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V p n c ` Z d Z e p k ^ c c g [ X l R W r ; _ u a e g [ h S a m V M S N j c n Z h m r q N o f ] i e W ` d _ e y M q n b [ j o ` n a Z V ` R V O e ^ a [ c H q b _ S n a V S c c a X g X k ] i h m e r f l Z V k k O ` f b d P h f p a j b j d b i ] \ g ^ g [ R h e ` ^ Y Q l Z ` l g d i Z \ Y [ k s o m r m m ` s S a Z ] c J f _ _ g L K ] f I h c e ] e [ i ^ M [ Y \ N W Q c \ Y _ H e a ] W ` X ` f o _ X _ H k j a b P \ U c a Q ] g e b m q a [ t c h r Y o a j c U a g P i R \ V U N X f c ] ^ N T X V M Q c I S P a S [ ^ Z J N P J M V Y e W f L [ e l ] ^ _ Z a m _ Y P R ^ \ b p f F s h b g V c b ^ a X N f ` l n i V c x [ Y e w g w q w U t t d r n [ n ` j i b e ^ N R V \ \ Y b a f S F E E L U W ` c P G E O : ] E Q P \ P ` O Q U f S = [ M n b _ [ S n X e \ Y S L U Y X V \ E c < _ _ W U U W G c X \ I S g c e o Z [ o V R b \ i l | g q N [ W e ^ [ P m W S R ^ V @ O \ q T f T G a S \ ] V R T U S ` U M D ^ N _ \ K _ i l Y ^ j f T U ] > j b V D ] ^ Z N T d W p T N a k E d V K L Y S e ^ i _ \ p R i X \ Y i k w g X O \ a Y b Y T b R b d V O W n W O Y W X J Z V V c ` P Z ` ` L k X h P M _ ] b H ^ P o a V m n T T s S ` h _ U Y ] o f q | X S ` p j \ i P P c I ^ j t ] g ^ X ^ S ^ x i m Y g a @ y g o \ X j b S Y u U a i ^ ^ V _ U ` I V Z i e Y Z l a c j d ^ Z d W V ^ G e Q R F T P i I R Y L P > ` D B W i { Q ^ f ` d a V T S c m Y b O Q M Q X ` X b R d h \ q ` \ e r n ] j i _ g o _ q S Z g t j P c j V i c M a G b l I d U O E S ` J [ 9 G ` F b \ b b q h e [ ^ e d b ` \ x \ _ [ \ o f f m ^ d n i W Y e W k i ` e ^ V a ` W d M ] \ ] K u Y O e ^ a ` ] Q X X X e [ \ i X P g V j g Z ^ M Y W ` > ` O S T V d Y H r l S b K P d j ` C ^ Z y T h O T ` Z U Z M \ h W d Q I Z I J J b h I R W V ] D W ] S U t N T O P O 5 ] i ] W c h D ^ e Y G R T S T a _ X R ` R m : @ b h R P T I T f M j ^ O O f U p ` j G U Z t s [ U Q f a i k f P H E b ^ M b [ R X t G _ i F h k W c b S \ Y K I b V U j y h i I ] c P [ K ] Q i Z ` c ] _ b f b d f u N h n k e ^ c j d \ R c I j P O j _ \ Y [ _ W \ \ c S c o V S d b W Y j U j Q U R ] H K I W 8 f > ^ a Y \ V Y X ] A \ c Z ` p B ] Q R b W M K g d ] N Y ^ O _ [ ] H T d f \ X N a Q [ T O g ^ X \ ^ [ X e e \ K T o \ ` e h V o b Z \ \ ^ e _ Y c \ R U X k d ` d Z S X T S b [ V ^ F Q L [ K \ q I u U X h T K c M F P b b f U n _ ` k b b [ b ] c ^ \ ` Y V g w c t ` l o Q i a f f m ` w T m U ^ g c U T m [ Z [ B M C ` u \ y a X k e j \ c y t Y T W i h ` \ L N S Q ] ^ \ f L P V I G Z @ a _ O S ` J g i ` M a i c h j x [ j ~ Q Y u f [ ^ f | S ] [ a d ^ P Y U Z o a ^ k Z j a f f U U T Z V Y Y M _ Z R K A V K Q ` a _ R V Q m ^ i \ S b N P X g e ^ | d i a K _ _ ] e f N w q M b r d b k l o c ` T U B T g K e R x B K b _ N N b P \ K X P M K W K N o b Z l [ R N d W ] \ M W S F _ o W Y e \ d ; F ` Y \ e n Y W k N W K B R f ^ o d k Z w t P f O U Q c k I f ^ p o \ Q X P T _ d f k O j ^ H \ a A E P X @ ] O O H k \ F _ M P a M h h u p l g [ L S W f a d h d s c L W k [ k V x T U [ ^ V W b ] S M ^ e V N a ^ Y c R Y n = [ f S M S n O I Y L N ` \ e W H l f V T [ g O S _ ] k V i Q E L C [ H L L S T a d N P U Y i ] M k E V _ f c e U _ g [ i T J Y k t h X A ] q W J I M \ a R i ? i 6 _ j \ h V R ] S Y U e Z Y c [ T S W V W M X e \ v m ] L o f \ [ R ` Y L O f Q c V a d X c b \ m O C E D R \ Z P ` T W a ? 