Y Q c N A \ L G B J t Y ] I ` K b W D L _ _ Y T V d \ c O t s ] Y d Z T O X a a l S S 8 _ [ U X I Y X [ F W > N E ? < S K K R J O R N G L \ > J J 3 [ R J J ? i V N R X _ E H X \ ] i g ^ j ^ [ J N ? 9 B 8 \ > K F L ] @ Z R Z T L N 9 V E ] Z N l @ s c i C ` Z m Z f W _ d T R ` J M H ] a N ; Q S C L \ V F 7 > C ? i @ _ I ; Q C & g [ N K X b J L J j s f S K [ l Z a 8 O ^ f \ Z N R a M F a T P M Q b T n Z i V ] o J a Z I ^ j f e _ k j b e g i I I d q X O T B d S o H Q G d B E : G G a T [ r q V E Y ` N ` [ U X | m a Y U c j _ Y k W i p e S Z L r j J V m R Y X L m ] 3 ` Q e c ^ Q g g U O A W W U A Q m c ` X Q N o 8 p U ` T k b 8 I d U Q O a K O = Q T H F T H D D Q R g N b ^ d c T U f \ b N ^ a d Y T B Y c _ K S J b A Z 8 T Y > Y R O . R g L W X f K T @ ] J F _ R O E a ^ U X Y b f i I S S G R S M M E V g O R @ X f D l S T Q T P i S L R V C Y X T W O = a V V L P f O [ Q N G ? = 4 A Q M I [ D L I F i [ k P j s R 7 Q F ^ L ^ c H O J u ^ F T c Z X I M a Q W M ] N H [ O c W K ` Q \ Z O h 5 J j N R b f r c ^ v y f t s \ j c g p I b U d Y S L Q D W a _ V : Z a X e T W [ R X \ Q T P Z ^ ] R d O L F I J U j X V V T ` @ ^ X P r d e ` c K Z W a m o E Q O d z d ] [ f D F [ \ V L f b [ = G W ] v l T _ R _ e j ` M R n M X g R O j d P b D R a k a m a n f ` c j U N n Y U m G N j M K 7 U K P C S I 7 e T d M f a R [ l k ? R l D g ` X O j T b i f e u v i b U w p T q O _ ` K h ^ L _ ^ I h U R W ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8d79492128f94b138dfca4243317bb2dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-20_09-08_0530 3130Seq 12248 12248_Costion_K1357_matK_F jmadera 96-Well K1357_A6KB_3730_POP7_BDTv3.mobSeqPop7z 2U{ ?v)PnM'u`{ &
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Z8yoQA \C1Qaxw Qd @Wz +p!HY/9 .Eh? ?hFq]4Z- W &W*|, n rM|{T !\BpyolzJi$)pNX>O10K\4 ~/UNxCATTATCCTGAAGAAATCTTAAAANCCGGGTTGGAGAATCCTACCAGGCATTATTCCAAAATACCCTTGATCGCTCCTCAGTTCGAATTCTAAACTCTTCCTCCTGAAAAAAGGTCTCTCCCATATAAATATAGAAAAATATACTGTAGAACCAGAACTATTATCTCGTTGGTCAAAAAGGGCTTTACCACCCTTTTGTAGAAATCCTTACAACCTGCCCCATATAAGGCGGCCCGAAATATCGTTCACTCTAGAACTGATAGACTGCTTTATAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-20 10:56:37 -04:00AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3} DATA # G z} DATA # G } DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ ! LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM & v P1RL & P P1WD & P2AM & P2BA P2RL & PBAS PBAS PCON = PCON * PDMF ] PDMF PLOC &
PLOC &
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% } SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ǿ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3} DATA # G z} DATA # G } DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab - FVoc , FWO_ GATC Feat . . , GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ ! LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM & v P1RL & P P1WD & P2AM & P2BA P2RL & PBAS PBAS PCON = PCON * PDMF ] PDMF PLOC &
PLOC &
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% } SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ǿ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR 4{,|Qv%F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBI.]#9!yVHR(qTl4Qml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrGMwQU7Bcn>N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/37h3C(&Nil2vM3~:,;k(921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Ut.KBz *#$O+I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/A AEPt 3 AEPt 3 APFN t APXV 2 APrN ȧ APrV 1 APrX } } Ȱ ARTN ASPF ASPt ASPt AUDT ` ` ; B1Pt B1Pt BCTS BufT | CTID K CTNM Q CTOw l CTTL t CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 6 DATA DATA DATA # G } DATA
# G 3} DATA # G z} DATA # G } DCHT DSam DySN U Dye# DyeN ` DyeN e DyeN j DyeN o DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab - FVoc , FWO_ GATC Feat . . , GTyp ! ! 4 HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 0 LAST ~ ~ ! LIMS ! ! } LNTD 2 LsrP a MCHN MODF Ȟ MODL 3730 NAVG NLNE ` NOIS P1AM & v P1RL & P P1WD & P2AM & P2BA P2RL & PBAS PBAS PCON = PCON * PDMF ] PDMF PLOC &
PLOC &
PSZE ` PTYP t PXLB RGNm C RMXV 1 RMdN - RMdV 1 RMdX 6 RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT
7+ RUNT . RUNT
5 Rate ( RunN " " ! S/N% } SCAN 3 SMED
SMLt SMPL T SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ǿ Scal @ Scan 3 TUBE A6 Tmpr < User phAR phCH phDY big phQL c phTR phTR