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Z Z Z Q R R s s s %%%0/4::CEENOOXXXaaajjjsss|{{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9fdb50d767c34c46ad25ec761010354aSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-13_22-08_0517 3130Seq 12232 12232_Costion_K1355_matK_R jmadera 96-Well K1355_G7KB_3730_POP7_BDTv3.mobSeqPop7z ",=Un ';LX\XPKPb P|3GWdnodI ~]KE?3J}ocT>"
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K ^ O _ x c t v N Y ~ = G & \ u o p z 9 A p D Y xG '| d 4 z OqD H 3' u 5 @ ; w \ I B r ~I G;ATTTATTTACGACTCCGAGAAAAAGAATAATAAGTATTAGCACCTTCTTGAAAGACTTGGACGTACCTTATACATAATGGCCTGTAAGTGTATCAGGCGGAAATGTACGGCTACTCATAATGGTCTTACGAAAACAGACGATGCCCTAGGGATCCGGTGACGAGGAGCGGCCTCTCCGGATGGGGGTGACGAAAGTGGGGTAAAACCTCGCTACTAGCCAGAATAGAGGAGGAAGGAGTTGCGCGTGAAATAACGTCACTGTGTGGCGTAGTCCCCCTCAGGGGTTATCACTCCTGGAATGGGGAGGACGCTAAGATGAGTAACATAGAGCGATCCATTGCAGGGCAGAACCCATAAGGGGAAGACACTGTGATGGACAGACGGGGTAATACCTTCAACGAAGCATGGGGAAAAATGTCCTCGAAGTCTTCAGTATTTACCCTTCGTGATATATCACAGGAAAGAAGGGAATATTCGGGGAAGAGGGGGAGCGCCTCCCCCCACTCATGTCGTAGCCATCAACGATAAAGTCCAACGACACAGAGGCTCTTAATAGCTCCGGCGGGACTCCGTTATAGGTTGATTGTTAAAGAGAATACGTGATATGGAGTTGGAGGGATAGATAATTATACTTTCACATATATGTAATATAGTAGTATGACGTTTATCGTGTACTTCCCATGCTGGGGGGTATAGGTAGATAGCCAGGTAGAATGTGGACAATACTACTCTCAGTAGTATTATCCCTCCACTTTCATTATTTATGGGAGGGAGACATCTCCATCTTCTATATCGGAATAGGAGCAACGATACCTACATATATATAAGATACTGTACTCATATATGAGACATAATAGAGTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-13 23:40:13 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS j BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA (r P DATA
(r P 5 DATA (r P DATA (r P w DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 3 LAST ~ ~ h} LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS (c P1AM ] J8 P1RL ] C~ P1WD ] P P2AM ] ^f P2BA ] ] e P2RL ] W PBAS ] ] + PBAS ] ] ( PCON ] ] @! PCON ] ] < PDMF PDMF (z PLOC ] 6
PLOC ] /P PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 3 RUNT '
RUNT , RUNT % Rate RunN " " S/N% ([ SCAN 3 SMED
SMLt SMPL SPAC AdS SPAC (s SPAC AdS SVER 4.0 SVER i SVER , , Scal @ Scan 3 TUBE G7 Tmpr < User phAR phCH ( phDY big phQL c phTR phTR 1Z This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS j BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA (r P DATA
(r P 5 DATA (r P DATA (r P w DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab xa FVoc xP FWO_ GATC Feat / / x! GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 3 LAST ~ ~ h} LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS (c P1AM ] J8 P1RL ] C~ P1WD ] P P2AM ] ^f P2BA ] ] e P2RL ] W PBAS ] ] + PBAS ] ] ( PCON ] ] @! PCON ] ] < PDMF PDMF (z PLOC ] 6
PLOC ] /P PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 3 RUNT '
RUNT , RUNT % Rate RunN " " S/N% ([ SCAN 3 SMED
SMLt SMPL SPAC AdS SPAC (s SPAC AdS SVER 4.0 SVER i SVER , , Scal @ Scan 3 TUBE G7 Tmpr < User phAR phCH ( phDY big phQL c phTR phTR [4\QWuMVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&*L!b34pU,\5az9\ %EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHP)$zt)e|}M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3udrVbH`6@m r;%`,vfR`z#`:L{|.&kGz9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*+RB^:}JKp]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0