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  ]]]SSSvvv   """..699CCDNONYYXbbbllkvuu}}} !!!"""####$$$%%%&&&&'''(((()))****++++,,,,----....////00000111122222333344444555556666677777888889999999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 749b23ac048941a79d7ed7a56202be2bSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-19_09-47_05243130Seq1224412244_Costion_K1356_matK_Fjmadera96-WellK1356_D3KB_3730_POP7_BDTv3.mobSeqPop7zFFFGIKMORX`l{",6>DHHGEDDEGFC=3( tkd_\ZYYYXVQKC<89=DKNONLMQV\`aaabgnu} /^){'6}uCg'X%LcjQ{&M|xrkc^\^bfgc\RIB?@BEED@<7422479972,&" "$'((&$#%(-38<=;83-($! 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""#"0"?"K"V"c"{""""""""""### #-#:#G#S#`#l#y##########$$$!$-$;$G$T$`$m$z$$$$$$$$$$% %%$%2%>%L%Y%f%m%%%%%%%%%%&& &&'&4&B&P&[&h&v&&&&&&&&&&'''!'.';'I'V'b'q'}'''''''''((((*(6(C(P(_(j(w(((((((((() ))&)3)?)L)Y)f)u))))))))))* **)*2*>*H*S*]*h*r*}***********++ ++!+-+8+D+O+X+`+j+u+++++++++,,,,     1:2).*>)6>>/8>>>>>>>>>>>>;1>>;1>>>>>;86>;>>>>>;3>>>>>>>>>>;>>>>66>>;>1>;;>>>>616>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;6;>;>>;>>>>>>66>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>661>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>>>>  2>>>>>>>>>>>>>>>>8>>>>6>>>>>>>>>>>6+>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>:::::1::::::6::::::::::::2:6:::::*:*&:6::2:::::6*:::66::22::31:::3::&::::::::::::3:3:::3..36:::3:3:63:32:3::52:::5)31:036)::34::32::1(/:1*':)25 :436363645*.!41'3.6316)(14%.)) 56**1/4(/63*55!.%*6.# -4%/,#.#)4((1!/ 3-4/'' .&4%51-) !!!#.3+& 3# .33/* &**#..%*.  &+")0/ %.$!&%$. $#%)),..+&0!,!!! *, ! %      1:2).*>)6>>/8>>>>>>>>>>>>;1>>;1>>>>>;86>;>>>>>;3>>>>>>>>>>;>>>>66>>;>1>;;>>>>616>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;6;>;>>;>>>>>>66>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>661>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;>>>6>>>>>>>>>>>>>>>>>>>>>>  2>>>>>>>>>>>>>>>>8>>>>6>>>>>>>>>>>6+>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>:::::1::::::6::::::::::::2:6:::::*:*&:6::2:::::6*:::66::22::31:::3::&::::::::::::3:3:::3..36:::3:3:63:32:3::52:::5)31:036)::34::32::1(/:1*':)25 :436363645*.!41'3.6316)(14%.)) 56**1/4(/63*55!.%*6.# -4%/,#.#)4((1!/ 3-4/'' .&4%51-) !!!#.3+& 3# .33/* &**#..%*.  &+")0/ %.$!&%$. $#%)),..+&0!,!!! *, ! %  (AU'3/ ^&R1W[252ac)F\'WH=VbP>&D#7 _!FKbA\ . RB>;`[Y./H[UQdL]MOR'2_)P>\ba`/"XN5 `4[H3<-C O.