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d ,,,III^^^{{{&&&/99DDDNNNaaavvv ***333>>>GRRGRRRRRRRRGRRGRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR B!.2444433210/.-+*(%%! ] ] ] S S S v v v """..699CCDNONYYXbbbllkvuu}}} ! ! ! " " " # # # # $ $ $ % % % & & & & ' ' ' ( ( ( ( ) ) ) * * * * + + + + , , , , - - - - . . . . / / / / 0 0 0 0 0 1 1 1 1 2 2 2 2 2 3 3 3 3 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 : : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 75d6071cccdc45ad97fc2a3c64b42bf4Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-19_09-47_0524 3130Seq 12244 12244_Costion_K1356_matK_F jmadera 96-Well K1356_F3KB_3730_POP7_BDTv3.mobSeqPop7z
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02 M &$Z X%K *>z xI w)Vw% &$m \ibe6Ctb pAGAATTTTTTGGAAAACGCACATTGAAGAACAGGTGTGGGGAGCTCCTCAATCCAAGCCCCACCGAATCCCCCTTTTTGAAGACTTCCTTTGCTAAAAGGGAATGTCTCCCCCCTAGAAAGAGGCACGGCCCCTCCGCTAGTGTCGAGGGTTGGATGGTCCTACCACAGAAGAGCGAGGTTTATCCGTCTCAATTCTTAAGAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-19 11:34:27 -04:00AEPt 3 AEPt 3 APFN V APXV 2 APrN ȏ APrV 1 APrX } } Ș ARTN ASPF ASPt u ASPt u B1Pt t B1Pt t BCTS BufT d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 0 DATA DATA DATA # G _ DATA
# G 3s DATA # G z DATA # G DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! e LNTD 2 LsrP a MCHN MODF Ȇ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL J P1WD z P2AM P2BA B P2RL PBAS PBAS PCON ~ PCON
PDMF ? PDMF PLOC PLOC
PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT !; RUNT
7 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL 6 SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ǧ Scal @ Scan 3 TUBE F3 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN V APXV 2 APrN ȏ APrV 1 APrX } } Ș ARTN ASPF ASPt u ASPt u B1Pt t B1Pt t BCTS BufT d CTID 3 CTNM 9 CTOw T CTTL \ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA 0 DATA DATA DATA # G _ DATA
# G 3s DATA # G z DATA # G DCHT DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ' FVoc ' FWO_ GATC Feat . . ' GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! e LNTD 2 LsrP a MCHN MODF Ȇ MODL 3730 NAVG NLNE ` NOIS P1AM P1RL J P1WD z P2AM P2BA B P2RL PBAS PBAS PCON ~ PCON
PDMF ? PDMF PLOC PLOC
PSZE ` PTYP \ PXLB RGNm + RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT / RUNT !; RUNT
7 RUNT " Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL 6 SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , Ǧ Scal @ Scan 3 TUBE F3 Tmpr < User phAR phCH phDY big phQL c phTR phTR Yxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["[87aFM3xSbhhzlW\|ngtg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLVo X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUW>|4)|0]MhkGUH0S]D~Q%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y\@f=l\\(Y-/H+G)@-mq5FaT%ycX}OuK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6;