@E<S0XS\_.#?OSLS[$DE_T>-YY;LJ&@U]6@87<E1^`Y(!a7YD9B,?^)b"3/#a7_ V&+V:3HK>P]2S6$8@S#3? @SYY^G ^9\]R#149X@ #8=2`S =!FOJ+?OJHGa7_c\#Kb"S$/G 3I%_?d1;#" ('F_ 3\ _"JYGQ\AC1bF[HQ(F%JSK!L@'A -B")\@#Ka'#L K/A* -K?X>&2T O9 \*)80F+R!DU&I7R_%-<ST?FNR(-TK$VR=J PJ&45H&O: aIK? )V(^0/R\96U^X , SK69 0OCWZ\&JGFVLXA+GP1W3RM+E)1 O",!&[[H ,G;4T_"Qd#,3^L+Yc-I /;?(/-175%'CTDY =`WNd = )S', #AR  KQI<F1(5G6 & WP;b34dd /?1 \G_><D-HTQ.:``#Z(B0]O_C970M# U%ZYR=6 Y.EI<=EH!!XUa5TXT%`E_ - I<_LOGA#2#+6JL+CKL;+GRT0F@$2@c D<E^ aBX AD DDB"^ D0^_#9VD>VH 2 H_I\09+:2H,Fd#0ca <RY2 >\2#\\aV,2 YRKNYC,N8! V@\2(94^Y(J,_/b2 58Z >(Y M\0<HK E X0 *K G = Q R])@X-af5A:\fq)9@QKhf'F ,p{,%J{0uk k9'%$1)ENs6hnj&p~z;pNRM6G};92w#h!K:.Gx5IQq.#)RO-D1i 7[v NCqcyT]y'97 ?x!U[' $mb ,6Ojcx-JIK  3$M6c*d;[.5jxB))P  ool|D<&J) !!dp4z=J?Q,yTa UsRGM=$Z T Pbgwq!d+2^*1\qVH3 xIssl %I#o"u6{.Ehnffr nJT{v<Oy%O#7fC' *,8f!lF f$68f>LNLaG ^P>rW7r\1e&33}-pS`+R>xx% 8W| <,GpUj`w+t y}z~}o0]BF-R-gM 2Zq,@59AguMKv2/8A#}K%fn\7Ke3]>UOP  !S gaD%9dkaJ[GC ~vyT@clqB}I/MP4* "-\+IG_"5  V8*m< Ft~R&} K R }g}JF7 @)u}pqWp[?kSqCGJ&?C8DB %#:  * $2/     xx !       $  00 $   +% 7!"0&2(3<'2*!-'$$$,#7."(%-F-*!6,94>&9))#<J644I:3 6E:MKKRJX5BFDD=6HMDDf@>7=O^X]]GWI__r^hS]NM]p{Yda[XjZjjxSnYkX`bsnfvQXlYaxLxwRWr}chqk|r0lqws4|t#mS!mnp"Z( --  &!  9%MMMPTPPPKP3=PPTPPMdqEqqAqMMMMMMMMM2PPPP3MMMMMMMLM+MM*MqlWqMqMqPPPPPPqPPPPPMMM!PMM6\p_pp)appy[Pq;~8JGpZQz7=Qp,I ?X7-~e7MokD3p|w#=O-3l1vV92S1cBg*q uLdQRwE|k,-88;>oiEUz[[T!Z,->Hcv2|<_{dpWpC B+?pg,pwaz4pPi#p/php\8<*4!{pppppPpp+(Spppppppppppp$ 8p8p* ppPp(0n R VMR<xz &;DLQD- .<4-`L#W#  !zL!.&0(E ,0   4r %*!=%$         "       #     '  '     &          0     *  ( "- +&40Huj\)2%&   "&#'#+#," +:#)#$"!%& !    &- %>C-$)3% `^ P .'+4&')4N#43<!a #>*/1-6%"/2MIY)1 $J[B0,,H5%JR9G*PTLj_]:3)IXgb(LQtG:9'BM!E}uvq0l;N'+xHD{ld,R2@[uUilMYbZ)>fsIqdwyr T3({ltS[d]`z$-Zl7e!L_)"u  2Q'r*!V'UG{ 6,o/[ MPpoBF%"G)Gy: "F"u4Zh  tlRU-7ATTTATTTATTTAAAAAGTACGTACACGAATAATATGTAACATATGCAATTATATTCCATTACCCACATCAAGCTCTCCTAAAACGTACTCCGCGAGGGAAAGAGGGATAAATCTCCTCCACTTGAATAATGAATCGCCCTCCACACGAAGAGGCGGGGCAGGGGAGCACCAAGGCGACGGGGGGGTCGGCATTTGCCCAAACGGGGACGGAAACGAGAAGGATCGAGCATCGAGACGGCTCGGGCGGGAGAGTACAAGGATACATCAGTCAAACTCTTCAGGAACTACCCTCTGAAACAAGAAGGACCTTCTGCAAAATCCGCCAAGAGAGTAGATACCTAGAAAGGGTTATTTTTTATTCAAGAGGAGGAGTCCGATAGGGAGAAAATTCCTAGAGTAGGAATGGCATCGGATATTGAAACACACAAGATAAGATATACTTGTTTTTATACACGGCTGAAACGGGATACACTTGTCCTATGAAGTAGGGACAGGTATAATTTCCCCCTTGAATGCCGGGGTCTTAAAGATATGTGTCCGCACCATGCCGGAATATACACATGCTACTAATACACGATTACTTCATCCGCAAAGTACTATATCACACGAGTGTGACACGTTATACCATAGTTACACAGACTCCCACGCGAGGGTACTCAAATATCACATATTACGATAATGAGGATCGTTACACAATATCTACTAACACTTCTCTTAGTATAACAGTCTATGTAAGGTAAAGTATAGCACAATAACATCTTGTGATAATGACTCCATGTACAGCTACTCCTCATACAACTCTAATATACATCTCGGTACGCATATATACAGGTCTCACTCACAATAACACAGCCTCTTCTCGGGCCTTGAGAGCGTGTATCGTATGGTTATATTACATCTGACTCCCCCCTCGTGAATTTAGATATTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-19 11:34:31 -04:00AEPt3AEPt3APFN VAPXV2APrN ȏAPrV1APrX}}ȘARTNASPFASPtuASPtuB1PttB1PttBCTSBufT dCTID3CTNM9CTOwTCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA ,9Xr_DATA ,9XrDDATA ,9XrCDATA ,9XrDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BLIMS!!eLNTD2LsrPaMCHNMODF ȆMODL3730NAVGNLNE`NOISN/P1AM@rP1RL@kbP1WD@yP2AM@bP2BAP2RL@"PBASRPBASNbPCONgPCONd"PDMF?PDMFNFPLOC@\PLOC@UPSZE`PTYP\PXLBRGNm+RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  / RUNT  !;RUNT  7RUNT  "Rate RunN"" S/N%N'SCAN3SMED SMLtSMPL 6SPACAUݗSPACN?SPACAUݗSVER4.0SVER SVER,,ǦScal@Scan3TUBED3Tmpr<UserphARphCHN]phDYbigphQLcphTRphTRsThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN VAPXV2APrN ȏAPrV1APrX}}ȘARTNASPFASPtuASPtuB1PttB1PttBCTSBufT dCTID3CTNM9CTOwTCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA ,9Xr_DATA ,9XrDDATA ,9XrCDATA ,9XrDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab&FVocFWO_GATCFeat//GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BLIMS!!eLNTD2LsrPaMCHNMODF ȆMODL3730NAVGNLNE`NOISN/P1AM@rP1RL@kbP1WD@yP2AM@bP2BAP2RL@"PBASRPBASNbPCONgPCONd"PDMF?PDMFNFPLOC@\PLOC@UPSZE`PTYP\PXLBRGNm+RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  / RUNT  !;RUNT  7RUNT  "Rate RunN"" S/N%N'SCAN3SMED SMLtSMPL 6SPACAUݗSPACN?SPACAUݗSVER4.0SVER SVER,,ǦScal@Scan3TUBED3Tmpr<UserphARphCHN]phDYbigphQLcphTRphTRY#rNT/$4OY ~?p96UI//`JiOzJ} {&kdA[L25nWsyZKh~8JePS"Fp?(J/Hcz,(,y]18`F7IOo_3@/$9E^E:D+UZ~en?R/FIOb]rDKRm*u!?Xa./l3672_4&blIk(!Y`%]RI1]f\+!@rIwYWgUTHG&/Bc1h67A"yV+L"o&Hmd1I*w&OLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOj1 *>kId|CHp{kG5KF4-L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/`VWY+a:WkeC.%oh^5Q1V?|&@8je]i^mc:=@tWWU -8/bro}#Tv4*un,T(k"T'ogLOE2(/(W,XD3J6EX! w+[_3sh(j#1>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#